• Journal of The Korean Society of Grassland and Forage Science
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• v.7 no.1
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• pp.25-30
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• 1987

There are differences in opinion as to whether nitrogen fertilizer should be used when establishing alfalfa (Medicago sativa L.). Various reports show that under a hot environment, rhizobia (Rhizobium meliloti) are not as effective in fixing atmospheric nitrogen as they are under moderate temperatures. It is also believed that the addition of nitrogen fertilizer inhibits nodulation of alfalfa seedlings. A replicated experiment was conducted under controlled environmental conditions at the University of Nevada-Reno, Reno, Nevada, USA, to determine the effects of nitrogen application on seedling growth and nodulation of alfalfa grown in a hot environment. Sterile sand was used as the growing media to which a complete nutrient solution minus nitrogen was applied volumetrically to each pot daily. In addition, half of the pots received NH4-$NO_3$, at the rate of 11.2 kg per ha at seeding and at two and four weeks after planting giving a total nitrogen application rate of 33.6 kg per ha during the seven-week experimental period. Rhizobia inoculant (R-12) consisted of a mixture of strains 171-15a, 1682c and 80 PI 265 of (Rhizobium meliloti). Inoculant was applied to the seeds prior to planting and to the sand media at two and four weeks after seeding. Twenty seeds were planted in pots 14.0 cm in diameter and 11.5 cm deep. Plants were thinned to ten plants per pot after emergence and were grown in a controlled environment chamber with a 16-hour light period. Soil temperature at 6 cm depth ranged from 17.4^{\circ}C.$ to 31.1^{\circ}C.$ and had a daily mean of 26.5^{\circ}C.$. Plants were harvested at weekly intervals for seven weeks. Root, shoot and total length, dry weight, volume and number of nodules per plant were determined. Root, shoot, and total length were greater in seedlings grown in soil where nigrogen was applied than that grown in soil to which no nitrogen was applied. The average size of the seedlings as determined by volume and weight was more than two times greater where plants were fertilized with nitrogen. Nodule number per seedling was also greater when nitrogen was applied compared to those which received no nitrogen. The differences were greater as the plants became older. The rhizobia did not fix enough nitrogen for adequate growth of seedlings. This is probably due to high temperature growing conditions that caused the rhizobia to become relatively ineffective as compared to cooler growing conditions. Data suggests it would be desirable to apply nitrogen at seeding when alfalfa is established under hot conditions that occur in mid- or late summer.

• Journal of Food Hygiene and Safety
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• v.28 no.2
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• pp.188-193
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• 2013

Anatoxin-a, saxitoxin and neosaxitoxin are produced by Aphanizomenon flos-aquae that is a sort of the cyanobacteria phylum. Therefore, it is not permitted for food materials in Korea. Traditionally, the classification of cyanobacteria has been based on morphological characters such as trichome width, cell size, division planes, shape, and the presence of character such as gas vacuole. But, some diagnostic features, such as gas vacuole or akinetes, can show variation with different environmental or growth conditions and even be lost during cultivation. Therefore, we developed detection method for functional foods containing Aph. flos-aquae by PCR. To design the primer, 16S rRNA region of Aph. flos-aquae, Spirulina laxissima, and Spirulina spp. registered in the GeneBank (www.ncbi.nlm.nih.gov) have been used and for comparative analysis, BioEdit ver. 7.0.9.0. was used. As a result, we was design AFA-F1/AFA-R1 (363 bp) primer for the differentiation Aph. flos-aquae from chlorella, spirulina, green tea, and spinach. Also, it could be distinguished chlorella and spirulina products those are made to contain 1% Aph. flos-aquae.

• Journal of the Korea Safety Management & Science
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• v.22 no.3
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• pp.1-8
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• 2020

The desulfurizers facility is cylindrical shape. To operate properly it needs nozzles cleaned, get rid of lime adhesion and sludge, repair the wear and corrosion of facility regularly. For this purpose, workers shall access the ceiling or vertical wall at high place. Ordinary scaffoldings such as steel pipe scaffolding or system scaffolding have been using so that workers can access them. With these ordinary scaffoldings, openings around cylindrical wall are inevitable which make workers can expose always to the risk of falling. The purpose of this study is to develop customized scaffolding to minimize the openings to prevent workers form falling during maintenance it. It consists of a hexagonal central tower and six trapezoidal outer towers. And the bracing among the towers have connected each other for self-standing and for maintaining the structure of towers. Span decks, the circumference footstools, steps, etc. are laid on each floor. The safety is reviewed by structural analysis and performance test. With this study, openings each floor of this scaffold are removed. The gap between the cylindrical wall and the edge of the work stage is approximately 100 mm. Therefore, we expect that workers can work safely and efficiently.

• Korean Journal of Soil Science and Fertilizer
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• v.11 no.2
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• pp.75-80
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• 1979

Ammonium desorption from 16 soils treated with $(NH_4)_2HPO_4$ solution (2000 ppm $NH_4$) was investigated by seven extractions with 0.01M $CaCl_2$. 1. There were 2 to 4 steps alternately appeared with fast and slow mode. 2. Desorption equation, log y=b-ax where y is desorption amount, b a constant indicating adsorption maximum, a retention constant, and x extraction number, was held for each step. 3. Desorption rate (100${\times}$desorption / adsorption) was 65% for the average of 15 soils, maximum 87% in Gimcheon series, minimum 32% in Samgag series. Yongho series (a peat soil) showed 156% indicating the release of large quantity of indigenous soil ammonium. 4. Desorption rate was negatively correlated with initial adsorption and in this relation the tested soils were classified into 3 groups. 5. The cumulative desorption curve was approaching almost to maximum in all tested soils with seven extractions. The final retention amount, ranged from 25% of CEC (Gimhae series) to 502% (Samgag Series). 6. Amount and rate of desorption did not have any significant relation with Langmuir adsorption maxima of ammonium, CEC and contents of clay, available phosphorus and organic matter. 7. The above results may indicate that adsorption and desorption of ammonium is closely related with iron, aluminum silicate and adsorption and desorption characteristics of accompanied anions.

• Korean Journal of Soil Science and Fertilizer
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• v.34 no.5
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• pp.342-349
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• 2001

This study was carried out to classify and evaluate the land cover map using Landsat TM data in Pyeongtaeg City. DGPS data, aerial photography, topographical map were used for selection the training sets and accuracy assessment. The overall accuracy and Kappa coefficient of the land cover classification map(using supervised classification with 13 classes) with Landsat TM data(16 June. 1997) were respectively, 86.8%, 85.4%, but the user's accuracy of urban/village and vinyl-house was below 60%, and the producer's accuracy of read and vinyl-house below 70%. Maybe it was caused the spectral reflectance characteristics, heterogeneity and small distribution area on the artificial things such as urban/village, vinyl_house and road, etc. And then, the agricultural land cover classification system using remote sensing data in Korea was to classify level I and II. Level I consisted of 5 classes such as agricultural land, forest land, water, barren land, urban and built-up land.

• Proceedings of the Korea Water Resources Association Conference
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• 2007.05a
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• pp.97-105
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• 2007

수문학적 토양유형은 복잡하게 세분되어 있는 토양의 종류를 수문학적인 목적에 따라 단순화하기 위해 만든 것으로 미농무성 토양보전국에서 고안한 개념이다. 우리나라 토양을 이 방법으로 구분하고자 하는 몇번의 시도가 있으며 그 중 대표적인 것이 정 등(1995)이 분류한 수문학적 토양군이다. 이는 토양의 침투 투수특성에 대한 실측자료가 부족한 우리나라의 실정을 감안하여 토성속(textural family), 배수등급(drainage class), 불투수층(impermeable layer), 투수성(permeability)의 네가지 토양특성을 분류특성으로 하여 각각에 1-4점 범위로 점수를 매긴 후 합산한 점수를 기준으로 수문학적 토양유형을 분류하는 것이다. 최근에는 토양의 한계침투속도에 따라 수문학적 토양유형을 분류하고자 하는 시도가 있으며 본 논문에서는 새로운 방법으로 분류할 때 기존의 방법과 어떠한 차이가 있는지 비교하고자 하였다. 정 등(1995)의 분류방법은 개념상 몇가지 문제점을 안고 있다. 먼저 토양의 수리특성은 같은 토성속이라 하여도 토양생성 과정과 토지이용 방법에 따라 그 차이가 매우 큼에도 불구하고 이에 대해 고려하지 못하였으며 다음으로 지표유거가 많아 배수가 양호한 토양의 강우 유출을 과소평가한다. 또한 얕은 토심에 존재하는 불투수층이 존재하는 경우 토양의 수리특성에 관계없이 적은 양의 강우에도 유출이 발생하므로 별도의 제한인자로 간주하여야 한다. 토양의 한계 침투속도를 이용한 분류방법은 이러한 문제점을 상당 부분 개선할 수 있다. 토양의 한계침투속도를 산정하기 위해 현장에서 지표 한계침투속도와 투수속도를 측정하였으며 이 자료를 확장하여 해석하기 위해 입자특성을 이용한 Pedo Transfer Function을 개발하였다. 토심 50 cm 포화시 토양 투수성을 한계 침투속도로 가정하였으며 50 cm 이내에 암반층과 지하수위가 존재할 경우 투수성에 관계없이 D유형으로 분류하였다. 새로운 방법으로 분류한 결과 기존의 분류와 몇가지 차이점이 발견되었다. 가장 큰 차이는 대부분의 논토양이 느린 한계침투속도의 영향으로 D유형에 속한 것이다. 산림토양과 밭토양은 기존 방법과 마찬가지로 A, B유형이 많았으며 암반층을 고려하기 전에는 기존 분류에 비해 강우 유출 가능성이 적은 쪽으로 평가되었다. 그러나 암반층이 존재하는 토양을 고려한 결과 A 또는 B 유형에 속하던 상당수의 산림토양이 새로운 분류에서 D유형으로 분류되었다. 지표 유거가 많아 배수등급이 매우양호로 분류되던 토양은 정 등(1995)의 분류와 비교하여 대부분 강우 유출 가능성이 큰 쪽으로 조정되었다. 새로운 수문학적 토양유형을 이용할 경우 낮은 토심에서 암반층이 발견되는 산림토양이 분포한 유역이나 산림, 밭 등에 식질 토양이 많이 분포하는 유역에서는 기존의 방법을 이용하는 것보다 강우 유출량이 높게 평가될 것으로 판단된다. 앞으로 강우 유출량 실측자료와의 비교를 통해 지속적인 보정을 하여야 할 것이며 특히 불투수층의 존재시 일괄적으로 D유형으로 분류된 토양의 경우 깊이에 따라 C 또는 D 유형으로 세분하여 조정할 필요가 있다.

• Proceedings of the Korea Water Resources Association Conference
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• 2007.05a
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• pp.84-88
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• 2007

우리나라의 토양조사는 미농무성(USDA)의 분류방법에 따라 개략토양조사($'65{\sim}'67$년), 정밀토양조사($'68{\sim}'79$년), 논토양배양사업 10개년계획($'80{\sim}'90$년), 그리고 밭토양세부정밀토양조사($'95{\sim}'99$년) 등을 실시하였다. 현지에서의 토양조사는 지형, 배수등급, 토성, 자갈함량, 유효토심, 경사 등 현지의 지형지물 및 환경을 통하여 많은 정보를 얻을 수 있으며, 이를 통하여 전국에 대한 토양조사 결과, 토양통 (심토의 특성이 유사한 토양) 390개, 토양구(표토의 토성을 세분) 536개, 토양상(경사도 및 침식등급을 세분) 1,321개로 분류되어 있다. 우리나라 토양형성 발달에 미친 기후인자는 습윤권역(Udic moisture regime)과 토양온도는 mesic과 일부 남해안과 제주지역의 thermic인 토양온도(Soil moisture regime)였으며, 식생인자는 높은 지역의 경우에는 산림이 낮은 저지대 평탄지는 자연초지가 대부분이다. 지형인자는 동고서저지형으로 동부는 산악지, 서부는 경사가 완만한 구릉지며, 한강 등 대부분이 서남방향으로 흐르는 지형이다. 모재는 다양하나 70%이상이 화강암, 화강편마암이 주를 이루고 있었다. 시간인자의 경우에는 기간이 매우 오래되었으나, 지형이 복잡하여 토양발달이 약한 Inceptisol과 층위발달이 없는 Entisol이 대부분이며, 다음으로 Ultisol과 Alfisol이 뒤를 이었다. 일부 Andisol, Mollisol, Histosol이 존재하였다. 우리나라의 토양분류는 Soil Taxonomy에 의해 분류되었는데, 이것은 토양의 단면내에 존재하는 감식층위(Diagnostic Horizon)의 유무 및 종류에 따른 분류이다. 토양분류 결과 최고 상위분류 단위인 목(order)이 7개, 아목(suborder) 14개, 대군(great group) 27개, 아군(sub group) 67개, 속(family) 161개로 분류되었으며, 가장 저차단위인 통(series)이 390개 분류되었다. 또한 작물이용측면에서 실용적 분류를 실시하였는데, 논토양의 경우 보통논, 미숙논, 사질논, 습논, 염해논, 특이산성논으로 분류할 경우 각각의 분포면적이 31.9%, 23.0%, 31.9%, 9.1%, 3.9%, 0.2%이었다. 밭토양의 경우에도 보통밭, 사질밭, 미숙밭, 중점밭, 고원밭, 화산회밭으로 6개 유형으로 분류할 경우 각각의 분포면적은 41.9%, 23.3%, 17.5%, 13.9%, 1.1. 2.2% 이었다. 도시화 및 도로확대 등 다양한 토지이용 및 지형개변으로 과거의 토양정보가 많이 변경되었다. 그래서, 앞으로는 인공위성자료 및 항공사진을 이용하여 빠르고 쉽게 활용할 수 있는 토양조사 방법개발과 기 구축된 토양도의 수정, 보완 작업이 필요한 절실히 요구되고 있는 현실이다.

• The Korean Journal of Mycology
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• v.16 no.3
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• pp.151-156
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• 1988

A leaf anthracnose disease of pepper(Capsicum annuum L.) seedling caused by Colletotrichum coccodes was found in fields of Chungnam District for the first time in 1988. Initial symptom of the disease was spotting on leaves and stems of the seedlings. The spots became sunken and gradually increased in size and coalesced to form bigger irregular spots and blights on the leaves. In severely affected fields, many pepper seedlings were defoliated and lodged. Field survey revealed that the infection rate of anthracnose ranged form 15.3% to 92.3%. Conidia from lesions on seedlings were hyaline, aseptate, straight, cylindrical with obtuse ends, $115-25(av.\;19.\;5){\times}2.5-4.8{\mu}m(av.\;3.3{\mu}m)$ and setaes were straightly extended above the acervuli, $41.3-136.3\;{\um}m(av.\;85.0{\mu}m)$ long. The fungus produced abundant sclerotia on PDA when it was cultured under darkness. In the inoculation experiments, the fungus was found to infect pepper seedlings and fruits, and fruits of tomato and egg plant.

• Journal of The Korean Society of Grassland and Forage Science
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• v.6 no.3
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• pp.151-156
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• 1986

There are differences in recommendations in the USA as to whether nitrogen fertilizer should be applied when establishing alfalfa (Medicago sativa L). The reason for not applying nitrogen is because some researchers found the addition of nitrogen reduced nodulation of alfalfa plants. A replicated experiment was conducted under controlled environmental conditions at the University of Nevada-Reno, Reno, Nevada, USA, to determine the effects of nitrogen application on seedling growth and nodulation of alfalfa when grown in a cool environment. A sterile sand was used in the growing media to which a complete nutrient solution minus nitrogen was applied volumetrically to each pot daily. Half of the pots received $NH_4NO_3$, at the rate of 11.2 kg/ha, at seeding and two and four weeks after planting, giving a total nitrogen application rate of 33.6 kg/ha. Rhizobia inoculant (R-12) consisted of a mixture of strains 171-15a. 1682c and 80 PI 265 of Rhizobium meliloti. Inoculant was applied to the seeds prior to planting and to the sand media at two and four weeks after seeding. Twenty seeds were planted in pots 14.0 cm in diameter and 11.5 cm deep. Seedlings were thinned after emergence to ten plants per pot. They were grown in a controlled environment chamber with a 16-hour light period. Soil temperatures at 6 cm depth ranged from $5.7^{\circ}C\;to\;21.5^{\circ}C$ and had a daily mean of $16.2^{\circ}C$ Plants were harvested at weekly intervals for seven weeks at which time root, shoot and total length, dry weight, volume and number of nodules per plant were determined. Root, shoot and total length were not affected by nitrogen fertilizer. However, application of nitrogen increased the size of the seedlings as determined by dry weight and volume when compared to plants which were not fertilized. This indicates that rhizobia did not fix enough atmospheric nitrogen to promote good growth. Nitrogen application resulted in significantly more nodules per plant. The effect of nitrogen fertilizer became more apparent as the plant became older. Results of this experiment show there are benefits from applying nitrogen at a low rate when establishing alfalfa under a cool environment.

• Journal of KIISE:Software and Applications
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• v.34 no.3
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• pp.226-234
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• 2007

In this paper, we develop a speech recognition system using a throat microphone. The use of this kind of microphone minimizes the impact of environmental noise. However, because of the absence of high frequencies and the partially loss of formant frequencies, previous systems developed with those devices have shown a lower recognition rate than systems which use standard microphone signals. This problem has led to researchers using throat microphone signals as supplementary data sources supporting standard microphone signals. In this paper, we present a high performance ASR system which we developed using only a throat microphone by taking advantage of Korean Phonological Feature Theory and a detailed throat signal analysis. Analyzing the spectrum and the result of FFT of the throat microphone signal, we find that the conventional MFCC feature vector that uses a critical pass filter does not characterize the throat microphone signals well. We also describe the conditions of the feature extraction algorithm which make it best suited for throat microphone signal analysis. The conditions involve (1) a sensitive band-pass filter and (2) use of feature vector which is suitable for voice/non-voice classification. We experimentally show that the ZCPA algorithm designed to meet these conditions improves the recognizer's performance by approximately 16%. And we find that an additional noise-canceling algorithm such as RAST A results in 2% more performance improvement.