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Antioxidant Activity of Different Parts of Lespedeza bicolor and Isolation of Antioxidant Compound (싸리나무(Lespedeza bicolor) 부위별 추출물의 항산화 활성 및 항산화물질 분리)

  • Lee, Jae-Hak;Jhoo, Jin-Woo
    • Korean Journal of Food Science and Technology
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    • v.44 no.6
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    • pp.763-771
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    • 2012
  • In this study, total antioxidant properties of extracts from different parts of Lespedeza bicolor were determined using techniques of measuring 1,1-diphenyl-2-picryl hydrazyl/2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)-radical scavenging activity and total phenolic contents. The total antioxidant activities of leaf, stem and root extracts from various solvents (water, 50, 70, 100% ethanol, and hot-water) indicated that 50 and 70% ethanol extracts have high radical scavenging activities and phenolic contents. A systematic approach was used to determine the total antioxidant activity of different solvent fractions of the Lespedeza bicolor extracts, partitioning with chloroform, ethyl acetate, n-butanol, and water, and the ethyl acetate fraction was found to have the strongest antioxidant activity. Antioxidant assay-guided isolation was carried out to isolate potential antioxidant compounds. The ethyl acetate fraction of the leaf extract was subjected to silica gel, LH-20 and RP-18 column chromatography successively, and afforded compound 1, which was identified as eriodictyol by NMR and MS analysis, after which its antioxidant activity was determined.

구기자(Lycium chinence Miller) 첨가 요구르트의 항산화 활성과 ACE 저해 및 ${\alpha}-glucosidase$ 저해 효과

  • Bae, Hyeong-Cheol;Jo, Im-Sik;Nam, Myeong-Su
    • Proceedings of the Korean Society for Food Science of Animal Resources Conference
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    • pp.326-330
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    • 2005
  • 구기자, 구기엽, 지골피 추출물을 이용한 요구르트를 제조하여 항산화효과, ACE (angiotensin converting enzyme) 저해 효과 및 ${\alpha}-glucosidase$ 저해 활성을 시험하였다. 구기자 첨가 발효유의 Antioxidants activity는 물 추출물보다 methanol 추출물에서 더 많은 항산화 활성능을 보였으며 특히 구기엽 요구르트에서 83.85%의 높은 활성능을 보였다. 구기자와 지골피 요구르트에서는 각각 47%와 54%내외의 활성능을 나타냈다. 구기자 첨가 발효유의 ACE(angiotensin converting enzyme) 저해 효과는 원재료에서는 물 추출물이 angiotensin converting enzyme 저해능이 높았고, 구기자 요구르트의 경우 물 추출물과 methanol 추출물 모두가 angiotensin converting enzyme 저해능이 높은 것으로 나타났다. 구기자 첨가 발효유의 ${\alpha}-glucosidase$ 저해 활성은 구기자, 구기엽 및 지골피 4% 첨가 요구르트에서 methanol 추출물의 경우 각각 8.2%, 5.6%, 7.3%의 ${\alpha}-glucosidase$ 저해능을 나타냈는데 이들 모두 원재료 보다는 활성 저해능이 큰 것으로 나타났으나 물 추출물의 경우에는 활성 저해능이 거의 나타나지 않았다.

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Analysis Technique for Chloride Penetration in High Performance Concrete Behavior Considering Time-Dependent Accelerated Chloride Diffusivity (촉진염화물 확산계수의 시간의존성을 고려한 고성능 콘크리트의 염화물 침투 해석기법)

  • Kwon, Seung-Jun;Park, Sun-Gyu
    • Journal of the Korea Concrete Institute
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    • v.25 no.2
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    • pp.145-153
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    • 2013
  • Recently, accelerated chloride diffusion coefficients are used for an evaluation of chloride behavior. Similar as apparent diffusion coefficients, accelerated diffusion coefficients decrease with time. In this study, decrease in diffusion coefficient with time is simulated with porosity. Utilizing DUCOM-program, porosities from 15 mix proportions are obtained and diffusion coefficients are modelled with regression analysis of porosity for 270 days. Considering non-linear binding capacity which means the relation between free and bound chloride ion, chloride behavior in high performance concrete is evaluated. Through utilizing the previous test results for concrete under chlorides for 180 days, the applicability of the proposed technique is verified. The proposed technique is evaluated to reasonably predict the chloride behavior in concrete with various w/c (water to cement) ratios and mineral admixtures (GGBFS and FA). It is also shown that decrease in chloride diffusion should be considered for chloride prediction in concrete with mineral admixture since it has very clear decrease in diffusivity with time.

Antioxidant Effect and Tyrosinase Inhibition Activity of Seaweeds Ethanol Extracts (해조류 에탄올 추출물의 항산화 및 Tyrosinase 억제 활성)

  • Lee, Na Young
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.42 no.12
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    • pp.1893-1898
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    • 2013
  • Seaweeds, laver, sea mustard, kelp, and fusiformis, were prepared and investigated for its antioxidant and tyrosinase inhibition activities. The extracts yield, color, total phenolic contents, antioxidative activity, and tyrosinase inhibition activity of the extract samples were measured. Hunter Lightness values of laver, sea mustard, kelp, and fusiformis extracts were 82.88, 78.53, 83.04, and 78.11, respectively. The contents of total phenolic compounds of the seaweed extracts powder, laver, sea mustard, kelp, and fusiformis were 43.23, 11.59, 10.09, and 46.59 mg/g of sample, respectively. 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity of the fusiformis extract was shown to be the highest value compared with other seaweed extracts. 2,2'-azino-bis-3-ethylbenzo-thiazoline-6-sulfonic acid (ABTS) radical scavenging activities of laver, sea mustard, kelp, and fusiformis extracts were 258.00, 219.26, 95.77, and $1186.62{\mu}mol$ trolox equivalence per gram, respectively, at the 1,000 ppm level. TBARS value of oil emulsion, samples without extracts was higher than those of the samples prepared with laver and sea mustard extracts. The inhibition rates (%) of the mushroom tyrosinase of laver, sea mustard, kelp, and fusiformis extracts powder were 25.93, 26.32, 24.76 and 20.24% at 1,000 ppm, respectively. The results indicated that laver, sea mustard, kelp and fusiformis extracts possess biological activities such as antioxidant activity and tyrosinase inhibition effect.

The Study for Antioxidative Effects of Acanthopanax sessiliflorus Extract as a New Cosmetics Ingredients Using Electron Paramagnetic Resonance (전자상자성공명법을 이용한 오가피나무추출물의 항산화효과에 대한 연구)

  • Choi, Shin-Wook;Kim, Chang-Su;Choi, Myoung-Soo;Kim, Bo-Hyeon;Kim, Hack-Soo;Choi, Dae-Sung
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.31 no.1
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    • pp.73-78
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    • 2005
  • This work was carried out to investigate the antioxidative effects of Acanthopnax sessiliflorus from Jeongseon County for the purpose of development of a novel antioxidant from natural products. The antioxidant activity was determined by using electron paramagnetic resonance (EPR), not measuring the radical scavenging effect on 1,1-diphenyl-2-picrylhydrazyl (DPPH) which have bun used for antioxidant activity of natural sources. Although DPPH radical scavenging activity assay have been generally used for antioxidant activity, this assay is nut appropriated for determinating which radical is scavenged by extracts from natural products. Using EPR, we determinated whether A. sessiliflorus extracts from Jeongseon County scavenge specific radicals or not. On experiment of scavenging superoxide anion radical, hydroxyl radical, nitrogen dioxide and peroxinitrite. Extracts from A. sessiliflorus showed high antioxidant activities to reactive oxygen and nitrogen species. These result suggest that extracts from A. sessiliflorus act as an antioxidant by scavenging reactive oxygen and nitrogen species and used as new cosmetic ingredients for anti-oxidative stress in skin.

Antioxidant Activity and Inhibitory Effect of Taraxacum officinale Extracts on Nitric Oxide Production (서양민들레 부위별 추출물의 항산화활성 및 nitric oxide 생성저해효과)

  • Min, Kyung-Chun;Jhoo, Jin-Woo
    • Korean Journal of Food Science and Technology
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    • v.45 no.2
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    • pp.206-212
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    • 2013
  • In the current study, antioxidant properties of extracts from different parts of Taraxacum officinale were determined by measuring the radical scavenging activity of 1,1-diphenyl-2-picryl hydrazyl(DPPH) and 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). The antioxidant activity of aerial and root parts of this plant was measured in extracts obtained from various solvents, e.g., water, 50, 70, 100% ethanol, and hot-water. The hot-water extracts had the highest radical scavenging activities. A further study was conducted to determine the total antioxidant activity of hot-water extract fractions obtained from different solvents, e.g., chloroform, ethyl acetate, n-butanol, and water. The ethyl acetate fraction of this extract displayed the strongest DPPH and ABTS radical scavenging activity and the highest total phenolic contents. The inhibitory effect of individual solvent fractions on the production of nitric oxide in LPS-stimulated RAW 264.7 cells was measured. The results indicated that the ethyl acetate fraction of aerial and roots part extracts significantly reduced nitric oxide productions.

Evaluation of Antioxidant and Antimicrobial Activities of Solvent Extracts from Coriolus versicolor (구름버섯(Coriolus versicolor)의 용매 추출물에 대한 항균 및 항산화 활성 조사)

  • Han, So-Ra;Noh, Min-Young;Lee, Joo-Ho;Oh, Tae-Jin
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.44 no.12
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    • pp.1793-1798
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    • 2015
  • Antioxidant activities of various solvent extracts from Coriolus versicolor were investigated for their total polyphenol content, total flavonoid content, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) cation radical scavenging activity. C. versicolor extracts were produced by organic solvents such as ethanol, ethyl acetate, and methanol. Total polyphenol and flavonoid contents in the ethanol and ethyl acetate extracts were higher than those in the methanol extract. DPPH radical scavenging activity of methanol extract showed the highest value of 80.3%, which was similar to that of ascorbic acid (85.5%). All extracts showed good (>90.0%) ABTS cation radical scavenging activity. The antimicrobial activities of these extracts from C. versicolor were evaluated against six strains of bacteria using the disc diffusion method. All extracts showed antimicrobial activities against all tested bacteria except Staphylococcus aureus. These results indicate that various extracts from C. versicolor could be useful as natural antioxidant and antimicrobial agents.

Antioxidative and Antimicrobial Activities of Methanol Extract from Rosmarinus officinalis L. and Their Fractions (로즈마리 추출물 및 분획물의 항균·항산화 활성)

  • Yu, Mi-Hee;Chae, In-Gyeong;Jung, Young-Tae;Jeong, Yeon-Seop;Kim, Hyuk-Il;Lee, In-Seon
    • Journal of Life Science
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    • v.21 no.3
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    • pp.375-384
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    • 2011
  • This study was performed to evaluate the antioxidant and antimicrobial activity of methanol extract from Rosmarinus officinalis L. and its fractions. The ethyl acetate fraction of rosemary had a higher antioxidant activity in both DPPH ($3.22\;{\mu}g/ml$) and ABTS ($5.05\;{\mu}g/ml$) compared to other extracts and fractions. Based on the results of the FRAP assay, the ethyl acetate fraction of rosemary showed a value of $5.9{\pm}0.3\;{\mu}M/{\mu}g$, and buthanol fraction and rosmarinic acid exhibited values of $4.8{\pm}0.2\;{\mu}M/{\mu}g$ and $5.1{\pm}0.1\;{\mu}M/{\mu}M$, respectively. Measurements of the antimicrobial activities of the extracts, fraction against gram positive, negative bacteria revealed that the methanol extract, hexane, ethyl acetate, and chloroform fraction of rosemary caused Staphylococcus aureus and Escherichia coli to form clear zones greater than 12 mm. Furthermore, the methanol extract and chloroform fraction showed high antibacterial activity, with inhibition zone exceeding 13 mm. The methanol extract and chloroform fraction of rosemary had broad antimicrobial spectrums and low MIC values. Therefore, methanol extracts of rosemary could serve as potential antibacterial agents to inhibit pathogen growth in food and hand sanitizers.

Performance Evaluation of Water Loss Management in Urban Areas Using Different Performance Indicators (여러 가지 지표를 이용한 전국도시의 물손실관리 수행능 비교평가)

  • Chung, Shin-Ho;Yu, Myong-Jin;Koo, Ja-Yong;Lee, Hwa-Kyoung
    • Journal of Korean Society of Environmental Engineers
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    • v.28 no.2
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    • pp.117-127
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    • 2006
  • Recently International Water Association(IWA) has proposed new performance indicators for water supply services and their successful applications are being reported continually. According to the various reports, the percentage indicators were suggested not to be used for performance comparison, especially where the targeted areas have great difference in consumption per service connection. But unfortunately in Korea the revenue water ratio, one of the percentage indicators is still being used to set up the goal of water-related administration and to compare the performances between systems. Therefore this study aimed to prove the inapplicability of the percentage indicators with nation-wide data and to suggest better performance indicators for more efficient water loss management. According to the result of the comparison of various performance indicators with conventional one, it is roved that percentage indicators ca not evaluate the performance efficiently where the local situations are significantly different. It is suggested that the better performance indicators such as real losses per service connection or ILI should be used to benchmark the performances of water suppliers on water loss management so thai the problems of water losses could be identified easily and recovered effectively.

Antioxidative Activities of Different Part Extracts of Physalis alkekengi var. francheti (Winter Cherry) (부위별 꽈리(Physalis alkekengi var. francheti) 추출물의 항산화효과)

  • Chung, Hai-Jung
    • Korean Journal of Food Preservation
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    • v.17 no.6
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    • pp.867-873
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    • 2010
  • The total phenolic contents and the antioxidant activities of methanolic extracts of different parts (fruit, calyx, leaf, stem, and root) of Physalis alkekengi var. francheti were investigated using established in vitro systems including DPPH radical-scavenging, nitrite-scavenging, superoxide anion radical-scavenging activity, measurement of reducing power, and assessment of the metal-chelating effect. The highest extraction yield was from fruit (52.55%), whereas the lowest levels were obtained from root (10.49%) and stem (12.88%).The leaf extract had the highest total phenolic (58.47 mg/g) and total flavonoid (4.83 mg/g) contents, plus the greatest antioxidant activity, as shown by the DPPH radical-scavenging assay, and the highest levels of reducing power at concentrations of 1 and 5 mg/mL. In addition, the calyx also showed good antioxidant activity.These findings indicate that methanolic extracts of leaf and calyx may be useful in the food manufacturing and nutraceutical industries.