• Journal of Plant Biotechnology
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• v.36 no.3
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• pp.281-288
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• 2009

Seed germination is the important stage to express many genes for regulation of energy metabolism, starch degradation and cell division from seed dormancy state. For the functional analysis of seed germination mechanisms, we were analyzed the rice cDNA clones (Oryzasativa cultivar Ilpum) obtained from seed imbibition during 48 hours. Total number of 18,101 Expressed Sequence Tags (ESTs) were clustered using SeqMan program. Among them, 8,836 clones were identified as unique clones. We identified the chitinase gene specifically expressed in seed germination and amylase gene involved to starch degradation from the full length cDNA analysis, and several genes were registered to NCBI GeneBank. To analyzed the commonly expressed genes between inmature seed and germinated seed, 25,66 inmature ESTs and 18,101 germinated ESTs were clustered using SeqMan program and identified 2,514 clones as commonly expressed unigene. Among them, alpha-glubulin and alcohol dehydrogenase I were supposed to LEA genes only expressed in the immature and germinated seed stages. For the clustering of orthologous group genes, we further analyzed the 8,836 EST clones from germinating seeds using NCBI clusters of orthologous groups database. Among the clones, 5,076 clones were categorized into information storage and processing, cellular processes and signaling, metabolism and poorly characterized genes, proportioning 783 (14.29%), 1,484 (27%), 1,363 (24.8%) and 1,869 (34%) clones to the previous four categories, respectively.

• Journal of Plant Biotechnology
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• v.40 no.1
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• pp.55-58
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• 2013

Immature embryos of Glycine max L. was cultured on Murashige and Skoog's (MS) medium supplemented with 1 mg/L 2,4-dichlorophenoxy acetic acid (2,4-D). After 6 to 8 weeks of culture, immature embryos produced somatic embryos. Of somatic embryos, two cotyledonary embryo (14%), one cotyledonary embryo (37%), fused cotyledonary embryo (43%), and stunted globular embryos (6%) were observed. The procambial strand of cotyledons originated from circular procambial tissues of lower hypocotyl. The circular procambial tissues were independently divided into one or two procambial strand at the edge of cotyledonary-node, and then connected to each cotyledon to form somatic embryos with one or two cotyledons. When cotyledon was a fused type, the circular procambial strand in lower hypocotyl was continuously connected to the cotyledon. Also, somatic embryos with two cotyledons developed a functional shoot apex with the tunica-corpus structure. In contrast, somatic embryos with one or fused cotyledon formed an abnormal shoot apex without the tunica-corpus structure or with non-dome shape in the inter-cotyledonary area. These results indicated that the variation of cotyledon in somatic embryos is closely related to procambial differentiation and shoot apical meristem development.

• Korean Journal of Veterinary Service
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• v.42 no.4
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• pp.193-200
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• 2019

Blood test is a useful tool in establishing medical treatment for livestock. It provides information such as disease diagnosis, treatment effects, prognostic judgment, and health status. This study compared the value of erythrocytes and leukocytes among conventional, transgenic miniature, and transgenic conventional pigs aged six months to 24 months. Further, it analyzed the aspects of hematological value changes according to the pigs' ages. As a result, the number of red blood cells (RBC), which include hemoglobin, and hematocrit, and the number of white blood cells (WBC), which include neutrophils, and lymphocyte, were high among transgenic miniature pigs, compared with the conventional and transgenic conventional pigs. Conventional pigs showed similar values of RBC and WBC regardless of transgenesis. In comparing their age, the RBC decreased as the age increased compared with the pigs among all the three groups aged of 6~12 months. On the other hand, WBC and neutrophils showed no significant difference regardless of different ages among all the three groups. However, various counts in RBC and WBC were mostly found to be higher in each age in transgenic miniature pigs than in conventional and transgenic conventional pigs. The results of this study show that the values of RBC and WBC were generally higher in transgenic miniature pigs than in conventional and transgenic conventional pigs. Based on this research, hematological values can be widely used in diagnosing diseases or checking the health status of transgenic pigs that are used as disease models, organ transplant source and alike.

• Korean Journal of Applied Biomechanics
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• v.25 no.3
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• pp.335-342
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• 2015

Objective : The purposes of this study was to analyze the effects of the stirrup length fitted to the rider's lower limb length and it's impact on less skilled riders during trot in equestrian events. Methods : Participants selected as subjects consisted of less skilled riders(n=5, mean age: $40.02{\pm}10.75yrs$, mean heights: $169.77{\pm}2.08cm$, mean body weights: $67.65{\pm}7.76kg$, lower limb lengths: $97.26{\pm}2.35cm$, mean horse heights: $164.00{\pm}5.74cm$ with 2 type of stirrups lengths(lower limb ratio 74.04%, and 79.18%) during trot. The variables analyzed consisted of the displacement for Y axis and Z axis(head, and center of mass[COM]) with asymmetric index, trunk front-rear angle(consistency index), lower limb joint(Right hip, knee, and ankle), and average vertical forces of horse rider during 1 stride in trot. The 4 camcorder(HDR-HC7/HDV 1080i, Spony Corp, Japan) was used to capture horse riding motion at a rate of 60 frames/sec. Raw data was collected from Kwon3D XP motion analysis package ver 4.0 program(Visol, Korea) during trot. Results : The movements and asymmetric index didn't show significant difference at head and COM, Also, 74.04% stirrups lengths in trunk tilting angle showed significant difference with higher consistency than that of 79.18% stirrups lengths. Hip and knee joint angle showed significant difference with more extended posture than that of 74.04% stirrups lengths during trot. Ankle angle of 79.18% stirrups length showed more plantarflexion than that of 74.04% stirrups lengths. Average vertical force of rider showed significant difference with higher force at 79.18% stirrups lengths than that of 74.04% stirrups lengths during stance phase. Conclusion : When considering the above, 74.04% stirrups length could be effective in impulse reduction with consistent posture in rather less skilled horse riders.

• Korean Journal of Soil Science and Fertilizer
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• v.39 no.2
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• pp.86-94
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• 2006

The primary concern on fertilizer recommendations on paddy soils in Korea is not high rice yield but high rice quality, sustainable rice yield, and less environmental loads these days. Based on soil survey data, the paddy soils in Korea were classified into five large management types ; normal, sandy, immatured, poorly drained, saline soil. In order to establish the optimum level of nitrogen fertilizer to increase the rate of head rice yield, field experiments were conducted at 24 farmhouses throughout the country with nitrogen fertilizer treatment levels of 0, 50, 70, 90, 110, 140, $170kg\;ha^{-1}$ from 2003 to 2004. As the result of the experiment, the optimum rates of nitrogen fertilizer for improving rice quality were $90kg\;ha^{-1}$ in normal, sandy, and poorly drained soils, $100kg\;ha^{-1}$ in immatured soils, and $112kg\;ha^{-1}$ in saline soils where the content of NaCl in soil was below 0.1%. The optimum rates of nitrogen fertilizer were determined in view of head rice percent, protein content, and palatability value of rice.

• Korean Journal of Soil Science and Fertilizer
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• v.25 no.2
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• pp.160-167
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• 1992

The growth of weeds and some other plants has been considered to be inhibited by sicklepod(Cassia tora L.) sharing the habitat. The study was conducted, for the first time, to propose that this phenomenon is not only due to competition for physical and nutritional conditions but also due to allelopathy. In addition, autotoxicity of sicklepod was examined. The results are summarized as follows. 1. Rice sheath length reduced progressively from 18 % to 36 % with increasing the concentration of treating aqueous extracts of sickle pod seeds, but rice germination was not affected. In contrast, radish hypocotyl length was not reduced by the aqueous extract treatment but radish germination was significantly reduced by 66 % at 1 : 10 and 1 : 5 treatment. 2. Total chlorophyll contents in rice seedling decreased from 50 % to 65 % by treatment of seed aqueous extracts diluted from 1 : 50 to 1 : 5 ratio. 3. Aqueous extracts of sicklepod leaves significantly reduced hypocotyl length and fresh weight in radish and germination in rice, but mung bean was slightly affected by aqueous extracts only in fresh weight. 4. Volatiles from fresh, immature seeds with husk reduced the radish germination and seedling growth and radish root growth appeared to be more sensitive to the exposure to volatiles from fresh immature seeds than both germination and hypocotyl growth. 5. Volatiles from sickepod leaves inhibited germination and growth of radish, rice and mung bean, and seedling growth was more sensitive to volatiles from leaves than germination. 6. Volatiles from sicklepod leaves reduced germination and radicle length of sicklepod itself. 7. Collectively, it is concluded that there are water-soluble and volatile substances responsible for allelopathy in sicklepod.

• Journal of Food Hygiene and Safety
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• v.19 no.4
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• pp.203-208
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• 2004

The shells of Anadarac tegillarca granosa, Crassostrea gigas, Crassostrea nippona, and Patinopecten yessoensis were used for preparation of calcium carbonate with high purity. Calcium content in ash of shell was the highest $64.9\%$ in Anadarac tegillarca granosa ashed for 5 hr at $900^{\circ}C$, and followed as Patinopecten yessoensis $62.5\%$, Crassostrea gigas $62.4\%$, and Crassostrea nippona $61.5\%$. Whiteness of ash was the highest 81.6-85.8 in Patinopecten yessoensis shell. Calcium contents in calcium carbonates made with shells of Anadarac tegillarca granosa by using ammonium chloride process (ACP) and ammonium nitrate process (ANP) were higher $40.03-40.04\%$ than $39.92\%$ in Anadarac tegillarca granosa ash. Calcium content was the highest $40.04\%$ in pH adjusted calcium carbonate prepared by using ANP. Whiteness of calcium carbonate prepared by using ACP and ANP was the level of 101.0-101.5. Therefore, we estimated that the calcium carbonate made by using ACP and ANP could be used potentiality as a food additive for calcium supplement.

• Tuberculosis and Respiratory Diseases
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• v.66 no.2
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• pp.127-131
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• 2009

Most mediastinal teratomas are histologically well-differentiated tumors and benign. The majority of patients with a mediastinal teratoma are asymptomatic and their tumors are usually discovered incidentally on chest radiography. On rare occasions this tumor will rupture spontaneously into the adjacent organs. A 72-year-old female patient was admitted for dyspnea and she had a multiloculated pleural effusion in the left lung field. Although repeated pleural biopsy and pleural fluid cytology did not prove the presence of malignancy, we assumed that this was a malignant effusion because it revealed consistently high levels of carcinoembryonic antigen and carbohydrate antigen 19-9, and the chest CT scan did not show typical fat or bone density in the mass. Secondary infection and an uncontrolled septic condition due to pleural empyema finally compelled the patient to undergo a surgical operation. Mature teratoma was the final diagnosis and she has done well without recurrence for 2 months.

• Korean Journal of Childcare and Education
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• v.7 no.3
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• pp.43-65
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• 2011

The purpose of this study was to investigate the current status of English education for toddlers at home and institutes, and influential variables upon mothers' demand on English education for their toddlers. Four hundred forty-one mothers who have toddlers under 36 months-old were surveyed using a questionnaire. Data were analyzed using frequency and logistic regression. Results of this study were as follows: It was found that 30.9% of mothers were providing English education to their toddlers. They started English education when their toddlers were between 24 months and 36 months because of parents' decision or English education in child care centers. English education was mostly given to their toddlers once or twice a week, less than 30 minutes a time with Korean teachers who majored in English. English lessons were focused on simple dialogue using multi media, worksheets, and workbooks. In other sense, 66.0% of mothers showed a positive perspective toward English education for toddlers. The higher salary, years of education, and degree of agreement on English education based on physiological, linguistic, and social perspectives were, the stronger the demand on English education was. Results of this study suggested that appropriate guidance for English education for toddlers needs to be provided.

• The Korean Journal of Emergency Medical Services
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• v.16 no.1
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• pp.65-80
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• 2012

Purpose: The objective of this study was to assess the job satisfaction and turnover intention of the emergency medical technicians (EMT) in the private ambulance service, to examine their job satisfaction and turnover intention, and to provide the solution for the management of the private ambulance service. Methods: The survey was conducted with 121 EMTs (73 paramedics, 36 basic EMTs and 12 nurses) in the private ambulance service in Korea from September 15 to October 14, 2011. In the reliability of the questionnaire, Cronbach's ${\alpha}$ was 0.790 for job satisfaction and 0.796 for turnover intention. Using SPSS 18.0, we obtained Cronbach's ${\alpha}$, frequencies, percentages, means, and standard deviations, and performed independent t-tests, ANOVA, and Pearson's correlation analysis. Results: 1) The mean score with regard to job satisfaction was 2.94 and that of their turnover intention was 4.23. In the area of job satisfaction, the mean score of the job demand area was 4.23; 3.97 for the job itself; 3.07 for the area of interaction; 2.98 for the area of autonomy: 2.67 for the organizational demand area; 2.67 for working conditions area; and 1.73 for the wage area. 2) Statistically significant difference was observed in job satisfaction according to age (F=3.819, p=.006), wages (t=-4.640, p=.000), terms of incumbency (F=3.868, p=.011), and in turnover intention it was according to sex (t=-1.995 p=.048), age (F=9.611, p=.000), education levels (F=6.974, p=.002), marital status (t=4.393, p=.000), wages (t=5.515, p=.000), license types (F=8.481, p=.001), and terms of incumbency ( F=14.115, p=.000). 3) The job satisfaction and the turnover intention had a negative correlation to each other (r=-.56, p<.000) in general, and in the sub-7 areas of job satisfaction, the correlation with turnover intention was high in order of the wage area (r=-.61, p=.000), working conditions area (r=-.52, p=.000), the area of autonomy (r=-.49, p=.000), the area of interaction (r=-.45, p=.000), the organizational demand area (r=-.40, p=.000), the job itself (r=-.24, p=.007) and the job demand area (r=-.24, p=.009). Conclusion: The government must take the charge of lowering the turnover intention among paramedics in the private ambulance service by providing the advantage in wages and fringe benefits. Ultimately, this would bring an improvement in the quality of medical emergency services to hospitals especially in the area of patient transfer and transportation.