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A Comparison Study of Metaphase Analysis of Chromosomal Aberration and Flow Cytometric Assessment of Radiation-induced Apoptosis in Human Peripheral Lymphocytes (인체 말초혈액 림프구에서 방사선유도 염색체 손상 및 세포고사에 대한 중기염색체 분석 및 유세포계측 연구)

  • Bom, Hee-Seung;Lee, Seung-Yeon;Lee, Sang-Ku;Min, Jung-Jun;Jeong, Hwan-Jeong;Song, Ho-Cheon;Kim, Ji-Yeul;Shin, Jong-Hee;Suh, Sun-Pal;Rhang, Dong-Wook
    • The Korean Journal of Nuclear Medicine
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    • v.33 no.1
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    • pp.94-99
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    • 1999
  • Purpose: Radiation-induced chromosomal damage and apoptosis were compared in human lymphocytes. Materials and Methods: Peripheral lymphocytes from 10 normal volunteers (6 males, 4 females, age range $23{\sim}41$ years) were irradiated by gamma rays from a cell irradiator. Doses of irradiation were 0 (control), 0.18, 2, 5, 10, 20 and 25 Gy. Irradiated lymphocytes were examined by metaphase analysis for chromosomal aberrations and by flow cytometry for apoptosis. Results of both studies were compared according to dose. Results: Number of dicentric and ring chromosomes (D+R) was $0.5{\pm}0.53$ at baseline, which was significantly increased after radiation according to the dose. The fraction of cells showing annexin V-fluorescein isothiocyanate uptake was $0.51{\pm}$0.39%, which increased to $3.58{\pm}1.85%$ by 2 Gy irradiation, and then decreased. The fraction of cells showing propidium iodide (PI) uptake was $0.52{\pm}0.12%$, which significantly increased according to dose (upto $15.64{\pm}5.99%$ by 20 Gy irradiation). D+R and PI uptake were well correlated (r=0.84, p<0.001). Conclusion: Radiation-induced chromosomal aberration was correlated to nuclear uptake of PI, a marker of late apoptosis.

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Replacement of Obstructed Extracardiac Conduits with Autologous Tissue Reconstructions (Peel operation); Early and Midterm Results (심외도관 협착 환자에서 자가조직을 이용한 재수술(Peel 수술); 조기 및 중기성적)

  • Sung, Si-Chan;Chang, Yoon-Hee;Lee, Choong-Won;Park, Chin-Su;Lee, Hyoung-Doo;Ban, Ji-Eun;Choo, Ki-Seok
    • Journal of Chest Surgery
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    • v.40 no.3 s.272
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    • pp.193-199
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    • 2007
  • Background: Reoperation is usually required for a right ventricle to pulmonary artery conduit obstruction caused by valve degeneration, conduit peel formation or somatic growth of the patient. An autologous tissue reconstruction (peel operation), where a prosthetic roof is placed over the fibrotic tissue bed of the explanted conduit, has been used to manage conduit obstructions at our institute since May 2002. Herein, the early and midterm results are evaluated. Material and Method: Between May 2002 and July 2006, 9 patients underwent obstructed extracardiac conduit replacement with an autologous tissue reconstruction, at a mean of 5.1 years after a Rastelli operation. The mean age at reoperation was $7.5{\pm}2.4$ years, ranging from 2.9 to 10.1 years. The diagnoses included 6 pulmonary atresia with VSD, 2 truncus arteriosus and 1 transposition of the great arteries. The preoperative mean systolic gradient was $88.3{\pm}22.2mmHg$, ranging from 58 to 125 mmHg. The explanted conduits were all Polystan valved pulmonary conduit (Polystan, Denmark). A bioprosthetic valve was inserted in 8 patients, and a monocusp ventricular outflow patch (MVOP) was used in 1 patient. The anterior wall was constructed with a Gore-Tex patch (n=7), MVOP (n=1) and bovine pericardium (n=1). Pulmonary artery angioplasty was required in 5 patients and anterior aortopexy in 2. The mean cardiopulmonary bypass time . was 154 minutes, ranging from 133 to 181 minutes; an aortic crossclamp was not performed in all patients. The mean follow-up duration was 20 months, ranging from 1 to 51 months. All patients were evaluated for their right ventricular outflow pathway using a 3-D CT scan. Resuit: There was no operative mortality or late death. The mean pressure gradient, assessed by echocardiography through the right ventricular outflow tract, was 20.4 mmHg, ranging from 0 to 29.6 mmMg, at discharge and 26 mmHg, ranging from 13 to 36 mmHg, at the latest follow-up (n=7, follow-up duration >1 year). There were no pseudoaneurysms, strictures or thrombotic occlusions. Conclusion: A peel operation was concluded to be a safe and effective re-operative option for an obstructed extracardiac conduit following a Rastelli operation.

A Study on the Sanitary Status at Various Types of Restaurants in Changwon City (창원시 식품접객업소의 위생실태에 관한 조사연구)

  • 이경혜;류은순;이경연
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.30 no.4
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    • pp.747-759
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    • 2001
  • The sanitary status of 264 restaurants was investigated to develop a program of sanitary education at restaurants for improving sanitary levels of restaurant and consumers’ food safety. This investigation was performed through direct interviews on general items and sanitations for employees, facilities, equipments and food treatment. The restaurants are grouped into four different types according to the food served: Korean style food, Japanese style raw fish, roasted ribs, and western style food. It is found that sanitary education for employees are conducted at 66.5% of the total restaurants. The highest percentages are obtained by Korean style food restaurants (83.1%) and the lowest by western style food restaurants (55.6%).Washing facilities for employees are equipped at only 66.8% of the total restaurants. In the personal sanitation, 96.6% of the employees wash their hand after touching a dirty stuff, 77.5% after touching money and 57.1% after using telephone. It is also revealed that during food preparation shoes, overgarments, and caps are worn by 58.5%, 55.5%, and 20.6% of the employees, respectively. 73.5% of the restaurants are equipped with dish storages facilities while only 59.2% of restaurants have sterilizers for dishes. Also, chopping boards are sterilized more than once a week by 74.8% of them and knives everyday by 71.6%, 15.4% of restaurants sterilize their knives only once a week. 56.8% of restaurants check temperatures of the refrigerators and 26.2% of restaurants do not even sterilize the refrigerators. 31.8% of restaurants sterilize the kitchens with sodium hopochlorite after cooking. 93.3% of the restaurants store the raw food and the cooked foods separately. 49.8% of the restaurants refroze thawed food and 19.4% of the restaurants reuse leftovers. The frozen foods are thawed at room temperature by 49.4% of the total restaurants and 66.7% of the roasted rib restaurants.

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Distributional Characteristics and Management Plan for the Floristic and Naturalized Plants of Yeongcheon River in Jinju City (진주시 영천강의 식물상 및 외래식물 분포와 관리방안)

  • Lee, Jae Sook;Park, Sam-Bong;Park, Jeong-Geun;An, Jong Bin;Song, Jin-Heon;Hwang, Jun;Kim, Bong-Gyu;Choo, Gab-Chul
    • Journal of Korean Society of Forest Science
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    • v.108 no.4
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    • pp.493-512
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    • 2019
  • The purpose of this study was to provide the basic data necessary for establishing a vegetation management plan for the Yeongcheon River area by presenting a better understanding of the distribution and characteristics of naturalized plants through an investigation of the flora present in the Yeongcheon riverside, in Jinju, South Korea. Vascular plants were investigated for a total of 470 taxa, including 90 families, 282 genera, 425 species, one sub-species, 38 varieties, and six forms. Together, these accounted for 9.62% of the vascular plants (4,881 species) in Korea. Gramineae was the most abundant at 77 taxa (16.38%), followed by Cyperaceae at 56 taxa (11.91%), Leguminosae at 33 taxa (7.02%), Cyperaceae at 25 taxa (5.32%), and Rosaceae at 22 taxa (4.86%). Thirty species of indicator plants were surveyed and, among them, annual plants and hemicryptophytes accounted for a significantpercentage. Among ground plants, trees, shrubs, and sub-shrubs were surveyed to include 23 (4.89%), 17 (3.61%), and 14 taxa (2.97%), respectively. Furthermore, 36 aquatic plant taxa were found. Six rare plant taxa were surveyed including Penthorum chinense, Melothria japonica, Aristolochia contorta, Acorus calamus, Millettia japonica, and Magnolia kobus. Floristic special plants comprised 35 taxa,including 26 families, 34 genera, 34 species, and one variety. Plants endemic to Korea included six species. Naturalized plant species comprised 71 taxa, including 18 families, 53 genera, 68 species, and three varieties. The naturalization rate and urbanization index were 15.1% and 22.1%, respectively. Ecological disturbance species in Korea comprised nine taxa, including four families, eight genera, eight species, and one variety. These groups accounted for 64% of the ecological disturbance species in Korea. Ecological disturbance species in Korea tended to increase toward the downstream of Yeongcheon River. In particular, Ambrosia artemisiifolia and Sicyos angulatus, which cause human allergies and disrupt the habitats of plants and animals, must be removed artificially.

Research on Classifying the 'Sijochang', or Korean Ode Narrative Song (시조창 분류고)

  • Shin Woong-Soon
    • Sijohaknonchong
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    • v.24
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    • pp.223-258
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    • 2006
  • This Research is about the classification of 'Sijochang', or the Korean ode narrative song, in terms of music. Contrary to the literature classification by the number of letters, sijochang varies with the melody. Literally, the classification is generally made as Dansijo(or short ode) Jungsijo(or medium ode) and Jangsijo(or lengthy ode) but the sijochang is normally divided into 'Pyongsijo' (or plain ode), 'Jirumsijo and Saseolsijo'. As while the same Sijochang is called under the different names, the different type of sijochang is also called as the same name, it needs the discussion about its name. Some Korean classical musicians have attempted to define it but they are trying to do it without the specific reasoning about its concept. As a result, the systematic research is required. This study designs to streamline the currently confusing and complex names and set up the sijo's classification system. After reviewing the ancient music note, current sijo score and the traditional theory, I largely classified it into 3 types: Pyongsijo, Jirumsijo and Saseolsijo. And then, 1 analyzed on to which type the sijochang which is presently called belongs, based on several principles. The 67 names of the sijo which I have investigated about are classified with them sharpy reduced into 16. Among the current sijo names. there are some which are of same type yet of different phonetics and there are others which are of different phonetics yet of same type. To avoid such complex and troublesome names, I have orchestrated them as follows, taking the literary and music concept into account. 1) Pyongsijo type : Pyongsijo, Joongherisijo, Wujosijo and Payeonkok 2) Jirumsijo type: Jirumsiro, Namchangjirumsijo(it refers to Jirumsijo sung by male ), Yeochangjirumsijo (it refers to Jirumsijo sung by female), Banjirumsijo(it refers to half the Jiumsiro), Onjirumsijo (it refers to the whole Jirumsijo), Wujojr\irumsijo, Saseoljirumsijo and Whimorisijo) 3) Saseolsijo type : Saseolsijo, Bansaseolsijo(it refers to half the Saseolsijo, Gaksijo or Pyongsiro There are still lots of things to musically streamline, in the fields of disposition of Sijo letters, its form, musical scale and influences on other genre. etc. and as such. the accumulation of theory on them is urgently required. Those musical elements need an in-depth review and study by the experts and the Korean traditional musicians. Later research is expected to play a role of exploring it.

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A Statistical Study of the Foreign Bodies in the Food and Air Passages for past 4 years and 5 months (과거 4년 5개월간 본교실에서 취급한 식도 및 기도이물의 통계적 고찰)

  • 전지일;김성일;조현상;김정원;최태규;김진구;최병익;임현준;김영학
    • Proceedings of the KOR-BRONCHOESO Conference
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    • 1976.06a
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    • pp.87.4-88
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    • 1976
  • The statistcal study was done on 81 cases of the foreign bodies in the food passages (73 cases) and air passages (8 cases), who had visited the Dept. of Otolaryngology of Kyung Hee Medical Center and treated endoscopically, from Nov. 1971 to Mar 1976. The results were as follows, 1) The incidence of esophageal foreign bodies (73 cases) in order of frequency was coin (61 cases; 83.6%), bone (6 cases; 8.2%), food particles (3 cases; 4.1%), discs (2 cases), and other metals (l case). The incidence of foreign bodies in the air passages (8 cases) was food particles (3 cases; 37.5%), pointed metal (2 cases; 25%), plastic pencil cap (l case), plastic ring (1 case), and wood piece (1 case) in order. 2) In sex distribution, 51 cases (62.9%) were males and 30 cases (37.1%) were females, and the ratio between males and females was about 1.7 : 1.3. In the age incidence, 69 cases (82.5%) of all foreign bodies were under 5 years of age, and especially, coin cases were 59 cases (96.7%) and the highest was 8 years of age, lowest was 11 months of age. 7 cases (87.5%) of all airway foreign bodies were under 10 years of age. 4) The ratio between the food passages(73 cases) and the air passages (8 cases) was about 9 : 1. In the location of the foreign bodies of the food passages, 66 cases (90.4%) were in the first esophageal narrowing, 5 cases (6.9%) in the second narrowing, and 2 cases (2.7%) in the third narrowing. In the air passages, the frequent sites were trachea (5 cases; 62.5%), larynx (2 cases), and bronchus (1 case) in order. 5) In duration of lodgement, 63 cases (86. 3%) of esophageal foreign bodies were removed within 24 hours, and one case was removed within 16 days. The number of cases lodged within 24 hours were 4 cases (50%). in the air passages and one case was removed within 18 days. 6) In treatment, 37 cases (50.7%) of esophageal foreign bodies were extracted by modified upper esophagoscopy, 33 cases (42.5%) by esophagoscopy under the local anesthesia, 3 cases (4.1%) by esophagoscopy under the general anesthesia. 8 cases of the coin cases were passed into the stomach during the endoscopic examination. Laryngeal foreign bodies (2 cases; 25%) were extracted by laryngoscopy under the local anesthesia. 2 cases (40%) of tracheal foreign bodies by Bronchoscopy with tracheotomy under the local anesthesia, 3 cases (60%) by Bronchoscopy under the general anesthesia, and bronchial foreign body (1 case) by open thoracotomy under the general anesthesia, at the Dept. of Chest surgery.

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Study on Vegetation Analysis for Indicators Development of Agro-ecosystem Habitat Quality (농업생태계의 서식지 질 지표 개발을 위한 식생분석)

  • Park, Kwang-Lai;Kang, Bang-Hun;Choi, Jae-Woong;Kim, Chang-Hwan
    • Korean Journal of Soil Science and Fertilizer
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    • v.43 no.6
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    • pp.1040-1046
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    • 2010
  • This research is composed of a series of survey of existing plants species by classifying biotope type of agro-ecosystem of Guksoo village area of Yangpyeong County, to collect and analyze basic data of vegetation analysis for indicators development of agro-ecosystem habitat quality. From the observation area, we found total 141 kinds of tracheophytes (53 Family 114 Genus 124 Species 16 Variety 1 Breed) and they are 3.36% of total Korean tracheophytes (4,191 kinds). Among those 141 tracheophytes, there are 23 kinds of naturalized plants (11 Family 20 Genus 20 Species 2 Variety) and they are 8.61% of total Korean naturalized plants (267 kinds). Among those 141 tracheophytes, they include 0.71% of pteridophyte, 0.71% of gymnosperm, 98.58% of angiosperm. So, most of them are angiosperm. When we classify them according to plant life form characteristics, dormant/diapause type plants include 45 species (31.91%) of annual plant (Th), 19 species (13.48%) of Th (w), 17species (12.06%) of hemicryptophyte (H). Regarding propagation type, as for the Radicoid form, there are 99 species (70.21%) of crumb structure plant, 13 species (9.22%) of $R_4$, 12 species (8.51%) of $R_{2.3}$ are the crumb structure does not make any connection on the ground or under ground. As for the Disseminule form of propagation type, there are 62 species (43.97%) of Gravity dispersal type $D_4$), 23 species (16.31%) of Wind dispersal type ($D_1$), 21 species (14.89%) of $D_{1.4}$. According to this survey of plant distribution rate by plant life form characteristics, we may acquire many knowledge about species composition of sociability, cluster's reaction against environmental elements, space usage and possible species competition in community. It may be very useful basic data for habitat preservation to keep and promote biological diversity.

Development of 3D Printed Snack-dish for the Elderly with Dementia (3D 프린팅 기술을 활용한 치매노인 전용 영양(수분)보충 식품섭취용기 개발)

  • Lee, Ji-Yeon;Kim, Cheol-Ho;Kim, Kug-Weon;Lee, Kyong-Ae;Koh, Kwangoh;Kim, Hee-Seon
    • Korean Journal of Community Nutrition
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    • v.26 no.5
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    • pp.327-336
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    • 2021
  • Objectives: This study was conducted to create a 3D printable snack dish model for the elderly with low food or fluid intake along with barriers towards eating. Methods: The decision was made by the hybrid-brainstorming method for creating the 3D model. Experts were assigned based on their professional areas such as clinical nutrition, food hygiene and chemical safety for the creation process. After serial feedback processes, the grape shape was suggested as the final model. After various concept sketching and making clay models, 3D-printing technology was applied to produce a prototype. Results: 3D design modeling process was conducted by SolidWorks program. After considering Dietary reference intakes for Koreans (KDRIs) and other survey data, appropriate supplementary water serving volume was decided as 285 mL which meets 30% of Adequate intake. To consider printing output conditions, this model has six grapes in one bunch with a safety lid. The FDM printer and PLA filaments were used for food hygiene and safety. To stimulate cognitive functions and interests of eating, numbers one to six was engraved on the lid of the final 3D model. Conclusions: The newly-developed 3D model was designed to increase intakes of nutrients and water in the elderly with dementia during snack time. Since dementia patients often forget to eat, engraving numbers on the grapes was conducted to stimulate cognitive function related to the swallowing and chewing process. We suggest that investigations on the types of foods or fluids are needed in the developed 3D model snack dish for future studies.

The Drawing of Linked-arc Designs in Bronze Mirrors and The Technological Genealogy of the Three-Hans Style Bronze Mirror (연호문(連弧文)의 제도와 삼한경(三韓鏡)의 기술 계보)

  • Yi, Yangsu
    • Korean Journal of Heritage: History & Science
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    • v.54 no.1
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    • pp.164-183
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    • 2021
  • This article examines how the production technology of the Chinese bronze mirror affected the Korean Peninsula and Japan. To that end, we looked at how the drawing of linked-arc designs in bronze mirrors was done. The drawing technique is classified into three methods: Type 1 and type 2 were confirmed to have been used in China, but type 3 was used not in China, but on the Korean Peninsula and in Japan. Type 1 and type 2 are basically the same, with an identical restored diameter of the linked-arc design, and type 2 is found elsewhere in the bronze mirror, and type 1 are not. Type 3 features different diameters, and the linked-arc design does not form a complete circle. This shows that the manufacturer of the bronze mirror did not use several bumpy compasses, but rather a single one with a fixed center and protrusion, rather than one that can be easily adjusted, as it is today. That is why, of course, a pair of compasses must have been used several times. In this regard, those who produced bronze mirrors on the Korean Peninsula in the early days understood not only the Chinese techniques of the linked-arc design, but also the meaning contained within them. On the other hand, the makers of bronze mirrors in Japan did not understand the patterns and simply imitated them. From the late Yayoi period, Japan understood and produced works with the principle of drawing the linked-arc design. As a representative example, bronze mirrors excavated from the tomb of Hirabaru can be referenced. Perhaps it can be assumed that, behind these works, there was a direct transfer of technology, such as the migration of the makers of bronze mirror makers like the Do family (陶氏), whose names were found written on bronze mirrors excavated from the Hirabaru tomb.

Comparison of capsule type resin modified glass ionomer porosity according to mixing methods (혼합방법에 따른 캡슐형 광중합글라스아이노머의 공극률 비교)

  • Kim, Jung-min;Kim, Jin-Woo;Cho, Kyung-Mo;Lee, Yoon;Kim, Eung-Hyun;Park, Se-Hee
    • Journal of Dental Rehabilitation and Applied Science
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    • v.37 no.4
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    • pp.217-224
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    • 2021
  • Purpose: The purpose of this study was to evaluate the porosity of resin modified glass ionomer (RMGI) by different mixing methods. Materials and Methods: Five specimens were prepared for each groups according to capsules and mixing methods. Two RMGI capsule and two mixing machines were used for this study. One resin-modified glass ionomer cement is Fuji II LC (F2LC) and the other is Photac Fil Quick Aplicap (PFQ). For Mixing of RMGI capsule, Rotomix using rotating motion and CM-II using shaking motion were used. After measuring height, radius and mass of specimens, Density was calculated. And porosity was measured using micro-computed tomography (micro-CT). All data were statistically analyzed using T-test, two-way ANOVA to compare between groups at 95% significance level to evaluate the affect of capsule and mixing method on the porosity. Results: The porosity was observed in all specimens generally. And there is significant differece between porosities according to RMGI capsule and Mixing method. The porosity of PFQ was lower than that of F2LC and the porosity of Rotomix was lower than that of CM-II. Conclusion: There was a difference of porosity according to kind of capsules and mixing methods. When using same capsule, less porosity was observed on PFQ than F2LC. When using same mixing mehod, less porosity was observed on Rotomix than CM-II. Using mixing machine of same coporation as that of RMGI capsule did not lead to lower porosity. Therefore, Selecting optimal mixing machine is important.