• The Korea Journal of Herbology
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• v.23 no.4
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• pp.39-44
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• 2008

Objectives: Notopterygium incisum(N. incisum) and Saposhnikovia divaricata(S. divaricata) have been clinically used in traditional oriental medicine for treatment of inflammatory diseases. Also, a herbal mixture prepared with N. incisum and S. divaricata has been strongly linked to the anti-inflammatory effect. In this study, we evaluate the synergistic anti-inflammatory effect of N. incisum and S. divaricata. Methods: For evaluating the anti-inflammatory activity of a herbal mixture of N. incisum and S. divaricata in vivo, we measured the changed ear thickness in 12-O-tetradecanoyl-phorbol-13-acetate(TPA)-induced mouse ear edema model after topical application of herbal mixture. In addition, the levels of markers for inflammation, such as tumore necrosis factor (TNF)-${\alpha}$, interleukin (IL)-1${\beta}$, and nitric oxide(NO), were determined by ELISA assay in lipopolysaccharide-stimulated Raw 264.7 cells. Results: We reported that water extracts of N. incisum and S. divaricata combination significantly inhibited the mouse ear edema induced by TPA. Moreover, the water extracts of N. incisum and S. divaricata combination exhibited synergistic effects in down-regulating IL-1${\beta}$ level, but not TNF-${\alpha}$ and NO. Conclusions: These results suggest that combined treatment of N. incisum and S. divaricata, based on seven methods in prescription compatibility, has a synergistic effect in down-regulating inflammatory response both in vivo and in vitro models. Especially, it seems that IL-1${\beta}$ is a one of main target of the mixture of N. incisum and S. divaricata on anti-inflammatory activity.

• Herbal Formula Science
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• v.16 no.2
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• pp.133-144
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• 2008

Recently, people are becoming fatter in most parts of the world. Obesity is regarded as an important risk factor to adversely affect the health of humans, associated with hypertension, hyperlipidemia, diabetes, and cardiovascular diseases. In the present study, therefore, we tested the short time efficiency and safety of Bangpoongtongsungsan as a drug to decrease body weight in subjects with obesity. A total 48 volunteers (24 man, 24 woman) with body mass index (BMI : weight in kg divided by square of height in meters) of $23㎏/m^2$ participated in clinical study for 12 weeks, from 2008, March 01 to 2008, Jun 30. The subjects was received a dietary supplement of 12 pieces hard capsules per day (4 pieces in one, 3 times in a day) during the clinical study. At the end of clinical study, body weight of subjects was significantly decreased from $72.21{\pm}11.44\;kg$ to $70.53{\pm}11.67\;kg$ (p<0.05). Waist circumferences of subjects were reduced $91.58{\pm}6.43\;cm$ to $85.25{\pm}7.70\;cm$ (p<0.05). Also, a BMI of subjects was significantly decreased $26.07{\pm}2.35\;kg/m^2$ to $25.63{\pm}2.63\;kg/m^2$ (p<0.05). Particularly, a decrease in body weight, waist circumferences, and BMI was more significant in woman than man. But, other biochemical levels including lipid and glucose in plasma had no changes at the end of clinical study. In conclusion, administration of Bangpoongtongsungsan in subjects with high body weight fat could inhibit obesity associated with a decrease in body weight, waist circumferences, and BMI.

• The Journal of Pediatrics of Korean Medicine
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• v.20 no.2
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• pp.177-196
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• 2006

Objective : This experimental study was designed to determine the effects of BPT on obesity in vivo and in vitro. Methods : in vitro, BPTn extracts of various concentration(50, 100, 200 ${\mu}g/m{\ell}$) were added in 3T3-L1 cell. Adipocyte differentiation was measured by Oil Red O staining and Morphological examination. The expression of $C/EBP{\alpha}$ and $PPAR{\gamma}$ receptor was measured by western blot assay and RT-PCR in vivo, Rats were orally administered BPT daily for consecutive four weeks before poloxamer-407 induced hyperlipidemic state. The rats were sacrificed 24 hrs later for poloxamer-407 treated and then serum triglyceride, total cholesterol were measured ; Rats were orally administered BPT daily for consecutive four weeks before triton WR-1339 induced hyperlipidemic state. The rats were sacrificed 40 hrs later for triton WR-1339 treated and then serum triglyceride, total cholesterol were measured ; Rats with obesity were induced by the high fat-diet for six weeks and then serum triglyceride, total cholesterol, LDL-cholesterol, triglyceride, HDL-cholesterol, hydroxy radical, superoxide dismuatse activity were measured. Results : In vitro, The 3T3-L1 cells' differentiation was significantly decreased by BPT. The expression of $C/EBP{\alpha}$ and $C/EBP{\beta}$ was decreased by BPT. In vivo, BPT significantly reduced serum triglyceride, total cholesterol contents in poloxamer-407 treated rat. BPT significantly reduced serum triglyceride contents in Triton WR-1339 treated rat. Total cholesterol also reduced but did not show a significant change. BPT significantly reduced body weight gain of rat and adipose tissue mass of rats and serum triglyceride, LDL-cholesterol contents and significantly increased HDL-cholesterol, HTR(HDL-cholesterol/Total-cholesterol) in rats with obesity induced by the high fat-diet. BPT reduced blood lipid peroxide, hydroxy radical and increased superoxide dismuatse(SOD) activity.

• Journal of Korean Medicine Rehabilitation
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• v.21 no.2
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• pp.265-278
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• 2011

Objectives : This trial were conducted to evaluate the efficacy and adverse events of Bofutsusho-san(BTS) and Boiogiot-tang(BOT) by oriental obesity pattern identification on obese subjects, as compared to placebo. Methods : 166 subjects(body mass index ${\geq}25kg/m^2$) were recruited and randomized to receive BT(n=55), FH(n=55) or placebo(n=56) for 8 weeks. Anthropometric factors, serum lipid, glucose, blood pressure(BP), pulse rate, resting metabolic rate and oriental obesity pattern identification questionnaire were measured at baseline and 8 weeks. Adverse events and safety outcome variables were also checked during trials. Results : The frequency of top-scored oriental obesity pattern was ordered by indigestion(食積) > stagnation of the liver qi(肝鬱) > yang deficiency(陽虛) > spleen deficiency(脾虛) > phlegm(痰飮) > blood stasis(瘀血) in subjects. BTS group significantly decreased body weight, body mass index(BMI), waist circumference(WC), body fat mass, total cholesterol and HDL-cholesterol in stagnation of the liver qi(肝鬱) and WC in indigestion(食積). BOT group showed significant decrease of body weight, BMI, WC, and body fat mass in indigestion(食積) not in deficiency(虛症). Adverse events were reported most frequently in yang deficiency(陽虛) by BT group and stagnation of the liver qi(肝鬱) by BOT group. Conclusions : Bofu-tsusho-san was effective in treating obesity with stagnation of the liver qi(肝鬱). Obesity pattern identification could be a useful diagnostic tool predicting treatment effects and adverse events.

• Korean Journal of Acupuncture
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• v.22 no.3
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• pp.83-104
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• 2005

Objectives : DaeBangPungTang(DBPT) is one of the prescriptions used for the treatment of knee arthritis in oriental medicine. The present study aimed to examine the analgesic effect of DBPT on a rat model of carrageenan-induced arthritis, and the relations between DBPT-induced analgesia and endogenous nitric oxide(NO) and inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), and c-Fos protein expression in the spinal cord. Methods : Carrageenan-induced arthritis rat model was used to test the effect of DBPT as a chronic pain model. After the induction of arthritis, rats subsequently showed a reduced stepping force of the affected limb for at least tile next 4 days. The reduced stepping force of the limb was presumably due to a painful knee, since oral infection of indomethacin produced temporary improvement of weight bearing. DBPT dissolved in normal saline was minted several acupoints. After the treatment, behavioral tests measuring stepping force were periodically conducted during the next 12 hours. Results : DBPT produced significant improvement of stepping force of the hindlimb affected by the arthritis lasting at least 9 hours. The magnitude of this improvement was equivalent to that obtained after an oral injection of 3mg/kg of indomethacin and this improvement of stepping force was interpreted as an analgesic effect. DBPT produced the improvement of stepping force of the affected hindlimb in a dose-dependent manner. Both NO production and iNOS, COX-2 protein expression increased by arthritis were suppressed by DBPT. DBPT on combination with electroacupuncture (EA) produced more powerful and longer lasting improvement of stepping force of the hindlimb affected by the arthritis than either DBPT or EA did. Conclusion : The present study suggest that DBPT produces a potent analgesic effect on the chronic hee arthritis pain model in the rat and that DBPT-induced analgesia modulate endogenous NO through the suppression of iNOS/COX-2 protein expression.

• Korean Journal of Plant Taxonomy
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• v.41 no.4
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• pp.398-414
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• 2011

The flora in the region of Mt. Sobaeksan National Park ($N36^{\circ}50^{\prime}-37^{\circ}50^{\prime}$, $E128^{\circ}20^{\prime}-128^{\circ}43^{\prime}$) was surveyed from April of 2005 to October of 2011. The vascular plants from 27 field surveys were revealed to total 869 taxa, comprising 112 families, 429 genera, 756 species, four subspecies, 91 varieties and 18 forms. Sixty-eight taxa were the first records from this region. This field study discovered significant plants in various categories. Korean endemic plants numbered 25 taxa, and 18 taxa designated by the Korean Forest Service as rare plants were investigated in this region. The taxa in category II of rare and endangered plants and higher than the third degree among the floristic regional indicator plants designated by the Korean Ministry of the Environment were three taxa and 75 taxa, respectively. From a geographical perspective, limited distribution of Saxifraga octopetala, Corydalis grandicalyx, Aegopodium alpestre, and Polygonatum acuminatifolium at a latitude higher than that of Sobaeksan National Park suggest that Sobaeksan National Park might be the limit line of the distribution of the taxa. Stewartia koreana demonstrate a reversed pattern from the taxa above, occurring at a latitude lower than that of Sobaeksan National Park. In addition, naturalized plants of 53 taxa were recorded.

• Korean journal of applied entomology
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• v.31 no.4
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• pp.396-415
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• 1992

To investigate identification of species distribution state, population density of plant parasitic nematodes and damaged state by parasitism of nematodes with soil samples taken from major cultivating area of medicinal herbs in Kyungbuk districts are summarized as follow; total species belonging to genera in families were identified from 43 different medicinal herbs. The dominant nematode species on medicinal herbs was Meloidogyne hapla by 55.6% of field infection. Damaged state by parasitizm of Root-knot nematode, M. hapla, M. incognita are severed 15 medical plant except P. japonica, infected field were in 54.5~88.0%, and highered in population densities of 2nd larvae, Gall, Egg sac. Percentage of yield-decrease were 57.8% in A. gigas, 49.1% in P.japonica. Lighten effect of M. spp. by cropping system were lowered in population density in Rice+Paeony than Paeony+Paeony in paddy field, and also lowered in Sesame+Paeony or one year fallowing after harvest Paeony than Red pepper+Pseony, Paeony+Paeony in upland field.

• Journal of the Korean Society of Food Science and Nutrition
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• v.18 no.3
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• pp.321-327
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• 1989

The buffer activities of 72 species of natural pH adjusters in Chinese herb medicine materials of food materials by 100% acetic acid$({\mu}{\ell})$ titration until pH 4.0 in the $100m{\ell}$ of 1 % solution of the pH adjuster were evaluated for their abilities to retard acidification of Kimchi. There were 14 species above 100 of buffer activity such as Glycerehizae Radix(150), Siler divaricatum Benthan et Hooker etc. among selected Chinese herb medicine materials. In selected Chinese herb medicine materials, the buffer activities of Erillae Herba+Glycyrehizae Radix, Erillae Herba+Dioscoreae Rhizoma, Erillae Herba+Glycyrehizae Radix, Erillae Herba+Dioscoreae Rhizoma, Erillae Herba+Puerariae Radix, Chrysanthenum sinense+Puerariae Radix, Alismatis Rhizoma+Angelicae gigantis and Dioscoreae Rhizoma+Puerariae Radix were above 150 by same amount blending each other. Species and buffer activities of natural pH adjusters, which are higher buffer capabilities in mushroom, bean and vegetable classes were mungbean(207). soybean(160), pease(190), fungus(225), Garland chrysanthemum(200), amaranth(175), wild sesame leaf(200), parsley(202) and green pepper(257) etc. Species and buffer activities of higher buffer capabilities of natural pH adjusters of natural blending pH adjusters which is synergistic effect or natural blending pH adjusters which is synergistic effect mixing with radish juice were mung bean+peas(272), mung bean+soy bean(282), fungus+parsley(302), Garlan chrysanthenum+amaranth(300), Garland chrysanthenum+wild sesame leaf(277), amaranth+green pepper(300) and amaranthe+parsley(280) etc and those mixing with cabbage juice were peas+fungus(202), Garland chrysanthenum(207) and fungus+parsely (205) etc.

• Korean Journal of Environment and Ecology
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• v.26 no.4
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• pp.576-587
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• 2012

As being actual physiognomical vegetation on Daegwang sand dune in Imjado, the widest area is occupied by Pinus thunbergii community planted as windbreak forest whereas those communities such as Robinia pseudoacacia community, Elymus mollis community, Imperata cylindrica var. koenigii - Elymus mollis community, Carex kobomugi community, Ischaemum antephoroides community, Imperata cylindrica var. koenigii community, Phragmites communis community, Imperata cylindrica var. koenigii - Calamagrostis epigeios community occupy as band shape or patch. According to the result of the data collected and analyzed based on phytosociological method regarding 74 plots of survey area, the species composition of Daegwang sand dune vegetation is classified total 10 vegetation units comprises 7 communities and 5 groups. The 7 communities are classified into Pinus thunbergii community, Robinia pseudoacacia community, Rosa rugosa var. rugosa community, Ischaemum antephoroides community, Carex kobomugi community, Calamagrostis epigeios community, and the sub-units of Pinus thunbergii communities are classified into 3 groups of Pteridium aquilinum var. latiusculum group, Elymus mollis group, Pinus thunbergii topical group and the sub-units of Calamagrostis epigeios communities are classified into 2 groups of Phragmites communis group, Calamagrostis epigeios tipical group. The zonation of vegetation from coastal line indicated with the order of Elymus mollis - Carex kobomugi, Carex pumila, Lathyrus japonicus - Calystegia soldanella - Vitex rotundifolia, Lathyrus japonicus, Ischaemum antephoroides - Rosa rugosa var. rugosa. According to the analysis result of longitudinal section, it was found to be those types with wide width herbaceous vegetation of foredune, smooth slope of foredune, lots of dune ridges with no structure were less vulnerable to erosion of sand dune and advantageous to its recovery.

• Korean Journal of Environment and Ecology
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• v.23 no.4
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• pp.365-375
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• 2009

The growth characteristics of Pinus thunbergii windbreak forest around Goosipo Beach, Sangha-Myon, Gochang-Gun, Jeollabuk-Do have been investigated, and the results are summarized as follows. The soil in the site was light acid sand with pH 5.7, and the mean sodium concentration of soils was 1.42dS/m. The tree diameter, height, crown width, and shape ratio(Height/DBH) were better with tree density was coming lower. Especially, the rate of shape ratio under 60 was more in sites with low tree density. This demonstrated that the trees have grown more soundly when the tree density was lower. The direction of main winds and distribution ratio of tree inclination had a correlation. The inclined trees are seemed to be influenced by main winds when they were younger and by Typhoon partly. Eighty one vascular plant species were found in the sites, and more number of herbaceous plants (57 taxa) lived there than woody plants (24 taxa). And more plant species of dune were found at the site where dune have developed well. Some thinning works need at high tree density areas in order to ensure effects of coastal windbreak.