• Korean Journal of Microbiology
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• v.31 no.4
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• pp.267-273
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• 1993

We screened promoters inducible by superoxide radical from Escherichia coli. For this. we constructed random promoter library from E. coli MG 1655 using a promoter-probing plasmid. pJAC4. Six hundred and sixty clones in this library were classified based on their promoter strength by ampicillin gradient plate assay. Three hundred and eighty three clones with relatively weak to medium promoter strength were selected and then screened for their inducibility by superoxide radical on ampicillin gradient plate containing paraquat. Three clones (clones 5. 15 and 34) were detected to be induced by paraquat treatment and the level of induction were between 1.4 and 4 folds. Comparison of nucleotide sequences of the cloned promoter fragment with registered sequences in GENBANK and EMBL databases suggests that the cloned DNA fragments have not been yet characterized in E. coli. Transcription start sites in these clones were determined by rrimer extension and S I nuclease protection analysis. S 1 analysis of clones 5 and IS indicated that the mRNA levels were increased by paraquat treatment. Especially. clone 5 \vas found to have two transcription start sites. the upstream start site of which was selectively used by paraquat treatment. Searching for promoter clements. we found that only the downstream promoter of clone 5 has -10 and - 35 promoter elements recognized by RNA polymerase ($E\sigma^{70}$) and the others have no conserved promoter elements. This suggests that these superoxideinducible promoters may require transcription initiation protein(s) other than $E\sigma^{70}$.

• Journal of Internet Computing and Services
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• v.2 no.2
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• pp.71-93
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• 2001

Commonly to design a database system. a conceptual database has to be designed and then it is transformed into a logical database schema prior to building a target database system. This paper proposes a method which transforms a Temporal Entity-Relationship Model(TERM) into a Temporal Object-Oriented Model(TOOM) to build an efficient database schema. I formalize the time concept in view of object versioning and specify the constraints required during transformation procedure. The proposed transformation method contributes to getting the logical temporal data from the conceptual temporal events Without any loss of semantics, Compared to other approaches of supporting various properties, this approach is more general and efficient because it is the semantically seamless transformation method by using the orthogonality of types of objects, semantics of relationships and constraints over roles.

• Journal of Life Science
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• v.17 no.2 s.82
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• pp.272-278
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• 2007

Proteases are enzymes that break peptide bonds between amino acids of other proteins and occupy a crucial position with respect to their applications in both physiological and commercial fields. In order to screen new source of protease, bacteria producing extracellular proteases at low temperature were isolated from deep sea water of East Sea, Korea. A bacterium showing the best growth rate and production of an extracellular protease at low temperature was designated HJ 47. The DNA sequence analysis of the 16S rRNA gene, phenotypic tests and morphology led to the placement of this organism in the genus Pseudoalteromonas. Although maximal growth was observed at $37^{\circ}C$, enzyme production per culture time was maximum at $20^{\circ}C$. At this temperature, extracellluar protease production was detected from the end of the exponential phage to stationary phase, and maximal at 15 hours after initial production. The optimum temperature and pH of the protease were found to be $35^{\circ}C$ and 8.

• The Journal of the Korea Contents Association
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• v.15 no.2
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• pp.552-566
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• 2015

This paper deals with suicide that has become a serious problem in our society as well as in the world today from a Christian perspective. Historical position regarding suicide is divided into two; some see it as a brave and honorable act and give approval to it, the others view it as act of giving up the life that is given by God and are opposed to it. Though suicides in the Bible are mostly committed to maintain honor, because of Augustin's position it became negative in Christianity, leading to a thought that one who committed suicide goes to hell. Why then people commit suicide? The views of scholars on the cause of suicide are divided into three; biological, psychological, sociological. My position is that though each or all of them contribute to suicide, yet the decisive factor is on how a person reacts to them. Major factor of suicide is how humans as interpretive beings accept the present despair. On the basis of this understanding of suicide and humans, I conclude this paper with three new recognitions as an essential answer to suicide. First, humans are made in God's image and belong to Him, thus their life is precious. Second, Christianity views humans as ones look for the hope in the future as the eternal life. Third, suicide is a violation of the commandment 'do not kill' yet it is not an act of sin but a disease that needs to be cured. To achieve this Christianity must not only spread the idea of respect for life and also run suicide prevention center every city.

• 한국지구과학회:학술대회논문집
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• 2010.04a
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• pp.22-22
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• 2010

우리들은 초 중등교육을 통해 암석의 종류와 특징, 지질형태와 퇴적구조, 그리고 화석에 대해 지속적으로 교육 받아왔다. 그러나 암석이나 노두를 보고 학교에서 배운 지식을 바르게 적용할 수 있는 학생은 매우 드물다. 또한 아름다운 경관을 보며 지질학적으로 형성과정을 해석할 수 있는 사람도 매우 드물 것이다. 여기서는 지질경관에 대한 정보를 어떻게 제공하고 있는가에 대한 연구 성과를 정리해보고 문제점을 도출하고자 한다. 현재까지 지질경관을 알려주는 연구 성과를 크게 나누면 3종류가 있다. 첫째, 지질학습장의 개발 및 활용에 관한 연구이다. 이 부류의 수집된 자료는 학위논문(29편)과 전문학술지(27편)로 현재 56편이나 검색에 따라 더 늘어날 것이다. 이들은 야외 노두에 대한 효과적인 지질학습을 위한 자료개발을 위주로 하고 있다. 이러한 내용들은 전문가 양성에는 대단히 좋은 프로그램이지만 청소년이나 일반대중들에게 너무 어려운 내용으로 구성되어 있다. 또한 교육청에서 주관하는 탐방계획으로 "경기도 지구과학교사 연구회"가 있는데, 주로 경기도 한탄강유역을 대상으로 하고 있다. 이 연구회 자료에서는 지역의 특성을 살린 야외지질학습장을 개발하고 현장에 적용하기 위한 노력을 볼 수가 있다. 두 번째로 과학전람회 자료로서 현장교사와 학생들의 야외학습에 대한 인식이 잘 나타나 있다. 수집된 자료는 총 55편으로 야외지질조사를 통해 관찰 자체에 중점을 두었는데, 최근에는 노두관찰에 덧붙여 탐구학습 태도와 창의성을 함양하는 쪽으로 옮겨가고 있다. 세 번째 자료는 일반대중을 위한 서적이다. 이 서적들은 저자들의 전공에 따라 그 내용이 다소 다르다. 즉 일반여행안내 책자는 노두나 암석은 소개하고 있으나 지질학적 설명이 전혀없으며(예, 남도해안 2000리), 지리학자들의 저술에는 지형의 형성을 지질학적으로 설명하려 애쓴 흔적이 보인다(예, 한국지리이야기). 다음으로 지구과학들의 저술은 전형적인 지질답사 안내서에 가까워 내용 자체는 좋으나 흥미가 다소 떨어진다(예, 가족이 함께 떠나는 지질여행, 제주도 지질여행). 지질학과 흥미를 적절히 혼합한 책으로 '우리 땅 과학 답사기'정도가 있다. 최근 한국암석학회에서는 "우리의 명산"을 지질과 경관의 관점에서 해석하려는 심포지엄을 2차례 가졌다. 그러나 그 내용면에서는 경관지질이라는 개념을 정립했다고 보기에는 다소 미흡 하나, 황상구 교수의 주왕산에 관한 내용은 앞으로 경관지질 연구에 대한 발전의 여지를 보여주었다. 앞으로 지질경관에 대하여 지질학적 야외답사나 과학지식 습득에 국한시키지 말고, 그 지역의 특성을 살린 관광 또는 민속과 연계하여 지구과학적 학습효과와 더불어 인성교육에 도움이 되는 학습 콘텐츠를 개발하도록 노력해야 할 것이다. 일반 대중이 우리나라의 빼어난 지질 경관을 손쉽게 이해하고 즐기게 되면, 세계자연유산이나 세계지질공원을 지정 받는 과정도 더 쉬워질 것이다.

• Journal of KIISE:Software and Applications
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• v.29 no.9
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• pp.676-683
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• 2002

The success of XML(eXtensible Markup Language) is primarily based on its flexibility : everybody can define the structure of XML documents that represent information in the form he or she desires. XML is so flexible that XML documents cannot be automatically provided with an underlying semantics. Different tag sets, different names for elements or attributes, or different document structures in general mislead the task of classifying and clustering XML documents precisely. In this paper, we design and implement a system that allows checking the semantic-based similarity between XML tags. First, this system extracts the underlying semantics of tags and then expands the synonym set of tags using an WordNet thesaurus and user-defined word library which supports the abbreviation forms and compound words for XML tags. Seconds, considering the relative importance of XML tags in the XML documents, we extend a conventional vector space model which is the most generally used for document model in Information Retrieval field. Using this method, we have been able to check the similarity between XML tags which are represented different tags.

• Korean Journal of Environmental Biology
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• v.27 no.1
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• pp.88-94
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• 2009

The process of atherosclerosis begins through secretion of inflammatory cytokine or adhesion of leukocyte from damage in blood vessels and transmigration. This study was conducted to investigate the effects of delphinidin chloride (DC) in the initial process of atherosclerosis on the expression of ICAM-1 (Intracellular Adhesion Molecule-1) and VCAM-1 (Vascular Adhesion Molecule-1) related to adhesion of leukocyte at the HUVEC (human umbilical vein endothelial cell line. As a result, cell growth inhibition rate at 50 ${\mu}M$ was respectively 4, 3 and 5% without cell toxicity. As a result of morphological observation monocyte-endothelial cell adhesion assay and optical microscope carried out to measure attachment of mononuclear cells to endothelial cells induced by Tumor necrosis factor-alpha (TNF-$\alpha$) at concentrations without cell toxicity, DC concentration-dependently suppressed attachment. When effects on the expression of VCAM-1 and ICAM-1, cell adhesion molecules induced from endothelial cells by TNF-$\alpha$, were comparatively analyzed using western blot analysis and RT-PCR methods, protein of VCAM-1 and ICAM-1 and expression at the level of mRNA were concentration-dependently reduced. Taken together, the results of this studies provide evidence that DC possess an anti-metastatic activity.

• Journal of Physiology & Pathology in Korean Medicine
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• v.21 no.3
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• pp.583-590
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• 2007

The significance of Han medicine (韓醫學), the Korean traditional medicine, that has lasted throughout the past couple millenniums relies upon Han Philosophy distinguished by its uniqueness. In brief, the specificity of Han medicine is characterized by unity of spirit and body, part and whole. According to this theory, when curing a frozen shoulder, it is usually cured by acupuncturing the area around the part that aches, but also doing so on the area that is totally different from the aching part such as the opposite part of the body. In fact, this can be pursued only through aspects that enable one to realize the unity of part and whole, and a ground for this possibility bases upon the crux of Eastern Philosophy, I-ching(역), such as theory of Five Elements (음양오행) and Three Pillars(삼재). In Western set theory, the issues of Class(부류) and elements(요원), whole and part were independently discussed in the area of mereology, and the question of part and whole was encountered with aporia and paradox since Greek ancient philosophy. At the turn of this century, many philosophers endeavored to pursue academic inquiry to resolve this paradox, especially by Russell and Whitehead through ${\ll}$Principia Mathematica${\gg}$ at the beginning of this century. in the process, there came out a phrase 'Russell's Paradox'. Russell himself proposed a typological resolution as an answer to the inquiry. However, 'Russell's Paradox' still remains as an aporia even till present days. During medieval period, this inquiry was even considered as 'insolubia'. Throughout this paper, 1 attempt to provide an analytic aspect on 'Russell's Paradox' from an unique thinking method and perspective of Han medicine that embodies the concept of 'unity of part and whole'. To do so, 1 suggest a physiological model in the first place depicted by diagrams of Circle 원, Quadrangle 방, Triangle 각(CQT) that portray the logic of Hado or Hotu 하도 which is 'the pattern from the river Ho'. That is to suggest that CQT원방각 of Hado can De a logical foundation that explains the notions of spirit (정신,뇌), internal organs(장부), and meridian system which functions as a solution to the question of 'Russell's Paradox'. There are precedent academic works examining the issue from philosophical aspect such as Sangil Kim's ${\ulcorner}$Han medicine과 러셀역설 해의${\lrcorner}$ Han Medicine and Resolution of Russell's Paradox(2005), and this analysis will further attempt to critically examine such works from a perspective of Han medicine.

• Applied Chemistry for Engineering
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• v.25 no.1
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• pp.1-13
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• 2014

Lithium ion batteries (LIBs) are the state-of-the-art technology among electrochemical energy storage and conversion cells, and are still considered the most attractive class of battery in the future due to their high specific energy density, high efficiency, and long cycle life. Rapid development of power-hungry commercial electronics and large-scale energy storage applications (e.g. off-peak electrical energy storage), however, requires novel anode materials that have higher energy densities to replace conventional graphite electrodes. Germanium (Ge) and silicon (Si) are thought to be ideal prospect candidates for next generation LIB anodes due to their extremely high theoretical energy capacities. For instance, Ge offers relatively lower volume change during cycling, better Li insertion/extraction kinetics, and higher electronic conductivity than Si. In this focused review, we briefly describe the basic concepts of LIBs and then look at the characteristics of ideal anode materials that can provide greatly improved electrochemical performance, including high capacity, better cycling behavior, and rate capability. We then discuss how, in the future, Ge anode materials (Ge and Ge oxides, Ge-carbon composites, and other Ge-based composites) could increase the capacity of today's Li batteries. In recent years, considerable efforts have been made to fulfill the requirements of excellent anode materials, especially using these materials at the nanoscale. This article shall serve as a handy reference, as well as starting point, for future research related to high capacity LIB anodes, especially based on semiconductor Ge and Si.

• Journal of Yeungnam Medical Science
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• v.18 no.1
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• pp.39-50
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• 2001

Background: Power spectrum analysis method is a powerful noninvasive tool for quantifying autonomic nervous system activity. In this paper, we developed a data acquistion system for estimating the activity of the autonomic nervous system by the analysis of heart rate and respiratory rate variability using power spectrum analysis. Materials and methods: For the detection of QRS peak and measurement of respiratory rate from patient's ECG, we used low-pass filter and impedence method respectively. This system adopt an isolated power for patient's safety. In this system, two output signals can be obtained: R-R interval heart rate) and respiration rate time series. Experimental ranges are 30-240 BPM for ECG and 15-80 BPM for respiration. Results: The system can acquire two signals accurately both in the experimental test using simulator and in real clinical setting. Conclusion: The system developed in this paper is efficient for the acquisition of heart rate and respiration signals. This system will play a role in research area for improving our understanding of the pathophysiologic involvement of the autonomic nervous system in various disease states.