• Journal of Biosystems Engineering
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• v.33 no.4
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• pp.276-281
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• 2008

A flow injection analysis method (FIA), which analyzes sample conditions after injecting a sample and reagents into a continuous stream, are recognized as the most adequate analyzing method according to the increase of sampling frequency, the decrease of measuring time and the diversity of measuring targets. Specially, the FIA is considered to be used effectively for the control of a fermentation process to produce fermentation food and useful microbial production by activation of a fermentation industry for development of biological materials. In this study, a flow injection analysis sensor unit was developed for on-line monitoring of the fermentation process. The performance was verified by on-line measuring the concentration of glucose of the fermentation process of wine. The glucose concentrations of the samples were measured every 12 hours during the whole fermentation process and compared with those by a HPLC. The concentration relative errors of glucose on the fermentation process of wine showed below 30% within 72 hours and over 50% after the 72 hours. The sensor unit had potential to on-line monitoring of the fermentation process but some problems to overcome for an commercial application.

• KSBB Journal
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• v.4 no.3
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• pp.241-245
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• 1989

A carriersupported mycelial growth of Sreptomyces erythreus was applied to erythromycin fermentation sistem using celite as a support material. Hyphal growth through the pore matrices of the materials showed anchorages and provided a stable biofilm growth. When the phospate concentration was limited to 0.8g corn steep liquor/L(corresponding to 40mg KH2PO4/L), the specific production rate of erythromycin was increased from 557$\mu$g/g-cell.hr under unlimited condition to 2, 898 $\mu$g/g-cell.hr. A fluidized-bed bioreactor was operated for erythromycin production by a repeated fed-batch mode. The control of free mycelial concentration and the extension of production phase were considered important to maintain the reactor productivity at a desired level. The erythromycin production under phosphate-limited condition could be maintained for at least 600hrs.

• Journal of Korean Society of Environmental Engineers
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• v.36 no.10
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• pp.667-671
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• 2014

By analyzing seed germination indexes of cabbage and radish, this study aims to evaluate the toxicity of food waste compost, which is produced by fermentation-extinction technology with bio wood chips. In the experimental results, the seed germination index of food waste compost for cabbage and radish was shown in the range of a minimum 104 and maximum 170. It was satisfied with the over 70 criteria in the level of the Korea fertilizer process specification. Consequently, the food waste compost from fermentation-extinction reaction with bio wood chips was evaluated by the appropriate compost maturity for the plantation.

• KSBB Journal
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• v.19 no.2
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• pp.98-103
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• 2004

The aim of the present study was to investigate how the hardness of groundwater affects in the alcohol fermentation. Ozone plays an important role to enhance the water quality, resulting in 85% reduction of hardness, and 30% increase in total glucose produced due to increased conductivity and biodegradability of water. After all, experiments using ozone are presented for the improvement of alcohol productivity. Although initially increased slightly alcohol production, higher than expected ethanol production was observed, with ozone treatment resulting in 20% higher production.

• KSBB Journal
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• v.22 no.6
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• pp.401-408
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• 2007

It's not easy to detect the specific compounds from various compounds that fermented in bioreactor. The electronic nose was an instrument, which comprised of an array of electronic chemical sensors with partial specificity and an appropriate pattern recognition system, capable of recognizing simple or complex volatiles. It can conduct fast analysis and provide simple and straightforward results and is best suited for quality control and process monitoring in field of biotechnology. This review examined the application of electronic nose in biotechnology and brief explanation of its principle. In this minireview numbers of applications of an electronic nose in biotechnology include monitoring fermentation process, to overcome interference with alcohol, and to detect contaminant microorganism were discussed. The electronic nose would be useful for a wide variety of biotechnology when correlating analytical instrumental data with the obtained data from electronic nose.

• Korean Chemical Engineering Research
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• v.48 no.3
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• pp.342-349
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• 2010

Lactic acid is widely used in the food, chemical and pharmaceutical industries, and there is an increasing demand for lactic acid as the raw material of poly lactic acid(PLA), which is a biodegradable polymer. Lactic acid production has been changing over from production by synthesis to production by fermentation, since the fermentation process is more nature friendly and economic. However, the fermentation method generates excess water and impurities with high boilers. The presence of high boilers and non volatility of lactic acid makes the separation of lactic acid very difficult job. Also, the purification-separation process requires the many investment costs and energy costs. Reactive distillation concept was also introduced for the process, giving higher selectivity and yield compared to the convention method. We introduce a new highly integrated process, reactive diving wall column, to reduce the capital and energy cost for producing a pure lactic acid. The reactive dividing wall column combines reactive distillation and dividing wall column. We compared capital and energy consumption required for the purification of lactic acid the between the proposed design structures. And we examined the effect of major process variables on the process performance and determined optimal process.

• Korean Journal of Food Science and Technology
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• v.27 no.2
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• pp.176-180
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• 1995

Direct contact heat exchange (DCHE) method has been employed to investigate the separation of ethanol from dilute aqueous solutions. Bubbles at high temperature were dispersed into a continuous liquid phase, generating temperature gradient in air-liquid interface, which causes heat and mass transfer accordingly. The experiments were performed in the ranges of jet regime air flow. The air-water stripping coefficient increased $5{\sim}10,\;and\;1{\sim}1.5$ times as temperature and air flow rate increased, respectively. The recovery ratio based on the initial ethanol concentration reached into 80% at the air flow rate of 84.88 m/min. The initial ethanol concentration showed little effect on the stripping coefficient and the recovery ratio.

• Land and Housing Review
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• v.2 no.1
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• pp.87-92
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• 2011

The anaerobic digestion of food waste effluent through single- and two-phase process was estimated and compared in this study. The treatment efficiencies for total solid(TS), volatile solid(VS), tCOD(total COD) and sCOD(soluble COD) were invariably higher in the single-phase process, which was accounted for by the fact that the treatment efficiency of organic wastes usually showed an inverse relationship with organic loading rate in the anaerobic digestion. In fact, the organic loading rate was lower for single-phase process. The concentration of tCOD were significantly lower in two-phase process but much more biogas was produced, compared to single-phase anaerobic digestion process, which might be explained partly by the relatively higher stability of two-phase process resulting from the separation of acid phase from methane phase.

• Korean Chemical Engineering Research
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• v.53 no.4
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• pp.417-424
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• 2015

In this study, the pretreatment of Helianthus tuberosus residue had been performed. The two-stage pretreatment on flow-through process were applied in the interests of increase of sugar production yield on enzymatic saccharification. The delignification by aqueous ammonia and the fractionation of hemicellulose by sulfuric acid solution as pretreatment solution were confirmed for effects of enzymatic saccharification. Two-stage pretreatment process was performed using aqueous ammonia and sulfuric acid. The first step was performed with aqueous ammonia for 40 min at $163.2^{\circ}C$ and the second step was performed with sulfuric acid solution for 20 min at $169.7^{\circ}C$. And then, the first step was performed with sulfuric acid solution and the second step was pretreated with aqueous ammonia. At this time, the glucose production was 30.7 g and the glucose yield was 72.4% in the first step process with aqueous ammonia. And, the glucose production was 20.9 g and the glucose yield was 49.3% in the first step process with sulfuric acid solution.

• The Korean Journal of Food And Nutrition
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• v.19 no.4
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• pp.460-465
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• 2006

This study was conducted to investigate the isoflavone contents including daidzein and genistein in soy sauces made with whole grain soybean Meju(WGSM), comparing with defatted Meju(DM) du.ins fermentation. Total isoflavone contents in 2 soy sauces made with DM, Control and product A, increased with period of fermentation and were 15 and 4.3 times higher after 180 days of fermentation than on the day before fermentation respectively. Meanwhile, 2 soy sauces made with WGSM, product B and C, had the highest amounts with $20.53{\mu}g/g\;and\;19.52{\mu}g/g$ of isoflavones on the day before fermentation respectively, and then decreased with fermentation time. High amounts of isoflavones were detected in 20 days of fermentation of soy sauce which made with WGSM and wheat Meju(product D). Daidzein was the largest amounts in isoflavone contents, regardless of total or free isoflavone types. The level of daidzein increased with fermentation time in 2 soy sauces made with DM, Control and product A, whereas 2 soy sauces made with WGSM, product B and C decreased with fermentation time, instead of increasing of genistein contents.