• Clinical and Experimental Reproductive Medicine
• /
• v.22 no.2
• /
• pp.131-141
• /
• 1995

This study was designed to determine the relationship between sperm acrosome reaction following ionophore challenge(ARIC) and hamster ovum sperm penetration assay(SPA) as assessment of fertilizing capacity of male. ARIC test and SPA were performed in 23 fertile and 19 subfertile men. The results were as follows; Sperm concentration was significantly higher in fertile group compared with subfertile group: $114.6{\pm}64.40$ vs $61.3{\pm}46.50{\times}10^6/ml$. However, there were no significantly differences in seminal volume, motility and motility index, respectively. There was a significantly correlation between spontaneous and induced AR in fertile and subfertile group, respectively. ARIC value was significantly higher in fertile group, compared with subfertile group: $12.0{\pm}5.57%$ vs $2.6{\pm}4.96%$. Both Penetration rate(PR) and Penetration index(PI) were significantly higher in fertile group, compared with subfertile group: $97.4{\pm}7.40%$ vs $64.9{\pm}36$. 20% and $5.4{\pm}2.88$ vs $1.5{\pm}1.47$, respectively. The Positive predictive value(PPV), Negative predictive value(NPV), sensitivity and specificity of ARIC test (cut-off: 8.5) and SPA(PI cut-off : 3.0) in predicting fertility were 95.0%, 81.8%, 82.6%, 94.7% and 95.2%, 85.7%, 87.0% and 94.7%, respectively. There was no significantly difference in predicting fertility between ARIC test and SPA. In conclusion, ARIC test was shown to have a predictive value for fertilizing capacity comparable to that of the hamster ovum sperm penetration assay. Therefore, ARIC test may be a simple and cost-effective addition to existing semenology instead of SPA.

• Clinical and Experimental Reproductive Medicine
• /
• v.24 no.3
• /
• pp.335-340
• /
• 1997

Fluorescence in situ hybridization (FISH) techniques allow the enumeration of chromosome abnormalities and from a great potential for many clinical applications. In order to produce quantitative and reproducible results, expensive tools such as a cooled CCD camera and a computer software are required. We have developed a Chromosome Image Processing System (Chips) using FISH that allows the detection and mapping of the genetic aberrations. The aim of our study, therefore, is to evaluate the capabilities of our original system using a black-and-white video camera. As a model system, three repetitive DNA probes (D18Z1, DXZ1, and DYZ3) were hybridized to variety different clinical samples such as human metaphase spreads and interphase nuclei obtained from uncultured peripheral blood lymphocytes, uncultured amniocytes, and germ cells. The visualization of the FISH signals was performed using our system for image acquisition and pseudocoloring. FISH images were obtained by combining images from each of probes and DAPI counterstain captured separately. Using our original system, the aberrations of single or multiple chromosomes in a single hybridization experiment using chromosomes and interphase nuclei from a variety of cell types, including lymphocytes, amniocytes, sperm, and biopsied blastomeres, were enabled to evaluate. There were no differences in the image quality in accordance with FISH method, fluorochrome types, or different clinical samples. Always bright signals were detected using our system. Our system also yielded constant results. Our Chips would permit a level of performance of FISH analysis on metaphase chromosomes and interphase nuclei with unparalleled capabilities. Thus, it would be useful for clinical purposes.

• Polymer(Korea)
• /
• v.37 no.4
• /
• pp.518-525
• /
• 2013

To improve the mechanical properties of polypropylene (PP) biocomposites reinforced with sulfuric acidtreated green algae (SGA), SGA/graphite nanoplatelets (GNP)/PP biocomposites were prepared and their properties were evaluated depending on the particle size and content of GNP. The flexural and impact strength of SGA/GNP/PP biocomposites decreased with the addition of GNP, whereas the flexrual and storage moduli were greatly improved with increasing GNP loading. SGA/GNP/PP biocomposites reinforced with GNP5 showed generally better mechanical properties compared to that reinforced with GNP15 mainly due to the improved dispersion of the smaller GNP. SGA/GNP/PP biocomposites reinforced with GNP5 showed a lower resistance to the thermal expansion because the relatively uniform dispersion of smaller GNP was responsible for the effective heat transfer to the polymer matrix. As a result, SGA/GNP/PP biocomposite was acceptable for the general purpose application due to the improved flexural resistance, storage moduli, and damping characteristics.

• Journal of the Society of Cosmetic Scientists of Korea
• /
• v.34 no.1
• /
• pp.51-55
• /
• 2008

Although phthalates aren't used as an cosmetic ingredient, some cosmetics especially nail lacquer, hair spray, and perfume still have phthalates. This is mainly caused by contamination and carryover during manufacturing process, so analysis of phthalates in those cosmetics has became a very important thing for quality-assurance(Q.A). The main phthalates under debate are diethyl phthalate(DEP), dibutyl phthalate(DBP), and bis(2-ethylhexyl) phthalate (DEHP) in domestic market. Gas chromatography-mass spectrometry(GC-MS) coupled with solvent extraction and concentration has been used for ppm level and sub ppm level analysis of phthalates. It requires much time and cost to use mass spectrometric detector and to prepare the test solution. Moreover analysis of phthalates at low concentrations is difficult because of contamination which results in wrong analytical results. In the present study, we showed a simple method using gas chromatography-flame ionization detector(GC-FID) which has fast analysis time, minimum use of solvent, reduced sample preparation steps for minimizing contamination and quantitative range of $2{\sim}50{\mu}g/g(ppm)$ in products. Consequently, this method will be proper for Q.A analysis in related companies.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.40 no.2
• /
• pp.73-79
• /
• 2020

This study was conducted to develop a technique for the stable production of Italian ryegrass(IRG) seeds in reclamation sites. Harvesting 35 days after heading in Autumn resulted in the highest production, an average of 2,232kg/ha. The production yield decreased due to seed loss as harvesting was delayed to 45 and 55 days post-heading. For the harvested 35 days post-heading, under growing rice sowing resulted in 80% seed yield of after rice sowing's; spring sowing resulted in 40%. After rice sowing using the domestic IRG seeds of 30kg/ha produced the highest seed yield of 2,507kg/ha. The highest straw yield has resulted when using the imported IRG seeds of 20kg/ha for after rice sowing 35 days post-heading fresh weight 36,667kg/ha, dry weight 14,500kg/ha, and TDN weight 7,895kg/ha.

• Korean Journal of Construction Engineering and Management
• /
• v.15 no.4
• /
• pp.128-138
• /
• 2014

Development of infrastructure component libraries is a critical requirement for the accelerated adoption of BIM in the civil engineering sector. Libraries reduce the time for BIM model creation, allows accurate quantity take offs, and shared use of standard models in a project. However, such libraries are currently in very short supply in the domestic infrastructure domain. This research introduces library components for retaining walls and box culverts generated from 2D standard drawings made publicly available by MOLIT. Commercial BIM software was used to create the concrete geometry and rebar, and dimensional/volumetric parameters were defined to maximize the reuse and generality of the libraries. Use of the these libraries in a project context demonstrates that they allow accurate and quick quantity take offs, and easier management of geometric information through the use of a single library as to numerous 2D drawings. It also demonstrates the easy modification of the geometries of the components if and when they need to changed. However, the application also showed that some of the rebar components (stirrups and length wise rebars) do not get properly updated when concrete geometries are changed, demonstrating the limits of current software applications. The research provides evidence of the many advantages of using BIM libraries in the civil engineering, thus providing the incentive for further development of standard libraries and promoting the use of BIM in infrastructure projects.

• Korean Chemical Engineering Research
• /
• v.48 no.2
• /
• pp.224-231
• /
• 2010

In GvdW EOS, a recently presented paper, shows that the characteristic status for spherical non-linear particle, of which the mutual behavior is known to be vdWf(van der Waals force) only, could be described well enough in the critical region. However, in current papers, analysis has not been done on GvdW about whether it is accurate or not, even for the particles in the linear form or those with the additional mutual behavior such as static-electricity, so there's some argument about the wide use of that. Therefore, in this paper, for the simulation in the critical region of Normal-alkane group(R=methane, ethane, propane, butane) which are the particles that has a linear charateristic and Normal-amine group($RNH_2$, R=methyl-, ethyl-, propyl-amine) where static-electricity is extremely shown, GvdW parameter values about these particles are defined, and based on this simulation, we compared results to the current EOS presented recently, and analyzed them. Through the simulation, it was shown that in case of Normal-alkane group and Normal-amine group molecules, GvdW presents an accurate critical region characteristic which is far more close to the measurement compared to current EOSs. Especially for butane with big amount in molecules, we found out that only GvdW EOS can reach close enough to the critical point.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.37 no.7B
• /
• pp.578-585
• /
• 2012

Location tracking system through GPS and Wi-Fi is available at no additional cost in an environment of IEEE 802.11-based wireless network. It is useful for many applications in outdoor environment. However, a previous systems used for general device to tag. It is unsuitable for power aware location tracking system because general devices is more expensive and non-optimized for tracking. The hand-off method of IEEE 802.11 standard is not enough considering power consumption. This thesis analyzes the previous location tracking systems and proposes power aware system. First, we designed and implemented tag to optimize location tracking. Next, we propose low-power hand-off method and low-power behavior model in implemented tag. The proposed hand-off method resolve power problem by using the location information and behavior model minimize power consumption of tag through power-saving mode and the concept of duty cycle. To evaluating proposed methods and system performance, we perform simulations and experiments in real environment. And then, we calculate tag's power consumption based on the actual measured current consumption of each operation. In a simulation result, the proposed behavior model and hand-off method reduced about 98%, 59% than the standard's hand-off and default behavior model.

• Journal of Life Science
• /
• v.15 no.4 s.71
• /
• pp.553-560
• /
• 2005

The stf (Salmonella typhimurium fimbriae) operon consisting of stfA(CDEFG assumes to encode putative fimbriae. The complete stf operon is existed in S. typhimurium and S. choleraesuis, whereas it is absent in S. typhi. Analyses of the amino acid residues between major subunit StfA of the Stf fimbriae and those of known other fimbriaes suggested that Stf belongs to class I type fimbriae. Through comparison of StfD chaperone with the other fimbrial chaperones, and of C-terminus in subunits of Stf fimbriae, it belongs to FGS (with a short Fl-G1 loop) subfamily. In order to investigate the expression of stf operon, we have constructed a Salmonella strain containing a chromosomal stfA::lacZYA transcriptional fusion, resulting in S. typhimurium $_X8532$. The strain $_X8532$ lacked the expression of \beta-galactosidase$ under normal culture conditions. However, with longer incubation time of the S. typhimurium $_X8532$, we have isolated 21 individual strains exhibiting $Lac^+$ phenotype. $Lac^+$ phenotype was appeared as approximately 0.03 frequency per generation. All isolates expressed lacZ constitutively in the various environmental conditions. Various global regulatory proteins including RpoS, OmpR, and CpxR were not involved in the regulation of the stf operon. A S. typhimurium $_X8661$ mutant lacking stfAC function attenuated 6.7 folds more than that of wild type $_X3761$ in the mouse virulence test, suggesting in the somehow involved in the Salmonella pathogenesis.

• Journal of the Microelectronics and Packaging Society
• /
• v.23 no.3
• /
• pp.63-67
• /
• 2016

In this paper, we present an indirect method of elastic modulus measurement for various lamination layers formed on polymer-based compliant substrates. Although the elastic modulus of every component is crucial for mechanically reliable microelectronic devices, it is difficult to accurately measure the film properties because the lamination layers are hardly detached from the substrate. In order to resolve the problem, 3-point bending test is conducted with a film-substrate specimen and area transformation rule is applied to the cross-sectional area of the film region. With known substrate modulus, a modulus ratio between the film and the substrate is calculated using bending stiffness of the multilayered specimen obtained from the 3-point bending test. This method is verified using electroplated copper specimens with two types of film-substrate structure; double-sided film and single sided film. Also, common dielectric layers, prepreg (PPG) and dry film solder resist (DF SR), are measured with the double-sided specimen type. The results of copper (110.3 GPa), PPG (22.3 GPa), DF SR (5.0 GPa) were measured with high precision.