• 농업과학연구
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• 제42권3호
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• pp.237-244
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• 2015

Four ruminally cannulated Holstein steers (BW $401.0{\pm}2.22kg$) fed TMR containing low protein (CP 9.63 %) as a basal diet were used to investigate the effects of cornell net carbohydrates and protein system (CNCPS) fraction enriched protein feeds on rumen fermentation and blood metabolites. The steers used in a $4{\times}4$ Latin square design consumed TMR only (control), TMR with rapeseed meal (AB1), TMR with soybean meal (B2) and TMR with perilla meal (B3C), respectively. The protein feeds were substituted for 30 % crude protein of TMR intake. For measuring ruminal pH, ammonia-N and volatile fatty acids (VFA), ruminal digesta was sampled through ruminal cannula at 1 h-interval after the afternoon feeding. Blood was sampled via the jugular vein after the ruminal digesta sampling. Different CNCPS fraction-enriched proteins did not affect (p>0.05) ruminal pH except B3C being numerically low compared with the other groups. Ammonia-N and VFA were not significantly different among the experimental groups. Numerically low ammonia-N appeared in the steers fed rapeseed meal even though it contained high soluble N composition (A and B1 fractions). The discrepancy is unclear; however this may be related to low protein level in the diet and/or low DM intake. Blood metabolites were not significantly affected by the protein substitution except for blood urea nitrogen that was significantly (p<0.05) increased.

• Journal of Animal Science and Technology
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• 제47권2호
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• pp.253-260
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• 2005

This study was conducted to utilize the wastes produced from thinning the forest as a roughage source for ruminants. Four ruminally cannulated Korean steers were used to investigate the ruminal fermentation characteristics and blood concentrations of stress-related hormones. Treatments were composed of the rice straw only (Control) and the 30% pine silage plus 70% rice straw(Pine silage) as roughages. The experiment was conducted with four replicates by a double tum over design. For sampling of blood under high temperature steers were accommodated in respiratory chamber. Concentrations of ruminal pH, volatile fatty acids and ammonia were not affected by substitution of pine silage. Mean concentration of blood thyroxine was significantly(P < 0.01) lower in the pine silage than the control. However, blood cortisol concentration was not affected by substitution of pine silage, even though it was significantly(P < 0.05) decreased after exposure for 6 hrs in high temperature. It was concluded that the pine silage using the wastes produced from thinning the forest could be use as a substitute roughage for reduction of heat stress in ruminant.

• 한국축산시설환경학회지
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• 제19권2호
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• pp.155-162
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• 2013

The objective of this study is to investigate the effects of Cynanchum wilfordii (CW) on cell viability, anti-oxidant activity, volatile fatty acid (VFA) production and methane gas production. Collected rumen fluid incubated with CW powder (1% w/v) for 12 and 24 hours were analyzed for pH, VFAs and methane. Alamar blue assay showed no significant difference on the viability of 3T3-L1 and C2C12 cells treated with CW for 24 hours. TBARS data showed a dose dependent increase on the antioxidant activity of CW. VFAs increased in the CW-treated groups compared to the control group. In addition, propionate increased more than other VFAs by the treatment with CW. There was a significant decrease in methane gas production in batch culture treated with CW in 12hrs. In conclusion, it was suggested that Cynanchum wilfordii could manipulate rumen fermentation considered by increasing VFA production and inhibition of methanogenesis.

• 한국유기농업학회지
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• 제25권2호
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• pp.461-473
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• 2017

Total mixed ration (TMR) including concentrate diet and roughage together have been used for the ruminant animal. Relatively high concentrations of moisture and water soluble carbohydrate are representative feature of TMR. Those moisture and water can also provide a niche for bacterial growth. Therefore, a possible fermentation of TMR induced by micro-organism is generally accepted. The present study hypothesized that different lactic acid bacteria could alter fermentation of TMR and subsequently rumen fermentation. Three lactic acid bacteria, Lactobacillus paracasei (A), L. plantarum (B) and L. parabuchneri (C), were employed and 7 treatments under full factorial design were compared with control without inoculation. TMR for dairy cow was used. Significant alterations by treatments were detected at lactic acid and butyric acid contents in TMR (p<0.05). Treatment AC (mixture of A and C) and BC (mixture of B and C) showed great lactate production. Great butyrate production was found at treatment C. At in vitro rumen fermentation, treatments B, C and AB (mixture of A and B) showed significantly great total gas production (p<0.05). All treatments except treatments B and AB, showed less dry matter digestibility, significantly (p<0.05). Total volatile fatty acid production at treatment AC was significantly greater than others (p<0.05). In individual volatile fatty acid production, treatment AB and AC showed great acetate and propionate productions, significantly (p<0.05). This study investigated correlation between organic acid production in TMR and rumen volatile fatty acid production. And it was found that butyric acid in TMR had significant negative correlation with acetate, propionate, total volatile fatty acid, AP ratio and dry matter digestibility.

• 한국축산시설환경학회지
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• 제21권3호
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• pp.113-122
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• 2015

An experiment was conducted to examine the effects of Kalopanax pictus Nakai (Kalopanax) on in vitro rumen fermentation and methane (CH4) reduction. Kalopanax trunk was extracted with 70% ethanol and 70% methanol. Rumen fluid, alfalfa hay and buffer (control: C) supplemented with 0.3% Kalopanx juice (T1), 0.3% ethanol extract (T2) and 0.3% methanol extract (T3) in the total volume of culture medium were incubated at $38^{\circ}C$ for 24h and 48h. Rumen pH was lower in all Kalopanax treatments during all incubations than that in control (p<0.05). Total VFA and total gas production in T2 and T3 was significantly higher than that in C at 48h incubation (p<0.05). Ammonia-N was decreased in all treatments compared with C during the incubation periods (p<0.05). At 24h incubation, $CH_4$ contents were significantly reduced by both alcohol extracts. It is concluded that supplementing Kalopanax extracts can stimulate ruminal fermentation of rumen microorganisms and inhibit methanogenesis.

• 한국초지조사료학회지
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• 제15권3호
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• pp.215-221
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• 1995

Three fistulated nonlactating Holstein cows were used to examine the change in ruminal characteristics when fed diets of 100% corn silage, 50% corn silage + 50% wet brewers' grain(WBG) and 100% WBG, and also twenty Holstein heifers averaging 156 $\PM$ 3.2kg BW were randomly assingned to determine the adequate feeding level of concentrate with the following treatments ; Only WBG, concentrate to 0.5, 1.0 and 1.5% of BW under feeding WBG ad libitum. Tne main results were as follows : I. Ruminal pH was minimized 3 hrs after feeding and then gradually increased. The mean ruminal pH was highest in feeding only WBG (6.72) and lowest in only corn silage(6.0) 2. Ruminal $NH_3$-N concentration was maximized 1 hr after feeding and then gradually decreased regardless of diets, n7e average ruminal $NH_3$-N content was highest in diet of 100% WBG(12.9 rngldl) and lowest in 100% corn silage(5.9 mg/dl) 3. Acetic acid percent among VFA was highest when WBG replaced 50% corn silage and acetic to propionic acid ratio was higher in order of feeding only corn silage, 50% corn silage + 50% WBG and only WBG. 4. Ruminal bacteria and protozoa number in diet of 50% corn silage + 50% WBG were 8.14 $\times$ > $10^5$ and 1.45 $\times$ $10^5$, respectively, which were most of all treatments. 5. Daily gain and feed efficiency of Holstein heifer in feeding concentrate to 0.5% of BW and forage hay to about 20% of total DM intake when fed free WBG were better

• 한국미생물·생명공학회지
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• 제42권3호
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• pp.211-218
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• 2014

본 연구에서는 동물유래 시료들로부터 양돈 사료첨가용 유산균을 개발하기 위하여 enterotoxigenic E. coli, Salmonella Typhimurium, Clostridium perfringens 등 양돈산업에서 심각한 문제를 일으키는 인수공통 병원성 세균들과 Clostridium jejuni, Listeria monocytogenes, Staphylococcus aureus와 같은 식중독균을 강하게 저해하는 Bifidobacterium 유산균을 분리하고자 하였다. 이를 위하여 소의 반추위 내용물, 닭 창자 내용물, 돼지 분변 등의 시료들로부터 총 65주의 혐기성미생물을 분리하였다. 이 중에서 항병원세균활성이 가장 높은 4주를 선별하였는데, 이들은 16S rDNA 염기서열 분석방법에 의하여 3주의 B. boum과 1주의 B. thermophilum으로 동정되었다. 특히 B. thermophilum는 분리주들 중에서 가장 높은 돼지 장 상피세포에 대한 부착성을 보였고, 여러 인수공통병원세균들과 식중독균들에 대한 높은 항균력, 산과 담즙내성을 보여 양돈용 생균제 후보균주로서 우수한 특성을 나타내었다.

• 한국유기농업학회지
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• 제28권4호
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• pp.605-614
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• 2020

This study was conducted to investigate the effects of TMR on in vitro rumen fermentation and methane production of goat with different forage sources. The experiment was arranged 4×2 factorial design. The different forage sources were rice straw (RS), Italian rye grass (IR), timothy (TI) and alfalfa (AL), respectively. There were two different forage : concentrate ratios such as 20:80 (20) and 50:50 (50), respectively. Therefore, totally 8 treatments were used: 1) RS20, 2) RS50, 3) IR20, 4) IR50, 5) TI20, 6) TI50, 7) AL20, and 8) AL50, respectively. The rumen fluid of goat was collected from the slaughterhouse. For fermentation parameters, ruminal pH, total gas, methane, hydrogen, ammonia nitrogen, and volatile fatty acid were determined. The pH values were within an optimal range across all treatments. Total gas productions at TI20 and AL50 were significantly greater than others (p<0.05). Methane production was significantly lower in TI and AL compared with other treatments (p<0.05). The relatively high dietary NDF content in treatments showed significantly lower methane production (p<0.05). Significant alterations treatments were detected at ammonia nitrogen concentration according to the ratio of forage : concentrate (p<0.05). AL treatment showed greater total volatile fatty acid production compared with other treatments (p<0.05). Therefore, the present study suggests that both Timothy and Alfalfa could be recommendable forage sources for goat based on results with volatile fatty acid as an energy source and methane as an index for energy loss and environmental issues. Also, the 50:50 (forage : concentrate) ratio would prefer to 20:80.

• 한국정보기술학회논문지
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• 제16권12호
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• pp.127-137
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• 2018

가축의 분만은 농가의 재산을 늘릴 수 있는 중요한 수단이므로 이를 관리하는 것은 농업 경영에 필수적인 항목이다. 특히 축우는 다른 가축에 비해 단가가 높고, 생산성 측면에서 농가의 소득과 밀접히 연관되어 있으며 축우의 42%는 밤에 분만이 이루어지고 있어 정확한 분만 예측은 더 중요하다고 할 수 있다. 그리하여 본 논문에서는 경구 투여용 센서를 통해 반추위 내의 심부 체온을 외부 환경의 간섭 없이 안정적으로 실시간 측정하고 이를 딥러닝에 적용함으로써 분만 시점을 예측하는 방법을 제안 하였고, 실제 축우를 대상으로 실험을 수행한 결과 실제 분만 시간 대비 평균 3시간 40분의 오차만 보여 기존 분만 예측 방법보다 정확하게 분만일을 예측하는 것을 확인하였다. 제안하는 방법을 통해 축우의 분만을 정확하게 예측하여 난산의 위험 없이 성공적으로 분만 하도록 도움을 줌으로써 농가의 경제적 피해를 절감할 수 있을 것으로 기대한다.

• 대한수의학회지
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• 제24권1호
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• pp.105-109
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• 1984

Five Korean native goats were employed to test the effectiveness of the two modified techniques (Method I and II) of Pawlow's pouch method, Both methods turned out to be successful and simpler than the original pawlow's pouch method. In both methods, the miniature rumens were formed on the junction of two veins; versa ruminalis sinistra and vena ruminalis dextra. Both method I and II have the advantage of shortening the operation time, but caution is necessary to prevent the fistula formation between the rumen and miniature rumen in Method I.