• Title/Summary/Keyword: 마커 효율

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Polymorphism of inter simple sequence repeat markers in Hypsizygus marmoreus (Inter Simple Sequence Repeat(ISSR) 마커를 활용한 느티만가닥버섯(Hypsizigus marmoreus) 종내 다형성 분석)

  • Oh, Youn-Lee;Nam, Yun-Geul;Jang, Kab-Yeul;Kong, Won-Sik;Oh, Min ji;Im, Ji-Hoon;Choi, In-Geol
    • Journal of Mushroom
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    • v.15 no.4
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    • pp.273-278
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    • 2017
  • Hypsizygus marmoreus is a mushroom with abundant flavor and medicinal properties. However, its application is limited by problems such as long cultivation period, low biological efficiency, and microbiological contamination; therefore, there is a substantial need for development of new cultivars of this species. In this study, 55 strains of H. marmoreus were subjected to inter simple sequence repeat (ISSR) analysis to identify markers for the selection of mother strains for breeding from the collected germplasm. ISSR 13 and 15 were confirmed as polymorphic markers. The three strains (KMCC03106, KMCC03107, and KMCC03108) with white cap color were found to be genetically closely related upon UPGMA analysis of both ISSR 13 and 15. Based on the PCR analysis results for ISSR 15, the collected germplasm were differentiated into three groups according to the strain collection year. Thus, ISSR 15 could be a marker for determining the phylogeny of cap color and genetic variations according to the strain collection year. These results suggest that ISSR markers can be effective tools for the selection of mother strains for breeding of H. marmoreus.

Development of Sequence Characterized Amplified Region Markers for Cultivar Identification in Persimmon (감 품종 판별용 SCAR 마커 개발)

  • Cho, Kang Hee;Cho, Kwang-Sik;Han, Jeom Hwa;Kim, Hyun Ran;Shin, Il Sheob;Kim, Se Hee;Chun, Jae An;Hwang, Hae-Sung
    • Horticultural Science & Technology
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    • v.31 no.6
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    • pp.798-806
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    • 2013
  • Precise, fast, and cost-effective identification of crop cultivars is essential for plant breeder's rights. Traditional methods for identification of persimmon cultivars are based on the evaluation of sets of morphological characteristics. However, it is difficult to distinguish closely related cultivars using only morphological traits. This study was conducted to develop DNA markers for identification of the 32 persimmon cultivars in Korea and Japan. A total of 309 randomly amplified polymorphic DNA (RAPD) markers were identified using 40 different random primers. Various number of polymorphic bands ranged from 4 (OPP-08) to 14 (UBC159) were detected with an average of 7.7. Resulting 57 RAPD fragments were selected, and their sequences were determined for developing sequence characterized amplified region (SCAR) markers. As a result, 15 of 57 RAPD fragments were successfully converted to SCAR markers. Single polymorphic bands of the same size as or smaller than the RAPD fragments were amplified depending on SCAR markers. Among these markers, a combination of eight SCAR markers (PS225_200, PSN05_420, PSF13_523, PSN11_540, PS372_567, PS485_569, PSP08_635, and PS631_735) provided sufficient polymorphisms to identify 32 persimmon cultivars. These newly developed markers will be a fast and reliable tool to identify persimmon cultivars.

A Study on Pipe Model Registration for Augmented Reality Based O&M Environment Improving (증강현실 기반의 O&M 환경 개선을 위한 배관 모델 정합에 관한 연구)

  • Lee, Won-Hyuk;Lee, Kyung-Ho;Lee, Jae-Joon;Nam, Byeong-Wook
    • Journal of the Computational Structural Engineering Institute of Korea
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    • v.32 no.3
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    • pp.191-197
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    • 2019
  • As the shipbuilding and offshore plant industries grow larger and more complex, their maintenance and inspection systems become more important. Recently, maintenance and inspection systems based on augmented reality have been attracting much attention for improving worker's understanding of work and efficiency, but it is often difficult to work with because accurate matching between the augmented model and reality information is not. To solve this problem, marker based AR technology is used to attach a specific image to the model. However, the markers get damaged due to the characteristic of the shipbuilding and offshore plant industry, and the camera needs to be able to detect the entire marker clearly, and thus requires sufficient space to exist between the operator. In order to overcome the limitations of the existing AR system, in this study, a markerless AR was adopted to accurately recognize the actual model of the pipe system that occupies the most processes in the shipbuilding and offshore plant industries. The matching methodology. Through this system, it is expected that the twist phenomenon of the augmented model according to the attitude of the real worker and the limited environment can be improved.

A Study on Ontology-based POI Representation for Efficient Marker Search. (AR에서 효율적인 마커 검색을 위한 온톨로지 기반의 POI 표현에 대한 연구)

  • Hwang, Chi-Gon;Lee, Hae-Jun;Yoon, Chang-Pyo
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2017.10a
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    • pp.316-317
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    • 2017
  • Augmented reality is a technology that combines the virtual world based on the real world. This technique requires POI data to recognize the marker and identify the marker. POI data can be easily registered and used by anyone, and there is a need to provide a standard for accessing POI data because a large amount of data is accumulated in the existing augmented reality platform. Therefore, in this paper, we propose a category composition method for POI data integration using ontology which is a paradigm of providing relationship and information sharing. It consists of a combination of the POI core of W3C and OWL, which is the expression language of the ontology.

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Comparative Analysis of Markerless Facial Recognition Technology for 3D Character's Facial Expression Animation -Focusing on the method of Faceware and Faceshift- (3D 캐릭터의 얼굴 표정 애니메이션 마커리스 표정 인식 기술 비교 분석 -페이스웨어와 페이스쉬프트 방식 중심으로-)

  • Kim, Hae-Yoon;Park, Dong-Joo;Lee, Tae-Gu
    • Cartoon and Animation Studies
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    • s.37
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    • pp.221-245
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    • 2014
  • With the success of the world's first 3D computer animated film, "Toy Story" in 1995, industrial development of 3D computer animation gained considerable momentum. Consequently, various 3D animations for TV were produced; in addition, high quality 3D computer animation games became common. To save a large amount of 3D animation production time and cost, technological development has been conducted actively, in accordance with the expansion of industrial demand in this field. Further, compared with the traditional approach of producing animations through hand-drawings, the efficiency of producing 3D computer animations is infinitely greater. In this study, an experiment and a comparative analysis of markerless motion capture systems for facial expression animation has been conducted that aims to improve the efficiency of 3D computer animation production. Faceware system, which is a product of Image Metrics, provides sophisticated production tools despite the complexity of motion capture recognition and application process. Faceshift system, which is a product of same-named Faceshift, though relatively less sophisticated, provides applications for rapid real-time motion recognition. It is hoped that the results of the comparative analysis presented in this paper become baseline data for selecting the appropriate motion capture and key frame animation method for the most efficient production of facial expression animation in accordance with production time and cost, and the degree of sophistication and media in use, when creating animation.

Genetic Diversity of Rice Collections using Subspecies-specific STS Markers (아종특이적 STS 마커를 이용한 벼 품종의 유전다양성 분석)

  • Kim, Bong-Song;Jiang, Wenzhu;Koh, Hee-Jong
    • Korean Journal of Breeding Science
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    • v.41 no.2
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    • pp.101-105
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    • 2009
  • Rice (Oryza sativa L.), the world's most important crop, is usually classified into ssp. indica and japonica based on morpho-physiological traits. In the previous study, we have developed subspecies-specific STS markers (SS markers) to readily discriminate between indica and japonica in O. sativa. In this study, we employed SS markers to investigate the genomic inclination of worldwide collections of O. sativa. A total of 320 varieties were divided into two groups with 63 SS markers. Namely, they formed two distinctive groups, indica and japonica, as expected by their geographic origin. The population structure analysis revealed that the variability of genetic background was greater in indica than in japonica. Some of them, however, exhibited intermediate genomic inclination between indica and japonica. These results are in general agreement with the previous studies, suggesting that SS markers are powerful tools for both determination of subspecies genome and assessment of genetic diversity in rice.

Augmented Reality Interface Using Efficient Hand Gesture Recognition (효율적인 손동작 인식을 이용한 증강현실 인터페이스)

  • Choi, Jun-Yeong;Park, Han-Hoon;Park, Jong-Il
    • 한국HCI학회:학술대회논문집
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    • 2008.02a
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    • pp.91-96
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    • 2008
  • 증강현실(Augmented Reality)을 위한 효과적인 비전 기반 인터페이스 개발은 꾸준히 진행되어 왔으나, 대부분 환경적 제약을 받거나, 특수한 장비 혹은 복잡한 모델을 요구한다. 예를 들어, 마커를 이용하면 구현 상의 편의성과 정확성을 보장하지만, 일반적으로 마커는 환경과 대비되는 모양을 가지기 때문에, 사용자에게 거부감을 줄 수 있으며 무엇보다 복잡한 인터랙션에는 적용되기 힘들다. 한편, 손동작을 이용할 경우, 자연스럽고 다양한 인터랙션을 수행할 수 있지만, 색을 이용한 손동작 인식은 복잡한 환경에서 인식률이 크게 저하되고, 3 차원 모델 기반의 손동작 인식은 많은 연산량을 필요로 한다는 문제점을 가진다. 이로 인해 지금까지 제안된 방법을 증강현실 시스템에 적용하는 데는 한계가 있다. 본 논문에서는 기본적으로 손동작을 이용한 인터페이스를 제안하는데, 손동작 인식을 위한 알고리즘을 효율적으로 개선함으로써, 복잡한 환경에서 적은 연산량으로 자연스러운 인터랙션을 제공하고자 한다. 제안방법은 손목에 컬러 밴드를 착용하고, 색 정보를 이용하여 손을 포함하는 최소 영역을 용이하게 검출함으로써, 손 동작 인식률이 좋아지도록 하였다. 제안된 인터페이스는 손의 자연스러운 움직임을 감지해서 손의 모양과 동작에 따라서 가상의 물체를 자연스럽게 제어할 수 있도록 해 준다. 예를 들어, 손이 지정한 위치에 가상의 물체를 나타내고, 가상의 물체를 잡고 다양한 조작을 하는 등의 제어를 할 수 있다. 다양한 환경에서의 실험 및 사용자 평가를 통해 제안된 인터페이스의 유용성을 검증하였다.

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A Database Creation and Retrival Method of Feature Descriptors for Markerless Tracking (마커리스 트래킹을 위한 특징 서술자의 데이터베이스 생성 및 검색방법)

  • Yun, Yo-Seop;Kim, Tae-Young
    • Journal of Korea Game Society
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    • v.11 no.3
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    • pp.63-72
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    • 2011
  • In this paper, we propose a novel database creation and retrieval method of feature descriptors to support real-time marker-less tracking in the augmented reality environments. Each feature descriptor is encoded by integer and multi-level database is created in order to retrieve a feature descriptor efficiently. The retrieval of a feature descriptor is performed as follows: Firstly, candidate feature descriptors are searched by traversing the multi-level database. Secondly, the euclidean distance between input feature descriptor and each candidate one is compared. The shortest one is retrieved. The proposed method is 16 ms faster than previous KD-Tree method for each feature descriptor.

DNA Fingerprinting Analysis of Natural Monument Gingko Trees Using Microsatellite Markers (Microsatellite 마커를 이용한 은행나무 천연기념물의 DNA 지문 분석)

  • Lee, Jei-Wan;Lee, Min-Woo;Ahn, Ji-Young;Hong, Kyung-Nak;Baek, Seung-Hoon;Kim, Sang-Chul
    • Journal of Korean Society of Forest Science
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    • v.106 no.4
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    • pp.408-416
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    • 2017
  • This study describes DNA fingerprinting analysis of twenty-three natural monument individuals of Ginkgo biloba using eight microsatellite markers. The average number of observed alleles was 6.875, and the expected heterozygosity and the observed heterozygosity were 0.711 and 0.710, respectively. This results were similar to those of the previous studies on Ginkgo trees analyzed by same markers in China and Japan. PIC value and PD were calculated at 0.677 and 0.9999 respectively, indicating a high individual identification efficiency. In fact, all of the natural monument ginkgo trees and additionally analyzed thirteen general ginkgo tress were identified by genotype comparison. PI and PD calculated in three markers (Ging06, Gb60, Gb61) with the highest PIC values calculated in natural monument ginkgo trees were $8.045{\times}10^{-5}$ and 99.99%, respectively. Thus, these three markers could be preferentially used in DNA fingerprinting for identifying ginkgo tree individuals. The results in this study will be useful for management of natural monument ginkgo trees, proliferation of their progeny and genetic identification of individuals selected in breeding process.

A Study on the Development of Field Management System for Underground utility using Self-levelling marker and DGPS. (자동수평마커와 DGPS를 이용한 지하시설물의 현장관리시스템 개발에 관한 연구)

  • Kim, In-Seup
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.27 no.6
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    • pp.733-739
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    • 2009
  • Recently it is being increased rapidly to install magnetic marker and RFID tag on the underground utility lines before backfilling to ensure effective it's management. However, due to changes an attitude and damages of sensors. By pressure and vibration during soil compacting, detecting rate is significantly reduced as well as it allows to use only one line of various pipes since it has an unique frequency. Also it is required too long time to reach to target point with an non-accurate navigational GPS receiver and hard to update existing data base with a manual input of new data in the field. To improve these problems, the researcher developed the field management system that apply with ball typed self-levelling marker which is free from the changes of attitude for sensors during compaction as well as has various radio frequency applicable to many kind of pipes and ensure fast positioning to target point using an incorporated system with PDA based DGPS receiver which allows loading a field GIS software and RFID detector. Further, it provides with viewing all of RFID data on the DGPS receiver screen directly and also input new data to existing data base in the field automatically.