• Journal of Energy Engineering
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• v.2 no.1
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• pp.123-132
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• 1993

This work is focussed on the reduction of ethanol separation energy from alcohol fermented broth and categorized into the development of a computer program for the design of the pressurized/depressurized distillation process which has been regarded as one of the energy-reducing models for the conventional distillation process, the optimization of operating conditions of distillation towers by means of the developed program, and the evaluation of the total annual energy cost of pressurized/depressurized distillation columns compared with that of the conventional single distillation columns. The operating pressures are, in case of pressurized/depressurized distillation, 3103/760 mmHg, 3103/450 mmHg, 3103/160 mmHg, and in case of conventional distillation, 760 mmHg. The optimum reflex rations which the sum of the annual energy cost and the annual fixed cost for each process becomes minimum are 3.7475/2.9111 for the operating pressures of 3103/760 mmHg, 3.814/2.9712 for 3103/450 mmHg, 3.0783/2.2400 for 3103/150 mmHg, and 3.8544 for the atmospheric operating pressure. And the annual energy cost of pressurized/depressurized distillation process for the above-mentioned operating pressures is distributed between 42% and 47% of that of conventional distillation process.

• Applied Chemistry for Engineering
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• v.20 no.4
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• pp.370-374
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• 2009

A double-layer optical sensing membrane has been fabricated to measure the concentration of dissolved oxygen (DO) and pH value simultaneously. (tris(4,7-diphenyl-1,10-phenanthroline (Rudpp) ruthenium(II)) as a DO sensitive dye has been mixed in the methyl trimethoxy silane (MTMS) sol-gel solution and coated onto one well in a 24-well microtiter plate. On the DO-sensing layer the GA (3-glycidoxypropyl trimethoxy silane (GPTMS), 3-aminopropyl trimethoxy silane (APTMS)) sol-gel solution mixed with 8-hydroxypyrene-1,3,6-trisulfonic acid trisodium salt (HPTS) has been coated and used to measure pH values. The double-layer sensing membrane was affected by ionic strength and temperature. The double-layer sensing membrane for DO and pH has been applied to online monitor in microorganism cultivation processes and showed a good performance.

• Microbiology and Biotechnology Letters
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• v.48 no.3
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• pp.344-357
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• 2020

Strain improvement and bioprocess development were undertaken to enhance hyaluronic acid(HA) production by Streptococcus zooepidemicus cells. Using a high-yielding mutant strain, statistical medium optimization was carried out in shake flask cultures, resulting in 52% increase in HA production (5.38 g/l) at the optimal medium composition relative to the parallel control cultures. For sufficient supply of dissolved oxygen (DO), which turned out to be crucial for enhanced production of HA, agitation system and speed were intensively investigated in 5 L bioreactor cultures. Increase in oxygen mass transfer coefficient (k_La) through increment of agitation speed (rpm) and 35% expansion of diameter of the newly-designed impellers showed significantly positive effects on HA production. By installing an expanded Rushton-turbine impeller for efficient break-down of sparged air, and an extended marine impeller above the Rushton-turbine impeller for efficient mixing of the air-born viscous fermentation broth, maximum amount of HA (9.79 g/l) was obtained at 450 rpm, 1.8 times higher level than that of the corresponding flask culture. Subsequently, the possibility of bioprocess scale-up to a 50 L bioreactor was investigated. Despite almost identical maximum HA production (9.11 vs 9.25 g/l), the average HA volumetric productivity (r_p) of the 50 L culture turned out only 74% compared to the corresponding 5 L culture during the exponential phase, possibly caused by shear damages imposed on the producing cells at the high stirring in the 50 L culture. The scale-up process could be successfully achieved if a scale-up criterion of constant oxygen mass transfer coefficient (k_La) is applied to the 50 L pilot-scale bioreactor system.

• Journal of the Korean Society of Food Science and Nutrition
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• v.45 no.4
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• pp.542-550
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• 2016

This study was carried out to investigate the optimal processing conditions for odor removal and maximal antioxidant effects of oyster (Crassostrea gigas) hydrolysate. The optimal hydrolysis conditions were 3.3% neutrase as the protease, $50^{\circ}C$ as the hydrolysis temperature, and 8.3 h as the hydrolysis time. Fish odor of enzymatic oyster hydrolysate was greatly reduced during Saccharomyces cerevisiae fermentation at $24^{\circ}C$ with 0.5% glucose. The protein content of the fermentation product from oyster hydrolysate powder was 25.7%, which contained the major amino acids Glu, Asp, Lys, Arg, Gly, and Ala, whereas Leu, Ala, Phe, Val, and Tau were abundant free amino acids. The important minor minerals were Zn and Fe. Toxicity against Chang cells was not observed in the fermentation product from the oyster hydrolysate up to $200{\mu}g/mL$. The results suggest that fermentation with S. cerevisiae could reduce the fish odor of enzymatic oyster hydrolysate. The hydrolysate has potential application as a food ingredients and nutraceutical.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.1
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• pp.438-444
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• 2015

The aim of this research was to apply a HACCP system (Hazard Analysis Critical Control Point) to fermented milk. The main ingredients of fermented milk, work facilities and workers were obtained from a company named YD, which is located in Seobuk-gu, Cheonan-si between November 5 2013 to April 13, 2014. A manufacturing process chart was prepared by referring to the manufacturing process of fermented milk manufacturers in common. The manufacturing process chart was made with raw materials; Raw milk, High Fructose Corn Syrup, Oligosaccharides, Lactic Acid bacteria and Subsidiary ingredients, Warehousing of packaging materials, Storage, Input, Preheating, Mixing, Homogeneity, Sterilization, Precooling, Culture, Filtration, In packaging, Out packaging, Storage, and Consignment, as listed Table 1. The results of the microbiological hazard analysis on the raw materials was safe after sterilization($90^{\circ}C{\pm}5^{\circ}C$, $35{\pm}3min.$) On the other hand,a microorganism test of an environment and workers suggested that the microbiological hazard should be reduced through systematic cleaning and disinfection accompanied by improved personal hygiene based on hygienic education on workers and the management of microorganisms in air.

• Journal of the Korea Organic Resources Recycling Association
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• v.16 no.2
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• pp.29-37
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• 2008

In this work, it was investigated that various aspects of process, application situation, merits and short-coming results of the thermophilic methane fermentation with highly concentrated organic waste substances such as sewage sludges, food wastes and excretions. The merits of this methane fermentation were that it had a very fast reaction rate and was possible to proceed in high loads. It was also high in mortality for pathogenic microorganism and the digested sludge was more hygienic. However, the short-comings were that more energy was required for heating in the fermentation facility, no surplus energy could be gained from low concentration of organic waste, the fermentation treatment dropped level of water quality, thus burdens discharging process of water. Especially, the high concentration of methane fermentation could possibly lack nutritious salt and could face the disturbance by ${NH_4}^+-N$, a proper alternative was required. In general, thermophilic methane fermentation was considered as a better mean in disposing of cow excretion and food waste which were highly concentrated organic wastes. On the other hand, under the condition where the concentration of waste material was low and the high concentrate waste material became higher than 3,000 mg/L in ${NH_4}^+-N$, thermophilic methane fermentation resulted less desirable outcome.

• Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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• 2003.04a
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• pp.148-148
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• 2003

최근 홍국색소는 천연계착색료로서 뿐만 아니라 발효식품, 발효조미료등에도 이용되어 기호에 맞는 새로운 홍국 개발도 적극적으로 진행되고 있어 인지도가 높아지고 있다. 혈청콜레스테롤저하작용, 혈압강하작용, 혈당강하작용, 항산화작용등 여러가지 약리효능을 지닌 홍국균을 이용해 재래적 방법에 근거하여 홍국주를 제조하여 그 제조공정을 확립하고 품질특성을 확인하였다. Monascus purpureus를 액체배양법에 의해서 홍국코지를 제조하고 코지의 역가는 $\alpha$-amylase와 $\beta$-amylase 활성은 400unit와 4.44unit를 나타났으며, acid protease 활성은 80unit, neutral protease 활성은 91.4unit로 나타났다. 홍국코지에 증자된 쌀과 물을 혼합하여 발효경과를 측정하고, 발효가 끝난 홍국주의 DPPH법에 의한 전자공여능, 아질산염 소거능, Total phenolic compound함량, ACE저해작용등을 측정하였다. 홍국주의 발효경과 분석은 알콜도수는 2차 담금 후 2일째-13.2도, 4일-13.5, 6일째-13.5, 8일째-13.5, 10일째-13.4로 나타났다. 환원당은 2일째-23.26, 4일-20.68, 6일째-21.74, 8일째-19.93, 10일째-14.23 $^{\circ}$Brix로 점점 감소한다는 것을 볼 수 있으며, 색도에서 적색을 나타태는 Hunter value a값은 2일째 -14.72, 4일-14.97, 6일째-15.70, 8일째-16.43, 10일째-17.29로 시간이 지날수록 붉은 색소가 증가한다는 것을 알 수 있었다. pH는 2일째-4.52, 4일-4.02, 6일째-4.11, 8일째-13.11, 10일째-13.18로 pH가 약간 낮아진 것을 볼 수 있다. 산가는 2일째-8.4, 4일-6.7, 6일째-7.0, 8일째-7.5, 10일째-11.5로 pH가 산성화 될 수록 산가는 증가하는 것을 볼 수 있다. 홍국주의 전자 공여능에 의한 항산화력은 25.6%, 아질산염 소거능은 27.6%, Total phenolic compound 함량은 12.34mg%, ACE저해작용은 38%의 항산화력을 나타냈다.

• KSBB Journal
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• v.10 no.2
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• pp.126-130
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• 1995

A membrane gas sensor was developed for the measurement of ethanol concentration during acetic acid fermentation. The fermentation broth including ethanol was permeated through the silicone membrane by synthetic air as a carrier gas and was detected by a semiconductor gas sensor. The optimum conditions of membrane gas sensor were 20m1/min of flow rate and 0.5mm of membrane thickness. In acetic acid fermentation, an on-line measurement of ethanol concentration was conducted by the proposed membrane gas sensor and then the on-line sensor signal, was compared with the result of off-line analysis by gas chromatography. As a result, a correlated response over the range of $0∼70g/\ell$ was shown between membrane gas sensor and gas chromatography and this use of membrane gas sensor was experimentally ascertained for the monitoring and control of bioprocess like acetic acid fermentation.

• KSBB Journal
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• v.8 no.2
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• pp.172-177
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• 1993

In order to reduce BOD of wastewater from alcohol distillery manufactory, the production of single cell lipid was attempted. Among five yeast strains tested, Rhodotorula glutinis was most desirable for lipid production. Wastewater was treated with 2N NaOH and used as a medium. The optimum pH and temperature for lipid production were found to be 5.0 and $30^{\circ}C$, respectively. The addition of monobasic phosphate was good for cultivation of Rhodotorula glutinis. The C/N ratio was an important factor for lipid production and composition. The best C/N ratio was 50 for the production of single cell lipid. By cultivation Rhodctorula glutinis for 4 days, 4g/L of single cell lipid was harvested and BOD of wastewater reduced by 88.7%.

• 한국생물공학회:학술대회논문집
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• 2005.04a
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• pp.272-276
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• 2005

Food wastewater derived from three-stages methane fermentation system showed high concentrations of sCOD, T-N and $NH_{3}-N$. To treat the organic wastewater, the optimal operating conditions for high efficiency $UV/TiO_{2}$ photocotalytic system have been investigated. In the first process, wastewater was pre-treated with $FeCl_{3}. The optimum pH and concentration for coagulation were 4.0 and 2000 mg/L, respectively. Through this process, 52.6% of $COD_{cr}$ was removed. The second process was $UV/TiO_{2}$ photocatalytic reaction. The optimum conditions for the operation of $UV/TiO_{2}$ photocatalytic system developed in this lab have been studied. In this process, CODcr was removed from 2890 to 184 mg/L and T-N was removed from 2496 to 914 for 24 hours, respectively.