• Proceedings of the Korean Society for Applied Microbiology Conference
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• 1977.10a
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• pp.201.1-201
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• 1977

우리나라에서 대표적 정미성 조미류인 MSG가 발효법으로 산된 것은 1962년 이후부터이며 그 후 생산량도 많이 늘고 그 품질도 우수하여 현재 각국으로 수출하고 있는 실정이다. 특히 금년(1977년)에 들어와서 조미료의 왕자라 할 수 있는 핵광계조미료가 우리나라 주술진에 의하여 개발되고 곧 생산하게 되었음은 우리나라 발효공업을 국제적으로 과시한 일이라 하겠다.(중략)

• Journal of Life Science
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• v.28 no.7
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• pp.827-834
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• 2018

In this study, the biological activities of aqueous, ethanol, and methanol extracts of larvae of the edible insect Protaetia brevitarsis seulensis, fermented using several kinds of microorganisms, were tested in in vitro experimental models. Six effective microorganisms were used for fermentation, namely Lactobacillus plantarum JBMI F3, Lactobacillus plantarum JBMI F5, Lactobacillus gasseri Ba9, Aspergillus kawachii KCCM 32819, Saccharomyces cerevisiae KACC 93023, and Bacillus subtilis KACC 91157. Biological activities (${\alpha},{\alpha}^{\prime}-diphenyl-{\beta}-picrylhydrazyl$ [DPPH] free radical scavenging activity, reducing power, and fibrinolytic activity), and biochemical properties (phenolic compounds and flavonoids) were examined in aqueous, ethanol, and methanol extracts from P. brevitarsis seulensis powder and fermented P. brevitarsis seulensis powder. The total phenolic compounds and flavonoid contents were highest in the aqueous extract of B. subtilis-fermented P. brevitarsis seulensis powder. DPPH radical scavenging activity and reducing power were stronger in the fermented group than the nonfermented group. Fibrinolytic activity were highest in the extract from B. subtilis-fermented P. brevitarsis seulensis powder. The ${\alpha}-amylase$ activity in starch was higher in the fermented group than the nonfermented group, but there was no significant difference. These results provide basic data to understand the biological activities of bioactive materials derived from fermented P. brevitarsis seulensis larvae for the development of functional foods.

• 식품문화 한맛한얼
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• v.1 no.1
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• pp.9-12
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• 2008

발효는 어떤 식품에 유용한 미생물이 자라 큰 분자의 영양소를 분해하여 소화되기 쉬운 식품으로 변하게 한다. 그리고 음식속의 영양소를 이용하여 산성분, 맛성분, 생리활성 물질을 만들어내어 음식물의 보존기간을 연장할 뿐 아니라 균자체(유산균, 프로바이오틱 이라함)나 그 생성물들은 사람의 건강을 증진시키는 효과를 갖는다. 우리나라의 발효식품에는 김치, 장류(된장, 간장, 청국장, 고추장 등), 젓갈류, 막걸리가 대표적이다. 서양에서는 우유 발효식품으로 요구르트, 치즈, 채소발효식품으로는 사우어 크라우트, 피클, 올리브가 있고, 발효빵, 발효소세지, 술로는 포도주, 맥주 등이 있다. 발효 콩제품으로는 템페(인도네시아), 미소(일본된장), 쇼유, 낫또, 수후, 도우치 등 다양하다. 이에 우리나라에서 가장 많이 섭취하고 있는 김치, 된장 및 청국장의 암 예방효과에 대하여 알아보고자 한다.

• Bioindustry News
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• v.10 no.1
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• pp.15-22
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• 1997

• Proceedings of the Microbiological Society of Korea Conference
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• 1991.04a
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• pp.148-156
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• 1991

• Microbiology and Biotechnology Letters
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• v.10 no.2
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• pp.155-162
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• 1982

A cell-recycled continuous fermentation process was studied to produce ethanol from molasses using Sacchoromyces uvarum ATCC26602 at 35 $^{\circ}C$. The fermentation system was divided into two stages in order to reduce the inhibitions of ethanol and substrate for cell growth and fermentation rate. The first reactor was aerated at the rate of 0.12 vvm whilst the second was kept anaerobic. In medium composition studies, it was revealed that inorganic nutrient supplement to the diluted molasses with 14% fermentable sugar was not needed for the fermentation, however, phosphate limitation was observed when cell propagation was contemplated. By using the cell-recycled continuous fermentation system, 14.5 hour was required to produce 8.4-9.0% (v/v) of ethanol from diluted molasses containing 14% of fermentable sugar. The ethanol productivity was 6.2g/$\ell$hr with the yield of 88.1-94.4% to the theoretical value.

• Journal of The Korean Society of Grassland and Forage Science
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• v.44 no.2
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• pp.113-117
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• 2024

This study was conducted to find a way to improve quality by observing changes in quality and microbial communities according to whether corn silage was treated with additives and the storage period, and to utilize them as basic research results. The experimental design was performed by 2×4 factor desigh, and the untreated (CON), and the additive inoculated (ADD) silage were stored and fermented for 30 (TH), 60 (ST), 90 (NT), and 120 (OHT) days, with each condition repeated 3 times. There was no change in the nutrient content of corn silage according to additive treatment and storage period (p>0.05). However, the change in DM and the increase in the relative proportions of lactic acid content and Lactobacillales according to the storage period (p<0.05) indicate that continuous fermentation progressed until OHT days of fermentation. Enterobacterales (33.0%), Flavobacteriales (14.4%), Sphingobacteriales (12.7%), Burkholderiales (9.28%) and Pseudomonadales (6.18%) dominated before fermentation of corn silage, but after fermentation, the diversity of microorganisms decreased sharply due to the dominance of Lactobacillales (69.4%) and Bacillales (11.5%), Eubacteriales (7.59%). Therefore, silage maintained good fermentation quality with or without microbial additives throughout all fermentation periods, but considering the persistence of fermentation even in long-term storage and the aerobic stability, it would be advantageous to use microbial additives.

• Microbiology and Biotechnology Letters
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• v.18 no.4
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• pp.411-416
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• 1990

Microbiological conversion of sterols to 17-ketosteroids has been recongnized as a source for commerical preparation of steroidal drugs. For the purpose of strain development, we isolated microorganisms through enrichment culture method and identified an isolate strain. The strain was closely related to Brevibacterium ergthrogenes. The optimal conditions for 1, 4-androstadiene-3, 17-dione (ADD) formation were as follows; pH 7.4, lactose 0.2%, beef extract 0.2%, bentonite 0.5% in the chlolesterol fermentation medium. Maximum production was obtained with the addition of $\alpha$, $\alpha$'-dipyridyl (1 mM, final conc.) at 17-20 hours after incubation.

• Journal of Life Science
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• v.31 no.12
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• pp.1079-1087
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• 2021

To analyze gut microbiota of livestock in Korea and compare taxonomic differences, we conducted 16S rRNA metagenomic analysis through next-generation sequencing. Fecal samples from broiler chickens, pigs, and cattle were collected from domestic feedlots randomly. α-diversity results showed that significant differences in estimated species richness estimates (Chao1 and ACE, Abundance-based coverage estimators) and species richness index (OUTs, Operational taxonomic units) were identified among the three groups. However, NPShannon, Shannon, and Simpson indices revealed that abundance and evenness of the species were statistically significant only for poultry (broiler chickens) and mammals (pigs and cattle). Firmicutes was the most predominant phylum in the three groups of fecal samples. Linear discriminant (LDA) effect size (LEfSe) analysis was conducted to reveal the ranking order of abundant taxa in each of the fecal samples. A size-effect over 2.0 on the logarithmic LDA score was used as a discriminative functional biomarker. As shown by the fecal analysis at the genus level, broiler chickens were characterized by the presence of Weissella and Lactobacillus, as well as pigs were characterized by the presence of provetella and cattele were characterized by the presence of Acinetobacter. A permutational multivariate analysis of variance (PERMANOVA) showed that differences of microbial clusters among three groups were significant at the confidence level. (p=0.001). This study provides basic data that could be useful in future research on microorganisms associated with performance growth, as well as in studies on the livestock gut microbiome to increase productivity in the domestic livestock industry.

• Korean Journal of Food Science and Technology
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• v.19 no.6
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• pp.520-527
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• 1987

This study was attempted to identify microorganisms in fermenting soybean curd residues (SCR). The changes in contents of free amino acids, nucleosides, reducing sugars, and oligosaccharides were also studied. The fermentation of SCR which was by change inoculation was carried out at $55^{\circ}C$ for 48 hrs. pH increased gradually during fermentation and isolated microorganisms were identified as Bacillus subtilis and Bacillus licheniformis. Moisture content decreased from 80.8% to 58.4% at 48 hrs of fermentation and other proximate composition did not show any changes during fermentation. The content of total free amino acids increased rapidly and the number and quantities of each amino acid analyzed also increased during the course of fermentation. Glutamic acid, phenylalaine, lysine and aspartic acid were rapidly liberated during fermentation. As to the changes of nucleotides, 5'-AMP little changed during the first 36 hrs but subsequently decreased to approximately 1/6 after 48 hrs of fermentation. On the other hand, 5'-IMP plus 5'-GMP did not show almost any change during the first 36 hrs but increased about 3.5 times at 48 hrs of fermentation. However, 5'-XMP was not detected. The reducing sugar level showed rapid and steady increase throughout the fermentation and that of stachyose plus raffinose decreased slightly. From these results, a possible way of utilization of fermented SCR was proposed as a substitute for soybean in meju preparation.