• Korean Chemical Engineering Research
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• v.56 no.1
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• pp.79-84
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• 2018

In the present study, we performed electroporation to deliver protein into microalgae using previously developed digital electroporation system. Green fluorescence protein was successfully delivered into a live microalgae cell nucleus without cell wall removal. By investigating the effects of applied voltage on the protein delivery efficiency, optimal electroporation electric field condition was found (960 V/cm). We also investigated the delivery of Yo-Pro-1 into cell to examine the size effects of delivered materials and found that there is little size effects on the optimal condition. Finally, the implications of the present results and future work are discussed.

• Proceedings of the Korea Water Resources Association Conference
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• 2018.05a
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• pp.130-130
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• 2018

진해 용원 인근 해역은 부산신항 개발사업으로 추진된 북컨테이너부두 조성사업 및 항만 배후 단지 조성사업으로 전면 해역이 매립되어 원활한 해수흐름이 차단되었고, 인공적인 해양구조물 및 시설물로 인해 해안선 지형 및 단면 변화가 발생하고 이로 인해 수역의 해수순환이 점진적으로 변화하는 지역이다. 인근 어판장으로의 선박의 출입이 차단되면서 발생된 민원으로 인해 송정천 하구 지점의 북쪽 통로를 이용한 선박이동만이 가능한 좁고 긴 수로형태를 가진 폐쇄성 수역으로 변형되었다. 한국에서는 찾아볼 수 없는 이례적인 형태의 용원수로는 폐쇄성 수역이 형성되면서 수로 내로 유입되는 오염물질은 수로 내에 체류되어 수질을 악화시킬 수 있다. 이러한 폐쇄성 수역이 형성되면서 발생하는 다양한 수질영향을 장기간 평가하는 것은 매우 중요하다고 볼 수 있다. 이에 본 연구에서는 용원수로의 시 공간적인 수질변동 양상을 분석하기 위해 2003년부터 2017년까지 선정된 수질측정 지점에서의 주요수질항목에 대한 수질분석을 실시하였으며, 용원수로로 유입되는 토구를 전수조사하고, 비강우시 및 강우시 토구 모니터링을 통해 육상에서 기인하는 오염원을 분석하였다. 또한, 용원수로 내에 유입된 오염물질 거동 분석을 위해 추적자 실험을 통해 용원수로로 유입된 오염원 거동을 예측하여 용원수로의 수질변화 분석에 대한 연구를 실시하였다.

• The Journal of the Korea Contents Association
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• v.13 no.12
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• pp.168-183
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• 2013

Contemporary era has witnessed a diversity of emotion and emotions being expressed through consumption. This paper examines the relationship between social paradigms and emotional design by means of literature study. In sections 2 and 3, I examine the prevalent social paradigms and the concept/development of emotional design. In section 4, I show the correspondence between social paradigms and specific types of emotional design. More specifically it is shown that "pluralism and decentrality" has led to fun design, individual design and participative design; "emotional revolution" has given rise to multi-sensory design, design with positive feelings, design with negative feelings and aesthetic design; "non-materialistic values" have their correspondence in design as performance and story-telling in design; and "organic worldview" finds its expression in ecological design with a wellness emphasis, and in interactive emotional design.

• Korean Chemical Engineering Research
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• v.52 no.6
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• pp.755-767
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• 2014

Chlorine is one of the most produced and most used non-flammable chemical substances in the world even though its toxicity and high reactivity cause the ozone layer depletion. However, in modern life, it is impossible to live a good life without using Chlorine and its derivatives since they are being used as an typical ingredient in more than 40 percent of the manufactured goods including medicines, detergents, deodorant, fungicides, herbicides, insecticides, and plastic, etc. Even if Chlorine has been handled and distributed in various business (small and medium-sized businesses, water purification plants, distribution company, etc.), there have been few researches about its possible health hazard and transportation risks. Accordingly, the purpose of this paper is to make a detailed assessment of Chlorine-related risks and to model an index of chemicals transportation risks that is adequate for domestic circumstances. The assessment of possible health hazard and transportation risks was made on 13 kinds of hazardous chemicals, including liquid chlorine. This research may be contributed to standardizing the risk assessment of Chlorine and other hazardous chemicals by using an index of transportation risks.

• Journal of Korean Society of Environmental Engineers
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• v.22 no.4
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• pp.689-698
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• 2000

The quality of stormwater runoff has been a major concern in water quality preservation. Characteristics of heavy metals and conventional pollutants in surface runoff from industrial complex, during the first flush, were not completely understood, Generally, separated sewer system is known for their water quality with untreated wastewater during storm events. In this study, the quality and characteristics of surface runoff from the industrial complex were investigated. The target area in the industrial complex catchment was divided 4 sub-areas, and the quality of stormwater runoff from the selected drainage areas was investigated using a grab sampling method. The petro-chemical industry and the junkyard discharged relatively high concentration of conventional pollutants, such as BOD, COD, SS, and TN through the first flush runoff samples. On the other hand, a higher level of heavy metals was found in the first flush runoff from the metal-mechanical industry and the scrap storage yard. For metals, Fe, Zn and Cu were the most prevalent species found in the first flush runoff from all sites for every surface runoff samples, while Pb, As, Cd, Cr and Ni were the least prevalent species and Hg was not found in any sample at any site. These results suggest that the nature of pollutants in surface runoff from the industrial complex was related to the type of industry, and the concentration of pollutants was determinated by the degree of exposed pollutant sources and the characteristic of rainfall events at the sites.

• Journal of Korean Society of Environmental Engineers
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• v.36 no.12
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• pp.821-827
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• 2014

The 4major river project has caused changes in flow and water quality patterns in major rivers in Korea including the Nakdong River where several toxicant release accidents have had occurred. Three dimensional hydrodynamic model, the Environmental Fluid Dynamics Code (EFDC), was applied to evaluate the effect of geomorphological change of the river on the advection and dispersion patterns of a conservative toxic pollutant. A hypothetical scenario was developed using historical data by assuming a toxic release from an upstream location. If there is a toxic release at the Gumi Industrial Complex, the toxic material would be detected after 2.22 and 9.83 days at Chilgok and Gangjung weir, respectively, in the new river system. It was estimated that they took at least 12 times longer than those with the river conditions before the project. Effect of relocation of intake towers for Daegu Metro City to upstream of Gumi City was also evaluated using the developed modeling system. It was observed that hydraulic residence time would be increased due to decreased flow rate and thus due to lowered water level. However, peak concentration differences were found to be about 2% lower in both places due to increased dispersion effect after the relocation.

• Journal of Radiation Industry
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• v.7 no.1
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• pp.7-14
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• 2013

In this study, we investigated the applicability of the photostimulated luminescence (PSL), thermoluminescence (TL) and electron spin resonance (ESR) methods for various foods. The analysed samples consist of 11 items including dried fruits (6 items) and mushrooms (5 items). To compare between non-irradiated and irradiated food, all samples were irradiated with $^{60}Co$ gamma-ray source. In PSL study, the photon counts of all the unirradiated sample were identified negative (lower than 700). The photon counts irradiated (1, 5, 10 kGy) dried date-palm, dried jujube and mushrooms (Auricularia auricula, Phellinus linteus and Sarcodon aspratus) showed positive (higher than 5,000) and the results for the other samples were negative or intermediate (>700 and >5,000). In TL analysis most of items can be applicable to detect irradiated foods because of showing TL ratio above 0.1. The glow curves by TL method were estimated in the range of $150{\sim}250^{\circ}C$ in irradiated samples. In ESR measurements, the intensity of ESR signal (single-line) increased as the dose of irradiation increased. In particular, the specific ESR signals of irradiation-induced radical were detected in dried papaya, dried date-palm, dried banana, dried pineapple and S. aspratus. According to the results, PSL, TL and ESR methods were successfully applied to detect the irradiated foods. It is concluded that PSL, TL and ESR methods are suitable for detection of irradiated samples and a combined method is recommendable for enhancing the reliability of detection results.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.23 no.5
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• pp.393-400
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• 2011

The mass transport is very complicated at the area which has the macro tide and complex geometry such as Gyeonggi bay. Especially, the long period current has a strong influence on the estuarine ecosystem and the long-term distribution of substances. The long period current is caused by several external forcing, whose unique characteristic varies spatially and temporally. The variation characteristics of long period current is analysed and its generation mechanism is studied. The tidal nonlinear constituents such as overtide and compound tide are generated due to nonlinear interaction and it causes mean sea level setup. The tidal wave propagating up into estuary is transformed rapidly by decrease of cross-sectional area and depth. Therefore the mean sea level is getting rise toward upriver. The high and low tide level is similar between down-river(Incheon) and up-river(Ganghwa) during neap tide when the tidal deformation is decreased. The tidal phase difference between two tidal stations causes a periodic fluctuation of sea level difference. The low water level of Ganghwa station during spring tide does not descend under EL(-)2.5 m, but the low water level of Incheon fall down under EL(-)4.0 m. The variation of tidal range and its sea level are increased during spring tide. It is found that the long period current $M_{sf}$ is quite similar to that of sea level difference between the two tidal stations. It means that the sea surface inclination caused by the spatial difference of tidal deformation is important forcing for the generation of long period current.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.32 no.6C
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• pp.267-274
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• 2012

The conventional approach to evaluate the contaminant transport in soils adopts the macro-scale implementation while the pore configuration and network is a dominant factor to determine the fate of contaminant. However, the observation of fate and transport at pore scale may not be readily approachable because of the computational expenses to solve Navier-Stokes equation. We herein present the 2D Lattice-Boltzmann method that enables to assess the local fluid velocity and density efficiently for the case of single phase and multi-components. The solute fate spatio-temperal space is explicitly determined by the advection of fluid flow. Two different types of idealized pore space provides the path of fluid. Also, solute transport, the velocity field and average concentration of solute are computed in steady state. Results show that the pore geometry such as tortuosity mainly affect the solute fate. It highlights the significance of the pore configuration and shape in granular soils and rock discontinuity in spite of the equivalent porosity.

• Analytical Science and Technology
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• v.22 no.2
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• pp.119-127
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• 2009

This study was carried out to investigate the distribution characteristics of persistent organic pollutants in incineration residues at industrial waste incinerators and municipal solid waste incinerators, which were analyzed by the official analytical method for the endocrine disrupting chemicals and the waste. Seven of 12 persistent organic pollutants were quantitatively analyzed by GC-MSD (SIM-mode). Hexachlorobenzene was detected in 21 samples among 44 incineration residues. The level of hexachlorobenzene was 0.132-8.138 ng/g in incineration residues, 0.195-5.765 ng/g in fly ash at industrial waste incinerators, 0.270-1.828 ng/g in bottom ash and 0.154-50.643 ng/g in fly ash at municipal solid waste incinerators, respectively.