• 대한기계학회:학술대회논문집
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• 대한기계학회 2008년도 추계학술대회A
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• pp.1683-1684
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• 2008

For direct detection of microbes in air, samples have to be collected but environmental particles such as dust are also trapped in such samples. Therefore the isolation of target bacteria from non-biological materials of similar size is of great importance in the identification of such organisms. Dielectrophoresis is an emerging technique that can rapidly separate cells in microfluidics. In this paper we proposed a new method for the separation of airborne microbes using condensation and dielectrophoresis. This system could be used as a continuous flow through separation system for various particles and utilized as a pretreatment technique for microbe detection.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2008년도 추계학술대회A
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• pp.1629-1632
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• 2008

Rapid, real-time detection of pathogenic microorganisms is an emerging and quickly evolving field of research, especially with regard to microorganisms that pose a major threat to public health. Herein, a new method that uses bioimpedance and solid culture medium for the real-time detection of microorganisms is introduced. We fabricated a new impedimetric biosensor by integrating solid media and two plane electrodes attached on two facing sides of an acryl well. During bioelectrical impedance analysis, the solid medium showed the characteristics of a homogenous conductive material. In a real-time impedance measurement, our solid-medium biosensor could monitor bacterial growth in situ with a detection time of ${\sim}4$ hrs. Our data indicate that the solid-medium biosensor is useful for detecting airborne microorganisms, thereby providing a new analytical tool for impedance microbiology.