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Studies of cold resistant glycine betaine effect on cold sensitive Bacillus subtilis mutant strains (저온 민감성 바실러스 서브틸리스 돌연변이 균주에서 glycine betaine의 저온 내성에 미치는 영향에 대한 연구)

  • Kim, Do Hyung;Lee, Sang Soo
    • Korean Journal of Microbiology
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    • v.54 no.3
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    • pp.200-207
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    • 2018
  • At high salt concentration, glycine betaine is transported into Bacillus subtilis and growing rate of the cell is not suppressed. Also according to recent studies, cell growth is maintained normal growth rate at low temperature. Low temperature results in a stress response of Bacillus subtilis that is characterized by strong repression of major metabolic activities such as translation machinery and membrane transport. In this regards, genes showing cold sensitive phenotype are cold-induced DEAD box RNA helicases (ydbR, yqfR) and fatty acid desaturases (bkdR, des). Therefore to understand the effect of glycine betaine on cold growth of Bacillus subtilis, we investigated the effect of glycine betaine on growth rate of these deletion mutants showing cold sensitive phenotype. Glycine betaine strongly stimulated growth of wild type Bacillus subtilis JH642 and deletion mutants of ydbR and yqfR at $20^{\circ}C$ (190~686 min $T_d$ difference). On the other hands, glycine betaine does not show growth promoting effects on deletion mutants of bkdR, and des at cold conditions. Same cold protectant growth results were shown with the precursor choline instead of glycine betaine. We investigated the effects of detergents on the cell membrane in bkdR and des deficient strains associated with cell membrane. It was identified that bkdR deficient strain shows retarded growth with detergent such as Triton X-100 or N-lauryl sarcosine compared with wild type cell. Thus, it is possible that deletion mutation of bkdR modifies membrane structure and effects on transport of glycine betaine.

Examining the Debate of Social Security Pension Reform in the United States by 1996 (미국의 사회보장연금 개혁논쟁에 관한 고찰)

  • Won, Seok-Jo
    • Korean Journal of Social Welfare
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    • v.51
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    • pp.5-28
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    • 2002
  • The purpose of this paper is to examine the issues and the characteristics of the recent social security pension debate in the United States. For the purpose the transforming process from the funded system to the pay-as-you-go system in the 1930s, three alternatives of social security reform proposed by the Social Security Administration in 1996, and the other various alternatives proposed by the politicians, the business leaders and the scholars were analysed. While the alternatives were compared, the critical issues could be identified. The core issues were as follows. First, the individual accounts should be newly made or not? Second, who is the main administrator, government or private investment companies? Third, what is important, the inter-generational and the vertical income redistribution effect or the individual equity in social security pension system? Besides, the different positions of the social forces were also examined. The supporters of privatizing the social security pension, supporters of IA and PSA, prefer the value of equity, the effect of promoting savings, the private management of the social security funds, and the investment of the funds to the private capital markets. The supporters of pay-as-you-go system, supporters of MB, prefer the inter-generational and the vertical income redistribution effect of social security pension, and were convinced that fundamental changes of the systems are not necessary, and the insolvency problem could be overcome through a few reform, for example, increasing the rate and decreasing the benefits.

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Detecting Surface Changes Triggered by Recent Volcanic Activities at Kīlauea, Hawai'i, by using the SAR Interferometric Technique: Preliminary Report (SAR 간섭기법을 활용한 하와이 킬라우에아 화산의 2018 분화 활동 관측)

  • Jo, MinJeong;Osmanoglu, Batuhan;Jung, Hyung-Sup
    • Korean Journal of Remote Sensing
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    • v.34 no.6_4
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    • pp.1545-1553
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    • 2018
  • Recent eruptive activity at Kīlauea Volcano started on at the end of April in 2018 showed rapid ground deflation between May and June in 2018. On summit area Halema'uma'u lava lake continued to drop at high speed and Kīlauea's summit continued to deflate. GPS receivers and electronic tiltmeters detected the surface deformation greater than 2 meters. We explored the time-series surface deformation at Kīlauea Volcano, focusing on the early stage of eruptive activity, using multi-temporal COSMO-SkyMed SAR imagery. The observed maximum deformation in line-of-sight (LOS) direction was about -1.5 meter, and it indicates approximately -1.9 meter in subsiding direction by applying incidence angle. The results showed that summit began to deflate just after the event started and most of deformation occurred between early May and the end of June. Moreover, we confirmed that summit's deflation rarely happened since July 2018, which means volcanic activity entered a stable stage. The best-fit magma source model based on time-series surface deformation demonstrated that magma chambers were lying at depths between 2-3 km, and it showed a deepening trend in time. Along with the change of source depth, the center of each magma model moved toward the southwest according to the time. These results have a potential risk of including bias coming from single track observation. Therefore, to complement the initial results, we need to generate precise magma source model based on three-dimensional measurements in further research.

Microstructural Change by Hot Forging Process of Korean Traditional Forged High Tin Bronze (전통기술로 제작된 방짜유기의 열간 단조 과정별 미세조직 변화)

  • Lee, Jae-sung;Jeon, Ik-hwan;Park, Jang-sik
    • Journal of Conservation Science
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    • v.34 no.6
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    • pp.493-502
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    • 2018
  • Currently, the fabrication of a high-tin bronze spoon by traditional manufacturing techniques involves 10 steps in the bronze ware workshop. Hot forging has a major influence on manufacturing and involves two to three steps. The dendritic ${\alpha}$-phase in the microstructure of the high-tin bronze spoon is refined and finely dispersed through hot forging. In addition, twinning is observed in the ${\alpha}$-phase of the hammered part, and the ${\alpha}$-phase microstructure gradually transform from a polygon to a circular shape due to hammering. In this process, the adjacent ${\alpha}$-phases overlap with each other and remain combined after quenching. The microstructure with the overlapping is also observed in bronze artifacts, and this shows the correlation with technical system. The results of the experimental hot forging of Cu-22%Sn alloys show that the decrease in in the amount of the dendritic microstructure, which forms during casting, is in proportion to the number of processing steps and that the refined grain obtained by hammering contributes to the improvement in the strength of the material. From the hammering marks, which are observed on both the bronze artifact excavated from archaeological sites and on the high-tin bronze spoon produced in the traditional workshop, it is presumed that the knowledge regarding the unrecorded manufacturing system of bronze ware in ancient times has been passed down in a traditional way up to the system used currently.

Design of Knowledge-based Spatial Querying System Using Labeled Property Graph and GraphQL (속성 그래프 및 GraphQL을 활용한 지식기반 공간 쿼리 시스템 설계)

  • Jang, Hanme;Kim, Dong Hyeon;Yu, Kiyun
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.40 no.5
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    • pp.429-437
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    • 2022
  • Recently, the demand for a QA (Question Answering) system for human-machine communication has increased. Among the QA systems, a closed domain QA system that can handle spatial-related questions is called GeoQA. In this study, a new type of graph database, LPG (Labeled Property Graph) was used to overcome the limitations of the RDF (Resource Description Framework) based database, which was mainly used in the GeoQA field. In addition, GraphQL (Graph Query Language), an API-type query language, is introduced to address the fact that the LPG query language is not standardized and the GeoQA system may depend on specific products. In this study, database was built so that answers could be retrieved when spatial-related questions were entered. Each data was obtained from the national spatial information portal and local data open service. The spatial relationships between each spatial objects were calculated in advance and stored in edge form. The user's questions were first converted to GraphQL through FOL (First Order Logic) format and delivered to the database through the GraphQL server. The LPG used in the experiment is Neo4j, the graph database that currently has the highest market share, and some of the built-in functions and QGIS were used for spatial calculations. As a result of building the system, it was confirmed that the user's question could be transformed, processed through the Apollo GraphQL server, and an appropriate answer could be obtained from the database.

A Study on Effective Adversarial Attack Creation for Robustness Improvement of AI Models (AI 모델의 Robustness 향상을 위한 효율적인 Adversarial Attack 생성 방안 연구)

  • Si-on Jeong;Tae-hyun Han;Seung-bum Lim;Tae-jin Lee
    • Journal of Internet Computing and Services
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    • v.24 no.4
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    • pp.25-36
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    • 2023
  • Today, as AI (Artificial Intelligence) technology is introduced in various fields, including security, the development of technology is accelerating. However, with the development of AI technology, attack techniques that cleverly bypass malicious behavior detection are also developing. In the classification process of AI models, an Adversarial attack has emerged that induces misclassification and a decrease in reliability through fine adjustment of input values. The attacks that will appear in the future are not new attacks created by an attacker but rather a method of avoiding the detection system by slightly modifying existing attacks, such as Adversarial attacks. Developing a robust model that can respond to these malware variants is necessary. In this paper, we propose two methods of generating Adversarial attacks as efficient Adversarial attack generation techniques for improving Robustness in AI models. The proposed technique is the XAI-based attack technique using the XAI technique and the Reference based attack through the model's decision boundary search. After that, a classification model was constructed through a malicious code dataset to compare performance with the PGD attack, one of the existing Adversarial attacks. In terms of generation speed, XAI-based attack, and reference-based attack take 0.35 seconds and 0.47 seconds, respectively, compared to the existing PGD attack, which takes 20 minutes, showing a very high speed, especially in the case of reference-based attack, 97.7%, which is higher than the existing PGD attack's generation rate of 75.5%. Therefore, the proposed technique enables more efficient Adversarial attacks and is expected to contribute to research to build a robust AI model in the future.

Research on Artistic Artmask for Lifecaretainment (라이프케어테인먼트를 위한 예술적 아트마스크 연구)

  • Yoon, Hee
    • Journal of Korea Entertainment Industry Association
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    • v.13 no.3
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    • pp.269-278
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    • 2019
  • This study is intended to present the direction of art mask design development and help people's life care by producing an art mask with the motif of the characteristic of abjection concept. It went through the definition and characteristics of abject and abjection focusing on previous research, professional books and art works. The characteristics of abjection based on theoretical research are to express death with dead bodies and skulls, including the dismantling of ideal beauty standards, the expression of fragmented bodies, and the expression of sick and alienated bodies. This researcher produced 6 research works by combining the fascinating abjection, induced by ugly and disgusting abject, to design factors. These works fragmented each part of the facial form by the cutting and dismantling, and made viewers feel the abjection by giving a grotesque deformation. The results obtained through the research works are as follows. First, It was found that the abjection characteristics-applied works was helpful in the treatment of the mental wound of modern people. Second, the production of works based on the abjection characteristics paved the way to expand the scope of art mask design concepts and establish the new design ideas. Third, the application of the motif using the abjection characteristics to the art mask design showed that the use of various objects could express creative designs and secure diversity in using materials. In the future, this researcher hopes that such research will be used as the basic data of the follow-up study on art mask design, and it will help develop art mask designs.

Implementation of Semi-infinite Boundary Condition for Dynamic Finite Element Analysis (동적 유한요소해석에서의 반무한 경계조건의 실행)

  • Choi, Chang-Ho;Chung, Ha-Ik
    • Journal of the Korean Geotechnical Society
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    • v.22 no.9
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    • pp.37-43
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    • 2006
  • Dynamic numerical analysis of geotechnical problems requires a way to simulate the decrease of energy as the domain of interest gets larger. This phenomenon is usually referred to as radiation damping or geometric attenuation and it is distinguished from material damping in which elastic energy is actually dissipated by viscous, hysteretic, or other mechanism. The fact that the domain of analysis in numerical modeling must be chosen, however, causes a need for special attention at the boundary. This observation leads directly to the idea of determining the dynamic response of the interior region from a finite model consisting of the interior region subjected to a boundary condition which ensures that all energy arriving at the boundary is absorbed. This paper presents a simple methodology to simulate transmitting boundaries condition using viscoelastic infinite elements within the recently developed "OpenSees" finite element code. The methodology used here provides that the level of absorption for traveling waves is efficient enough for practical purposes, but unsatisfactory for the case of sharp incident angles. The effectiveness of the infinite elements for the absorption of incident waves at boundaries is evaluated via example analysis.

Experimental Study on Flexural Behavior of RC Beams Strengthened with Prestressed CFRP Plate (CFRP판으로 프리스트레싱 보강한 RC 보의 휨거동에 관한 실험적 연구)

  • Han, Sang-Hoon;Hong, Ki-Nam;Kim, Hyung-Jin;Woo, Sang-Kyun
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.26 no.2A
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    • pp.301-310
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    • 2006
  • Carbon fiber reinforced polymer (CRFP) materials are well suited to the rehabilitation of civil engineering structures due to their corrosion resistance, high strength to weight ratio and high stiffness to weight ratio. Their application in the field of the rehabilitation of concrete structures is increased due to the vast number of bridges and buildings in need of strengthening. However, RC members, strengthened with externally bonded CFRP plates, happened to collapse before reaching the expected design failure load. Therefore, it is necessary to develop the new strengthening method to overcome the problems of previous bonded strengthening method. This problems can be solved by prestressing the CFRP plate before bonding to the concrete. In this study, a total of 21 specimens of 3.3 m length were tested by the four point bending method after strengthening them with externally bonded CFRP plates. The CFRP plates were bonded without prestress and with various prestress levels ranging from 0.4% to 0.8% of CFRP plate strain. All specimen with end anchorage failed by a plate fracture regardless of the prestress levels while the specimen without end anchorage failed by the separation of the plate from the beam due to premature debonding. The cracking loads was proportionally related to the prestress levels, but the maximum loads of specimens strengthened with prestressed CFRP plates were insignificantly affected by the prestress levels.

Preliminary Structural Geometry Interpretation of the Pyeongchang Area in the Northwestern Taebaeksan Zone, Okcheon Belt: A Klippe Model (옥천대 북서부 태백산지역 평창 일대의 클리페 모델 기반 구조기하 형태 해석 예비 연구)

  • Heunggi Lee;Yirang Jang;Sanghoon Kwon
    • Economic and Environmental Geology
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    • v.56 no.6
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    • pp.831-846
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    • 2023
  • The Jucheon-Pyeongchang area in the northwestern Taebaeksan Zone of the Okcheon fold-thrust belt preserved several thrust faults placing the Precambrian basement granite gneisses of the Gyeonggi Massif on top of the Early Paleozoic Joseon Supergroup and the age-unknown Bangrim Group. Especially, the thrust faults in the study area show the closed-loop patterns on the map view, showing older allochthonous strata surrounded by younger autochthonous or para-autochthonous strata. These basement-involved thrusts including Klippes will provide important information on the hinterland portion of the fold-thrust belt. For defining Klippe geometry in the thrust fault terrains of the Jucheon-Pyeongchang area by older on younger relationship, the stratigraphic position of the age-unknown Bangrim Group should be determined. The Middle Cambrian maximum depositional age by the detrital zircon SHRIMP U-Pb method from this study, together with field relations and previous research results suggest that the Bangrim Group overlies the Precambrian basement rocks by nonconformity and underlies the Cambrian Yangdeok Group (Jangsan and Myobong formations). The structural geometric interpretation of the Pyeongchang area based on newly defined stratigraphy indicates that the Wungyori and Barngrim thrusts are the same folded thrust, and can be interpreted as a Klippe, having Precambrian hanging wall granite gneisses surrounded by younger Cambrian strata of the Joseon Supergroup and the Bangrim Group. Further detailed structural studies on the Jucheon-Pyeongchang area can give crucial insights into the basement-involved deformation during the structural evolution of the Okcheon Belt.