• Horticultural Science & Technology
• /
• v.32 no.2
• /
• pp.193-201
• /
• 2014

We investigated the effect of preharvest spray of aminoethoxyvinylglycine (AVG) on the fruit quality and incidence of physiological disorders during shelf-life of 'Hanareum' and 'Wonhwang' pear (Pyrus pyrifolia Nakai). AVG applications (0, 75, 150 and $300mg{\cdot}L^{-1}$) made 30 days before anticipated harvest of respective cultivar. AVG did not influence the fruit development and quality parameters including starch content, soluble solids and acidity at harvest time in two pear cultivars although the flesh firmness of the fruits treated with $300mg{\cdot}L^{-1}$ AVG were higher than that of control in 'Hanareum' pear. Among the three AVG concentrations, only $300mg{\cdot}L^{-1}$ AVG showed effectiveness on maintaining flesh firmness in 'Hanareum' pear during shelf-life. Meanwhile, competitive high flesh firmness was attained in 'Wonhwang' pear treated with $150mg{\cdot}L^{-1}$ AVG (27.7 N) and $300mg{\cdot}L^{-1}$ AVG (28.2 N) when compared with untreated control (17.8 N) during 21 days of shelf-life at $25^{\circ}C$. AVG did not cause any significant difference at skin color development relative to control in two pear cultivars. AVG decreased ethylene production and respiration rate at $300mg{\cdot}L^{-1}$ level in two pear cultivars during the entire shelf-life. During shelf-life, low incidence of physiological disorders including internal and core browning and mealiness were attained in the fruits treated at $300mg{\cdot}L^{-1}$ AVG in two cultivars when we compared with the untreated control.

• Korean Journal of Agricultural Science
• /
• v.21 no.2
• /
• pp.81-91
• /
• 1994

This study was conducted to find a potential effect of ethephon or dichlorprop spray on the fruit growth and maturation in 'Niitaka' pears. Chemicals were applied between June 10 to 24 (7-9 weeks after full bloom). Ethephon in the range from 25 ppm to 100 ppm, and dichlorprop from 20 ppm to 40 ppm reduced the fruit size regardless application time and concentration. The fruit growth was more inhibited at the higher concentrations in both chemicals. Flesh firmness was more rapidly decreased in the fruit treated by both chemicals. Firmness at harvest was lowest in the treatment of 100 ppm ethephon. The climacteric increase of ethylene synthesis occurred earlier in the fruit treated by ethephon or dichlorprop regardless application concentrations and the maximum peak of ethylene production was rugher. Total phenolics significantly reduced in fruits treated with ethephon. However, the contents of soluble solids and acid were not affected by ethephon or dichlorprop treatment. Ground color was changed rapidlys by the treatments resulting in the early maturation of fruit. Optimum maturity of fruit was shortened 2-3 days by ethephon and 3-4 days by dichlorporp when maturity was determined on the basis of ground color development and firmness.

• Journal of Bio-Environment Control
• /
• v.26 no.2
• /
• pp.64-71
• /
• 2017

The study was performed on the effects of crop load of pear (Pyrus pyrifolia Nakai) trees on shoot growth, reserved nutrients, and fruit production for two years, as well as regrowth of cuttings treated with various cold temperatures in 2012 and 2013. Crop load adjustments included three levels of fruiting; 54 (60%), 90 (100%), and 126 fruits per tree (140%). As increasing crop load from 60% to 140%, water sprout growth decreased, and one-year old shoot resulted in declined concentrations of total carbon, macro-nutrients, and free sugar. The 140% of crop load greatly increased fruit yield of approximately 35 tons per ha in both 2011 and 2012 but slightly reduced weight, size, and soluble solid contents in fruit. However, 140% of crop load increased annual production income to approximately over 10 million won compared to the 60% and 100% of crop load. Germination rates in the cuttings did not rapidly decrease up to $-20^{\circ}C$ in a growth chamber condition. The 140% of crop load decreased germination rates less than 70% of cuttings grown under $-30^{\circ}C$. Amounts of electrolyte leakage in the cuttings under various cold temperatures were not significantly different among the adjustment of crop load.

• Korean Journal of Environmental Agriculture
• /
• v.33 no.3
• /
• pp.198-204
• /
• 2014

BACKGROUND: Caused by cultural environment, the fruit skin stain results in serious damages to pear fruit. Particularly susceptible to this damage, 'Niitaka' pear accounts for 82% of pear cultivation in Korea and many farmers growing the pear trees have suffered economic losses due to fruit skin stain. This study investigated the effect of different treatments of 'Niitaka' pear during growing period on the occurrence of fruit skin stain. METHODS AND RESULTS: The treatments in the field included gibberellin (GA) paste, spraying with amino acid tree fertilizer, functional bagging, and coating of the inner paper bag with agents. The relationships between tree vigor, mineral nutrition concentration and fruit skin stain occurrence were also investigated. The fruit skin stain symptoms occurred from young fruit (May 25) until harvest. There was no exposed fruit flesh. The occurrence of fruit skin stain was significantly reduced in normal tree (shoot length 110 cm), as well as using GA paste treatment, and bagging in calcium and lime sulfur coated bags. However, spraying with amino acid tree fertilizer made no difference in comparison to control. In addition, bags in which the inner paper was coated with lime sulfur and soybean oil resulted in chemical injury to the fruit skin caused by bagging. The K concentration of shoot wood and fruit skin were higher than those of the control. Also, there were lower T-N, K concentration of leaf. CONCLUSION: These results suggest that occurrence of fruit skin stain in 'Niitaka' pear fruits during the growing period can be reduced by GA paste and bagging in calcium and lime sulfur coated bags. The symptoms of chemical injury to the fruit skin caused by bagging in lime sulfur and soybean oil coated inner paper were different compared to skin stain occurring in fruit during the growing period.

• Food Science and Preservation
• /
• v.23 no.6
• /
• pp.765-771
• /
• 2016

Omija-cheong, concentrated extracts from sugar-treated Omija fruit (Schisandra chinensis Baillon), is produced by traditional manner in Korea. The quality characteristics of Omija-cheong processed at low temperature with a pilot-scale were investigated to optimize the incubation time. With increasing incubation time in processing Omija-cheong, the pH level of Omija-cheong remained constant, while titratable acidity and organic acids increased. Fresh Omija fruits contained citric, malic and succinic acids, most of which were extracted into concentrated extracts after 37 days of incubation and reached to the stable concentration after 47 days of incubation. Titratable acidity in Omija-cheong gradually increased from 1.18% to 2.71%, and also was correlated with total concentration of organic acids. About 80% of supplemented sucrose for manufacturing Omija-cheong was converted into glucose and fructose until 68 days of incubation, and the composition of free sugars was maintained to be stable up to 138 days of incubation. The contents of total flavonoids and phenolic compounds in Omija-cheong were 24.1 mg-GAE/L and 1,635 mg-QE/L at 57 days of incubation, which were more than 9 and 5 times higher than those in Omija fruits, respectively. From the quality characteristics in processing Omija-cheong by low-temperature incubation, more than 60 days of incubation is required for the constant quality and value-added beverage.

• Korean Journal of Organic Agriculture
• /
• v.19 no.3
• /
• pp.405-416
• /
• 2011

Organic materials, such as chitin incubated solution (CIS) combined with neem oil (NO), nano silver silica (NSS), and Bordeaux mixture (BDM), were applied with and without agricultural chemicals (AC) (insecticide and fungicide) to investigate scab and mealybug occurrences and fruit qualities on 'Niitaka' pear trees in orchards in 2006. Fruits and leaves grown under CIS+NO without AC had less than 30%, scab occurrence, but CIS+NSS or CIS+BDM without AC had higher scab occurrence. Organic materials with AC decreased the scab to less than 20%. All treatments decreased mealybug occurrences to less than 10%, except for the fruits grown under CIS+BDM without AC. Fruit qualities varied among the treatments. Hunter value a, representing for the redness degree, was higher for fruits treated with CIS+NSS and CIS+BDM without AC than those with AC. Fruits treated with organic materials without AC had greater total phenolic and flavonoid compounds as well as antioxidant capacity in flesh and greater total phenolc compounds and antioxidant capacity in peel than those treated with the AC.

• Korean Journal of Agricultural Science
• /
• v.16 no.2
• /
• pp.225-238
• /
• 1989

These studies were conducted to investigate the extraction, purification and characterization of oriental pear (Niitaka. Pyrus pyrifolia Nak.) protease, and the results obtained were as follows: 1. Oriental pear protease was effectively extracted by the method of homogenizing pear pulp with 0.7 volume of 0.1M-sodium phosphate buffer, pH 6.5 containing 5mM-cysteine, 40mM-2-mercaptoethanol and 2mM-EDTA at 10,000 rpm for 5 min. 2. The protease was purified by ammonium sulfate fractionation, Sephadex G-100 filtration and DEAE-Sephadex A-50 column chromatography, and the purified enzyme gave a single protein band on polyacrylamide gel electrophoresis. 3. The specific activity of purified enzyme was 29.65 unit/mg protein and the yield was 7.22%. 4. The moecular weight of the protease was estimated to be about 51,000 by SDS-polyacrylamide gel electrophoresis, and the enzyme had Km value of 54.5 mg/ml for casein. 5. The purified enzyme had a maximum activity at pH 6.0 and $50^{\circ}C$, and was stable from pH 5.5-6.5 and at temperatures below $50^{\circ}C$ 6. Casein was a better substrate for this protease compared to hemoglobin. 7. The enzyme activity was markedly inhibited by p-chloromercuribenzoic acid and heavy metal salts such as $HgCl_2$ and $MnSO_4$ also considerably inhibited the enzyme activity.

• Korean Journal of Weed Science
• /
• v.11 no.2
• /
• pp.128-136
• /
• 1991

This experiment was conducted to evaluate the effect of plant growth regulators, dichlorprop and MCPB on the reduced effect of fruit drop and fruit quality before and after storage in apples. Dichlorprop was tested with dilution of 1000 at 30, 40, 50 days before harvesting, and MCPB with dilution of 4000 at 15, 25, 35 days before harvesting. The results are summarized as follows : Percentage of fruit drop was appeared to the notable reduction as compared with the untreated control when regulators was applied with dilution of 1000 at 30 days before harvesting by dichlorprop and with dilution of 4000 at 35 days before harvesting by MCPB. Degree of fruit colour showed to the remarkable promotion at all the treatment of 30, 40, 50 days before harvesting by dichlorprop as compared with the untreated control. Sugar contents in flesh was increased a little at the treatment of 30 days before harvesting by dichlorprop, but acid contents in flesh was reduced at all the treatment of 30, 40, 50 days before harvesting by dichloroprop and at 15, 25, 35 days before harvesting by MCPB. Passed firmness of fruit after storage was maintained at the treatment with dilution of 4000 at 35 days before harvesting. Therefore, it was repressed a softening of fruit, but by dichlorprop treatment at 30, 40, 50 days before harvesting, fruit firmmess was appeared to reduce according to the passage of storage period. Amount of ethylene evolution after storage was showed to reduce at all the treatment by early treated time of dichoroprop and MCPB, but carbon dioxide increased at treatment conditions such as the front. Accordingly, these relationship showed to be contrary each other.

• Korean Journal of Food Science and Technology
• /
• v.6 no.2
• /
• pp.98-108
• /
• 1974

Fourteen varieties of Korean chestnut were subjected to the test of adaptability for processing and the possibilities of colored products development. The fruit size of Korean chestnut ranged $11{\sim}18g$ and these small fruits could not be expected to be utilized for the processing of Marron glaces which requires larger size as $25{\sim}30g$. As the storge period is extended the discoloration tendency of chestnut flesh was increasingly developed, however, the fresh chestnut has shown layer-separating phenomenon and ragged surface of fruit which delivers disagreeable appearance to the finished product. The principal factors of discoloration occurred during processing were the behavior of tannin and darkening rate shown on flesh differed each other among varieties; the Chukpa and Yuma variety have exhibited the most serious discoloration and the Taab-b variety, the lightest. Taab-b variety in this connection could be expected to be available for Kanroni processing. For the industrialization of chestnut processing the flame-scorched peeling method is advisable. The capacity of this method is proportional to the square of scorching radius and highly flexible in controlling its performance. As for the processing of colored product, the sugar dehydration and coating and the sugar penetration method demand further study in basical views; however, the canned product of chestnut-redbean has shown the possibility of being utilized as a substitute for or paralleled use with the sugar-syruped canned product which so far has been considered as the only item of export to Japan.

• Horticultural Science & Technology
• /
• v.28 no.6
• /
• pp.996-1002
• /
• 2010

Flow cytometry (FCM) was used to measure the ploidy level of three different sports from 'Campbell Early' ($Vitis$ $labruscana$) grape. Results of the study showed different ploidy levels. FCM analysis for 'Campbell Early' grape which contains 2C DNA diploid cells showed single peak around 35-40 while 'Kyoho' grape with 4C DNA tetraploid cells had a different level of 70-80. However, analysis of the sports displayed a histogram with 2 peaks containing both 2C and 4C nuclei. There was no difference in histograms of 2C DNA flesh and pericarp; on the other hand, 4C DNA flesh type of sports had a different histogram from that of the 2C DNA pericarp. Chromosome numbers of diploid ('Campbell Early'), tetraploid ('Kyoho'), and three sports were counted under the microscope. 'Campbell Early' and 'Kyoho' have 38 and 76 chromosomes, respectively. Three different sports are mixoploids with mixtures of diploid and tetraploid cells. Microscopic observations of shoot apical meristems in sports from 'Campbell Early' grape were carried out to determine the type of plant chimera. 'Campbell Early' grape (diploid) and 'Kyoho' grape (tetraploid) showed that both had 2 tunica layers covering corpus cells, while the three different sports had tunica layers showing mostly oblique division. Most cells from 'Kyoho' grape were larger than 'Campbell Early' grape. Cells from L-2 and L-3 layers of the three sports were similar to 'Kyoho' grape in size, although all cells in L-1 surface layer were uniform in size like 'Campbell Early' grape. Results of FCM analysis indicated that both normal and polyploid cells could be intermixed in sports and could become mixoploidy consisting of diploid and tetraploid. All sports used in the tests were periclinal chimera plants with two distinct L-1 and L-2 cell layers. The result of this study suggests that all three sports which originated from 'Campbell Early' grape might be 2-4-4 type chimera formation.