Journal of Korean Society of Forest Science (한국산림과학회지)

Korean Society of Forest Science (한국산림과학회)
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DNA Fingerprinting Analysis of Natural Monument Gingko Trees Using Microsatellite Markers

Microsatellite 마커를 이용한 은행나무 천연기념물의 DNA 지문 분석

  • Lee, Jei-Wan (Division of Forest Genetic Resources, National Institute of Forest Science) ;
  • Lee, Min-Woo (Division of Forest Genetic Resources, National Institute of Forest Science) ;
  • Ahn, Ji-Young (Division of Forest Genetic Resources, National Institute of Forest Science) ;
  • Hong, Kyung-Nak (Division of Forest Genetic Resources, National Institute of Forest Science) ;
  • Baek, Seung-Hoon (Division of Forest Genetic Resources, National Institute of Forest Science) ;
  • Kim, Sang-Chul (Division of Forest Genetic Resources, National Institute of Forest Science)
  • 이제완 (국립산림과학원 산림유전자원과) ;
  • 이민우 (국립산림과학원 산림유전자원과) ;
  • 안지영 (국립산림과학원 산림유전자원과) ;
  • 홍경낙 (국립산림과학원 산림유전자원과) ;
  • 백승훈 (국립산림과학원 산림유전자원과) ;
  • 김상철 (국립산림과학원 산림유전자원과)
  • Received : 2017.09.22
  • Accepted : 2017.12.06
  • Published : 2017.12.31
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Abstract

This study describes DNA fingerprinting analysis of twenty-three natural monument individuals of Ginkgo biloba using eight microsatellite markers. The average number of observed alleles was 6.875, and the expected heterozygosity and the observed heterozygosity were 0.711 and 0.710, respectively. This results were similar to those of the previous studies on Ginkgo trees analyzed by same markers in China and Japan. PIC value and PD were calculated at 0.677 and 0.9999 respectively, indicating a high individual identification efficiency. In fact, all of the natural monument ginkgo trees and additionally analyzed thirteen general ginkgo tress were identified by genotype comparison. PI and PD calculated in three markers (Ging06, Gb60, Gb61) with the highest PIC values calculated in natural monument ginkgo trees were $8.045{\times}10^{-5}$ and 99.99%, respectively. Thus, these three markers could be preferentially used in DNA fingerprinting for identifying ginkgo tree individuals. The results in this study will be useful for management of natural monument ginkgo trees, proliferation of their progeny and genetic identification of individuals selected in breeding process.

본 연구는 국내 천연기념물 은행나무 23개체를 대상으로 8개의 microsatellite 마커를 이용하여 DNA 지문분석을 수행하였다. 그 결과, 평균 대립유전자수는 6.875개, 평균 이형접합도 관찰치와 기대치는 각각 0.711과 0.710로 나타났다. 이러한 수치는 동일한 마커로 일본 및 중국 은행나무에서 분석된 기존 연구 결과와 유사하였다. 분석된 8개 마커의 다형성 정보량(PIC) 값 및 개체식별력(PD)은 각각 0.677과 0.9999로 높은 개체식별 효율을 나타내었다. 실제 유전형을 비교한 결과에서도 모든 천연기념물 은행나무와 추가로 분석된 일반 은행나무 13개체가 모두 식별되었다. 천연기념물 은행나무에서 계산된 PIC 값을 기준으로 상위 3개의 마커(Ging06, Gb60, Gb61)에서 계산된 개체인식력과 개체식별력이 각각 $8.045{\times}10^{-5}$ 및 99.99%로 계산되므로, 이들 3개의 마커가 은행나무 개체식별을 위한 DNA 지문 분석에 우선 적용이 가능할 것이다. 본 연구의 DNA 지문 분석 결과는 천연기념물 은행나무의 관리와 후계목 육성 및 은행나무 선발 개체의 유전적 동일성 검정에 유용한 자료로 활용될 것으로 판단된다.

Keywords

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