• Journal of the Korean Home Economics Association
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• v.48 no.2
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• pp.85-94
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• 2010

Comparative analysis of marker efficiency in blouse patterns, based on different sleeve designs, was carried out. Sleeve designs used included set-in-sleeve, laglan sleeve, and epaulet sleeve. The two types of epaulet sleeves, A and B, are based on pattern arrangement methods of center back. Cloth and production conditions are the width of cloth, the number of marking pieces, and the direction for marking deployment. A blouse pattern saved to the PAD CAD System was graded with different sizes and arranged for industrial purpose to calculate the marker efficiency in different conditions. The results were as follows. On the whole, the marker efficiency of small pattern sized set-insleeve was higher than laglan and epaulet sleeve designs. It was also established that marker efficiency is dependent on cloth and production conditions. For small number of marking pieces, efficiency was higher in the condition of 110cm cloth widths compared with that condition of 150cm cloth widths. However the efficiency of large number of marking pieces was higher in the condition of 150cm cloth widths.

• Journal of Mushroom
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• v.15 no.3
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• pp.139-144
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• 2017

A. bisporus is the fifth most cultivated mushroom in Korea, and approximately 10,757 tons were cultivated in 2015. The genetic diversity of collected strains in Korea and commercial cultivars was analyzed using inter-simple sequence repeat (ISSR) markers. ISSR markers known to be comparable among A. bisporus spp. were selected from various markers. Totally, 16 markers, namely the ISSR markers 807, 808, 810, 811, 834, 835, 836, 841, 842, P3, P8, P17, P22, P30, P38, and P39, were evaluated to discriminate between ASI 1110, 1114, 1115, 1238, 1246, 1365, 1366, and 1369 for selecting suitable markers in 16 markers. The ISSR markers P31, P38 and P39 exhibited various fingerprints that could help classify the strains in species. Using the three markers, genetic relationships among 39 strains, including commercial cultivars, such as SaeA and SaeYeon, were analyzed using the UPGMA method. The results of the analysis of the genetic relationships between commercial cultivars and collected strains in Korea confirmed that the commercial cultivars were different from the collected strains in Korea. These results suggested that the ISSR markers P31, P38, and P30 could be used for selecting the commercial cultivars of A. bisporus.

• Horticultural Science & Technology
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• v.33 no.5
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• pp.722-729
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• 2015

Powdery mildew (PM) caused by Podosphaera aphanis is a major disease that can result in significant yield losses in strawberry (Fragaria ${\times}$ ananassa Duchesne). For preventing PM, pesticides are usually applied in strawberry. In this study, molecular markers were developed to increase breeding efficiency of PM-resistance cultivars by marker-assisted selection (MAS). An $F_2$ population derived from a cross between PM-resistance 'Seolhyang' and PM-susceptibility 'Akihime' was evaluated for disease resistance to PM and RAPD (random amplification of polymorphic DNA)-BSA (bulked segregant analysis). Among 200 RAPD primers tested, OPE10 primer amplified a 311bp-band present in with 331bp. Sequence alignment performed for searching polymorphisms and six single nucleotide polymorphism (SNP) were found in amplified regions. To develop polymorphic marker for distinguishing between resistant and susceptible, RAPD was converted to cleaved amplified polymorphic sequence (CAPS) marker. Among restriction enzymes associated with six SNPs, Eae I (Y/GGCCR) was successfully digested to 231bp in susceptible. The results suggest that the selected CAPS marker could be used for increasing efficiency of selecting powdery mildew resistant strawberry in breeding system.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.13 no.11
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• pp.2335-2340
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• 2009

This paper presents how to implement Markerless Augmented Reality and how to create and apply reference data sets. There are three parts related with implementation: setting camera, creation of reference data set, and tracking. To create effective reference data sets, we need a 3D model such as CAD model. It is also required to create reference data sets from various viewpoints. We extract the feature points from the mode1 image and then extract 3D positions corresponding to the feature points using ray tracking. These 2D/3D correspondence point sets constitute a reference data set of the model. Reference data sets are constructed for various viewpoints of the model. Fast tracking can be done using a reference data set the most frequently matched with feature points of the present frame and model data near the reference data set.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2009.10a
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• pp.204-207
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• 2009

This paper presents how to implement Markerless Augmented Reality and how to create and apply reference data sets. There are three parts related with implementation: setting camera, creation of reference data set, and tracking. To create effective reference data sets, we need a 3D model such as CAD model. It is also required to create reference data sets from various viewpoints. We extract the feature points from the model image and then extract 3D positions corresponding to the feature points using ray tracking. These 2D/3D correspondence point sets constitute a reference data set of the model. Reference data sets are constructed for various viewpoints of the model. Fast tracking can be done using a reference data set the most frequently matched with feature points of the present frame and model data near the reference data set.

• Journal of Plant Biotechnology
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• v.44 no.3
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• pp.243-263
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• 2017

This study is to establish the varietal discrimination based on DNA profiling of different varieties of rice. We examined the genetic distance among Korean rice varieties using allele frequencies and a genetic diversity analysis with Simple Sequence Repeats (SSRs) markers. The analysis of the genetic diversity and genetic relationships of 243 Korean rice varieties was varied out using 20 SSRs markers. A total of 268 alleles were detected, ranging from 6 to 32, with an average of 13.45 alleles per locus, and and average of gene diversity (GD) of 0.556. Seven SSR markers were selected as key markers for discrimination among the Korean rice varieties. Concerning the results, 243 varieties (100%) were discriminated among by using acrylamide gel and fragment analyzer-based markers. In conclusion, this study provides useful basic data that can be utilized concerning Korean rice varieties breeding and development. In addition, we will have to manage and conserve as a valuable genetic resource, without losing the diversity of Korean rice varieties.

• Proceedings of the Korean Information Science Society Conference
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• 1998.10c
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• pp.668-670
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• 1998

테크놀로지 매핑의 효율성은 매핑된 회로의 지연시간과 회로의 면적에 의해서 평가되어진다. 특히 순차회로에서는 레지스터 사이의 조합회로의 최대지연시간에 의해서 전체회로의 지연시간이 결정된다. 본 논문에서는 순차회로에 대한, 건설적인(Constructive) 단계와 반복적인(Iterative) 단계의 리타이밍 기술과 퍼지 논리에 의해 향상된 FPGA 매핑 알고리즘을 소개한다. 주어진 초기회로는 건설적인 방법에 의하여 FPGA회로로 초기매핑되어진후 반복적인 리타이밍에 의하여 매핑회로의 효율을 높이게된다. 초기회로에 주어진 여러 가지 기준들은 결정 함수(Decision Making)에 대한 퍼지 이론 법칙의 계층적인 구조에 의해 연결되어져 있다. 제안된 매퍼는 MCNC 밴치마커의 실험을 통해 지연시각과 면적에서 기존 매핑시스템의 성능을 능가함을 보여준다.

• Journal of Life Science
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• v.30 no.3
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• pp.239-249
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• 2020

Persimmon flowers are fruit-bearing female, pollen-bearing male, or hermaphrodite, containing both a pistil and a stamen. Using prominent PCNA persimmons as male parents is very important for breeding programs, as the selection procedure for new cultivars bearing male flowers requires a long time and a large field in a traditional crossbreeding method. To improve breeding efficiency through early selection of male flower-bearing plants at the seedling stage, analysis was performed on 88 major cultivars whose gender expressions are known, using two male flower selection markers recommended by Akagi et al. The OGI locus marker and DISx-AF4S marker results showed that 83 and 72 cultivars, respectively, matched in terms of gender expression and marker analysis. For the OGI locus marker, 890 plants were selected from 2,509 seedlings obtained from crossbreeding with the mother plant "Migamjosang," which was the breeding cultivar. Comparing the gender expression of the flowers and the marker with 1,186 crossbred seedlings, excluding the unfertilized and dead plants, inconsistencies were found in 401 plants (33.8%). For the DISx-AF4S marker, 636 plants were selected from 889 seedlings obtained from 12 cross-combinations. The results of the sex expression and marker analysis were compared to 379 plants, excluding the unfertilized and dead plants, and inconsistencies were found in 247 plants (65.2%). These results indicate that the examined DISx-AF4S and OGI locus markers would not be suitable for utilization in the breeding field.

• Korean Journal of Plant Resources
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• v.26 no.4
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• pp.491-502
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• 2013

Persimmon (Diospyros kaki Thunb) fruit is one of the most important fruit and have been cultivated from ancient times in Korea. In this study, we found 16 EST-SSR markers that contained one or more EST-SSR sites from 246 cDNA sequences. The developing of EST-SSR marker analysis from 42 persimmon cultivars was compared by genetic relationships and morphological relationships using 6 qualitative traits (fruit related 6 traits) and 19 quantitative traits (flower related 19 traits). In this study, 25 primer sets were tested to identify PCR polymorphism and 14 potential EST-SSR primer pairs were selected. The result of morphological relationship EST-SSR marker analysis showed that the coefficient 0.02 was difficult to categorize in several groups. And then, coefficient 0.77 of genetic relationship showed that the group was classified as four groups. The result of correlation distance between genetic relationship and morphological relationship were investigated was low significance (-0.03). Our results also provided an optimized method for improvement of breeding efficiency and introduce of superior character at persimmon cultivars using EST-SSR markers which was useful for further investigation.

• Journal of Korean Society of Forest Science
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• v.105 no.4
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• pp.422-428
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• 2016

Ginkgo (Ginkgo biloba L.) is one of the most appropriate roadside trees because of a good transplantation nature and ability to grow well in urban environment. Ginkgo is a dioecious species. Sex discrimination of ginkgo is possible through comparing morphological characters of reproductive organs. However, it needs more than about twenty years for reproductive organs to appear after sexual maturity. Until now, ginkgo trees for roadside plantation have been planted without discriminating the sex because ginkgo trees have been usually planted before sexual maturity. Ginkgo nuts from the female ginkgo trees planted along the roadside emit a foul odor, and make much pollution on the streets. Thus in this study a novel SCAR marker (SCAR-GBM) for the early sex discrimination was developed. Primers were developed on the basis of the sequence of male-specific RAPD variants reported previously. False-negative problem of SCAR marker, probably caused by dominant nature, was resolved by using multiplex PCR using primers of both the SCAR-GBM and a universal primer set of atp1 region in mitochondria DNA, which resulted in improved discrimination efficiency. The results showed that DNA bands of 1,039 bp were commonly amplified by the atp1 primer set in male and female trees, and SCAR-GBM markers of 675 bp were specifically amplified only in male trees. Reproducible and specific discrimination of the multiplex PCR was finally confirmed by applying multiple male and female individuals.