• Korean Journal of Food Science and Technology
• /
• v.33 no.4
• /
• pp.401-408
• /
• 2001

The ethanol extract and n-hexane fraction of Commiphora molmol Engl. showed minimum inhibitory concentration of 50 ppm and 25 ppm, respectively, on 5 strains of Listeria monocytogenes at $32^{\circ}C$. The purified substance, C3-3-2 fraction, was isolated by silica gel column and preparative thin layer chromatography from n-hexane fraction of Commiphora molmol Engl. The C3-3-2 fraction showed a strong bactericidal activity on 5 strains of L. monocytogenes at the concentration of 10 ppm in tryptic soy broth medium. At that concentration, the viable count was reduced $5{\sim}6$ log cycle from initial cell number. The n-hexane fraction of Commiphora molmol Engl. showed strong growth inhibition at the concentration of 25 ppm on Bacillus cereus and Staphylococcus aureus, at 50 ppm in broth on Salmonella enteritidis, and at 500 ppm on Vibrio parahaemolyticus. The purified antimicrobial substance, the C3-3-2 fraction, was identified as m-nonylphenol by on the basis of the $^1H-,\;^{13}C-NMR$ and EI/MS data. For the application test, the C3-3-2 fraction which was purely isolated from Commiphora molmol Engl. at 100 ppm were applied to minced Alaska pollack and ground beef at $32^{\circ}C$ and $5^{\circ}C$. The antimicrobial substances did not reduce L. monocytogenes ATCC 19113 at $32^{\circ}C$, while they reduced L. monocytogenes ATCC 19113 in viable number at $5^{\circ}C$. However, the antimicrobial effect of C3-3-2 fraction in food system was lower than that of broth condition.

• Analytical Science and Technology
• /
• v.13 no.5
• /
• pp.630-635
• /
• 2000

The concentration of neurotransmitters in rat brain was determined by HPLC-ECD (electrochemical detection) method and the effects of methanol extracts of some crude drugs, such as Polygala Radix, Myristicae Semen, Zizyphi Semen, Acori graminei Rhizoma, Visci Herba, Liriopsis Tuber, Myrrha on the concentration of neurotransmitters in the ethanol-treated rat brain were investigated. By the administration of ethanol, dopamine (DA), 3, 4-dihydroxyphenyl acetic acid (DOPAC) and serotonin (5-HT) levels in frontal cortex and 5-HT level in hippocampus were significantly increased compared with the neurotransmitter levels in the brain of saline-treated rats. The ${\gamma}$-aminobutyric acid (GABA) level in frontal cortex was decreased by the same treatment. There was a tendency that the DA level in frontal cortex and striatum of ethanol-treated rats were increased by the administration of crude drug extracts. Especially, Myrrha and Visci Herba significantly increased the DA level of frontal cortex in ethanol-treated rats, while they significantly decreased the 5-HT level in the same region of the brain. GABA level in striatum of ethanol-treated rats was significantly decreased by Myristicae Semen, Visci Herba and Myrrha. These results suggest that the tested crude drug extracts have selective interaction with neurotransmitters in specified region of central nervous system.

• Journal of Periodontal and Implant Science
• /
• v.21 no.1
• /
• pp.173.1-173.1
• /
• 1991

• The Journal of Korean Medicine
• /
• v.21 no.2
• /
• pp.79-86
• /
• 2000

Objectives and Methods : In order to elucidate toxic mechanism of myocardial damage and protective effect of myrrha water extract against cytotoxic effect of xanthine oxidase/hypoxanthine(XO/HX), cardioprotective effect of myrrha water extract was examined by MTT assay, LDH (Lactate Dehydrogenase) activity and heart beating rate after cultured myocardial cells derived from neonatal mouse were treated with various concentration of XO/HX, a free radical. Results : XO/HX induced a decrease of cell viability, an increase in the amount of LDH, and a decrease of heart beating rate on cultured myocardial cells in a dose-dependent manner. In cardioprotective effect of myrrha water extract, it showed a decrease in the amount of LDH and an increase of heart beating rate on cultured myocardial cells damaged by XO/HX. Conclusions : From the above results, it is suggested that XO/HX showed toxic effect in cultured myocardial cells derived from neonatal mouse and that myrrha water extract is very effective in the prevention of XO/HX-induced cardiotoxicity.

• Journal of Oral Medicine and Pain
• /
• v.38 no.4
• /
• pp.299-312
• /
• 2013

Even though there exist a lot of study about antibacterial effects and reactions of extracted materials from plant, few study exist about oral pathogenic bacteria. Therefore we tried to recognize about the suppression effect to the periodontal pathogenic bacteria and halitosis, when add some kinds of plant extracted materials, myrrh, rhatany, chamomolilla to saliva. We used Crude drug : Myrrh tincture (100mg/ml), Ratanhia tincture (100mg/ml), Chamomile tincture(100mg/ml). We inspected about the cariogenic bateriae, S. mutans GS5 and S. sobrinus 6715, periodontal pathogenic bacteria, P. gingivalis 2561, P. intermedia ATCC 25611, Candida albicans ATCC 18804, and E. feacalis ATCC 4083, then the result follow. The plant extracted material, myrrh, rhatany, chamomomilla, which have convergence effect, bacteriocidal effect and anti-inflammation effect, show an antibacterial effect and reaction to the oral pathogenic bacteria. And with treating rhatany that have the most strong antibacterial effect, through transmission electron microscopy we could see a severe morphologic change of bacteria. This means with the plant extracted material, we can suppress the oral harmful bacteria and prevent periodontal diseases, caries, halitosis and oral inflammations. And within the future studies for the improvement of oral hygiene, our result might be a clinical evidence.

• The Journal of Internal Korean Medicine
• /
• v.27 no.3
• /
• pp.715-724
• /
• 2006

Objective : To identify the inhibitory effects of Commiphora Myrrha (CM) on monosodium urate (MSU)-induced gout model in rats. Materials and Methods: After pretreatment with CM-I (125mg/kg) or CM-II (50mg/kg) for 7 days followed by ones injection of MSU solution. the various indicators related to gout were measured on hematological and serum level including joint inflammation, Also, it was studied whether FBM directly inhibits the activity of xanthine oxidase in vitro. Results : As a result of this study, CM didn't show cytotoxicity in Jurkat cells, but it showed significant inhibition of activity of xanthine oxidase in vitro. CM slightly inhibited joint inflammation induced by MSU though not with statistical significance. CM partially decreased MSU-induced albumin, globulin, AST, ALT, BUN, creatinine. WBC, platelet count and ESR level and significantly decreased MSU-induced uric acid in serum. Conclusion : These results suggest that CM has therapeutic effects that are applicable to prevention and treatment of gout, and should be further investigated.

• Korean Journal of Pharmacognosy
• /
• v.46 no.3
• /
• pp.243-249
• /
• 2015

Myrrh, Angelicae Radix, and Schisandrae Fructus have been used to treat diverse diseases including neurological disorder in the traditional Korean medicine. In the present study, we investigated the ameliorating effects of HPM-1, a combined extract of Myrrh, Angelicae Radix and Schisandrae Fructus, on scopolamine-induced memory impairments in mice. First, we assessed HPM-1, HPM-2 and HPM-3, in which Myrrh was extracted with water, 30% ethanol, and 30% ethanol/3% vinegar, respectively. The oral administration of HPM-1, HPM-2, or HPM-3 significantly reversed scopolamine-induced reduction of spontaneous alternation in the Y-maze task. In the passive avoidance task, HPM-1 or HPM-3 restored the decreased latency time of the retention trial by scopolamine treatment, but in terms of efficacy, HPM-1 showed more beneficial effects than HPM-3. In addition, HPM-1 administration reversed scopolamine-induced reduction of spontaneous alternation in the Y-maze task and scopolamine-decreased latency time in the passive avoidance in a dose-dependent manner. These results suggest that HPM-1 has the therapeutic potential in memory impairments.

• The Korea Journal of Herbology
• /
• v.31 no.4
• /
• pp.11-18
• /
• 2016

Objectives : The objective of this study was to investigate the effect of Myrrh 80% ethanol extract on adipocyte differentiation and adipogenesis in 3T3-L1 cell.Methods : Myrrh was prepared by extracting with 80% ethanol. Cell viability was assessed by MTT assay using 3T3-L1 cells. Anti-obesity activity was measured in lipid droplets and triglyceride (TG) accumulation in 3T3-L1 cells. We also analyzed the expression of C/EBPβ, C/EBPα, PPARγ, SREBP1c, and aP2 by reverse transcriptase polymerase chain reaction (RT-PCR). In addition, we observed the production of fatty acid, acetyl-CoA carboxylase and Oil-red O stainingResults : No cytotoxicity from Myrrh 80% ethanol extracts was observed at the concentration of 1, 10, 100 (㎍/㎖) in 3T3-L1 cells. Treatment with Myrrh significantly suppressed the terminal differentiation of 3T3-L1 in a dose-dependent manner, as confirmed by a decrease in triglyceride and Fatty acid and Acetyl-CoA carboxylase. Also, Myrrh exhibited potential adipogenesis inhibition and downregulated the expression of pro-adipogenic transcription factors, such as sterol regulatory element binding protein-1c (SREBP-1c), peroxisome proliferator-activated receptor-γ (PPARγ), CCAAT/enhancer binding proteins α (C/EBPα) and C/EBPβ, and adipocyte expressed genes, such as adipocyte fatty acid binding protein (aP2) and Fas. In addition, lipid accumulation determined by Oil-red O staining showed that Myrrh extract had inhibitory effects on lipid accumulation in 3T3-L1 cells.Conclusions : These results suggest that Myrrh suppresses obesity factors in 3T3-L1 cells. Myrrh may be a useful medical herbs for attenuating metabolic diseases such as obesity.

• Journal of Korean Medical classics
• /
• v.18 no.2 s.29
• /
• pp.70-84
• /
• 2005

장석순(張錫純)$(1860{\sim}1933)$시청말민초시기적의가(是淸末民初時期的醫家), 통과대기소저(通過對其所著)${\ulcorner}$의학충중참서록(醫學衷中參西錄)${\lrcorner}$적제오기제삼권중(的第五期第三卷中), 관우(關于)'논간병치법'(論肝病治法)내용적연구(內容的硏究), 소득출적결론시(所得出的結論是), 기대간병적치법가개괄위평간법(其對肝病的治法可槪括爲平肝法), 산간법(散肝法), 화간법(化肝法), 유간법(柔肝法), 진간법(鎭肝法), 염간법(斂肝法), 보간법(補肝法), 완간법등팔법(緩肝法等八法). 평간법인위(平肝法認爲), 인지원기찰근우신, 맹아우간이유약역손(萌芽于肝而柔弱易損), 고불가관파간기(故不可關破肝氣). 차내경중(且內經中)'궐음불치(厥陰不治), 구지양명(求之陽明)'여금궤중(與金櫃中)'당선실비(當先實脾)', 도이서조간기위관건(都以舒調肝氣爲關鍵), 인차이승강탕여배비서간탕등립방(因此以昇降湯與培脾舒肝湯等立方), 차작위평간약중시료계지(且作爲平肝藥重視了桂枝). 산간법인위(散肝法認爲), 간위목장(肝爲木臟), 구유직향이성향상신전(具有直向而性向上伸展), 소이부응기성(所以符應其性), 이용신미산지(而用辛味散之), 즉가위보법(卽可爲補法). 작위산간약(作爲散肝藥), 기선용인진여맥아(其善用茵蔯與麥芽). 화간법시재간체증대(化肝法是在肝體增大), 혹간중유열(或肝中有熱), 혹간기울체(或肝氣鬱滯), 혹옹창시(或壅脹時), 작위산간달울지법(作爲散肝達鬱之法), 사용료시호(使用了柴胡), 유향(乳香), 몰약(沒藥), 차이활혈약당좌약사용적치법(且以活血藥當佐藥使用的治法), 우기중시료삼칠근(尤其重視了三七根). 유간법시근거(柔肝法是根據)'간악조희윤(肝惡燥喜潤), 조칙간체판경이간화간기즉망동(燥則肝體板硬而肝火肝氣卽妄動), 윤칙간체유화이간화간기장영정(潤則肝體柔和而肝火肝氣長寧靜)'적이론(的理論), 인위재치료중가선용당귀(認爲在治療中可選用當歸), 작약(芍藥), 백자인(柏子仁), 현삼(玄蔘), 구기자(枸杞子), 아교(阿膠), 별갑(鱉甲), 단불가장기사용(但不可長期使用). 기진간법시(其鎭肝法是), 재치료경간(在治療驚癎), 전광(癲狂), 현운(眩暈), 뇌충혈제증시(腦充血諸證時), 작위대표처방선용료진간식풍탕(作爲代表處方選用了鎭肝熄風湯). 염간법시근거기(斂肝法是根據其)'범인원기지탈(凡人元氣之脫), 개탈재간(皆脫在肝)'적독특이론(的獨特理論), 이내복탕등작위대표처방진행치료적방법(以來復湯等作爲代表處方進行治療的方法), 차대제양사용료산수유(且大劑量使用了山茱萸). 보간법시재간기허약이실기조달시사용적치법(補肝法是在肝氣虛弱而失其條達時使用的治法), 인위황기시보간적주약. 완간법시의거(緩肝法是依據)‘간고급(肝苦急), 급식감이완지(急食甘以,緩之)'지설이정적치법(之說而定的治法), 인위단미감초시대표약(認爲單味甘草是代表藥).

• Korean Journal of Pharmacognosy
• /
• v.46 no.1
• /
• pp.12-16
• /
• 2015

Myrrh is a resinous substance obtained from Commiphora trees, which has long been used as an antiseptic agent. A sesquiterpene, $1{\beta}$, $6{\alpha}$-dihydroxyeudesm-4(15)-ene (DE), was isolated from the hot water extract of Myrrh. In the present study, we found that DE attenuates the lipopolysaccharide (LPS)-induced inflammation in BV2 microglial cells. DE significantly inhibited LPS-induced production of pro-inflammatory mediators such as nitric oxide (NO) and prostaglandin $E_2$ ($PGE_2$) in BV2 microglia in a concentration-dependent manner without cytotoxic effect. Furthermore, DE dose-dependently suppressed the protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). These results suggest that DE may be a good candidate to regulate LPS-induced inflammatory response.