• Title/Summary/Keyword: 변이음

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Studies on Molecular Plasticity of Bergmann Glia following Purkinje Cell Degeneration (조롱박신경세포의 변성에 따른 버그만아교세포의 면역조직학적 연구)

  • Yoon, Chul-Jong;Cho, Sa-Sun;Lee, Ha-Kyu;Park, Min-Chul
    • Applied Microscopy
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    • v.35 no.3
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    • pp.165-176
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    • 2005
  • Studies on molecular plasticity of Bermann glia (BG) after harmaline-induced Purkinje cell (PC) degeneration in the rat cerebellum. The intimate structural relationship between BG and PC, evidenced by the sheathing of the PC dendrites by veil-like process from the BG has been suggestive of the close functional relationship between these two cell types. However, little is known about metabolic couplings between these cells. This study designed to investigate molecular plasticity of BG in the rat cerebellum in which PCs were chemically ablated by harmaline treatment. Immunohistochemical examination reveals that harmaline induced PC degeneration causes a marked glial reaction in the cerebellum with activated BG and microglia aligned in parasagittal stripes within the vermis. In these strips, activated BG were associated with upregulaion of metallotheionein, while GLAST and was down regulated, as compared with nearby intact area where both BG are in contact with PCs. The data from this study demonstrate that BG can change their phenotypic expression when BG loose their contact with PCs. It is conceivable that activated BG may upregulate structural proteins, metallothionein expression to use for their proliferation and hypertrophy; metallothionein expression to cope with oxidative stress induced by PC degeneration and microglial activation. On the contrary, BG may down regulated expression of GLAST because sustained loss of contact with PCs would eliminate the necessity for the cellular machinery involved glutamate metabolism. In conclusion, BG might respond man to death of PCs by undergoing a change in metabolic state. It seems possible that signaling molecules released from PCs regulates the phenotype expression of BG. Also ultrastructures in the organelles of normal PC and BG are distinguished by mitochondrial appearance, and distributed vesicles at the synaptic area in the cytoplasm.

Ultrastructure of Degenerating Axon Terminals in the Basal Forebrain Nuclei of the Rat following Prefrontal Decortication (이마앞겉질을 제거시킨 흰쥐 앞뇌의 바닥핵무리에서 변성축삭종말의 미세구조연구)

  • Ahn, Byung-June;Ko, Jeong-Sik;Ahn, E-Tay
    • Applied Microscopy
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    • v.35 no.3
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    • pp.135-152
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    • 2005
  • Prefrontal cortex is a psychological and metaphysical cortex, which deals with feeling, memory, planning, attention, personality, etc. And it also integrates above-mentioned events with motor control and locomotor activities. Prefrontal cortex works as a highest CNS center, since the above mentioned functions are very important for one's successful life, and further more they are upgraded every moments through memory and learning. Many of these highest functions are supposed to be generated via forebrain basal nuclei (caudate nucleus, fundus striati nucleus, accumbens septi nucleus, septal nucleus, etc.). In this experiment, prefrontal efferent terminals within basal forebrain nuclei were ultrastructurally studied. Spraque Dawley rats, weighing $250{\sim}300g$ each, were anesthetized and their heads were fixed on the stereotaxic apparatus (experimental model, David Kopf Co.). Rats were incised their scalp, perforated a 3mm-wide hole on the right side of skull at the 11mm anterior point from the frontal O point (Ref. 13, Fig. 1), suctioned out the prefrontal cortex including cortex of the frontal pole, with suction instrument. Two days following the operations, small tissue blocks of basal forebrain nuclei were punched out, fixed in 1% glutaraldehyde-1% paraformaldehyde solution followed by 2% osmium tetroxide solutions. Ultrathin sections were stained with 1% borax-toluidin blue solution, and the stained sections were obserbed with an electron microscope. Degenerating axon terminals were found within all the basal forbrain nuclei. Numbers of degenerated terminals were largest in the caudate nucleus, next in order, in the fundus striati nucleus, in the accumbens septi nucleus, and the least in the septal nucleus. Only axospinous terminals were degenerated within the caudate nucleus and the fundus striati nucleus, and they showed the characters of striatal motor control system. Axodendritic and axospinous terminals were degenerated within the accumbens septi nucleus and the lateral septal nucleus, and they showed the characters of visceral limbic system. Prefrontal role in integrating the limbic system with the striatal system, en route basal forebrain nuclei, was discussed.

Differences Between Wearing Styles and Preferring Styles and the Sensibility According to Men's Fashion Style (남성복의 감성 및 선호 스타일과 실제 착용간의 차이)

  • Rim, Byungmook;Lee, Janghyung;Kim, Jisu;Na, Youngjoo
    • Science of Emotion and Sensibility
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    • v.19 no.4
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    • pp.71-82
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    • 2016
  • As times change rapidly the lifestyle, personality, and values of men have changed diversely. Not only have preferences for men's clothing changed, men's fashion market has also grown, and novel, non-preexisting styles have come into place. Also, there are many studies on sensibility of women's fashion while studies on sensibility of men's fashion are insufficient. This study categorized common styles for men in their 20s into 7 different representative samples and investigated consumers' sensibility evaluations for each representative sample. Style 1 (suit), style 2 (rider jacket + skinny pants), style 3 (blouson + straight pants), style 4 (cardigan + half pants), style 5 (military jacket + straight pants), style 6 (loose fit jacket + skinny pants), and style 7 (baseball jumper + straight pants) were prepared in the evaluation questionnaire. The study compared male and female interest and knowledge of men's fashion, evaluated the sensibility difference depending on the men's fashion, analyzed whether there is a difference between preferred men's clothing and actual wearing of the clothing, and examined the preferred style in relation with the lifestyle. The results are as follows: First, men's fashion was diversified and subdivided, and interest and knowledge about men's fashion was greater for males than females. Second, sensibility of men's fashion had significant differences depending on the style, and it did not depend on genders. Third, there was a clear difference between the most favored style by the 20s and the actual style they commonly wear; the favored style and the actual worn style were consistent 66.1% of all the cases, inconsistent 33.9% of those. Style 3 had the highest preference and the actual wearing rate, and style 5 was the least preferred and worn. Fourth, the more extroverted lifestyle rather than introverted one, the more it was likely to prefer diverse styles.

An Analysis on the Contents of the Food Sanitation in the Primary, Middle and High School Textbooks (초, 중, 고교 교과서의 식품위생 내용 분석 연구)

  • 남철현;변부형;노병의;남원계
    • Journal of Food Hygiene and Safety
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    • v.9 no.2
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    • pp.95-104
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    • 1994
  • A study on food sanitation in school textbooks was performed for 7 months from October 1, 1992 through may 31, 1993. A total of 243 textbooks (77 from elementary school level, 52 from junior high school level and 114 from senior high school level) were reviewed and analyzed. This study was performed in order to develop better method for teaching the subjet of food sanitation to students effectively. The results are as follows: 1) A total number of pages of textbooks dealing with food sanitation were 48 pages (0.53%) in elementary level textbooks, 38.05 pages (0.31%) in junior high school level and 105.05 pages (0.34%) in senior high school level. 2) 104.05 pages were allocated for the subject of food storage, 33 pages for insect and rodent control, 32 pages for food poisoning, 15 pages for food contamination and 7 pages for kitchen hygiene. 3) Of the four categories of textbooks, the numbr of pages dealing with food sanitation were 145 in home economics, 31 in physical education, 12 in social studies and 3.0 in science. 4) Home economics and physical education textbooks allocated many pages in dealing with food sanitation. Home economics textbooks had 88 pages for food storage, 26.05 pages for food poisoning, 7 pages for food contamination, and 2 pages for kitchen hygiene. Physical education textbooks had 15 pages about food storage, 4 pages for food poisoning, 5 pages for food contamination, and 1 page for kitchen hygiene. 5) Social studies textbooks had 5 pages for food storage, 4 pages for insect and rodent control, 3 pages for food contamination. 6) The number of pages dealing with food sanitation in elementary school level textbooks were 20 in physical education, 13 in home economics, 12 in social studies, 3 in science respectively. 7) The number of pages dealing with food sanitation in junior high school level textbooks were 31.05 in home economics, and 7.00 in physical education textbooks. 31.50 pages were about food storage, 6 pages about food poisoning, 13 pages about insect and rodent control, and 1 page about kitchen hygiene. 8) The number of pages dealing with food sanitation in senior high school level textbooks were 101.05 in home economics and 4 in physical education textbooks. 64 pages were about food storage, 21.05 about food poisoning, 13 about insect and rodent control, 4 about kitchen hygiene, and 3 about food contamination. 9) Pictures, drawings and tables were used in explaining food sanitation in the textbooks, 32 drawings of a total of 38 cuts in elementary school level textbooks, 8 pictures of 10 cuts in junior high school level and 13 tables of 14 cuts in senior high school level were used. 10) 5th grade textbooks of elementary school did not have a subject on food poisoning. Other grade textbooks in elementary school level did not contain a subject on food contamination and insect and rodent control. It's recommended that these subjects be contained in the textbooks. 11) It is necessary to teach a subject on health or health and environment independently and contain food sanitation in the subject. It is recommended that a textbook on health or health and environment be published and taught to students. 12) It is recommended that teachers specialized in health education be assigened to schools to teach health related subjects. 13) It is recommended that book publishers use the latest information in the textbooks and technical terms be unified.

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Estrogeicity of Genistein and Bisphenol A (콩류식품의 주성분인 Genistein과 식품포장재 및 용기에 사용되는 Bisphenol A의 에스트로젠 효과에 관한 연구)

  • 강경선;이영순;신광순
    • Journal of Food Hygiene and Safety
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    • v.13 no.2
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    • pp.106-111
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    • 1998
  • This study has been focused on both estrogenic and proliferating activity of genistein (GEN) and bisphenol A (BPA). GEN and BPA enhance the proliferation of estrogen-dependent MCF-7 human breast cancer cells at concentrations as low as 100 nM of GEN and 8 ng/ml of BP A achieving similar effect to that of estradiol at 1 nM. Expression of the estrogen responsive gene, pS2 was also induced in MCF-7 cells by treatment with genistein at dose as low as 1 nM and BPA at dose as low as 4 ng/ml. Using 21 day-old ovariectomized nude mice, we examined end-bud formation and mammary gland development after treatment with bisphenol A or genistein. Compared with untreated control, mammary gland development and end-bud formation were significantly increased in mice fed genistein or bisphenol A (p<0.05). Taken together, it is concluded that GEN and BP A can act as an estrogen agonist resulting in cell proliferation and induction of the estrogen responsive pS2 gene in MCF-7 cells in vitro and in athymic mice in vivo, respectively. Therefore, it is suggested that GEN and BP A might modulate human endocrine system and these compounds might be considered as a endocrine modulator at the low levels of doses.

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Effect of Dye-absorbing Duration and Environmental Conditions on Quality of Preserved Leaves in Eucalyptus cinerea (염료 흡수기간 및 환경조건이 유칼립투스의 보존엽 품질에 미치는 영향)

  • Lim, Young Hee;Kim, So Eun;Oh, Wook
    • Horticultural Science & Technology
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    • v.32 no.3
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    • pp.390-399
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    • 2014
  • The objective of this study was to establish a processing technology for preserved leaves based on the results from the examination of the optimal period and condition for dye-absorbing treatment for Eucalyptus cinerea F. Mull. ex Benth. (silver dollar eucalyptus) being used frequently as plant material for flower design. Cut foliages of E. cinerea with uniformly matured leaves were cut into 20 cm lengths and their lower stem parts were placed in dye solution in growth chambers with different temperatures (10, 20, 30, and $40^{\circ}C$), vapor pressure deficits (VPD; 0.23, 0.70, 1.17, and 1.61 kPa), and photoperiods (0, 6, 12, 24 hours) for 3, 6, 9, and 12 days, and then dried in a room of $20^{\circ}C$ for three days. Lower temperature during preserving dye treatment reduced the changes in leaf color compared with fresh leaves and decreased ${\Delta}E$ value. Especially, high temperature increased red degree (a) and decreased yellow degree (b) due to browning. Lower VPD reduced the change in leaf color compared with fresh leaves and decreased ${\Delta}E$ value. Shorter photoperiod reduced the change in leaf color compared with fresh leaves and decreased ${\Delta}E$ value. The ${\Delta}E$ value increased with increasing absorbing duration under three environmental conditions. The flexibility of stem and leaves after dipped into preserving dye solution and dried for 3 days increased with decreasing temperature, VPD and dipping duration. Therefore, the optimal environment condition for dye treatment was 0.23-0.70 kPa VPD at $10-20^{\circ}C$ in the darkness, and the optimal and economical duration was 3 days. These conditions reduced the speed of water loss by decreasing transpiration, so yellowing or browning by rapid water loss deteriorated the quality of preserved leaves out of these ranges.

Effect of Salinity Stress on Growth, Yield, and Proline Accumulation of Cultivated Potatoes (Solanum tuberosum L.) (염 스트레스에 따른 감자 품종 (Solanum tuberosum L.) 간 생육, 수량 및 proline 함량 변이)

  • Im, Ju Sung;Cho, Ji Hong;Cho, Kwang Soo;Chang, Dong Chil;Jin, Yong Ik;Yu, Hong Seob;Kim, Wha Yeong
    • Horticultural Science & Technology
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    • v.34 no.6
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    • pp.818-829
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    • 2016
  • This study evaluated the responses of 18 potato cultivars to three levels of salinity stress (electrical conductivity, EC: 1.0, 4.0, and $8.0dS{\cdot}m^{-1}$). Stem, leaf, root, chlorophyll, tuber yield, and proline content were investigated and statistically analyzed using analysis of variance (ANOVA) and correlations. Stem number and stem diameter were not affected by salinity, but stem length and aerial weight showed highly significant responses to salinity. Aerial weight decreased with increasing salinity levels in most cultivars, while it increased in some the cultivars 'Daejima', 'Goun', 'Haryeong', and 'LT-8'. Leaf number, leaf area index, and leaf weight were most significantly affected by salinity and the cultivar ${\times}$ salinity interaction. Root length, root weight, total chlorophyll and chlorophyll a were affected by salinity, but not by the cultivar ${\times}$ salinity interaction. The opposite trend was shown in chlorophyll b. Although there was great variability among cultivars, tuber yield decreased in all cultivars, and was most significantly influenced by salinity and the cultivar ${\times}$ salinity interaction. 'Superior', 'Kroda', 'Romana', and 'Duback' gave better tuber yields under salinity at EC 4.0 and $8.0dS{\cdot}m^{-1}$ than the cultivars with better aerial weights. Proline content was increased by salinity in all cultivars, and was more remarkable in the cultivars with better aerial weights than in cultivars such as 'Superior' and 'Kroda' with better tuber yields. Leaf number, leaf area index, leaf weight, and root length parameters were considered to be useful criteria in the evaluation of salt tolerance because of their high positive correlation with tuber yield; however, given its negative correlation with tuber yield under high salinity, proline content was not. Salinity tolerances varied greatly among potato cultivars. The low correlation between growth and yields of aerial parts under high salinity suggests that, in commercial agriculture, it might be more practical to compare relative yields to controls. Additionally, 'Superior', 'Kroda', 'Romana', and 'Duback' might be very useful cultivars to use in breeding programs to develop salinity-tolerant potatoes, as well as for sustainable potato production in saline areas.

Design and Implementation of an Execution-Provenance Based Simulation Data Management Framework for Computational Science Engineering Simulation Platform (계산과학공학 플랫폼을 위한 실행-이력 기반의 시뮬레이션 데이터 관리 프레임워크 설계 및 구현)

  • Ma, Jin;Lee, Sik;Cho, Kum-won;Suh, Young-kyoon
    • Journal of Internet Computing and Services
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    • v.19 no.1
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    • pp.77-86
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    • 2018
  • For the past few years, KISTI has been servicing an online simulation execution platform, called EDISON, allowing users to conduct simulations on various scientific applications supplied by diverse computational science and engineering disciplines. Typically, these simulations accompany large-scale computation and accordingly produce a huge volume of output data. One critical issue arising when conducting those simulations on an online platform stems from the fact that a number of users simultaneously submit to the platform their simulation requests (or jobs) with the same (or almost unchanging) input parameters or files, resulting in charging a significant burden on the platform. In other words, the same computing jobs lead to duplicate consumption computing and storage resources at an undesirably fast pace. To overcome excessive resource usage by such identical simulation requests, in this paper we introduce a novel framework, called IceSheet, to efficiently manage simulation data based on execution metadata, that is, provenance. The IceSheet framework captures and stores each provenance associated with a conducted simulation. The collected provenance records are utilized for not only inspecting duplicate simulation requests but also performing search on existing simulation results via an open-source search engine, ElasticSearch. In particular, this paper elaborates on the core components in the IceSheet framework to support the search and reuse on the stored simulation results. We implemented as prototype the proposed framework using the engine in conjunction with the online simulation execution platform. Our evaluation of the framework was performed on the real simulation execution-provenance records collected on the platform. Once the prototyped IceSheet framework fully functions with the platform, users can quickly search for past parameter values entered into desired simulation software and receive existing results on the same input parameter values on the software if any. Therefore, we expect that the proposed framework contributes to eliminating duplicate resource consumption and significantly reducing execution time on the same requests as previously-executed simulations.

Preparation and Oxygen Permeation Properties of La0.07Sr0.3Co0.2Fe0.8O3-δ Membrane (La0.07Sr0.3Co0.2Fe0.8O3-δ 분리막의 제조 및 산소투과 특성)

  • Park, Jung Hoon;Kim, Jong Pyo;Baek, Il Hyun
    • Applied Chemistry for Engineering
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    • v.19 no.5
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    • pp.477-483
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    • 2008
  • $La_{0.7}Sr_{0.3}Co_{0.2}Fe_{0.8}O_{3-{\delta}$ oxide was synthesized by a citrate method and a typical dense membrane of perovskite oxide has been prepared using as-prepared powder by pressing and sintering at $1300^{\circ}C$. Precursor of $La_{0.7}Sr_{0.3}Co_{0.2}Fe_{0.8}O_{3-{\delta}$ prepared by citrate method was investigated by TGA and XRD. Metal-citrate complex in precursor was decomposed into perovskite oxide in the temperature range of $260{\sim}410^{\circ}C$ but XRD results showed $SrCO_3$ existed as impurity at less than $900^{\circ}C$. Electrical conductivity of membrane increased with increasing temperature but then decreased over $700^{\circ}C$ in air atmosphere ($Po_2=0.2atm$) and $600^{\circ}C$ in He atmosphere ($Po_2=0.01atm$) respectively due to oxygen loss from the crystal lattice. The oxygen permeation flux increased with increasing temperature and maximum oxygen permeation flux of $La_{0.7}Sr_{0.3}Co_{0.2}Fe_{0.8}O_{3-{\delta}$ membrane with 1.6 mm thickness was about $0.31cm^3/cm^2{\cdot}min$ at $950^{\circ}C$. The activation energy for oxygen permeation was 88.4 kJ/mol in the temperature range of $750{\sim}950^{\circ}C$. Perovskite structure of membrane was not changed after permeation test of 40 h and the membrane was stable without secondary phase change with 0.3 mol Sr addition.

Evaluation on Climate Change Vulnerability of Korea National Parks (국립공원의 기후변화 취약성 평가)

  • Kim, Chong-Chun;Kim, Tae-Geun
    • Korean Journal of Ecology and Environment
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    • v.49 no.1
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    • pp.42-50
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    • 2016
  • The purpose of this study is to set the direction to manage national parks to cope with climate change, and offer basic data to establish the relevant policies. Towards this end, this study analyzed the current and future climate change vulnerability of national parks using the 24 proxy variables of vulnerability in the LCCGIS program, a tool to evaluate climate change vulnerability developed by the National Institute of Environmental Research. To analyze and evaluate the current status of and future prospect on climate change vulnerability of national parks, the proxy variable value of climate exposure was calculated by making a GIS spatial thematic map with $1km{\times}1km$ grid unit through the application of climate change scenario (RCP8.5). The values of proxy variables of sensitivity and adaptation capability were calculated using the basic statistics of national parks. The values of three vulnerability evaluation items were calculated regarding the present (2010s) and future (2050s). The current values were applied to the future equally under the assumption that the current state of the proxy variables related to sensitivity and adaptation capability without a future prediction scenario continues. Seoraksan, Odaesan, Jirisan and Chiaksan National Parks are relatively bigger in terms of the current (2010s) climate exposure. The national park, where the variation of heat wave is the biggest is Wolchulsan National Park. The biggest variation of drought occurs to Gyeryongsan National Park, and Woraksan National Park has the biggest variation of heavy rain. Concerning the climate change sensitivity of national parks, Jirisan National Park is the most sensitive, and adaptation capability is evaluated to be the highest. Gayasan National Park's sensitivity is the lowest, and Chiaksan National Park is the lowest in adaptation capability. As for climate change vulnerability, Seoraksan, Odaesan, Chiaksan and Deogyusan National Parks and Hallyeohaesang National Park are evaluated as high at the current period. The national parks, where future vulnerability change is projected to be the biggest, are Jirisan, Woraksan, Chiaksan and Sobaeksan National Parks in the order. Because such items evaluating the climate change vulnerability of national parks as climate exposure, sensitivity and adaptation capability show relative differences according to national parks' local climate environment, it will be necessary to devise the adaptation measures reflecting the local climate environmental characteristics of national parks, rather than establishing uniform adaptation measures targeting all national parks. The results of this study that evaluated climate change vulnerability using climate exposure, sensitivity and adaptation capability targeting Korea's national parks are expected to be used as basic data for the establishment of measures to adapt to climate change in consideration of national parks' local climate environmental characteristics. However, this study analyzed using only the proxy variables presented by LCCGIS program under the situation that few studies on the evaluation of climate change vulnerability of national parks are found, and therefore this study may not reflect overall national parks' environment properly. A further study on setting weights together with an objective review on more proper proxy variables needs to be carried out in order to evaluate the climate change vulnerability of national parks.