• 산림경영
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• s.143
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• pp.46-48
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• 2000

산림청 임업연구원(원장 노의래)은 최근 침엽수종인 소나무, 낙엽송 및 리기테다소나무 등 체세포배에 의한 복제 묘목을 대량생산하는 생물공학적 기술을 개발하는데 성공하였다. 지금까지 임업에서는 접목이나 꺾꽂이 등의 무성증식방법으로 특정형질을 가진 복제묘목을 생산하여 왔다. 특히 이러한 방법은 유실수, 포플러 등에 오랫동안 적용되어 왔으나 최근에는 체세포배에 의한 복제기술이 주로 활엽수 등에서 성공한 바 있다. 이번에 개발한 체세포배에 의한 복제기술은 내년부터 솔잎혹파리에 강한 내충성 소나무, 형질이 월등히 우수한 낙엽송 및 리기테다소나무의 대량생산에 적용하여 단기간내에 솔잎혹파리 내충성 소나무 복제묘목을 보급함으로써 솔잎혹파리 피해가 없는 우량소나무 조림이 가능할 것으로 기대하고 있다. 현재 캐나다 및 뉴질랜드와 같은 임업 선진국가에서는 체세포배에 의한 복제기술을 이용하여 새로운 우수 품종을 산업적인 규모로 대량 생산하고 있으나, 우리나라 소나무와 같이 2엽송(二葉松)인 경우 체세포배에 의한 복제 식물체를 생산한 것은 세계적으로 드문 일이다. 이번 연구 결과는 유전자조작 등의 첨단 생물공학 기법을 나무에 적용하기 위한 필수적인 전(前)단계로서 침엽수 육종에서 새로운 전기를 마련하였다고 볼 수 있으며 특히 임업도 농작물과 같이 특수한 품종을 육성할 수 있는 길을 터놓아 산림자원화와 산주소득증대에 크게 기여할 수 있을 것이다.

• The Korean Journal of Ecology
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• v.25 no.5
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• pp.349-352
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• 2002

The effects of Mn on growth and nutrient status of Pinus densiflora seedlings grown in a nutrient culture solution were investigated. Mn concentrations was added as manganese chloride at 0, 30 and 60ppm to the nutrient culture solution. The 2-year-old seedlings were transplanted into the solution maintained at pH 4.0, and grown for 90 days in a greenhouse. The Mn treatment induced a significant reduction in the dry weight growth of the seedlings. The relative growth rate(RGR) and net assimilation rate(NAR) of the seedlings decreased with increasing Mn concentrations in the nutrient culture solutions. For the nutrient status of the seedlings, Ca and Mg content in trunk and root was least in 60ppm Mn treatment, and Mn content in needle was about 3 times more than in root. Also the net photosynthetic rate of the seedlings was significantly lower both in 30ppm and 60ppm Mn treatment compared to them in 0ppm. This result suggests that the reductions in the RGR and NAR of the seedlings may be resulted from the inhibition of net photosynthesis by the mixed effect of lower nutrient uptake of roots and excess accumulation of Mn in needle.

• Korean Journal of Environmental Agriculture
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• v.17 no.4
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• pp.358-361
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• 1998

This study was carried out to develop a short term hardening method of tree seedlings of Rhus chinensis Mill., Evodia daniellii Hemsley and Parthenocissus tricuspidata(Sieb. et Zucc.) Planck for afforestation on a concave and a crack of cut-rock slope. The seedlings were grown in a cylinder shaped pot made of polyvinyl net with the soil media of peatmoss, vermiculite, clay, compost, fertilizer, and absorbant(40:25:19:15:1:0.1, v:v). They were cultivated in a greenhouse for four months and in field condition for two months. During the last three months of the growing period the seedlings were hardened by periodic desiccation and irrigation in 4 to 10 days interval. The hardened seedlings showed lower leaf water potential, higher leaf osmotic pressure, and lower T/R ratio than those before the hardening. The hardened seedlings survived well on the soil medium in the concave of cut-rock slope.

• Journal of Korean Society of Forest Science
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• v.99 no.6
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• pp.843-848
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• 2010

The effects of soil acidification on the photosynthetic activity of Quercus acutissima seedlings were investigated. We measured the growth and photosynthetic activity of the seedlings in relation to soil acidification. The dry weights of the seedlings were reduced according to the amount of $H^+$ in the soil. The concentrations of Al in needles at the 90 meq $H^+$ were significantly higher than those at the control. The contents of chlorophyll in needles at 90 meq $H^+$ were significantly lower than those at the control. The net photosynthetic rates of the seedlings in the acidified soil were reduced by increasing the amount of $H^+$ to the soil. The carboxylation efficiencies(CE) of photosynthesis were reduced in the seedlings grown in the acidified soil. These results suggested that the soil acidification induced the inhibition of photochemical reactions and $CO_2$ fixation of photosynthesis.

• Journal of Korean Society of Forest Science
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• v.96 no.3
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• pp.307-316
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• 2007

Yellow poplar (Liriodendron tulipifera L.) has low germination rate relatively other species, so the seedling production of Yellow poplar is a hard task. Accordingly this study was conducted to determine the optimal germination conditions for healthy seedling production and to promote survival rate after afforestation. Gemination percentage was examined at different media and seed covering materials using planting flats in the greenhouse. The best germination percentage was observed in sand for media and compound soil for covering materials. But it was time to transplant, seedlings became a poor character (i.e. height, root length, number of root, dry weight) in sand for media. In order to produce healthy seedlings, each different medium was compounded with TKS-2 (this is a gardening bed soil.) in the ratio 1:1 (v/v.), and compared two conditions. Quality of seedling was better than not mixed TKS-2 into each medium. Transplanting seedlings from greenhouse to nursery grew up rapidly 2 months later (early in August~early in October). Growth amount during two months corresponded to 85.6% and 71.3% in total growth amount of height and diameter at root collar, respectively. In the case of the competition-density effect on yellow-poplar seedlings, direct seedling produced the maximum 35 standard seedlings above 8 mm of root collar diameter per $m^2$, while transplanting seedling produced the maximum 64 standard seedlings per $m^2$. And produced seedlings of two way were significantly different rootlet while axial root and lateral root was not significantly different.

• Journal of Korea Foresty Energy
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• v.24 no.1
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• pp.13-27
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• 2005

In order to design new fertilizers for forests, seedlings of Pinus densiflora, Larix leptolepis Betula platyphylla var. japonica, and FQuercus acutissima were treated with different fertilizers, and nutrient concentrations in seedling components were analyzed. In general, nitrogen (N) and phosphorus (P) concentrations in seedling components increased after N and P application. Potassium (K) concentration in seedling components of Pinus densiflora and Quercus acutissima increased after N, P and K application. However, there were no significant changes in calcium (Ca), sodium (Na) and magnesium (Mg) concentrations. Nitrogen and P concentrations in seedling components increased with the amount of solid combination fertilizer and UF combination fertilizer. Potassium concentration for Pinus densiflora and Na and Ca concentrations for Larix leptolepis also increased with the amount of combination fertilizers. However, nutrient concentrations in seedling components showed no significant changes with different combination fertilizers.

• Journal of Korean Society of Forest Science
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• v.97 no.3
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• pp.266-273
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• 2008

The effects of soil acidification on the seedling growth and nutrition of Pinus densiflora and Quercus acutissima were investigated. The relationship between the seedling growth and molar (Ca+Mg+K)/Al ratio of in soil solution was examined. The results suggested that growth inhibition of seedling Pinus densiflora and Quercus acutissima was due to the low pH of soil solution, which was followed by leach of Al into soil solution, and decrease of essential elements, such as Ca in aerial pant of the seeding caused by the increase of Al concentration in subterranean pant of the seedlings. The level of growth inhibition was determined not only by Al concentration, but also by the balance of inorganic elements, Al, Ca, Mg and K. The growths of two species in total dry weight were clearly inhibited when molar (Ca+Mg+K)/Al ratio of the soil was lower than 6.0. The growth in dry weight, in the condition of the molar ratio was 0.8, was decreased 60% or 50% for the seedling of Pinus densiflora or Quercus acutissima respectively. It was concluded that the molar (Ca+Mg+K)/ Al ratio could be an important index for evaluation of the effects of soil acidification, due to acid deposition such as acid rain, on growth of trees and nutrition. And it might be a more useful indicator for evaluation of critical load of acid deposition on forest ecosystems.

• Journal of Korean Society of Forest Science
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• v.90 no.1
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• pp.74-82
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• 2001

The effects of Al and Mn concentration on dry weight growth, nutrient status and gas exchange rates of 2-Year-old Japanese red pine(Pinus densiflora) seedlings grown in a nutrient culture solution were investigated. Al was added as aluminum chloride at 0, 10, 30 or 60ppm, and Mn was added as manganese chloride at 0, 30 or 60ppm to the nutrient culture solution. The pH of the solution was maintained at 4.0 by adding HCl or NaOH solution. The seedlings were transplanted into the nutrient culture solution, then they were grown in a greenhouse for 90 days. The interactive effects of Al and Mn on the dry weight growth of the seedlings were not significant. There were a main effect of Al or Mn on the dry weight growth and element concentrations of the seedlings. The treatment with Al of ${\geq}10ppm$ or that with Mn of 60ppm induced a significant reduction in the dry weight growth, which indicates that the effect of Al is stronger than that of Mn. The chlorophyll content of needles was not affected by Al treatment, but was significantly reduced by treatment with Mn of 60ppm. Furthermore, the treatment with Al of 60ppm or that with Mn of ${\geq}30ppm$ caused a significant reduction in the dark respiration rate of the roots. The net photosynthetic rate of the seedlings reduced with increasing the concentration of Al or Mn in the nutrient culture solution, which suggests that Al or Mn induced reductions in the relative growth rate(RGR) and net assimilation rate(NAR) of the seedlings were mainly due to the decrease of net photosynthesis.

• The Hankook-Saengyark Bo
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• no.257
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• pp.5-5
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• 2003

헛개나무 묘목 대량생산 - 구기자 수확기 개발 생산비 절간(손수확 보다 작업능률 5배 향상) - 가시오갈피 성묘율 높인다(실생묘 $1m^2$당 66포기 심어) - 흑복분자 신품종 개발 - 절화 용담 12월말$\~$1월초 파종(남원서 평가회 $\cdots$ 심는거리 40*30cm)

• Journal of Korean Society of Forest Science
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• v.109 no.2
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• pp.243-247
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• 2020

This study investigates bench grafting (BG) and field grafting (FG) in walnut by comparing the graft take rate, grade ratio, and simple earning rate using a conventional method. The mean grafting-take rate of the BG was 30.3% while that of FG was 77.1%. The quality of grafted seedlings is classified as good, fair, and poor. The quality grade ratio of FG seedlings is 3:4:3 (good:fair:poor) while the grade ratio of BG seedlings is 2:3:5. Field grafting resulted in 20% more good and fair seedlings than the BG method. As a result of calculating the earning rate with the sales income versus the input cost, FG has 1.5 times higher earning rate than BG. In summary, FG could be an alternative to conventional epicotyl grafting because it produced high-quality seedlings and increased productivity due to its high graft-take rate and earning rate.