• Korean Journal of Plant Tissue Culture
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• v.26 no.2
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• pp.137-142
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• 1999

This study was carried out to investigate the effects of bulb size, type of media, depth of planting, and nutrient compositions on sprouting and growth of tissue cultured garlic microbulbs in hydroponic culture. Early and increased sprouting were observed when the microbulbs were transplanted into soil planted in shallow (1 cm in depth), while bulb size and fresh weight of the whole plant increased in deep planting (3 cm in depth). Bulb size have greatly influenced on not only sprouting rate but also plant growth after planting. Large bulbs resulted in high growth rate such as number of leaves, stem width, plant height, and increase in bulb size after planting. It was shown that Oriental nutrient solution (N=0.17, P=0.45, K=1.29, Ca=2.44, and Mg=0.98 me/L) was more effective in sprouting and further growth of microbulbs as compared to Yamazaki solution (N=0.27, P=0.16, K=1.50, Ca=1.36 and Mg=0.78 me/L). Microbulbs grown in mixture of leaf mould + bark + sand or Baroko showed the most vigrous growth.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.60 no.4
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• pp.510-517
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• 2015

Miscanthus has been considered as the most promising bioenergy crop for lignocellulosic biomass production. In Korea, M. sacchariflorus and M. sinensis can be found easily in all regions. It is a great advantage to utilize as important species with respect to genetic and cross-breeding programs materials for creation of novel hybrids. For successful breeding programs, it is important to precisely understand the variability of flowering traits among Miscanthus species as breeding parents materials. In this study, flowering traits were observed daily in 960 germplasms of two Miscanthus species (M. sacchariflorus and M. sinensis) for growing seasons over 2 years. The flowering process was divided into three stages. ST (sprouting time) was recorded when first leaf of the plant emerged on soil. FS1 (flowering stage 1) and FS2 (flowering stage 2) were recorded when flag leaf was firstly observed, and 1 cm of panicle was showing on at least one stem, respectively. For 2013 and 2014, the latest germplasms exerted flag leaf, i.e. September 30 (DOY of FS1 164.1) and September 4 (DOY of FS1 141.0) occurred M. sacchariflorus cv. Geodae 1 and M. sacchariflorus cv. Uram collected from Southern Korea (Jeollanam-do), while Miscanthus germplasms collected from northern Korea (Gyeonggi-do) which emerged the earliest flag leaf in July and August, significantly decreased DOY. For DOY from ST to FS2, M. sacchriflorus germplasms ranged from 140 to 190 days, and 110 to 170 days for 2013 and 2014. The highest frequency showed to 160 days for 2013, and 150 days for 2014. In M. sinensis germplasms, the highest frequency showed to 180 days for 2013, and 170 days for 2014. In the results of correlation between the day of years from ST to FS2 for 2013 and 2014, M. sacchriflorus and M. sinensis showed high coefficient of correlation (0.70 and 0.89). It can be supposed that flowering characteristics of Miscanthus are largely affected by the unique phenotypic characteristic of native habitat than environmental factors of the current planted site. This study for flowering traits of Miscanthus may provides an important information in order to expedite the introduction as breeding materials for creation of new hybrid.

• FLOWER RESEARCH JOURNAL
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• v.18 no.2
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• pp.93-97
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• 2010

The objective of this study was to investigate the effect of hot water and dipping times, and hot- PGR's solution on dormancy breaking of Lilium Oriental Hybrid 'Casa Blanca' and 'Siberia' bulblets regenerated in vitro. Hot-water treatments with distilled water were effective not sprouting rate but shortening of days to sprouting. While $100mg{\cdot}L^{-1}\;GA_{4+7}$ was effective in the dormancy breaking process of bulblets. The content of mitochondrial protein and fumarase activity were highest in bulblet of $100mg{\cdot}L^{-1}\;GA_{4+7}$ addition in hot water treatment. Therefore $GA_{4+7}$ addition in hot-water treatment seem to be the alternative dormancy breaking method to low-temperature treatment in Lilium Oriental Hybrid bulblet regenerated in vitro.

• Korean Journal of Weed Science
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• v.12 no.1
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• pp.1-7
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• 1992

The experiment was conducted to investigate effects of bensulfuron-methyl{methyl 2-((((((4,6-dimethoxypyrimidin-2-yl)amino)carbony)amino)sulfonyl)methyl)benzoate}on bud sprouting, percent regrowth, and regrowth from growth cessation in Eleocharis kuroguwai. Application of bensulfuron-methyl resulted in sprouts of two of three lateral buds in addition to the apical bud of E. kuroguwai. With bensulfuron-methyl the culms elongated from the sprouted buds were killed soon after emergence. However, the buds remained biologically active. During the period of growth cessation the tuber buds respired in a minimum rate, but respiration began to increase with regrowth. At regrowth increase in the respiration was greater in the lateral buds than in the apical bud. Days required to regrowth was 35 days in the suppressed apical bud when applied at the rate of 51 g a. i. ha bensulfuron-methyl, while the suppressed lateral buds sprouted first and second required 29 and 28 dyas, respectively. After regrowth number of new culms was two to three times greater in the lateral buds than in the apical bud.

• FLOWER RESEARCH JOURNAL
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• v.16 no.4
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• pp.260-265
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• 2008

Studies were carried out to elucidate the effect of temperature, glasshouse forcing date and $GA_3$ on the growth and flowering of pot Hydrangea macrophylla Ser. The plant height was elongated in the $5^{\circ}C$ treatments as 38 cm compared with $20^{\circ}C$ treatment as 6.7 cm, and stem length showed the similar results. The leaf length and width was broadened in the lower temperature, and stem diameter showed the same tendency. The first flowering date in the $15^{\circ}C$ and $20^{\circ}C$ treatment shortened markedly than $5^{\circ}C$ treatment. Days to flowering date of $5^{\circ}C$ was 161 day, while it shortened as 88 day in the $15^{\circ}C$ treatment. The flowering rate was 75.8~90.7% in the temperature. The plant height was elongated in the late glasshouse forcing date, and the leaf length and width showed the similar tendency. The plant height increased in the higher concentration of $GA_3$ compared to the control, and leaf length and width showed the similar results. The first flowering day was advanced by 7 days in the $GA_3$ $50mg{\cdot}L^{-1}$ treatment which had been transferred to greenhouse on Dec. 30 compared with the control of which first flowering day was March 17, and the days to first flowering was conspicuously shortened in the late glasshouse forcing treatment. The width of flower cluster was increased in the $GA_3$ at Dec. 30 glasshouse forcing treatment. The flowering rate was markedly decreased as 62.3% in the control of Nov. 15 treatment, but was increased as 97.9% of $GA_3$ $50mg{\cdot}L^{-1}$ of Jan. 15 treatment.

• Horticultural Science & Technology
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• v.29 no.2
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• pp.87-94
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• 2011

This study was carried out to investigate the effects of hot solution soaking and chilling treatment on sprouting and enlargement of bulblets obtained through in vitro culture in Korean native lilies with ornamental values. In vitro bulblets of Lilium cernuum, L. hansonii, L. hansonii for. mutatum, L. leichtlinii, and L. tsingtauense were soaked in distilled water or 100 $mg{\cdot}L^{-1}$ $GA_3$ and $GA_{4+7}$ solution maintained at $35^{\circ}C$ for two hours (hot water treatment) and/or exposed at $4^{\circ}C$ for 0, 4, or 8 weeks (chilling treatment) and then planted in plastic trays filled with media and grown in a greenhouse at $20^{\circ}C$ and under 16 h photoperiod. In all species, no bulblet propagated by tissue culture sprouted without chilling or hot water treatment due to dormancy. For dormancy breaking, $GA_{4+7}$ hot solution treatment increasing sprouting by 55-96%, whereas distilled water or GA was not effective in sprouting. Chilling treatment for 4 weeks induced sprouting by 50-70% in L. cernuum and L. leichtlinii, whereas 8 weeks was needed for sprouting of L. hansonii and L. hansonii for. mutatum. Combined treatment of hot water and chilling treatments synergistically promoted sprouting. Especially, in L. cernuum and L. hansonii, $GA_{4+7}$ hot solution soaking prior to chilling for 4 weeks promoted sprouting by 35-45% compared with the reverse order. Enlargement of bulblets resulted from increase in fresh weight and diameter was promoted by the treatments that increased the sprouting percentage of bulblets. Only in L. cernuum, shoots emerged from bulblets soaked in hot $GA_{4+7}$ solution or chilled at $4^{\circ}C$ and shoot emergence rate was highest in bulblets soaked in hot $GA_{4+7}$ solution and then chilled for 8 weeks. From these results, the most effective method for bulblet sprouting and enlargement was to soak in hot $GA_{4+7}$ solution and then chill for 4 weeks in L. hansonii, hansonii for. mutatum, and leichtlinii, and to soak in hot $GA_{4+7}$ solution and then chill for 4 weeks in L. cernuum and tsingtauense.

• Current Research on Agriculture and Life Sciences
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• v.9
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• pp.147-155
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• 1991

Effects of harvest date, number of the leaves remained on stump after flower cutting, duration of cold treatment on the growth and flowering of L. elegans 'Connecticut King' bulb were tested. Shoot emerging rate increased over 90% by delayed cold treatment, late harvesting time and more leaves remained. Flowering rate increased over 90% when conducted cold treatment of 90 days to the premature bulb at flowering date, the bulb matured for 50 days with 10 leaves and the bulb matured for over 30 days with 20 leaves, and when conducted cold treatment of 50 days to the bulb matured for over 30 days with all leaves. Delayed cold treatment duration shortened days needed to shoot emergence and flowering. Especially days to flower of the premature bulb at flowering date were the shortest of all treatments, and can be flowered within 100 days after planting. In generally, plant height, number of flowers per plant and stem diameter generally increased by longer bulb maturing period after flower cuting, and the premature bulb harvested at flowering date showed poor growing and flowering responses. But, if used the larger size bulb, it is believed that the prematured bulb can be forced to flower normally in current year without bulb maturing period.

• Journal of Bio-Environment Control
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• v.10 no.4
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• pp.251-257
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• 2001

This experiment was conducted to investigate the effect of chitosan on the growth and yield of fall-planted potatoes. The research was carried out in fall-planted potatoes from August 2000 to Aprial 2001 at the height of 250m above sea level in Jeju Island. Soaking for 30 min. of seed potatoes in 200 times diluted solution of 3% liquid chitosan shortened days to emergence by 4 to 6 days. Stem length, number of stems and number of stolons per plant were 56.5 cm, 4.3, and 19.0, respectively, when seed potatoes were soaked for 30 min. in 200 times diluted solution of 3% liquid chitossan. Total potato yield in this treatment reached 2,963 kg per 10a and was significantly greater than in other treatments. Yield of marketable tubers (greater than 30g) per 10a for treatment of seed potatoes soaking in 200 times diluted solution of 3% liquid chitosan and for treatment of foliage spray with 200 times diluted solution of 3% liquid chitosan plus chitosna power (10 kg per 10a) miximg with soil was 2,761 kg and 2,628 kg, respectively. Contents of Mg, Fe and B were the greatest in the treatment of 30 min. soaking of seed potatoes in 200 times diluted solution of 3% liquid chitosanm. The increased contents of these elements are considered to have caused yield increase as a result of increased chlorophyll content for photosynthesis.

• Journal of Bio-Environment Control
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• v.11 no.4
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• pp.193-198
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• 2002

Growth and tuber development of ‘Black Magic’calla lily as affected by the rain shelter with 50％ shading and the tuber size were investigated. Tubers of five grades in size were cultivated in the rain shelter or in the open field (control). Days to emergence of shoots in the rain shelter was accelerated by 4.2 days as compared to the open field. Emergence ratio was higher as the tuber was larger, tubers grown in the rain shelter showed the significance in the growth characteristics as compared to the control. There were no significant differences in the number of flowers per tuber between the rain shelter and the open field. Flower quality was 12.2 cm longer than that in the open field. flowering characteristics was improved with the increasing tuber size. Low infection of soft rot disease of 3∼22％ was found in the rain shelter as compared to the that of 19∼83％ in the open field. Thus tubers produced under the rain shelter showed improved quality, Even if the small tubers with diameters of 0.5∼1.0 cm were cultivated in the rain shelter, the weight and diameters of tuber harvested after approximately 7 months were 50.2 g and 5.7 cm, respectively.

• Current Research on Agriculture and Life Sciences
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• v.4
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• pp.18-21
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• 1986

This study was conducted to clarify the period of induction and awakening of Liatris spicata corm dormancy, and investigated effect of gibberellin treatment on the dormancy break of corm. The results were obtained as follows ; 1. Ratio of sprouting, shooting and rooting were gradually declined after August 30. These ratio showed the lowest to trend on November 14 during experiment and gradually increased after this time. Ratio were excellent as 100% after January 29. 2. Also, average days for sprouting were the longest on the November 14, but it was rapidly declined after January 29. 3. The period of induction and awakening of corm dormancy were late in September and from late January to early February, respectively, in Daegu, Korea. 4. Effects of GA-treatment on the dormancy break of corm were excellent. Especially, its effects on the dormancy break were the deeper in degree of dormancy, the better.