• Korean Journal of Plant Tissue Culture
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• v.25 no.4
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• pp.245-250
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• 1998

Tobacco mosaic virus(TMV) coat protein cDNA was transformed to Nicotiana tabacum cv. NC82 and the transgenic tobacco plants resistant to TMV infection were isolated in the next generation. The expression of TMV coat protein cDNA and genetic stability of the fifth generation of TMV resistant transgenic tobacco plants at the higher temperature were investigated. The TMV coat protein cDNA was amplified by genomic PCR in all the TMV resistant transgenic tobacco plants. The TMV coat protein expressed in the transgenic tobacco plants was detected at very low level by immunoblot hybridization. Even in tansgenic plants that showed the viral symptom only on very late sucker growth (delay type plants), the coat protein expression in the suckers was much less than that of susceptible tobacco infected with TMV. The TMV coat protein expressed in the transgenic tobacco plants was below 0.01% of total protein. Transcription and expression of the coat protein cDNA in delay type plants were observbed at high temperature (38$^{\circ}C$), and TMV replication was suppressed at both 28$^{\circ}C$ and 38$^{\circ}C$. This indicates that unlike the resistance conferred by 'N' gene. TMV resistance of transgenic tobacco plant won't break down at high temperature.

• Korean Journal of Breeding Science
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• v.41 no.4
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• pp.595-598
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• 2009

A new yellow gerbera (Gerbera hybrida Hort.) cultivar "Golden Time" was developed from a cross between '99-ga-2' and '99-na-7-6', followed by seedling and line selections at the Flower Research Institute, Gyeongnam Agricultural Research and Extension Services (ARES) in 2007. Detailed characteristics of the new cultivar were evaluated from 2004 to 2007. "Golden Time" has semi-double type large flowers in a good harmony with yellow (RHS, 17-B) ray floret and brown center. It has good, stable flower shape and strong peduncles, and its vase life was 12.3 days. The average flower yield of "Golden Time" was about 49.5 stems per plant/year in greenhouse trails during 2004 and 2007. This cultivar was registered to the Korea Seed and Variety Service (KSVS) for commercialization in 2009. Year-round production of this cultivar is possible in the greenhouse condition throughout the Korean climate.

• FLOWER RESEARCH JOURNAL
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• v.16 no.2
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• pp.124-127
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• 2008

A new orange-colored, cut flower gerbera (Gerbera hybrida Hort.) cultivar 'Sweety' was developed from a cross between 'Grandeur' and 'Songsongee', followed by selections of seedlings and lines at the Flower Breeding Research Institute, Gyeongnam Agricultural Research and Extension Services (ARES) from 1999 to 2004. Three time evaluations were conducted from 2003 for the detailed characteristics of the new cultivar. 'Sweety' has semi-double type mini- or medium-sized flowers in good harmony with orange(RHS, 38-A) ray florets and a green center. It has good, stable flower shape and strong peduncle, and its vase life was 10.4 days. The average flower yield was about 51.0 stems per plant/year in greenhouse trails during 2003 and 2004. This cultivar was registered to the Korea Seed and Variety Service for commercialization in 2006. Year-round production of this cultivar is possible in the greenhouse condition in Korean climate.

• Korean Journal of Breeding Science
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• v.40 no.3
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• pp.340-343
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• 2008

A new pale yellow gerbera(Gerbera hybrida Hort.) cultivar 'Honey Cream' was developed from a cross between 'Princessa' and 'Picasso', followed by seedling and line selections at the Flower Research Institute, Gyeongnam Agricultural Research and Extension Services(ARES) in 2006. Three times evaluations were conducted from 2003 for the detailed characteristics of the new cultivar. 'Honey Cream' has semi-double type large flowers in good harmony with pale yellow(RHS, 11-D) ray floret and green center. It has good, stable flower shape and strong peduncle, and its vase life was 12.3 days. The average flower yield of 'Honey Cream' was about 48.5 stems per plant/year in greenhouse trails during 2003 and 2006. This cultivar was registered to the Korea Seed and Variety Service(KSVS) for commercialization in 2007. Year-round production of this cultivar is possible in the greenhouse condition in Korean climate.

• Korean Journal of Breeding Science
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• v.40 no.4
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• pp.466-469
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• 2008

A new kidney bean cultivar, "Hwanghyeob 1" was developed at the National Institute of Crop Science in 2005. "Hwanghyeob 1" was selected from a cross between KLG50064 and KLG50073. It has determinate growth habit, white flower, yellow pod color, oval shape of crossed section of pod at the harvesting time for edible pod, white seed coat and middle seed size (21.7 grams per 100 seeds). The average yield of edible pod of "Hwanghyeob 1" was 23.55 M/T per hectare in the yield trials which was carried out at the green house in spring and autumn in 2005. This yield level was 4 percent higher than that of the check cultivar "Kangnangkong 1".

• Korean Journal of Breeding Science
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• v.40 no.1
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• pp.80-83
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• 2008

A new pink gerbera (Gerbera hybrida Hort.) cultivar 'Summer Ring' was developed from a cross between 'Grandeur' and 'Nova Zembla' followed by seedling and line selections at the Flower Breeding Research Institute, Gyeongnam Agricultural Research and Extension Services (ARES) in 2005. Characteristics of the new cultivar were evaluated three times from 2003 to 2004. 'Summer Ring' has semi-double type mini flower in good harmony with pink (RHS, 56-D) ray floret and green center. It has good stable flower shape and strong peduncle, and its vase life was 14.7 days. The average yield of 'Summer Ring' flower was 69 per plant/year in greenhouse yield trails during 2003 and 2004. This cultivar was registered for commercialization in 2005. Commercial production of 'Summer Ring' could be possible throughout the country under greenhouse condition.

• Journal of Bio-Environment Control
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• v.28 no.2
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• pp.158-165
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• 2019

The effects of microbubbles on glucosinolate accumulation and growth of watercress (Nasturtium officinale R. Br.) were investigated. Watercress plant at the 4th mature leaf stage (2 weeks old) were exposed to microbubbles or non-microbubbles generated in an Otsuka-house nutrient solution for 3 weeks in a controlled environment culture room. Stem length of the watercress grown under the microbubbles was 41% shorter than that of the non-microbubbles, showing significantly different. However, shoot fresh and dry weights, root length, leaf length, leaf width, SPAD, and quentum yield of the watercress were not significantly different between treatments. Glucoiberin, glucobrassicin, gluconapin, gluconasturtiin of the watercress grown under microbubbles, excepted for 4-methoxyglucobrassicin, were significantly higher than those of the watercress grown in non-microbubbles. In addition, watercress grown under microbubbles for 3 weeks contained 85% (${\mu}mol/g$ DW) and 65% (${\mu}mol/plant$) more total glucosinolate, respectively. Results indicated that microbubbles generated in a deep flow technique hydroponics system could increase the accumulation of glucosinolate without growth reduction.

• Journal of Bio-Environment Control
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• v.31 no.4
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• pp.319-324
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• 2022

The objective of this study was to determine the proper night interruption of photoperiods and dark periods for accumulating bioactive compounds of Agastache rugosa without decreasing plant growth. Five-week-old seedlings were transplanted in a DFT system with white LEDs. A. rugosa was treated with night interruption time treatments of 18:1:2:3, 18:2:2:2, 18:3:2:1 (light:dark:light:dark), and 20:4 (control) for 4 weeks. There were no significant differences except for leaf length, leaf width, and the number of flowers. The content of antioxidants in the shoot of A. rugosa was high in tilianin and acacetin, and the content of rosmarinic acid (RA) was significantly higher in the underground part. The RA content per dry weight of A. rugosa was 47.92 and 51.46% higher than that of the control in 18:1:2:3 and 18:2:2:2, and tilianin and acactin per dry weight were significantly higher in 18:3:2:1. There was no significant difference in growth due to the same day light integral, but 18:2:2:2 showed high total polyphenol contents. Therefore, it is thought that the effect of increasing secondary metabolites of A. rugosa without degradation of growth can be expected through night interruption treatment in plant factory cultivation systems using artificial light.

• Journal of Bio-Environment Control
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• v.31 no.3
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• pp.188-193
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• 2022

The Korean mint (Agastache rugosa Kuntze) is used as a leaf vegetable. Therefore, it is important to improve the quality and quantity of leaves by maintaining vegetative growth. When the development of a plant is switched from vegetative to reproductive growth, leaf development may be lowered, leading to a decrease in marketability. Therefore, this study was conducted to investigate the effect of the photoperiod on the flowering characteristics and inflorescence development of the Korean mint to select an appropriate photoperiod for leaf production. The seeds were sown in 105-cell trays on 29 November 2021, and planted in each pot on 27 December 2021, when four main leaves appeared. After planting, the light/dark period was adjusted to 10/14hrs, 12/12hrs, 14/10hrs, 16/8hrs. After 19 days of planting, the first flowering occurred in 10/14hrs, which had a short photoperiod, and the 12/12hrs had 20 days for flowering, and other treatments had 21 days. In addition, it was confirmed that the Korean mint has the characteristics of facultative quantitative short-day plant, through the increase in the development of inflorescences as the light period is shorter than the dark period. As for the growth of Korean mint, the longer the photoperiod, the higher values were shown with leaf length, number of leaves, fresh weight, dry weight, and leaf area of the shoot. Therefore, it is considered that 14 h or more light period is suitable for increasing the productivity of Korean mint as a leafy vegetable.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.30 no.1
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• pp.1-6
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• 1985

This experiment was designed to determine the effect of light quality on the appearance of photobleaching leaves during the cure of Burley Tobacco. The harvested and browned tobacco leaves were exposed to sunlight in pipe houses covered with 8 kinds of color vinyls (white, red, black, yellow, purple, orange, blue, green), and exposed to ultraviolet rays(20W x 3) and infrared rays (150W x 2) in curing chamber (1.2 x 1.2 x 1.2m). Photobleaching occured more at lower position leaf and after the leaves being browned when the curing was done in sunlight under a transparent vinyl. But photobleaching leaves were 5-6% of total cured leaves in sunlight under all kinds of color vinyl houses. It seems that photobleaching mainly induced by ultrabiolet rays of sunlight, and humidity too influenced. Yellow, orange and purple vinyl were durable and effective as shading material of color vinyls. since white and red vinyl tore easily in two monthes in strong sunlight and under black and blue vinyl houses curing period was longer than others.