• KSBB Journal
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• v.21 no.4
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• pp.306-311
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• 2006

Cell preservation is indispensable in animal cell culture process and should be established according to the cell characteristics. In this study, we experimented hypothermic preservation of CHO cells that is widely used in pharmaceutical industry to produce therapeutic proteins and established a stable method of preservation. The highest viability of CHO cells was obtained when the cells were preserved using rolling tube, which means the cells should be suspended to avoid the cell lumping during the preservation. Also, we obtained superior preservation result under the anaerobic condition. To evaluate the serum-free media as a preservation solution, we investigated cell growth after hypothermic preservation using serum-free media. High cell viability and normal cell growth was observed during 10 days using serum-free media. Moreover, we found that more effective preservation when ${\alpha}$-tocopherol and retinoic acid is added to media as an additive. In the case of 1 liter large scale hypothermic preservation using established protocol, cell viability and growth rate was obtained as good as small scale one. This study is considered to be helpful for hypothermic preservation of CHO cells and large scale hypothermic preservation may be available through the further studies.

• Korean Journal of Heritage: History & Science
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• v.43 no.3
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• pp.26-47
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• 2010

When the term conservation is used with regard to excavated features, it means not only conservation but also restoration. Restoring the features here does not imply restoring their original form but restoring their form at the moment of excavation. That means, the conservation of excavated features includes the concept of both reparation and restoration. The way of conserving excavated features can be largely categorized into on-site conservation and transfer conservation. On-site conservation means to conserve excavated features as they were at the excavation site. It can be further categorized into soil-covered on-site conservation, in which excavated features are covered with soil to prevent them from being damaged, and exposed on-site conservation in which the features were conserved as they were exposed. Transfer conservation is operated on the premise that excavated features are transferred to another place. It can be further categorized into original form transfer, transcribing transfer, reproduction transfer, and dismantlement transfer. Original form transfer refers to the method of moving the original forms of excavated features to another place. Transcribing transfer refers to moving some of the surfaces of excavated features to another place. Reproduction transfer refers to restoring the forms of excavated features in another place after copying the forms of excavated features at the excavation site. Dismantlement transfer refers to the method of restoring excavated features in a place other than the excavation site in the reverse order of dismantlement after dismantling the features at the excavation site. The most fundamental issue regarding conserving excavated features is the conservation of their original forms. However, the conservation of excavated features tends to be decided depending on a variety of conditions such as society, economy, culture, and local situations. In order to conserve excavated features more effectively, more detailed and specialized conservation methods should be created. Furthermore, continuing research is needed to find the most effective way of conserving them through exchange with other neighboring academic fields and scientific technology.

• Maxillofacial Plastic and Reconstructive Surgery
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• v.15 no.4
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• pp.253-268
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• 1993

The author studied on the 109 cases of ameloblastoma which had been diagnosed with biopsy during the period of 1962 to 1992 at the Kyungpook National University Hospital, Kyemyung University Hospital, Youngnam University Hospital, Catholic Medical School Hospital, Pusan National University and Maryknoll Hospital. This study contains the statistical analysis of the treatment method according to the clinicopathological findings such as sex, age, location, chief complaints, duration, radiographic findings, size and recurrence. The results were as follows : 1. The incidence rate was 59.7% in male and 40.3% in female. 2. At the time of diagnosis, the age of the patients ranged from 9 to 69 years(average 31.4years). The cases of 72.4% were in the 2nd, 3rd, and 4th decades of life. 3. The majority of cases occurred in the mandible 88.9%, especially in the mandiblar angle area 57.8%. 4. The most prevalent chief complaints was swelling 58.7%. 5. As regards duration, the cases less than 1 year appeared 49.5% and average duration is 31.34months. 6. Unilocular type showed 43.1% and multilocular type appeared 56.9%. 7. Conservative treatment was performed 39.4% and radical treatment was employed 60.6%. 8. There were variations of lesional sizes between 2.0cm and 15cm(average 6.26cm). 9. The recurrence rate is 29.4%. 10. The frequency of treatment was not in association with sexes, locations, chief complaints and sizes. 11. Radical treatment was performed more frequently in cases of older age group(above 20 years old), multilocular cases, above average duration(31.34 months) group and recurrent cases (P<0.05).

• 한국문화재보존과학회:학술대회논문집
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• 2004.03a
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• pp.63-63
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• 2004

• Journal of the Korean Arthroscopy Society
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• v.13 no.2
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• pp.87-96
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• 2009

최근 전방십자인대 손상의 치료에 남아있는 인대 다발이 이식건의 생물학적 치유를 도우며 고유 수용감각(proprioception) 기능을 유지할 뿐만 아니라 슬관절의 전방 전위 억제의 생역학적 기능을 가지는 것으로 연구되고 있다. 이러한 이론을 바탕으로 남아있는 전방십자인대를 보존하는 노력들은 다양하게 시도되고 있다. 전방십자인대 손상의 자연 경과는 아직도 논란이 되고 있으나 선택적인 환자에 있어서 보존적 치료는 비교적 좋은 결과를 얻을 수 있으므로 불필요한 재건술을 줄일 수 있다. 최근 시도되고 있는 선택적 다발 재건술 및 남아있는 다발을 보존하는 전방십자인대 재건 수술 수기들은 다양한 방법으로 시도되고 있으며 남아있는 다발을 보존하는 장점이 있으나 협소한 적응증을 가진다. 저자들의 남아있는 다발을 보존하는 전방십자인대 재건술은 자가 슬괵건을 이용한 단일다발 재건술을 시행하면서 잔존하는 이완된 다발에 봉합사를 통과시킨 후 봉합사를 추가적인 터널 없이 bioabsorbable cross pin (RIGIDfix system, Mitek, Johnson & Johnson, USA) 구멍을 통하여 당기고 고정하는 방법이다. 전방십자인대 재건술시 남아있는 다발을 보존하는 방법은 이식건의 생물학적 치유을 촉진하고 또한 경골 부착부에 남아있는 기계수용체를 보존하므로 기능적 회복에 도움이 될 것으로 기대된다. 저자들의 방법은 비교적 광범위한 적응증을 가지며 적은 합병증으로 남아있는 다발을 효과적으로 보존할 수 있는 술식으로 전방십자인대 수술시 좋은 치료 선택의 하나로 사료된다.

• Journal of Conservation Science
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• v.26 no.3
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• pp.341-348
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• 2010

After excavation work of relics, historical site which has historically meaningful, are preserved using the various methods. There are three method in the relocation methods that are original relocation method, the remaining structure-peel method, and soil peel method. The original relocation method is restored after relocating in historical site such as residential site, iron foundry site, kiln site, old mound. The remaining structure-peel method are restored only the feature of exposed remaining structure using polymeric resin, when it is difficult to relocate the entire remaining structure. And soil-peel method is exhibited after peeling in case when soil layers such as grave of old mound, foundation of building site, sedimentary deposit layer, shell heap, and etc. Soil-peel method becomes important historical data of changes according to environment at that time, that is, flooding by storm, traces of fire and living features of that time such as heaps of shells discarded after eating shellfish and living wastes. In particular, in case of soil layer for preparing foundation sites of building by compacting in turn soils with different components such as clay soil, rough sand soil and etc, it becomes important data which can judge foundation technology of that time. It can be said to be an important data preservation method for utilizing these historical data as historical data as well as for the purpose of education, exhibitions and public relations which can be shared not only by experts but also by general public. In this paper, we present the reliable definition of soil-peel method in various preservation methods and explain the using polymer in this method. So, we will come up with the accurate index about this method that is used the eminent analysis method for soil layer.

• Journal of Conservation Science
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• v.6 no.2 s.8
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• pp.149-157
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• 1997

Based on results reported in the literature, the conservation of outdoor wood, dry wood, and waterlogged wood which contained archaeological and cultural values were reviewed in a chemical point of view.

• Proceedings of the Korea Information Processing Society Conference
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• 2002.11a
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• pp.699-702
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• 2002

본 논문에서는 anisotropic diffusion 방법의 일종인 평균곡률 확산 (Mean Curvature Diffusion) 방법을 이용하여 영상에 포함된 잡음은 제거하고 동시에 에지는 보존하는 기법을 제안한다. 평균곡률 확산은 2 차원 영상의 밝기값을 3 차원 공간상의 z 좌표에 대응시켜 영상의 밝기값에 대응하는 공간 상의 곡면을 구성하고 이 곡면을 평균곡률에 비례하는 속도로 확산시킨다. 확산이 진행되면서 평균곡률이 영이 되는 에지에서는 확산이 발생하지 않고 잡음 등의 영향이 많은 에지 이외의 영역에서는 확산이 빠른 속도로 진행된다. 기존의 평균곡률 확산 방법의 성능을 개선하기 위해 최소/최대 흐름 방법을 평균곡률 확산 방법과 결합시키고 영상의 2 차 도함수를 사용하여 d얇은 에지를 보존하였다. 실험을 통해 제안한 방법이 기존의 방법보다 잡음 제거와 에지 보존 성능이 우수함을 확인할 수 있었다.

• Journal of Conservation Science
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• v.12 no.1 s.15
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• pp.71-82
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• 2003

The paper relics excavated from the 4th excavation of the historical spot No.259 Seonwon temple area were analyzed and conserved. Because these works remained folded, the most important conservation treatment was a separation. This process depended on the state of the works: dry state works were separated using Gore-Tex chamber and the filtrated water, wet state works were separated through vacuum freeze drying method. Analyses of the paper relics performed were examination on the paper to find its distinction, binocular microscope and SEM on fiber, and XRF on character ingredient. This study presumed the times though $C^{14}$ dating of these works. The works were able to be classified through analysis and conservation treatment. This separation made lost part of the works conserve an archetype. This study presented the application of conservation methods on the paper relics which are in the bad condition and obtained information through carbon dating of it.

• Proceedings of the Korean Information Science Society Conference
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• 2011.06a
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• pp.61-64
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• 2011

디지털화의 눈부신 발전으로 인해 대부분의 기록물들은 종이 위주의 생산방식에서 전자문서방식으로 패러다임이 변하고 있다. 이에 따라 정부적인 차원에서 이러한 전자문서에 대한 장기보존에 대한 연구가 요구되고 있으며, 이미 많은 국가에서 표준을 정의하여 기록물에 대한 장기보존 기법에 대한 연구가 활발하게 진행되고 있다. 이들 시스템의 대부분은 IS014721 표준으로 지정된 디지털 객체의 장기보존을 위한 참조모델인 OAIS(Open Archival Information System) 정보패키지 개념으로 사용한다. 이 정보패키지 방법은 보존하고자 하는 콘텐츠에 메타데이터를 두어 콘텐츠와 함께 인캡슐레이션을 함으로써 보존 및 활용을 가능하게 해준다. 그러나 기존에 연구는 장기보존에 관한 시스템 설계가 대부분이었고, 실제로 패키지를 구성하고 저장하는 방법에 대한 연구는 미진한 실정이었다. 따라서 본 논문에서는 멀티미디어 기록물을 위한 장기 보존 패키지를 설계 및 적용 하고자 한다. 이를 위하여 OAIS 참조모델을 기반으로 장기 보존을 위한 대용량 비디오 관리 시스템의 보존 정보 패키지의 구조를 제안 하였다. 장기보존 패키지는 기록물 그 자체와 기록물을 보존하기 위한 정보, 기록물을 설명하기 위한 정보를 저장한다. 또한, 추후 활용과 배포를 위하여 기록물 패키지의 정보를 저장하는 Meta-AIP와 실제로 기록물을 저장하는 Real-AIP로 구성되어 있다. 또한, METS를 이용한 실제 패키지의 예를 보임으로써 비디오 기록물 관리시스템에 적용 가능 함을 보였다.