• Proceedings of the Plant Resources Society of Korea Conference
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• 2012.05a
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• pp.7-7
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• 2012

인삼(Panax ginseng C.A. Meyer)은 두릅나무과(Araliaceae)에 속하는 식물로 동아시아에서 2,000여년 전부터 보기(補氣)제로 사용된 중요한 약재이다. 홍삼은 인삼의 뿌리를 찐 것으로, 고려시대 이전부터 쪄서 말리는 가공법이 있었던 것으로 알려져 있다. 건강기능식품에서 말하는 홍삼제품은 홍삼을 원재료로 하여 이를 분말화하거나 물이나 주정으로 추출하여 농축 또는 발효하여 식용에 적합하며, 진세노사이드Rg1과 Rb1의 합이 0.8~34 mg/g이 되어야 한다. 2011년 한국의 건강기능식품 시장은 약 3조 6,000억원에 이르며, 홍삼시장은 1조 2,000억원에 이른다. 2007년 6,420억원이었던 홍삼시장이 5년만에 두배가 된 것이다. 홍삼시장이 성장하면서 한국인삼공사(정관장)와 농협중앙회(NH한삼인), 동원F&B(천지인), 웅진식품(다채움수) 등의 회사가 기초연구와 다양한 제품개발을 통하여 경쟁하고 있다. 한국인삼공사의 경우, 최근 식품의약품안전청으로부터 홍삼의 '항산화' 기능에 대하여 건강기능식품기능성원료 인정을 받았고, '홍삼정타브렛'을 출시하였으며 '홍이장군' 브랜드를 리뉴얼하였다. 농협중앙회에서는 이미지 향상을 위한 '지성이면 한삼인' 시리즈와 짜 먹는 '뽀로로 홍삼 젤리'를 출시하였고, 동원F&B은 '홍송조화건조'를 제조공정에 도입하였고, 캡슐형태의 제품을 출시하였으며, 최근에는 꼬마버스 타요를 활용하여 '천지인 꼬마버스 타요' 시리즈를 개발하였다. 웅진식품, 대상웰라이프 등에서도 사포닌이 흡수되기 좋게 만든 '발효홍삼' 제품을 출시하고 있다. 향후 홍삼시장의 지속적인 성장을 위해 기초연구와 소비자의 니즈에 맞는 국내시장만이 아닌 해외시장까지 나아갈 수 있는 특화된 제품 개발이 절실히 요구되는 시점이다.

• KSBB Journal
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• v.8 no.2
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• pp.178-184
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• 1993

Extractive fermentations with the extract of Jerusalem artichoke in an aqueous-two-phase-system of polyethyleneglycol(PEG) and dextran(Dx) were investigated to obtain the effects of composition of PEG and Dx on both fermentation ,characteristics and partition ratio of alcohol. The specific growth rate of K. Fraglis CBS 1555 increased with a decrease of concentration of PEG and Dx. It augmented along with concentration of initial sugar up to 80g/l but decreased thereafter. The specific production rate of alcohol showed a rising tendency up to 100g/lof initial sugar, whereafter represented a decreasing trend. The partition ratio of alcohol between two phases augmented according to decrease of Dx comic. and increase of PEG cone. regardless of initial sugar concentrations. The ratio, however, decreased with Increment of initial sugar concentration at constant composition of PEG and Dx. The partition coefficient of alcohol had an ascending effect to the increase of PEG cone, but it had little effect on the changes of concentrations of Dx and initial sugar. The present study suggests that the optimum composition of PEG and Dx in the aqueous-two-phase-system by extractive fermentation were around 6.5%(w/v) of PEG and 3%(w/v) of Dx in considerations of emulsion state, sedimentation and separation of two phases, alcohol partition ratio, and specific growth rate.

• Microbiology and Biotechnology Letters
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• v.17 no.5
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• pp.510-518
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• 1989

Most of orange peels are disposed from orange juice manufacturing process. Thus, our purpose is to utilize these orange peels as fermentation substrate. We have investigated culture conditions and factors influencing citric acid production by an isolated strain, Asp. niger. Citric acid production was much higher in semisolid culture than in submerged culture and the particle size of ground orange peels was favored at 20 mesh in semisolid culture. The optimal pH and temperature were 4.5-5.0 and 3$0^{\circ}C$ respectively and the temperature cycling at 35$^{\circ}C$ for 20 hrs durig exponential phase, 1$0^{\circ}C$ for 4 hrs and 3$0^{\circ}C$ during stationary phase showed higher citric acid production than did at fixed temperature, 3$0^{\circ}C$. The addition of NH$_4$NO$_3$0.2%, MgSO$_4$7$H_2O$ 0.1%, methanol 2.5%, ethanol 1.5%, to culture medium promoted citric acid production but the addition of trace metal ions as nutrients had not effect on the acid production in orange peel medium. Under the optimal culture conditions, maximum yield of citric acid was 80.4% in solid medium. Almost of all original components of citrus peel was consumed by Asp. niger during fermentation.

• The Korean Journal of Food And Nutrition
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• v.16 no.3
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• pp.203-208
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• 2003

In wine making, to perform yeast culture effectively that is the most important factor, 5 (Montrachet, Pasteur Champagne, Epernay II, prise de Mousse, Lalvin W15) representative active dry yeasts were selected. These are results about physicochemical properties. As a consequence of examining the survival rate in both 17% ethanol solution and 200ppm sulfite solution after 72 hours, Lalvin W15 strain was the highest among the 5 representative active dry yeasts. Moreover, in 1% citric acid solution, the survival rate of Pasteur Champagne after 72 hours was the highest one. As a result of experiment of red wine that is fermented by 5 different active dry yeasts, the highest one of total acidity was must of Lalvin W15. And then, the must of the Epernay II contains the highest ethanol content. Regarding of the content of organic acid, the wine that is fermented by Prise de mousse was the highest one and the order could be explained by Tartaric > Malic > Citric.

• Journal of the Korea Organic Resources Recycling Association
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• v.20 no.4
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• pp.86-96
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• 2012

Hydrogen gas production of anaerobic fermentative process from food waste as a substrate was 3.47 mg $H_2/g$ COD. The hydrogen production was little less than the synthetic wastewater with sucrose as a substrate (7.56 mg $H_2/g$ COD). The B/A ratios of the synthetic wastewater and food waste were 3.73 or 8.01 respectively. Butyric acid was more produced when hydrogen production was higher. Microbial community in the samples was analyzed as Escherichia sp., Klebsiella sp., Clostridium sp., Bacterium sp., and Enterobacter sp. Clostridium sp. was detected both samples but Klebsiella sp. was more active with fermentation process of the food waste. Taxonomic description shows that 60% of the microorganism was ${\gamma}-proteobacteria$ and Firmicute and Bacteria was 20% respectively.

• Microbiology and Biotechnology Letters
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• v.15 no.6
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• pp.430-435
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• 1987

A chitin-immobilized enzyme amyloglucosidase(AMG) and a bacterium Zymomonas mobilis were coentrapped in alginate gel beads. Ethanol production was performed in a packed bed column reactor in a simultaneous saccharification and fermentation(SSF) mode using liquefied sago starch as a substrate. It was found that this process eliminated product inhibition and reverse reaction of glucose enhancing the rate of saccharification and ethanol production. At a low dilution rate of D = 0.11 hr$^{-1}$, the steady-state ethanol concentration was 46.0g/$m\ell$ (96.8 % of theoretical yield). The maximum ethanol productivity was 17.7g/$m\ell$, h at D = 0.83 hr$^{-1}$ when the calculation was based on the total working volume. The continuous production of ethanol was maintained stably over 40 days without problems in this reactor system.

• Applied Chemistry for Engineering
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• v.21 no.3
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• pp.349-352
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• 2010

The production process of cellulosic ethanol from rice straw using acidic saccharification was studied in this experimental work. The hydration by ultrasonic energy and the acidic saccharification using 10~30 wt% of $H_2SO_4$ were performed as pretreatment processes. Also, 10~50 wt% of yeast for 3~6 days was used for fermentation process. The yield of cellulosic ethanol was decided in the fermentation process. The optimum pretreatment condition was 375W of ultrasonic power and 30 min of hydration time using 20 wt% of $H_2SO_4$ and 2 h of the acidic saccharification time. Finally, the optimum fermentation condition was at the condition of 30 wt% of yeast and 3 days of fermentation time.

• Microbiology and Biotechnology Letters
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• v.14 no.4
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• pp.305-310
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• 1986

To improve the starch fermentation ability of yeast, hybrids were introduced by protoplast fusion of S. cerevisiae and S. diastaticus. The protoplasts of parental auxotrophic cells were fused in the presence of 10 mM CaCl$_2$and 30% of polyethyleneglycol (M.W 4, 000). The frequencies of fusant formation varied depending upon the strains used and were 3.51$\times$10$^{-4}$ to 5.04$\times$10$^{-4}$ for the regenerated protoplasts. The strains capable of extensive starch hydrolysis produce only 10% to total fusants. The 4 strains were finally selected by the results of starch fermentation and genetic stability test. The DNA content and cell volume of the fusants were greater than those of the parental strains.

• Membrane Journal
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• v.7 no.2
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• pp.65-74
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• 1997

Pervaporation pilot tests for obtaining the anhydrous ethanol, which is an automobile fuel additive for reducing air pollution, were carried out in the production field of fermented ethanol by using a PVA composite membrane. In the pervaporation dehydration of the ethanol/water azeotropic mixture, the membrane performance is concluded to be enhanced with the heating temperature of feed. In the determination of the permeate condensation temperature from the viewpoint of energy cost, an Optimal temperature was found to be near $0{\circ}C$. The results on the dehydration of fermented ethanol were similar to those of synthetic ethanol, which indicates that the pervaporation performance is not influenced by impurities contained in the ethanol to be dehydrated. From a comparison of calculated energy needed in the system and measured value in the pilot test, it is confirmed that the latent heat for vaporization of permeant on the permeate side of membrane is supplied from the feed.

• Journal of Korean Society of Environmental Engineers
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• v.36 no.4
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• pp.265-270
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• 2014

Protease producing microorganisms were isolated from many kinds of food waste and fermented foods, which contains high amount and variable kinds of degraded substances. Several microorganisms were identified by 16S rRNA full sequencing analysis methods. The activity of protease was analyzed and identified in variable conditions for the application. For industrial use for biowaste treatment some proteases were isolated, identified and selected from microbial cells. And the tests were carried for the further use. The protein degrading activity at low temperature is useful for the treatment of organic waste, which contains much proteins. By the protein degradation process the organic waste can be utilized in variable fields, for example from feedstuff supplement to fertilizer for agriculture. Bacterial cells with protease activity at low temperature were isolated and identified. The optimal conditions for microbial cultivation and protease production were studied.