• Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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• 1994.07a
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• pp.7-8
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• 1994

한국산 표고버섯 균사체를 액체배양하여 천연항암물질로 알려진 단백다당체를 추출한 후 그 물질의 특성에 대하여 검토하였다. 균사체의 최적 재양조건을 TGY배지로 조사한 바 온도 $25^{\circ}C$, 배양초기 pH4.0, 교반속도 300rpm, 균사배지 접종량을 10.0%로 하고 산소 통기량을 1.0volume of ait/volume of medium/mimute으로 하였을때 가장 양호한 조건이였으며, 대량생산 하기 위한 SCM배지에서 최적의 C/N비는 13.1오써 7일간 배양하였을때 18.8g/L의 균사령을 얻었으며, 이때 생산수율은 0.46으로 나타났다. 발효가 끝난 배양액에서 균사, 여액 그리고 배양액의 전체에서 단백다당체를 분리한 결과 각 분획에서 단백다당체가 각각 0.55%, 0.12%, 0.69%가 회수되어 배양액 전체에서 단백다당체를 추출하는 것이 바람직하며, 추출방법으로 열수추출, glass bead추출 및 cellulasa처리를 하여 단백다당체의 수율을 비교한 결과 0.25-0.5mm glass nead로 30분간 균사체를 분쇄한 다음 열수추출을 1시간을 하였을때 990mg/100ml의 단백다당체를 얻을 수 있었다. 고단백다당체를 1차 단백질 가수분해 효소로 분해하고, EDAE cellulose 및 Sepadex G-100 column chromatography로 정제한 후, TLC/FLD, ultracentrifugation한 결과 순수한 물질임을 알 수 있었다. 단백다당체의 항암효과 조사중 in vitro배양에서 $P_{388}$과 $L_{1210}$에 대한 단백다당체의 활성단위 1 unit는 1mg정도였으며, 인체의 장암세포인 HCT-48, HRT-18, HT-29 밀 간암세포인 Hep G2 대한 생육저해 단위는 각각 4.4, 3.6, 6.6, 2.6mg이었다. HCT-48과 Hep G2 세포의 크기 분포도는 대조군에 비하여 시간이 경과함에 따라, 그리고 단백다당체의 농도가 증가함에 따라 peak가 작은 size 쪽으로 이동하였다. 또, 단백다당체를 첨가 배양한 HCT-48과 Hep G2세포의 현미경 관찰에서 본래의 암세포 형태가 변형되고 크기가 감소하며 세포사이의 경계막이 흐트러지면서 세포수가 감소하고 사멸하였다. In vivo실험에서는 대조군보다 단백다당체를 첨가한 군에서 항체 형성능력이 대조군에 비하여 형질세포가 2배로 증가하였다. 단백다당체의 화학적 성분 분석에서 다당함량은 46.1%이면 구성다당류는 glucose, galactose, mannose, xylose로 구성되었고 단백질의 함량은 7.28%이며, 구성아미노산은 15종의 아미노산으로 되었다. 또 무기물은 Na, K, Zn, Ca등의 순으로 이루어 짐을 알 수 있었다.

• Nuclear Engineering and Technology
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• v.27 no.3
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• pp.344-357
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• 1995

The gap conductance between the fuel and the sheath depends strongly on the gap width and has a significant influence on the amount of initial stored energy. The modified Ross and Stoute gap conductance model in ELESTRES is based on a simplified thermal deformation model for steady-state fuel temperature calculations. A review on a series of experiments reveals that fuel pellets crack relocate, and are eccentrically positioned within the sheath rather than solid concentric cylinders. In this paper, the hue recently-proposed gap conductance models (offset gap model and relocated gap model) are described and are applied to calculate the fuel-sheath gap conductances under experimental conditions and normal operating conditions in CANDU reactors. The good agreement between the experimentally-inferred and calculated gap conductance values demonstrates that the modified Ross and Stoute model was implemented correctly in ELESTRES. The predictions of the modified Ross and Stoute model provide conservative values for gap heat transfer and fuel surface temperature compared to the offset gap and relocated gap models for a limiting power envelope.

• Journal of the Earthquake Engineering Society of Korea
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• v.7 no.2
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• pp.67-73
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• 2003

The purpose of this study is to evaluate seismic performance of reinforced concrete bridge columns under varying axial force. A computer program, named RCAHEST(reinforced concrete analysis in higher evaluation system technology), for the analysis for reinforced concrete structures was used. Material nonlinearity is taken into account by comprising tensile, compressive and shear models of cracked concrete and a model of reinforcing steel. The smeared crack approach is incorporated. In boundary plane at which each member with different thickness is connected, local discontinuity in deformation due to the abrupt change in their stiffness can be taken into account by introducing interface element. The effect of number of load reversals with the same displacement amplitude has been also taken into account to model the reinforcing steel and concrete. The proposed numerical method for seismic performance evaluation of reinforced concrete bridge columns under varying axial force is verified by comparison with reliable experimental results.

• Journal of the Korean Institute of Telematics and Electronics S
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• v.36S no.1
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• pp.104-114
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• 1999

This paper proposes an efficient method for the automatic generation of personalized 3D face model from color image sequence. To detect a robust facial region in a complex background, moving color detection technique based on he facial color distribution has been suggested. Color distribution and edge position information in the detected face region are used to extract the exact 31 facial feature points of the facial description parameter(FDP) proposed by MPEG-4 SNHC(Synthetic-Natural Hybrid Coding) adhoc group. Extracted feature points are then applied to the corresponding vertex points of the 3D generic face model composed of 1038 triangular mesh points. The personalized 3D face model can be generated automatically in less then 2 seconds on Pentium PC.

• Journal of the Korea Concrete Institute
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• v.17 no.6 s.90
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• pp.983-992
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• 2005

The shear failure modes of fiber reinforced polymer(FRP) strengthened concrete beams are quite different to those of the beams strengthened with steel stirrups. When the beams are strengthened with larger amount of FRP composites, the beams normally fail in shear due to concrete crushing before the FRP reaches its rupture strain. In order to predict the shear strength of such beams, the actual rupture strain must be known. The equations previously reported in the technical literature adopt an effective reduction factor for the rupture strain. These equations may not be applicable to FRP strengthened RC beams that are beyond the experimental application limits, because most of these equations are empirical in nature. This paper presents the results of an analytical study on the performance of reinforced concrete beams externally wrapped with FRP composites and internally reinforced with conventional steel stirrups.

• Journal of the Korea Concrete Institute
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• v.14 no.4
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• pp.467-473
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• 2002

The purpose of this study is to investigate the inelastic behavior and ductility capacity of reinforced concrete frame subjected to cyclic lateral load and to provide result for developing improved seismic design criteria. A computer program named RCAHEST(Reinforced Concrete Analysis in Higher Evaluation System Technology) for the analysis of reinforced concrete structures was used. Material nonlinearity is taken into account by comprising tensile, compressive and shear models of cracked concrete and a model of reinforcing steel. The smeared crack approach is incorporated. The strength increase of concrete due to the lateral confining reinforcement has been taken into account to model the confined concrete. In boundary plane at which each member with different thickness is connected local discontinuous deformation due to the abrupt change in their stiffness can be taken into account by introducing interface element. The effect of number of load reversals with the same displacement amplitude has been also taken into account to model the reinforcing steel. The proposed numerical method for the inelastic behavior and ductility capacity of reinforced concrete frame subjected to cyclic lateral load is verified by comparison with reliable experimental results.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.15 no.3
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• pp.399-407
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• 2002

A three-dimensional method of analysis is presented for determining the free vibration frequencies and mode shapes of hollow bodies of revolution (i.e., thick shells), not limited to straight line generators or constant thickness. The middle surface of the shell may have arbitrary curvatures, and the wall thickness may vary arbitrarily. Displacement components$U_\Phi, U_z, U_\theta$ in the meridional, normal and circumferential directions, respectively, are taken to be sinusoidal in time, periodic in$\theta$, and algebraic polynomials in the$\Phi$and z directions. Potential(strain) and kinetic energies of the entire body are formulated, and upper bound values of the frequencies are obtained by minimizing the frequencies. As the degrees of the polynomials are increased, frequencies converge to the exact values. Novel numerical results are presented for two types of thick conical shells and thick spherical shell segments having linear thickness variations. Convergence to four digit exactitude is demonstrated for the first five frequencies of both types of shells. The method is applicable to thin shells, as well as thick and very thick ones.

• Journal of the Korean earth science society
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• v.33 no.6
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• pp.534-543
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• 2012

The relationship between tectonic uplift and geophysical analysis of gravity anomalies and the in-situ stress fields in the Otway Ranges, SE Australia is addressed in this study to understand the nature and possible mechanism for the neotectonic movements. The uplift axis of the ranges is coincident with the regional Bouguer gravity highs whereas thick Tertiary sedimentary successions are highly correlated with the gravity lows along the basin rift geometry. This result suggests that the gravity highs are separated by the thick Tertiary sedimentary successions. Regional structural trends associated with faults and foldings of the deformed surfaces are consistent with the prevailing NW-SE $S_{Hmax}$ trend in this part of the continent. The anomalously positive correlation between topography and Bouguer gravity fields suggests possibly a lithospheric flexural deformation mode at a long wavelength (order of $10^2$ kms) in the region. It also suggests that the reactivation of pre-existing lithospheric structures driven by plate boundary forces plays a key role in this mode.

• Journal of the Korean Institute of Gas
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• v.22 no.1
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• pp.60-63
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• 2018

This paper presents the FEM analysis results on the strength safety of a gas valve for a LPG cylinder. Based on the FEM analysis, the maximum von Mises stress on the boundary zone between a safety valve and the upper area of the thread is 99.2 MPa for the supplied gas pressure of 3.5 MPa in which the gas valve is fully opened. The maximum von Mises stress of 99.2 MPa is considered as safety value, because that value is lower than the yield stress of a brass material. In this case, the maximum deformation at the upper right part of the pressure regulator is 0.002mm. The maximum deformation zone is not a meaning part of the sealing part such as an O-ring or a diaphragm of a gas valve and a pressure regulator. The proposed hybrid gas valve model in which is integrated with a conventional cut-off valve and a pressure regulator is recommended as a gas leakage free mechanism and minimized compact size for a LPG cylinder.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.23 no.3
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• pp.248-257
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• 2011

In the present study, we proposed a new numerical wave tank model to analyze the vertical tension-leg circular floating bodies, using a 2-D Navier-Stokes solver. An IBM(Immersed Boundary Method) capable of handling interactions between waves and moving structures with complex geometry on a standard regular Cartesian grid system is coupled to the VOF(Volume of Fluid) method for tracking the free surface. Present numerical results for the motions of the floating body were compared with existing experimental data as well as numerical results based on FAVOR(Fractional Area Volume Obstacle Representation) algorithm. For detailed examinations of the present model, the additional hydraulic experiments for floating motions and free surface transformations were conducted. Further, the versatility of the proposed numerical model was verified via the numerical and physical experiments for the general rectangular floating bodies. Numerical results were compared with experiments and good agreement was archived.