• Title/Summary/Keyword: 부착과 제거

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Improvement of an hydroxyapatite bead adherence assay for streptococcus sanguis (Streptococcus sanguis의 구형 Hydroxyapatite 비드에의 부착 Assay 방법의 개량)

  • 최선진;이시영;송요한
    • Korean Journal of Microbiology
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    • v.27 no.1
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    • pp.48-55
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    • 1989
  • The purpose of the present investigation was to improve several procedures being used in the adherence assay of Streptococcus sanguis cells to hydroxyapatite (HA) beads and to study the effect of the beads on the counting of radioactivity. The standard adhere assay involved the adherence of radiolabeled bacteria to 40mg of HA beads. The beads were mixed with ['H]thymidine-labeled bacterial cells and incubated for 60 minutes at room temperature. Unadsorbed cells were removed, the beads with adsorbed cells were dried, and the radioactivity was monitered in a scintillation spectrometer. The 30 seconds sonication of cells in a form of long chains appeared to be adequate for obtaining mostly singlet or doublet cells. Unlike the counting of S. sanguis cell suspension, bacterial cells adhered to HA or saliva-coated HA(SHA) required smaller volume (2.5ml) of scintillaton fluid for better counting. Eighteen percent quenching of counts could be attributed to the beads. Among 3 procedures commonly used to equilibrate the beads for adherence assay, no differences were found in their effectiveness. The HA beads on which the bacteria remained attached in scintillant during the counting were found to be the source of sample self-absorption representing 34.5% of the total radioactivity counts resulting from the beads dissolved in HCl solution.

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Effects of Catalase and Cumulus Cells during In Vitro Maturation in Porcine Oocytes (돼지난자의 체외성숙시 Catalase와 난구세포의 영향)

  • Lee, S.Y.;Sa, S.J.;Kim, K.J.;Cheong, H.T.;Yang, B.K.;Park, C.K.;Kim, C.I.
    • Korean Journal of Animal Reproduction
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    • v.23 no.3
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    • pp.239-245
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    • 1999
  • The effect of catalase on in-vitro maturation in porcine oocytes with or without cumulus cells were studied. The maturation rates were not significantly different in medium with and without catalase during in vitro maturation of oocytes. However, the maturation rates of oocytes with cumulus cells were significantly higher (P<0.05) than in oocytes without cumulus cells regardless of the presence of catalase. On the other hand, the maturation rate in oocytes cultured with cumulus cells for 48 h (57%) was significantly (P<0.05) higher than in oocytes with cumulus cells for first 24 h period (42%) only. In another experiment, the maturation rate was significantly (P<0.05) higher in medium containing catalase for last 24 h period only than in medium containing catalase for first 24 h period during in vitro maturation of ooytes with or without cumulus cells. But the oocytes matured to M-II stage were observed at 24 h of culture of oocytes without cumulus cells only. When oocytes with cumulus cells were cultured for 72 h, the maturation rates was significantly (P<0.05) higher in medium with (79%) than without (65%) catalase during in vitro maturation. These results indicate that cumulus cells are necessary for in vitro maturation of porcine oocytes, catalase have effect according to the addition periods and can prevent aging of porcine oocytes during maturation in vitro.

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Vascularized clavicle을 이용한 기관재건술

  • 정동학;김영모;조정일;윤정선
    • Proceedings of the KOR-BRONCHOESO Conference
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    • 1997.04a
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    • pp.117-117
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    • 1997
  • 성문하 협착과 기관 협착이 동반된 경우 이를 치유할 몇가지 방법이 시도되고 있으나 만족할만한 효과를 얻지는 못하고 있다. 저자들은 성문하와 기관협착이 동반된 27세 남자환자에서 기관 및 성문하 협착부 전면을 제거후 쇄골에 흉쇄유돌근(Sternocleidomastoid muscle: SCM)이 붙은채로 쇄골의 절반을 절제하여 수질부위를 제거후 협부점막을 부착하였다. 이렇게 얻어진 Vascularized clavicle을 성문하와 기관의 전면부 재건에 이용하여 좋은 결과를 얻었기에 문헌고찰과 함께 이를 보고하고자 한다.

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RBC 운전조건 변화에 따른 생물막의 형성 및 조성 변화 특성

  • 최정순;남귀숙;박근태;손홍주;이상준
    • Proceedings of the Korean Environmental Sciences Society Conference
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    • 2001.05a
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    • pp.162-163
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    • 2001
  • 기질농도, HRT, 온도, pH, $Ca^{2+}$ 농도변화에 따른 반응기의 생물막형성과 세포외고분자물질의 조성변화에 따른 기질 제거율을 살펴보았다. 그 결과 반응기의 초기 생물막 형성은 각 조건에 따라서 약간의 차이를 보였다. 반응기 운전 초기에 균에 의해 생산된 세포외고분자물질은 점액질의 형태로 세포벽에 부착되어 주위환경의 해로운 요인으로부터 세포를 보호하는 기능을 가지고 있어 생물막 형성을 촉진시키는 역할을 하였으나, 생물막이 안정화된 후에는 오히려 생물막의 산소투과와 영양분의 접촉을 떨어뜨리는 역할을 하여 기질 제거율을 저하시키는 작용을 하는 것으로 나타났다.

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Analysis of Attached Bacterial Communities of Biological Activated Carbon Process Using DGGE Method (DGGE 기법을 이용한 생물활성탄 공정의 부착 박테리아 군집분석)

  • Son, Hee-Jong;Choi, Jin-Taek;Son, Hyeng-Sik;Lee, Sang-Joon
    • Journal of Korean Society of Environmental Engineers
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    • v.34 no.8
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    • pp.533-540
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    • 2012
  • The concentration of organic compounds was analyzed at each step of BAC (biological activated carbon) process though BDOC (biodegradable dissolved organic carbon) total/rapid/slow. Further, bacteria communities and biomass concentrations measured DGGE (denaturing gradirnt gel electrophoresis) and ATP (adenosine triphosphate) methods were analyzed. The bed volume of steady state is different based on assessment of organic compounds removal. Bed volumes at steady state in DOC, $BDOC_{rapid}$ and $BDOC_{total/slow}$ removal were around 27,500, 15,000 and 32,000, respectively. A biomass didn't change after the bed volume reached 22,500 according to analyzing HPC (heterotrophic plate count) and ATP concentration of bacteria. The concentration of HPC and ATP were $3.3{\times}10^8$ cells/g and $2.14{\mu}g/g$, respectively. The number of the DGGE band were only 5 at the bed volume 8,916, but increased up to 11 at the bed volume 49,632. As operation time increase, bacterial group were more diversity. Four bacteria species including Pseudomonas fluorescens, the uncultured bacterium similar to Acinetobacteria, uncultured Novosphingobium sp. and Flavobacterium frigidarium have detected from the early stages and Proteobacteria group were dominantly detected.

Computational Fluid Analysis for the Otter Boards - 4 . Efficiency Analysis of the Cambered Otter Boards for the Vortex Generators - (전개판에 대한 수직해법 - 4 . 과발생기에 따른 만곡형전개판의 성능분석 -)

  • 고관서
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.27 no.4
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    • pp.286-292
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    • 1991
  • The authors propose to use the vortex generators in order to improve of the efficiency for the cambered otter boards. The equipments and testing method of this model test was the same as the previous report. This study was tested for 6 models such as the single cambered, the V-shaped cambered and the slotted cambered otter board without and with vortex generators. The results obtained are as follows: \circled1 C sub(L) of the single cambered model otter board with vortex generators was increased about 10% in comparison with that of model without vortex generators, C sub(D) decreased 2%, and L/D increased 5~20%. \circled2 L/D of the V-shaped cambered model otter board with vortex generators was increased 10~20% in comparison with that of model without vortex generators. \circled3 C sub(L) of the two slotted cambered model otter board with vortex generators was increased about 20% within an angle of attack 25$^{\circ}$ in comparison with that of without vortex generators, C sub(D) increased 5~20%, and L/D was higher than prototype within an angle of attack 20$^{\circ}$. \circled4 The separation point of the model otter boards with vortex generators was removed back ward a little in comparison with that of the model without vortex generators. \circled5 Flow speed difference of the back side to the front side of model otter boards with vortex generators was increased a little in comparison with that of the models without vortex generators. \circled6 The size of separation zone in case of the model otter boards with vortex generators was decreased about 10% in comparison with that of the models without vortex generators.

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Structural Dynamic Modification of Fixture using Antiresonance Frequency Analysis (반공진 진동수 해석에 의한 치구 설계 변경)

  • 김준엽;윤을재
    • Journal of the Korean Society of Propulsion Engineers
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    • v.1 no.2
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    • pp.48-57
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    • 1997
  • The method of antiresonance frequency analysis of multi-input system is proposed. The structural dynamic modification using antiresonance frequency analysis is also applied to reduce the undertest at specimen attachment points on the fixture in environmental vibration test, which is resulted from the inconsistency of antiresonance frequencies. Several computer simulations show that the proposed method can remove the undertest problem which is not removed in conventional vibration test control. And the effectiveness of the method is verified with the impact hammer excitation of aluminium fixture model.

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Fixation Adsorption Control system for Film of Glass (유리기판 고정 압착 제어시스템)

  • Kim, Gwan-Hyung;Oh, Am-suk;Kwon, Oh-Hyun;Kim, Song-Hyun
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2014.05a
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    • pp.913-914
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    • 2014
  • PE/PET 필름이 부착된 디스플레이 또는 유리기판 가공에 있어서 유리기판에 대한 고정은 필수 기술요소라 할 수 있다. 이러한 유리기판 위의 필름을 제거하기 위해서는 정밀한 절단기술이 필요하며, 평탄도가 일정하지 않는 유리기판에 대하여 균일한 압력을 가하여 일정한 평탄도를 유지하여 유리기판에 부착된 필름을 가공할 수 있다. 이러한 유리기판에 부탁된 필름을 가공할 수 있는 기술은 유리기판을 흡착시키는 공압기술과 정밀가공처리된 평탄도 $5[{\mu}m]$이상의 베이스(base)판 위에 유리기판을 고정할 수 있는 고정 압착시스템을 제안하고자 한다. 본 논문에서는 유리기판이나 표면의 부착된 필름커팅 시 유리기판위에 결함이 발생하지 않도록 유리기판을 흡착시키는 공압시스템 및 정밀가공시스템을 제안하고자 한다.

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Improvement of Medium and Small Urban Stream Water Quality and Applicability of Design Factor Using Biological and Physicochemical Processing (도심지역 내 중·소하천 수질 개선을 위한 가압부상 및 관로형 미생물 부착 공정 적용에 관한 연구)

  • Kim, Moon-Ki;Choi, Jung-Su;Kim, Sam-Ju;Kim, Hyun-Gu
    • Journal of Korean Society of Environmental Engineers
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    • v.35 no.7
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    • pp.509-517
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    • 2013
  • The purpose of this study is to assess the applicability of device-type stream coagulation process which combines physiochemical, biological processing for efficient improvement of water quality in small, middle-sized urban streams. The stream purification facility of this study is compose of pressure flotation type Micro Bubble Process(MBP) to remove TSS and TP and conduit line type Attached Microbial Pipe System(AMPS) to remove BOD. Test conditions of each device were set by floating stay time and change of ultra fine bubble injection amount of MBP, and change of AMPS stay time. Also, removal efficiency of pollution sources of each process were assess by change of season. As result of continuous operation of each process, MBP showed a maximum of TSS 83.69%, TP 95.15% process efficiency and AMPS showed a maximum of 52.95% TBOD5 removal efficiency. Also as result of circular operation of each process, MBP showed a maximum of TSS 69.75%, TP 70.17% process efficiency and AMPS showed a maximum of 68.58% TBOD5 removal efficiency. Therefore, it is considered that this stream coagulation process is effective in improving the water quality of streams in urban areas.

Treatment of dyeing wastewater using Moving Bed Bioractor (부유메디아 생물막 공정을 이용한 염색폐수처리)

  • Shin, Dong-Hoon;Lee, Sang-Hun;Ryu, Seung-Han;Park, Jun-Hyung;Jo, Seog-Jin
    • Proceedings of the Korean Society of Dyers and Finishers Conference
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    • 2011.03a
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    • pp.110-110
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    • 2011
  • 염색공업 폐수는 그 성분이 일반적으로 매우 복잡하며, 작업공정의 가동 사항에 따라 수질 변동이 큰것이 특징으로, 각 공정에서 배출되는 염료, 보조화학물질, PVA(Polyvinyl alchol), 전분, wax 등이 포함되어 있으며 pH가 높고, 색도로 인해 하천에 방류될 경우 확산성이 높아 미생물에 의한 자정작용을 방해하여 하천의 수중생태계를 파괴할 우려가 있다. 이러한 염색산업에서 발생하는 폐수는 일반적으로 응집침전, 부상분리법 등의 전처리한 후 활성오니공정으로 처리하는 방법이 널리 이용되고 있으나, 이들 처리공정으로는 폐수 속에 포함되어 있는 다양한화학적 구조의 색소성분 및 유해물질을 완벽하게 제거하는 것이 어려운 실정이다. 유기물 함량이 높은 염색폐수를 처리하기 위해 제안된 기술로는 산소활성슬러지법, 유동상 및 고정상 생물막법, 포괄고정화법 등이 있다. 이러한 기술들중 기존의 처리공정을 증축없이도 처리효율을 높일 수 있는 방법으로 담체를 이용한 부유메디아 생물막공정(Moving-Bed BioReactor, MBBR)이 있다. 이공정은 미생물이 부착, 성장할 수 있는 공극율과 비표면적이 큰 담체를 이용하므로 반응조내의 부유 미생물 뿐만 아니라 담체에 고농도로 부착된 부착 미생물에 의해서도 유기물을 제거하기 때문에 다른 공정들에 비해 처리효율이 뛰어나고 기존의 활성슬러지 공정에 비해 갑작스러운 부하변동 및 유독성 폐수유입에 대해서도 안정적으로 운전이 가능한 장점이 있다. 본연구에서는 부유메디아 생물반응기(Moving-Bed BioReactor, MBBR)을 이용하여 염색폐수내 $COD_{Mn}$, 색도 및 난분해성 물질인 PVA 저감에 대한 Lab-scale test 수행하였다. 실험에 사용된 염색폐수의 수질은 평균 pH 13, $COD_{Mn}$ 900 mg/L, SS 135 mg/L, 색도 1,134 [C.U.], PVA 593 mg/L였으며, 2L의 반응기를 사용하여 회분식 실험을 수행였다. 본 실험에서는 호기성 미생물에 의한 염색폐수의 생분해가 유지되는데 필요한 최적의 용존산소 농도와 이에 필요한 공기 폭기량을 결정하기 위하여 i) DO uptake rate측정과 ii) 담체의 충진율, iii) COD/N ratio, iv) Air 유량, v) 담체내 흡착제의 종류, vi) $Ca^{2+}$ 첨가가 염색폐수의 생분해에 미치는 영향을 살펴보았다. 운전시간을 7일로 하여 COD, 색도, PVA 등을 측정한 결과 담체를 첨가한 경우가 담체를 첨가하지 않은 경우 보다 제거효율이 뛰어났다. 특히 충진율 30%(C/N 3)의 경우에서 COD, 색도, PVA의 제거율이 각각 평균 65%, 70%, 60%로 가장 높은 제거효율을 나타내었다.

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