• Korean Journal of Veterinary Research
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• v.32 no.1
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• pp.57-61
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• 1992

To determin the accuracy of serological methods in detecting Borna-disease(BD) viral antibodies, 273 experimentally infected rabbit sera were compared by using indirect immunofluorescence antibody test(IFA), serum neutralization test(SN) and enzyme-linked immunosorbent assay(ELISA). One hundred twenty-three serum samples had BD viral antibodies detected by IFA. CELISA antibodies to BD virus were also present in the same one hundred twenty-three serum samples. However, neutralization test antibodies to BD virus were present in 27 of the in rabbit serum samples. Neutralization test was sensitive in comparison with KFA and CELISA. In comparison with IFA, CELISA was both sensitive and specific in detecting BD viral antibodies. These results extend observations made with laboratory animals to the diagnosis of naturally infected animals.

• Parasites, Hosts and Diseases
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• v.21 no.2
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• pp.286-288
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• 1983

폐흡충 감염자에서 나타나는 중추신경계의 병변을 모두 폐흡충이 중추신경계를 침범하여 발생한 것이라고는 할 수 없다. 그러므로 이 경우 원인진단을 위해서는 뇌척수액에 나타나는 폐흡충 특이항체의 측정이 필요하다고 생각된다. 우리는 확인된 뇌폐흡충중 2례와 척수 스파르가눔증 1례, 뇌병변이 없는 폐흡충증 환자 1례와, 기타 중추신경계 질환 환자 10례에서 얻은 뇌척수액을 희석하지 않고 면역효소진단법으로 특이항체를 측정하였다. 그 결과 흡광도 0.25를 양성 기준으로 하면 뇌 폐흡퉁증을 진단할 수 있다고 생각하게 되었다. 폐흡충중 환자 2례의 흉막삼출액에서 특이 IgG 항체가는 혈청에서의 측정치와 다르지 않아, 흉막삼출액도 폐흡충증의 진단에 이용할 수 있다고 생각되었다.

• Korean Journal of Clinical Laboratory Science
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• v.47 no.4
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• pp.168-174
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• 2015

Rheumatoid arthritis (RA) is a chronic inflammatory disease, which is mainly characterized by disease of joints affected with synovial hyperplasia, pathological immune response, and progressive destruction; all of which represent an important social health problem. These provide new insights in its pathogenesis of rheumatoid arthritis diagnosis and disease progression in molecular changes. This review focuses on new serological and immunological markers which seem to be useful in early diagnosis and prognosis of rheumatoid arthritis. Therefore, such tests are widely conducted for serological biomarkers and the developments with such immunological factors to identify patients who are at risk for disease progression. This evidence of the disease based on laboratory medicine could provide the best outcome for patients. Finally, data from recent studies will help to refine the ultimate usefulness of this novel approach for early diagnosis, treatment, and helping clinicians to optimize therapy by using this approach.

• Journal of The Korean Ophthalmological Society
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• v.59 no.11
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• pp.1071-1076
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• 2018

Purpose: To report a case of immunoglobulin G4 (IgG4)-related ophthalmic disease associated with adult xanthogranulomatous disease. Case summary: A 38-year-old male with a history of cholecystectomy visited our clinic for bilateral periorbital swelling. Histopathology of the orbital biopsy showed diffuse infiltration of foamy histiocytes with Touton giant cells and lymphoid follicles, with a diagnosis of adult-onset xanthogranuloma. After excisional biopsy, he was treated with azathioprine and prednisolone. Four years after treatment, he again visited the clinic due to bilateral, yellowish eyelid masses. Serological examinations were all nonspecific findings, except for elevation of IgG and IgG4 levels. Magnetic resonance imaging showed bilateral symmetric soft tissue enlargement with slightly heterogeneous T1/T2 isosignal intensity, with contrast enhancement at the superolateral aspect of extraconal spaces. Excisional biopsy and blepharoplasty were performed. Immunohistochemical sections showed that the IgG4+/IgG plasma cell ratio was 10-20% and the IgG4 plasma cell count was 22/high power field (HPF). His past sections of 2013 from the pathology department were again stained and showed that the IgG4+/IgG plasma cell ratio was 40-50% and the IgG4 plasma cell count was 59/HPF. Thus, he was definitely diagnosed with IgG4-related ophthalmic disease. Conclusions: If there is recurrent eyelid swelling, IgG4-related ophthalmic disease should be considered as a differential diagnosis. And the patient with adult xanthogranulomatous disease can be diagnosed with IgG4-related ophthalmic disease.

• The Korean Journal of Nuclear Medicine
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• v.32 no.4
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• pp.344-353
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• 1998

Purpose: The purpose of this study was to evaluate the diagnostic accuracy of Tc-99m labeled antigranulocyte antibody immunoscintigrapy in the diagnosis of osteomyelitis and compare with the results of triphasic bone scan. Materials and Methods: The study population was 39 patients (22 male, 17 female) who had uncertain diagnoses of osteomyelitis. Fifteen patients had history of orthopedic surgery, and 5 had previous fracture. One milligram of monoclonal antibody against NCA-95 was labeled with 370 MBq of Tc-99m, injected intravenously, and 4 hour images were obtained. Triphasic bone scan images were obtained in 30 patients. The final diagnosis was confirmed by bacteriologic culture, biopsy or long term clinical follow up. Results: Twenty one patients were confirmed to have osteomyelitis (1 acute, 20 chronic). Eighteen patients were without osteomyelitis. Antigranulocyte antibody immunoscintigraphy had a sensitivity of 71% (15/21), and a specificity of 89% (16/18), while the sensitivity and specificity of triphasic bone scan was 93% (13/14) and 38% (6/16), respectively. Antigranulocyte antibody scan showed higher specificity of 100% (11/11) in comparison with 33% (3/9) of triphasic bone scan in patients with history of orthopedic surgery or fracture. Conclusion: Antigranulocyte antibody immunoscintigraphy is more specific than that of triphasic bone scan and may be helpful in patients with history of surgery or fracture. However, sensitivity is lower than triphasic bone scan in the detection of chronic osteomyelitis.

• Pediatric Infection and Vaccine
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• v.28 no.2
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• pp.124-131
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• 2021

Cryptococcus neoformans is a fungus that primarily causes opportunistic infections in immunocompromised hosts. It can also cause various infections in immunocompetent patients. Pulmonary cryptococcosis involving the lymph node is relatively rare in immunocompetent patients. In this report, a previously healthy 17-year-old girl presented with high-grade fever and persistent cough. Chest X-ray and computed tomography (CT) scan revealed an anterior mediastinal conglomerate mass that expanded to the right supraclavicular area. Ultrasound-guided gun biopsy revealed histological evidence of cryptococcosis. Immunological screening tests did not identify immunodeficiency. She recovered completely with a combination therapy of amphotericin B and flucytosine for 2 weeks, followed by fluconazole for 8 months. The characteristics of cryptococcosis involving the mediastinal lymph node and the lung should be understood, and the possibility of cryptococcosis even in immunocompetent hosts should be considered.

• Food Science of Animal Resources
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• v.29 no.4
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• pp.533-538
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• 2009

To suggest an improved diagnostic method for Salmonella spp., analyses were conducted with immunoliposomes and compared with the results from a commercial test kit. One sample out of 36 samples of eggshell was Salmonella-positive via immunoliposomes. In the case of the use of the commercial test kit, six samples out of 36 samples were Salmonellapositive. These Salmonella-positive samples were subjected to biochemical identification tests that confirmed that they were Salmonella-negative. As for the egg content samples, they were Salmonella-negative in both analyses with immunoliposomes and the commercial test kit. The Salmonella analysis with immunoliposomes reduced detection time, by 24 h compared to the commercial test kit. Bacteria, including Acinetobacter baumanni, Chryseomonas luteola, Enterobacter cloacae, Escherichia coli, Escherichia hermannii, Klebsiella pneumonia, Pantoea spp., and Pasteurella pneumotropica, were isolated from the eggshells. Other than Acinetobacter baumanni and Pasteurella pneumotropica most of the isolates were known to frequently appear during egg production processing.

• Journal of fish pathology
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• v.3 no.1
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• pp.11-19
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• 1990

Yellow tail (Seriola quinqueradiata) infected by Pasteurella piscicida have been occurred to mass mortality without showing apparent surface lesions in cage culture farms. In this case, it is necessary to consider countermeasure by rapid diagnosis of infected fish. The purpose of the present study was to investigate usefulness of the direct fluorescent antibody technique(FAT) for rapid diagnosis of pseudotuberclosis of cultured yellowtail caused by P. piscicida. Antibody produced by inoculating rabbit with formalin killed pseudotuberclosis bacteria antigen(strain KNP-2). Immunoglobulin-G(IgG) was purified from antisera by DEAE-cellulose column chromatography and conjugate with fluorescein isothiocyanate. Fluorescein-labeled antisera was purified by sephadex G-25 gel column chromatography. The fluorescein/protein molar ratio of labeled antisera was determined as 8.8-9.5. Diagnosis of cultured yellowtail was examined in cage culture farms which located in Tongyung, kyungnam from July to October 1990. The causative bacteria of pseudotuberclosis could be detected within two hours after the specimens were transferred to the laboratory for FAT, and it showed that FAT could be adapted as a rapid and accurate diagnostic method of pseudotuberclosis in yellowtail.

• Korean Journal of Veterinary Research
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• v.32 no.4
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• pp.569-577
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• 1992

Group A 로타바이러스 VP6에 특이적으로 반응하는 단크론 항체를 이용하여 로타바이러스 감염이 의심되는 돼지 분변으로부터 로타 바이러스를 검색할 수 있는 효소면역측정법을 개발하였다. 이 효소면역측정법에서는 capture antibody로서 protein A-sepharose를 이용하여 단크론 항체로부터 순수 분리한 immunoglobulin을 사용하였으며 detecting antibody는 토끼 면역혈청으로부터 순수 분리한 immunoglobulin에 biotin을 label하여 사용하였다. 개발된 효소면역측정법의 민감도와 특이성을 전자현미경법 및 형광항체법의 것과 비교하여 보았을 때 서로 유사하였으며 분변재료로부터 로타바이러스를 검색하는데 유용한 것으로 나타났다. 개발된 효소면역측정법은 야외로부터 로타바이러스 검색을 위하여 수집된 많은 양의 분변재료를 실험실내에서 screen하는데 유용하게 사용될 것으로 생각된다.

• Journal of Veterinary Clinics
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• v.13 no.1
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• pp.96-101
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• 1996

면역성 용혈성 빈혈증 병견은 임상적으로 허약, 황달, 발열, 침울 및 점막창백을 나타낸다. 본 병의 진단은 구상적혈구증가증, 혈구응집반응 또는 직접적 Coomb시험 양성반응을 확인함과 동시에 용혈성 빈혈의 다른 원인을 배제함으로서 확정하여야 한다. 치료방법에는 적절한 보조적 요법과 함께 면역억압제가 포함된다. 일반적으로 치료에 있어서는 일차적으로 glucocorticoids가 선택되는데 흔히 cytoxan, aziothioprin, vincristine 또는 danazole과 같은 다른 약제와 병용된다. 치료는 그 반응에 근거하여 면역억압제의 용량을 2-4주 간격으로 점차 감량하면서 6개월 또는 그 이상까지 계속한다. 면역억압제에 대한 치료반응은 지연될 수 있고 또 적혈구 보유가 골수로부터 새로운 적혈구를 유리하게될 때까지 병축을 유지시키기에 부적당할 수 있기 때문에 병축의 예후판정은 경계하여야 한다. 심급성 내지 급성 예는 일반적으로 예후가 보다더 좋지 않다.