• Title/Summary/Keyword: 백구

Search Result 72, Processing Time 0.024 seconds

Ar 및 He기체유입에 따른 저온 대기압 DBD플라즈마에 의한 E.Coli의 노출 효과

  • Lee, Sang-Hak;Baek, Gu-Yeon;Kim, Yong-Hui;Yu, Yeong-Hyo;Choe, Eun-Ha
    • Proceedings of the Korean Vacuum Society Conference
    • /
    • 2012.02a
    • /
    • pp.512-512
    • /
    • 2012
  • 최근 저온 대기압 플라즈마 장치의 개발로 대기 및 수질 환경, 바이오 메디컬분야로의 응용 연구가 활발히 진행되어 공기 중 플라즈마의 살균 및 정화효과에 대한 많은 결과가 발표되어 왔다. 본 연구는 면방전 구조의 DBD플라즈마 소스를 제작하여 He과 Ar 기체를 유입하여 미생물인 E.Coli의 변화를 관찰하였다. 면방전 구조의 DBD플라즈마 소스는 1.8 mm 두께의 유리기판위에 포토리소그라피 공정으로 미소전극을 형성하여 고밀도의 방전 셀을 형성하였으며 방전시 발생하는 열 효과를 제어하기 위하여 냉각장치를 제작하여 장착했다. 또한 유리기판과 포토 리소그라피 공정은 방전영역에 제한없이 다양한 크기의 소스제작이 가능하다. 셀 피치가 $400{\mu}m$이며 $cm^2$ 당 200여개의 방전 셀로 구성되어 있어서 기존 메쉬타입의 DBD플라즈마 장치에 비해 균일하게 플라즈마를 조사할 수 있으며 플라즈마 제트 장치에 비해서는 넓은 면적을 동시에 조사할 수 있게 되었다. Ar 과 He기체를 3 L/min의 유량으로 방전공간에 유입하면서 1kV의 구동전압으로 플라즈마를 발생 하였으며, 플라즈마의 조사시간을 20 s, 40 s, 60 s 간격으로 변화를 주어 E.Coli의 변화를 관찰하였다.

  • PDF

Biophoton Emission of MDCK Kidney Cell with ROS(reactive oxygen species) (Biophoton에 의한 생체 세포수준에서의 항산화 작용에 대한 척도)

  • 백구연;천병수;임재관;이승호;소광섭
    • KSBB Journal
    • /
    • v.18 no.3
    • /
    • pp.170-173
    • /
    • 2003
  • This study was studies biophoton characteristics of Madin-Darby canine kidney cells under the influence of CsA and each cell type (mock, wt, R55A) by employing a Photomultipliertube. $\textrm{H}_2\textrm{O}_2$ was used for producing reactive oxygen species (ROS) in this measurement. ROS is also generated during oxidative metabolism in living organism. Images from a fluorescence show an increase of photon intensity emitted from the sample on the influence of CsA and each cell type (mock, wt, R55A). It is believed chemiluminescence (CL) occurred by ROS is responsible for the biophoton emission. hence PMT measurement might be considered as a useful tool for studying biochemical characteristics in relation to ROS.

Variation of chemical Composition and Relative migration of major Elements in the weathering of Baeg-Gu Granite (백구화강암(白鷗花崗巖)의 풍화(風化)에 따른 화학조성(化學組成)의 변화(變化)와 주요원소(主要元素)의 상대적(相對的) 이동(移動))

  • Nam, Ki Sang
    • Economic and Environmental Geology
    • /
    • v.7 no.4
    • /
    • pp.175-184
    • /
    • 1974
  • Weathering of granites has a great geochemical significance, because of their chemical stability near the earth surface which is more pronounced than in most other rocks. The author intended to observe the relative mobility of major elements in weathering process of the granite, distributed on outskirts of Iri city Jeolla-bugdo, Korea. He analysed fresh granites and weathered ones from the Baeg-Gu granite mass and obtained following conclusions in the triangle-diagrams and the oxidized variation diagrams of the samples. 1) The increasing phenomena of $H_2O$ was observed clearly in early and late stages of weathering process. 2) The early stage of weathering is commenced by physical weathering and followed by chemical weathering. 3) The ratio of $FeO/Fe_2O_3$, FeO/MgO, and $SiO_2/Al_2O_3$ decreased uniformly from early to late stage of weathering processes. 4) It was proved that weathering potential of granite was larger than that of basaltic rock. 5) The order of mobility in major elements was Ca, Na and K

  • PDF

Two Cases of Giant Cell Tumor of the Bone Diagnosed by Fine Needle Aspiration Cytology (골 거대세포종의 2예 세침 천자 세포학적 소견)

  • Myong, Na-Hye;Ha, Chang-Won;Cho, Kyung-Ja;Jang, Ja-June;Baek, Goo-Hyun;Lee, Soo-Yong
    • The Korean Journal of Cytopathology
    • /
    • v.1 no.1
    • /
    • pp.93-97
    • /
    • 1990
  • Two cases of giant cell tumor of bone diagnosed by fine needle aspiration cytology are described. Case 1 was a 28-year-old male who had pain sense for one year at the right distal thigh. His radiologic finding revealed a destructive cortical lesion with soft tissue extension at medial side of epiphysis of the distal femur. Case 2 was a 21-year-old female complaining pain at left distal forearm for eight months and showed a well-demarcated expansile osteolytic lesion with multiseptation, and cortical destruction at epiphysis and metaphysis of the left distal radius on the X-ray. Fine needle aspiration of each lesion was performed. The aspirate of the case 1 revealed moderate cellularity, which was composed of scattered giant cells of osteoclastic type and small round to oval monotonous stromal cells in large areas. Giant cells were evenly distributed in single or small groups and had irregular but abundant cytoplasms with 10 to 20 nuclei in the center. The nuclei showed ovoid shape, fine granular chromatin, and a small but conspicuous nucleolus. Stromal cells were dispersed in isolated pattern or sometimes aggregated in clusters and showed the same nuclei as those of giant cells and scanty cytoplasms. Comparing to case 1, case 2 had a more translucent abundant cytoplasm in the giant cells and more spindled stromal cells. All two cases revealed neither nuclear atypism nor increased abnormal mitoses In both giant and stromal cells, suggesting no evidence of malignancy. Thereafter the lesions were treated with excision and curettage, and histologically confirmed as giant cell tumors of the bone.

  • PDF

Sural Artery Flap for the Treatment of Soft Tissue Defects around Knee Joint (비복 동맥 피판을 이용한 슬관절 주위의 연부 조직 결손의 치료)

  • Bae, Kee Jeong;Lee, Young Ho;Kim, Min Bom;Lee, Hyuk Jin;Kwon, Ji Eun;Baek, Goo Hyun
    • Archives of Reconstructive Microsurgery
    • /
    • v.21 no.2
    • /
    • pp.143-148
    • /
    • 2012
  • Purpose: This report presents the authors' experience of twelve patients with sural artery flap for soft tissue defects around the knee joint. Materials and Methods: The patients' age ranged from 25 to 80 years; seven of the patients were male and five were female. The cause of soft-tissue defects involved wide excision for malignant soft tissue tumor, tumor prosthesis related infection, infection after total knee arthroplasty and chronic osteomyelitis. Postoperative range of motion was checked. The sensibility of flap was evaluated by Semmes-Weinstein monofilaments and two-point discrimination. Results: All flaps survived and provided satisfactory coverage of the defect. There was no complication except one delayed skin graft incorporation at donor site. Seven knee joints which had been stiff previously gained average 58 degrees of ROM postoperatively. All flaps retained sensibility and showed no significant increase in sensory thresholds comparing with contralateral side. Conclusion: Sural artery flap not only shows high survival rate and broad coverage ability, but also offers improvement in range of motion and preservation of sensation. We speculate that sural artery flap is valuable for the reconstruction of the soft tissue defects around knee joint.

  • PDF

Surgical Treatment of Congenital Brachymetatarsia According to the Number of Affected Rays (이환된 열 수에 따른 선천성 중족골 단축증의 수술적 치료)

  • Chung, Moon-Sang;Baek, Goo-Hyun;Gong, Hyun-Sik;Oh, Joo-Han;Lee, Young-Ho;Yoon, Pil-Whan;Kim, Ji-Hyeung
    • Journal of Korean Foot and Ankle Society
    • /
    • v.10 no.1
    • /
    • pp.24-30
    • /
    • 2006
  • Purpose: To present our treatment protocol and surgical outcome for patients with congenital brachymetatarsia in which treatment was decided according to the number of affected rays. Materials and Methods: Sixty-nine metatarsals in 44 patients with single or multiple congenital brachymetatarsia were included in the study. When a single ray was affected in a foot, we performed a one-stage lengthening using an intercalary autogenous iliac bone graft. We overcame excessively short rays by the double level lengthening at the metatarsal and proximal phalanx as one stage. When multiple rays were affected in one foot, we performed a one-stage combined shortening and lengthening procedure without an iliac bone graft. Results: All patients were satisfied with the cosmetic and functional results. The average length gain by one-stage lengthening in 56 metatarsals of 38 patients was 14 (6-21) mm. Six patients with a combined shortening and lengthening procedure regained a nearly normal parabola of the involved foot. Neurovascular complication was not identified. Conclusion: Satisfactory results were achieved for the treatment of patients with congenital brachymetatarsia, by individualizing the surgical options according to the number of affected rays and general foot appearance.

  • PDF

Medial Epicondylectomy for the Treatment of Cubital Thnnel Syndrome - A Retrospective Comparison with Anterior Subcutaneous Transposition - (주 관 증후군의 수술적 치료-내상과 절제술과 척골 신경 피하 전방 전위술의 결과 비교-)

  • Chung Moon-Sang;Baek Goo-Hyun;Kim Sang-Lim;Park Young-Chun
    • Clinics in Shoulder and Elbow
    • /
    • v.1 no.1
    • /
    • pp.100-108
    • /
    • 1998
  • Surgical treatment of cubital tunnel syndrome has been reported according to a wide variety of techniques since the end of the last century. Theses range from simple decompression to various forms of nerve transposition and medial epicondylectomy. However, we could find only few reports which compare the results between different types of operations. The treatment results of medial epicondylectomy and anterior subcutaneous transposition, were analysed retrospectively. From March 1984 to January 1996, a total of 110 patients had operations for cubital tunnel syndrome. Seventy four of them were followed-up for more than one year, and only they were included in this study. Anterior subcutaneous transposition was performed in 26 patients; and medial epicondylectomy in 48 patients. There were 52 males and 22 females, with an average age of 34 years (range, 13 to 75). The average follow-up period was 40 months (range, 12 to 132). Grading system by Gabel and Amadio were used for evaluation of the patients; pain, sensory and motor dysfunction were checked preoperatively and at last follow-up. In 26 patients of anterior subcutaneous transposition, 7 (27%) were graded as excellent, 11 (42%) good, 3 (12%) fair and 5 (19%) poor. In 48 patients of medial epicondylectomy, 16 (34%) were excellent, 27 (56%) good, 3 (6%) fair and 2 (4%) poor. Sixty nine percent were excellent or good in anterior subcutaneous transposition group, while ninty percent in medial epicondylectomy group. This difference was significant statistically (p<0.01 )., From our experiences, we suggest medial epicondylectomy for the treatment of cubital tunnel syndrome, rather than anterior subcutaneous transposition.

  • PDF

Fluorescent Method for Observing Intravascular Bonghan Duct (형광염색을 이용한 혈관내봉한관의 관찰)

  • Lee, Byung-Cheon;Baik, Ku-Youn;Johng, Hyeon-Min;Sung, Baeck-Kyoung;Soh, Kyung-Soon;Kang, Dae-In;Soh, Kwang-Sup
    • Journal of Pharmacopuncture
    • /
    • v.8 no.3
    • /
    • pp.5-9
    • /
    • 2005
  • Observation of intra-vascular threadlike structures in the blood vessels of rats is reported with the images by differential interference contrast microscope, and fluorescence inverted microscope of the acridine-orange stained samples. The confocal microscope image and the hematoxylin-eosin staining revealed the distinctive pattern of nuclei distribution that clearly discerned the threadlike structure from fibrin, capillary, small venule, arteriole, or lymph vessel. Physiological function of the intra-vascular thread in connection with acupuncture is discussed. Especially, this threadlike duct can be a circulation path for herb-liquid flow, which may provide the scientific mechanism for therapeutic effect of herbal acupuncture.

Argyrophilic Fibers of Intravascular Threadlike Structures in Rat (흰쥐 혈관 내에 존재하는 봉한관의 호은성 섬유)

  • Lee, Byung-Cheon;Nam, Tae-Jeong;Johng, Hyeon-Min;Park, Eun-Sung;Baik, Ku-Youn;Sung, Baeck-Kyoung;Soh, Kyung-Soon;Yoon, Yeo-Sung;Soh, Kwang-Sup
    • Journal of Pharmacopuncture
    • /
    • v.11 no.3
    • /
    • pp.5-16
    • /
    • 2008
  • Objective: We took intravascular threadlike structures from rat aortas to investigate their histological characteristics consistent with the intravascular Bonghan duct. Methods: Gomori s silver impregnation method, in addition to routine hematoxylin and eosin staining, was applied to demonstrate the characteristic feature of the intravascular threadlike structures. Results: These two staining methods clearly showed that the intravascular threadlike structures had unique features of argyrophilic reticular fibers and heavily stained oval or rod-shaped nuclei in them. Conclusion: The results are strong evidences for identifying threadlike structure as the intravascular Bonghan duct.

Surgical Treatment of Metastatic Bone Tumor in Extremity (사지에서 전이성 골종양의 수술적 치료)

  • Lee, Han-Koo;Lee, Sang-Hoon;Baek, Goo-Hyun;Lee, Chang-Seop
    • The Journal of the Korean bone and joint tumor society
    • /
    • v.1 no.1
    • /
    • pp.45-51
    • /
    • 1995
  • The incidence of metastatic bony lesion has been increased recently, with the advanced therapeutic modalities of malignant tumors. The purpose of this study was to evaluate the effectiveness of surgical treatment in impending or established pathologic fractures due to metastasis in extremity. From 1981 to 1992, thirty two patients who had established or impending pathologic fractures in extremity had been treated surgically. The locations of metastasis, were lower extremity in 24 cases(femsral head and neck;5, intertrochanteric;7, subtrochanteric;8, femoral shaft;4) and upper extremity in 8 cases(proximal humerus;2, humoral shaft;6). The method of surgery was excision of tumor mass and rigid internal fixation to allow immediate mobilization, except 2 cases of amputation. The results were analysed by two criteria. The first was the grade of performance status that was made by modification of Functional Classification of New York Heart Association. The second was the degree of pain relief. The performance status was improved at least one grade in all patients and the mean improvement was 1.7 grades. The satisfactory pain relief was achieved in 81% of patients. Postoperatively, most of the patients had only slight to moderate limitation of physical activities and they became comfortable at rest and ordinary activities. The solitary metastatic lesions showed better pain relief(92%) than multiple metastasis(75%). The curative surgery was done in 3 cases, and they showed more than 24 months of disease-free survival.

  • PDF