• Journal of the Korean Society of Food Science and Nutrition
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• v.41 no.8
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• pp.1057-1065
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• 2012

The effects of volatile flavor extracts of eight different herbal medicines, Juniperus rigida (JR), Saussurea lappa SL), Cnidium officinale (CO), Angelica gigas (AG), Eugenia caryophyllata (EC), Angelica tenuissima (AT), Mentha arvense (MA), and Artemisiae argyi (AA), were investigated on LPS-stimulated inflammation using Raw 264.7 cells. The volatile flavor extracts of CO and AG considerably inhibited LPS-stimulated NO, $PGE_2$, IL-6, and TNF-${\alpha}$ (except AG) production, as well as iNOS expression. Major volatile components of CO were identified as ligustilide and of ${\beta}$-eudesmol as AG by GC-MS analysis. Thus, these results suggest that the volatile extracts of CO and AG may be useful as potential therapeutic agents for inflammation-associated disorders.

• Journal of Life Science
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• v.24 no.7
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• pp.757-761
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• 2014

Olive flounder (Paralichthys olivaceus) is an important aquaculture fish species in Jeju Island, South Korea. Due to the intensification of flounder fish farming, huge amounts of chemical antibiotics are used against several fish diseases. This has many harmful side effects on fish, as well as human consumers. Hence, an alternative to chemical antibiotic agents is needed for disease control. In this study, three strains of rhizobacteria (BRH433-2, TRH415-2, and THJ609-3) were isolated from the rhizosphere of plants. Assays of their antibacterial activity against fish pathogens, such as S. iniae, S. parauberis, V. anguillarum, and E. tarda, were performed with untreated broth culture (without cell separation), supernatant, and precipitated pellets separated by centrifugation. Among these, the cell suspension prepared from the precipitated pellet showed significant antimicrobial activity when compared with that of the untreated broth culture and centrifugal supernatant. These results indicate that the three isolated rhizobacterial strains exhibit antibacterial activity. Analysis of the 16S rDNA sequences of the BRH-433-2, THJ609-3, and TRH415-2 strains showed the highest similarity to Burkholderia gladioli (99.5%), Pseudomonas baetica (97.7%), and P. koreensis and P. baetica (98.4%), respectively. We suggest that the strains hold promise in disease management of fish.

• KSBB Journal
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• v.17 no.6
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• pp.531-536
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• 2002

In this study, we attempted to increase the survivability of bifidobacteria in simulated gastric juices and bile salts after cell entrapment with alginate and various food additives, such as erythritol, isomalt, palatinose, skim milk, xanthan gum, isomalto-oligosaccharide, fructo-oligosaccharide, galacto-oligosaccharide, pectin, and mono-sodium glutamate. Additionaly, the stability of bifidobacteria during storage was investigated by measuring survival rate at different temperatures, i.e. at 4$^{\circ}C$, 25$^{\circ}C$ and -20$^{\circ}C$. Bifidobacteria were immobilized in alginate beads and the survival rate was monitored. It was found that bifidobacieria entrapped with 2.5％, alginate showed the highest survival rate at 12%. After addition of the various protective agents, erythritol(1％) showed the best protective efficiency with a survival rate of 56.0% among the additives tested when exposed to simulated gastric juices for 3 h. Immobilized cells suspended in 5% skim milk and stored at 4$^{\circ}C$ survived significantly more than cells stored at 25$^{\circ}C$ and -20$^{\circ}C$. Consequently, the study shows that the survival rate of bifidobacteria immobilized in combination with 2.5% alginate beads and 1% erythritol may be signifcantly increased in simulated gastric juices and bile salts.

• Korean Journal of Plant Resources
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• v.29 no.1
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• pp.11-19
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• 2016

In this study, we investigated the anti oxidative potential and protective effects of water extract of Leonurus sibiricus L. leaf (LSLW) against ultraviolet B (UVB)-induced oxidative damage in human keratinocytes (HaCaT cells). To evaluate the anti oxidative activity of LSLW, we measured DPPH radical, hydroxyl radical, hydrogen peroxide, superoxide anion scavenging activities, lipid peroxidation inhibition, and reducing power of LSLW. For induction of oxidative stress in HaCaT cells, the cells were irradiated with UVB at 40 mJ/㎠. To investigate the protective effects of LSLW against UVB, we measured cell viability and apoptotic bodies using annexin V staining. LSLW showed anti oxidative activities by scavenging DPPH radical, hydroxyl radical, hydrogen peroxide, superoxide anion and by reducing lipid peroxidation. In addition, LSLW showed high reducing values. The UVB-induced oxidative conditions led to cell apoptosis. However, treatment with LSLW ameliorated oxidative stress conditions, including inhibition of cell death, apoptotic body. Taken together, LSLW exhibited anti oxidative and protective effects against UVB-induced damage in HaCaT cells. Thus, LSLW could be useful for the development of cosmetics for UVB-induced skin aging.

• Journal of Korean Society of Environmental Engineers
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• v.27 no.5
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• pp.519-524
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• 2005

Due to the low C/N ratio of domestic wastewater characteristic, addition of external carbon source for the effective N and P removal is necessary. High organic content of food waste can be used for the external carbon source in biological nutrient removal processes, The applicability of condensate of food waste (CFW), which is produced during the high-rate fermentation process, was examined in membrane bioreactor for the nutrient removal. Under the various operating conditions, nutrient removal efficiencies and membrane fouling characteristics were evaluated using synthetic wastewater. From nitrate utilization rate (NUR) test, denitrification rate was 0.19 g $NO_3-N/g$ VSS/day. With the addition of CFW increased, average removal efficiencies of T-N and T-P could be increased up to 64% and 41%, respectively. Also the optimal retention time was 3 hr/5 hr for anoxic/aerobic reactor. When applied to real sewage, membrane fouling resistance was increased up to 60%, which could be reduced from $10.4{\times}10^{12}m^{-1}$ to $5.9{\times}10^{12}m^{-1}$ with the control of influent suspended solid concentration. In summary, it was suggested that CFW could be used as an economical and effective carbon source for membrane assisted biological N and P removal.

• Korean Journal of Plant Resources
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• v.18 no.1
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• pp.161-168
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• 2005

Tuber yield and content of general component and diosgenin which is a main bioactive property were investigated in order to determine the growth characteristics of round typed yam(Dioscorea opposita L.) and the potential of artificial culture at Suwon, Korea. Tubers of round yam were initiated to form at 60 days after planting and then enlargement of tubers lasted by 160 days after planting. Compared to short typed yam(108g), tuber weight of round yam was higher(127g) on the basis of dry weight at 200 days after planting. In comparison of general component between round yam and short yam, protein of round yam$(3.62\%)$ was higher than short yam$(2.10\%)$. Water content in round yam$(64.5\%)$ was lower in short yam$(79.4\%)$, indicating a higher dry weight ratio of round yam. Hardness of round yam was 2787.6 while short yam showed about two times higher hardness(4946.9). Lightness was higher in round yam(77.4). In tuber extracts analysis, diosgenin content was respectively $3.32\%$ in round yam and $2.61\%$ in short yam.

• Journal of the Korean Society of Food Science and Nutrition
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• v.45 no.11
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• pp.1691-1695
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• 2016

The objective of this study was to determine the antioxidant activities of rice bran extracts by three different extraction methods. Rice bran was extracted by solvent extraction, saponification extraction, and supercritical fluid extraction. The antioxidant activities of the rice bran extracts were determined based on ABTS and DPPH radical scavenging activities, reducing power, and lipid peroxidation inhibitory activity. The unsaponifiable matter (USM) extracted by the saponification method showed higher ABTS (671.7 mg Trolox equivalent antioxidant capacity (TEAC)/g) and DPPH (330.7 mg TEAC/g) radical scavenging activities as well as reducing power ($A_{700}=1.14$) than those of the solvent extract (ME) and supercritical fluid extract (SFE). Inhibitory effect on lipid peroxidation was higher in USM (68.7%) and SFE (75.4%) compared to ME (47.8%). USM indicated relatively higher antioxidant activities compared with those of SFE and ME. These results show that the saponification method for extraction of USM from rice bran extracted was the most effective method for enhancement of antioxidant activity. In addition, these extracts from rice bran could be used as functional ingredients in the food industry.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.7 no.4
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• pp.257-266
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• 2002

To estimate the nitrogen budget and assess the purification function of a tidal flat ecosystem, a field survey was carried out at Hajeon tidal flat in Gomso Bay, the southern part of Byeonsan Peninsula, Chollabuk-do, Korea. A study area of 3.0$\times$4.5 ㎢ was established on the tidal flat and the concentrations of chlorophyll-a, DIN, DON, and TN were measured in the water column during the period of April 17-18, 1999: From the budget analysis, the loss rate of Chl-a was estimated to be -0.05 mg Chl/㎡/hr, which is approximately 7% of that at Issiki tidal flat in Aichi Prefecture, Japan. The lower loss rate of Chl-a in the study area was attributable to the lower standing crop of phytoplankton, the lower temperature that may reduce metabolic rates of biotic components and the lower biomass of macrobenthos in the study area. Over the 13.5 ㎢ of study area, Hajeon tidal flat removed 8.36$\times$10$\^$2/ kg N/day of TN, 5.36$\times$10$\^$3/ kg N/day of PON and 1.62$\times$10$\^$2/ kg N/day of phytoplankton-related PON, showing that the tidal flats may play an important role in removing nitrogen in coastal waters. The removal rate of PON, compared to the removal cost of the existing waste water treatment facilities, indicates that the economic value of the purification function of Hajeon tidal flat (13.5㎢) may be more than that of two large facilities.

• Journal of the Korean Society of Marine Environment & Safety
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• v.24 no.7
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• pp.916-922
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• 2018

Recently, power plant effluent condensers received a Renewable Energy Certificate as components of hydrothermal energy (weighted 1.5 times) as one target item of the Renewable Portfolio Standard (RPS) policy. Accordingly, more attention is being paid to the value of thermal wastewater as a heat source. However, for utilization of thermal wastewater from power plants in high-turbidity areas like the West Sea of Korea, a turbidity reducing system is required to reduce system contamination. In this study, an experimental test was performed over a month on thermal wastewater from power plants located in the West Sea of Korea. It was found that water turbidity was reduced by more than 80 % and that the concentration of organic materials and nutrient salts was partially reduced due to the reduction of floating/drifting materials. To conduct a comparative analysis of the level of contamination of the heat exchanger when thermal wastewater flows in through a turbidity reducing system versus when the condenser effluent flows in directly without passing through the turbidity system, we disassembled and analyzed heat exchangers operated for 30 days. As a result, it was found that the heat exchanger without a turbidity reducing system had a higher level of contamination. Main contaminants (scale) that flowed in to the heat exchanger included minerals such as $SiO_2$, $Na(Si_3Al)O_8$, $CaCO_3$ and NaCl. It was estimated that marine sediment soil flowed in to the heat exchanger because of the high level of turbidity in the water-intake areas.

• Korean Journal of Microbiology
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• v.55 no.3
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• pp.226-233
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• 2019

Ochratoxin A (OTA) that is one of mycotoxins produced mainly by Aspergillus spp. is a common contaminant of stored grains and poses health hazards to human and livestock. The aim of this study is to explore the ability of isolated bacteria Bacillus subtilis AF13 and Streptomyces shenzhenensis YR226 to inhibit growth and OTA production of 3 ochratoxigenic Aspergillus strains. The antifungal activity against mycelial growth and sporulation of Aspergillus strains was examined by coculture with AF13 and YR226 on potato dextrose agar plate. AF13 and YR226 reduced 77.58 and 78.48% of fungal colony radius, respectively, and both strains inhibited fungal sporulation up to 99% in 10 days of incubation. YR226 also reduced more than 91% of spore germination of 3 fungal strains. When Aspergillus strains were cocultured with AF13 or YR226 in yeast extract sucrose medium, mycelial growth and OTA production decreased in all three fungal strains. In particular, AF13 completely inhibited the mycelial growth of A. alutaceus and inhibited its OTA production by 99%, and YR226 also reduced mycelial growth and toxin production up to 99%, respectively. Antimicrobial substances produced by AF13 and YR226 included siderophore, chitinase, protease, ${\beta}$-1,3-glucanase and biosurfactant. These results suggest that AF13 and YR226 can be used in a biological method to prevent valuable crops against mycotoxigenic fungi, and therefore decrease economic damage in agriculture and feed industry.