• Korean Journal of Food Science and Technology
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• v.52 no.1
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• pp.40-45
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• 2020

Over 500 genetically modified organisms (GMOs) have been developed since 1996, of which nearly 44% have glufosinate herbicide-tolerant traits. Identification of specific markers that can be used to identify herbicide-tolerant traits is challenging as the DNA sequences of the gene(s) of a trait are highly variable depending on the origin of the gene(s), plant species, and developers. To develop specific PCR marker(s) for the detection of the glufosinate-tolerance trait, DNA sequences of several pat or bar genes were compared and a diverse combination of PCR primer sets were examined using certified reference materials or transgenic plants. Based on both the qualitative and quantitative PCR tests, a primer set specific for pat and non-specific for bar was developed. Additionally, a set of markers that can detect both pat and bar was developed, and the quantitative PCR data indicated that the primer pairs were sensitive enough to detect 0.1% of the mixed seed content rate.

• Korean Journal of Plant Resources
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• v.34 no.5
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• pp.443-450
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• 2021

Peach flesh color is commercially important criteria for classification and has implications for nutritional quality. To breed new yellow-fleshed peach cultivar many cross seedlings and generations should be maintained. Therefore it is necessary to develop early selection molecular markers for screening cross seedlings and germplasm with economically important traits to increase breeding efficiency. For the comparison of transcription profiles in peach varieties with a different flesh color expression, two cDNA libraries were constructed. Differences in gene expression between yellow-fleshed peach cultivar, 'Changhowon Hwangdo' and white-fleshed peach cultivar, 'Mibaekdo' were analyzed by next-generation sequencing (NGS). Expressed sequence tag (EST) of clones from the two varieties was selected for nucleotide sequence determination and homology searches. Putative single nucleotide polymorphisms (SNPs) were screened from peach EST contigs by high resolution melting (HRM) analysis, SNP ID ppa002847m:cds and ppa002540m:cds displayed specific difference between 17 yellow-fleshed and 21 white-fleshed peach varieties. The SNP markers for distinguishing yellow and white fleshed peach varieties by HRM analysis offers the opportunity to use early selection. This SNP markers could be useful for marker assisted breeding and provide a good reference for relevant research on molecular mechanisms of color variation in peach varieties.

• Journal of Plant Biotechnology
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• v.29 no.3
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• pp.151-159
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• 2002

Genetic map and molecular marker have a great importance in improving and facilitating crop breeding program as well as in genome analysis and map-based cloning of genes representing desirable characters. This study aimed at developing RAPD markers and constructing a genetic linkage map using 82 BC$_1$F$_1$individuals originated from the cross between '835' and B$_2$in radish (Raphanus sativus L.). One of the parents for genetic linkage map construction, '835'(P$_1$) of egg type is susceptible to Fusarium wilt and have medium resistance to virus infection and the other parent, B$_2$(P$_2$) of round type, is susceptible to Fusarium wilt and virus, Screening of 394 RAPD primers in BC$_1$F$_1$) population resulted in selecting 128 polymorphic markers which displayed 1:1 segregation pattern. Two markers failed to display 1:1 segregation and showed the segregation ratio skewed to maternal genotype. Selected markers were categorized into 14 linkage group based on LOD score represented by MAPMAKER/EXP program. Five groups composed of single marker among them were excluded from the linkage map, and consequently, the remaining groups are well matched with the number of radish chromosome (n＝9). The linkage map constructed with 128 markers covers 1,688.3 cM and the average distance between markers was 13.8 cM. For developing STS marker, we determined the partial nucleotide sequence of OPE10 marker at both ends and designed a oligonucleotide primer pair based on this sequence. STS PCR using the primer pair displayed a single, clear band of which segregation is perfectly matched with that of OPE10 marker. This implies that RAPD markers could readily convert into clear and reliable STS markers.

• Journal of Korean Society on Water Environment
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• v.23 no.3
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• pp.391-396
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• 2007

Dry washing by air and sand to clean dishes was investigated for establishing a dry washing process in a kitchen. Thirty seasonings and foods as dirt markers and fourteen kinds of dishes were used for the experiment, and washing efficiency was evaluated by COD-MN (mg/L). It was found that air dry washing effectively reduced COD-MN of some dirt markers but not effective at all to some dirt markers. It could be also said that dirt markers would not be completely removed by air dry washing. The shape of dishes also affected COD-MN reduction by air dry washing. Sand dry washing showed excellent efficiency on COD-MN reduction. Combination of air and sand dry washing may be necessary to make a dish dry washing system which is completely independent from water.

• 한국HCI학회:학술대회논문집
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• 2009.02a
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• pp.846-850
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• 2009

본 논문은 픽토그램(Pictogram)의 시각전달 요소를 재해석하고 증강현실 환경에서의 효율적 전달 방법에 대하여 제안한다. 픽토그램은 국경을 넘어 남녀노소 모두가 인지할 수 있어야 함이 규칙이며 불특정 다수라는 사용자들을 위해 정확한 정보를 담고 있는 시각전달 매체로서 공정성, 객관성을 가지고 디자인 되어야 한다. 사용자에게 정보전달 인식을 높이기 위해 표준 픽토그램 과 문화 속에서 발견할 수 있는 행위들을 접목하여 애니메이션으로 제작하고, 이를 다양한 연구분야에서 응용되고 있는 증강현실 (Augmented Reality 또는 AR) 기술에 적용하여 표현하였다. 증강현실은 사용자-콘텐츠 정보전달 매체로서 현실 환경을 기반으로 하는 3 차원공간의 시각정보에 대한 사용자의 인식을 향상시킬 수 있는 방법으로 인간-컴퓨터 상호작용(Human-Computer Interaction)을 제공한다. 본 논문에서 제시된 픽토메이션은 기존 공공장소 어디서나 만날 수 있는 다양한 픽토그램을 패턴화하여 마커로 활용하고 사용자는 현재 널리 보급되고 있는 UMPC 혹은 PDA를 통하여 증강현실 환경에서 제작된 픽토메이션 콘텐츠를 경험해 볼 수 있다. 픽토메이션은 시각커뮤니케이션의 효과를 증가 시킬 수 있고, 정보습득의 주체인 사용자에게 콘텐츠의 종류를 예측할 수 있는 선택의 자유를 준다. 본 논문에서는 멀티미디어디자인 요소를 포함한 콘텐츠와의 실시간 상호작용을 통하여 유희적 요소의 접목을 시도하고 증강현실환경에서 Pictomation을 통하여 사용자들과의 효과적 커뮤니케이션을 위한 방법을 모색하고자 한다.

• The Research Journal of the Costume Culture
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• v.14 no.2
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• pp.286-298
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• 2006

The purpose of this study is to find the proper width of fabric which can bring high efficiency on productivity. We focus on the marking method by comparing and analyzing the marking efficiency of flare skirt. This study employs 4 criteria to mark the flare skirt, which are angle, location, width, and direction, respectively. There can be 3 different angles$(180^{\circ},\;270^{\circ},\;360^{\circ})$, and 2 different locations(the center line and the middle of the pattern) according to fixing of the warp line of the fabric. Also, width can be classified into 2 groups(110cm, 150cm), and marking direction can be grouped into 2(one direction marker and one direction per each size marker). These 4 criteria make $24(3{\times}2{\times}2{\times}2)$ cases for this study. Main findings are follows. Fist, the skirt with the 150cm width has higher efficiency rate than that of 110cm. Second, fixing the warp line at the center line has higher efficiency rate than that in the middle. Finally, per size marker has much higher efficiency than the just direction marker. In sum, we find that 150cm width with the center warp line and per size marker brings the highest efficiency rate.

• The Research Journal of the Costume Culture
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• v.14 no.2
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• pp.310-319
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• 2006

This study intended to compare and analyze marking efficiencies of tailored jacket based on different cloth and production conditions, 'the width of cloth', 'the number of marking pieces' and 'the direction for marking deployment'. The results were as follows. For the two pieces of markers, the efficiency of the cloth width of 110cm was higher than that of 150cm. As the number of markers increased, the efficiency of cloth width of 150cm was higher than that of 110cm. In the case of one-directional deployment, the results obtained was that the efficiency of two markers was higher than that of three markers, while, in the case of bi-directional deployment, the efficiency of three markers was higher than that of two markers. In the case of one-directional deployment for each size, the marking efficiencies were higher in two markers at the cloth width of 110 cm and higher in three markers at the cloth width of 150 cm. Then, in marking efficiencies according to the direction for marking deployment, bi-direction marker was the most efficient marker, followed by one-direction for each size marker and one-direction marker.

• Journal of the Korea Fashion and Costume Design Association
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• v.8 no.1
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• pp.69-79
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• 2006

The purpose of this study is to find the proper width of fabric which can bring high efficiency on productivity. We focus on the marking method by comparing and analyzing the marking efficiency of A-line skirt. This study employs 4 criteria to mark the A-line skirt, which are cutting method, angle, width, and direction, respectively. There can be 2 different cutting methods(fold pattern and add seam pattern at the front and back center line), and 2 different angles(warp angle and bias angle). Also, width of the fabric can be classified into 2 groups(110cm, 150cm), and marking direction can be grouped into 2(one direction marker and one direction per each size marker). These 4 criteria make 16($2^*2^*2^*2$) cases for this study. Main findings are follows. First, the skirt with folded at the center line had higher efficiency rate than the skirt with add seam at the center line. Second, the skirt with the 150cm width has higher efficiency rate than that of 110cm. Third, fixing the warp angle has higher efficiency rate than that of fixing the bias angle at the front and back center line. Finally, one direction per each size marker has much higher efficiency than the one direction marker.

• Magazine of the Korea Institute for Structural Maintenance and Inspection
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• v.18 no.4
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• pp.83-88
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• 2014

구조물에 최적화된 센서 배열(Sensor Array)을 수행하는 것은 센서 네트워크 설계에서 중요한 요소이다. 그러나 센서의 설치와 관리는 구조물이 처해 있는 환경이나, 경제성 그리고 센서의 주파수대역의 제한과 같은 다양한 원인으로 인해 매우 어려울 수 있다. 이 논문에서는 일반적인 문제와 환경에서 현재 사용되고 있는 물리적 센서 대용으로 가상 시각 센서(VVS: Virtual Visual Sensor)를 제안하였다. 가상 시각 센서는 설치가 간편하고 경제적이며 관리가 편하다는 큰 장점을 가지고 있다. 이러한 가상 시각 센서의 기본적인 아이디어는 최첨단 컴퓨터 시각 알고리즘과 마커 추출 기법의 적용으로 이루어진다. 이 연구에서는 가상 시각 센서를 이용하여 모드 형태와 주파수를 추출하는데 용이하다는 점을 보여주며 이를 구조물 건전성 모니터링에 적용할 경우 효율적이라는 점을 입증하였다.

• Proceedings of the Korea Multimedia Society Conference
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• 2001.06a
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• pp.553-557
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• 2001

얼굴 표정을 애니메이션하는 것은 얼굴 구조의 복잡성과 얼굴 표면의 섬세한 움직임으로 인해 컴퓨터 애니메이션 분야에서 가장 어려운 분야로 인식되고 있다. 최근 3D 애니메이션, 영화 특수효과 그리고 게임 제작시 모션 캡처 시스템(Motion Capture System)을 통하여 실제 인간의 동작 및 얼굴 표정을 수치적으로 측정해내어 이를 실제 애니메이션에 직접 사용함으로써 막대한 작업시간 및 인력 그리고 자본을 획기적으로 줄이고 있다. 그러나 기존의 모션 캡처 시스템은 고속 카메라를 이용함으로써 가격이 고가이고 움직임 추적에서도 여러 가지 문제점을 가지고 있다. 본 논문에서는 일반 저가의 카메라와 신경회로망 및 영상처리기법을 이용하여 얼굴 애니메이션용 모션 캡처 시스템에 적응할 수 있는 경제적이고 효율적인 얼굴 움직임 추적기법을 제안한다.