• Polymer Science and Technology
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• v.24 no.3
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• pp.254-260
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• 2013

• Horticultural Science & Technology
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• v.31 no.6
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• pp.772-781
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• 2013

The objective of this study was to develop simple sequence repeat (SSR) markers from expressed sequence tags (EST) of lettuce (Lactuca sativa) and identify 9 germplasms from 3 wild species of lettuce and 61 commercial cultivars using the developed EST-SSR markers. A total of 81,330 lettuce ESTs from NCBI databases were used to search for SSR and 4,229 SSR loci were identified. The highest proportion (59.12%, 2500) was represented by trinucleotide, followed by dinucleotide (29.70%, 1256) and hexanucleotide (6.62%, 280) among SSR repeat motifs. Totally 474 EST-SSR primers were developed from EST and a random set of 267 primers was used to assess the genetic diversity among 9 germplasms and 61 cultivars. Out of 267 primers, 47 EST-SSR markers showed polymorphism between 7 cultivars. Twenty-six EST-SSR markers among 47 EST-SSR markers showed high polymorphism, reproducibility, and band clearance. The relationship between 26 markers genotypes and 70 accessions was analyzed. Totally 127 polymorphic amplified fragments were obtained by 26 EST-SSR markers and two to nine SSR alleles were detected for each locus with an average of 4.88 alleles per locus. Average polymorphism information content was 0.542, ranging from 0.269 to 0.768. Genetic distance of clusters ranged from 0.05 to 0.94 between 70 accessions and dendrogram at a similarity of 0.34 gave 7 main clusters. Analysis of genetic diversity revealed by these 26 EST-SSR markers showed that the 9 germplasms and 61 commercial cultivars were discriminated by marker genotypes. These newly developed EST-SSR markers will be useful for cultivar identification and distinctness, uniformity and stability test of lettuce.

• Proceedings of the Korea Information Processing Society Conference
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• 2008.11a
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• pp.1529-1532
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• 2008

이동 에이전트는 플랫폼 간을 이주하며 자기 복제를 통한 작업 분배가 가능하다. 이러한 점에 의해 최근 이동 에이전트는 분산 처리 기반 기술로 각광 받고 있다. 그러나 이러한 에이전트의 이주, 복제능력은 악의적인 플랫폼과 이동 에이전트의 공격에 대한 약점이 되고 있다. 그 중 재실행 공격은 에이전트의 반복 수행을 통해 에이전트를 공격하는 기법이다. 이에 대한 방지 기법으로 트립 마커를 이용하여 동적인 수행 결정이 가능한 연구가 있다. 그러나 이 기법은 이동 에이전트가 위치한 플랫폼에 따라 이동 에이전트의 복제가 제한 받는다는 단점이 있다. 본 논문에서는 이동 에이전트의 복제가 가능한 외적 재실행 방지 기법을 제안한다. 본 기법은 트립 마커 생성을 담당하는 트립 마커 서버를 두어 플랫폼에 상관없이 유연한 이동 에이전트의 복제가 가능하다. 또한 비대칭키 기법을 이용한 비밀 통신을 통해 재실행 공격으로부터 이동 에이전트를 방어한다.

• Journal of Mushroom
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• v.19 no.1
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• pp.76-80
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• 2021

To develop a method for the differentiation of Pleurotus ostratus cultivars, the multiplex-simple sequence repeat (SSR) primer set based on the SSRs obtained from whole genomic DNA sequence analysis was designed with two polymerase chain reaction (PCR) primer sets. These SSR primer sets were employed to distinguish 10 cultivars and strains. Twenty polymorphic markers were selected based on the genotyping results. PCR with each primer produced 1-4 distinct bands ranging in size from 150 to 350 bp, which was within the expected range. However, since a sole SSR marker was unable to detect polymorphisms in every cultivar, multiplex PCRs with composite PCR primer sets were employed. The multiplex primer, "166+115," completely discriminated 12 cultivars and strains with 40 loci, which were 12 more than the simple arithmetic addition of each locus of the primers 115 and 166. These results might be useful to provide an efficient method for the differentiation of P. ostreatus cultivars with separate PCRs for the quality control of spawn and protection of breeders' rights.

• The Journal of the Korea Contents Association
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• v.12 no.6
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• pp.9-20
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• 2012

In computer vision, it is very important to obtain reliable corresponding feature points. However, we know it is not easy to find the corresponding feature points exactly considering by scaling, lighting, viewpoints, etc. Lots of SIFT methods applies the invariant to image scale and rotation and change in illumination, which is due to the feature vector extracted from corners or edges of object. However, SIFT could not find feature points, if edges do not exist in the area when we extract feature points along edges. In this paper, we present a new placement method of marker to improve the performance of SIFT feature detection and matching between different view of an object or scene. The shape of the markers used in the proposed method is formed in a semicircle to detect dominant direction vector by SIFT algorithm depending on direction placement of marker. We applied De Bruijn sequence for the markers direction placement to improve the matching performance. The experimental results show that the proposed method is more accurate and effective comparing to the current method.

• Journal of the Korea Society of Computer and Information
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• v.13 no.5
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• pp.187-193
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• 2008

Interface technologies for a user to control home automation system in wearable computing environment has been studied recently. This paper proposes a new interface method for a disabled person to control home automation system in wearable computing environment by using EOG sensing circuit and marker recognition. In the proposed interface method, the operations of a home network device are represented with human readable markers and displayed around the device. A user wearing a HMD, a video camera, and a computer selects the desired operation by seeing the markers and selecting one of them with eye movement from the HMD display The requested operation is executed by sending the control command for the selected marker to the home network control device. By using the EOG sensing circuit and the marker recognition system a user having problem with moving hands and fit can manipulate a home automation system with only eye movement.

• Korean Journal of Poultry Science
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• v.47 no.4
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• pp.219-227
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• 2020

As the earth's average temperature rises, crop and livestock productions are at risk. Chickens are sensitive to heat stress, and increased temperatures may have adverse effects on their production performance and animal welfare. Reliable stress measurements are crucial for heat stress adaptation. Therefore, various measurement methods and biomarkers are used to evaluate poultry stress levels. Heat shock proteins (HSPs) are heat sensitive biological markers that are highly expressed under stress, thereby acting as a cellular thermometer. HSPs also have chaperone activity, which protects cells from heat stress. This review details the role of HSP70 as a molecular chaperone and biomarker for heat stress, which is important for breeding climate-adaptable, thermo-tolerant poultry.

• Horticultural Science & Technology
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• v.35 no.1
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• pp.98-107
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• 2017

A DNA profile database was constructed to investigate the genetic relatedness of 72 germplasm samples of Pyrus and related cultivars using microsatellite markers. Three P. pyrifolia, four P. commus, and one P. betulifolia cultivars with different morphological traits were screened using 387 pairs of microsatellite primers. A core set of 11 primer pairs was selected to obtain 133 polymorphic amplified fragments meeting three criteria: high polymorphism information contents (PIC), high repeatability, and distinct allele patterns. The number of alleles per locus ranged between 4 and 22. Average PIC was 0.743 (range: 0.557 - 0.879). Cluster analysis using the unweighted pair - group method with arithmetical average (UPGMA) separated the 72 pear cultivars and germplasm samples into four major groups: Chinese, European pears, and a cluster of 55 Asian pears that could be reclassify into two subcluster, I - $1^{st}$ and II - $2^{nd}$, according to pedigree information. Almost all of the cultivars were discriminated by 11 microsatellite marker genotypes. The microsatellite DNA profile database may be utilized as tool to verify distinctness, uniformity, and stability between candidate cultivar, and to verify in the distinctness of existing cultivars.

• Horticultural Science & Technology
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• v.33 no.5
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• pp.722-729
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• 2015

Powdery mildew (PM) caused by Podosphaera aphanis is a major disease that can result in significant yield losses in strawberry (Fragaria ${\times}$ ananassa Duchesne). For preventing PM, pesticides are usually applied in strawberry. In this study, molecular markers were developed to increase breeding efficiency of PM-resistance cultivars by marker-assisted selection (MAS). An $F_2$ population derived from a cross between PM-resistance 'Seolhyang' and PM-susceptibility 'Akihime' was evaluated for disease resistance to PM and RAPD (random amplification of polymorphic DNA)-BSA (bulked segregant analysis). Among 200 RAPD primers tested, OPE10 primer amplified a 311bp-band present in with 331bp. Sequence alignment performed for searching polymorphisms and six single nucleotide polymorphism (SNP) were found in amplified regions. To develop polymorphic marker for distinguishing between resistant and susceptible, RAPD was converted to cleaved amplified polymorphic sequence (CAPS) marker. Among restriction enzymes associated with six SNPs, Eae I (Y/GGCCR) was successfully digested to 231bp in susceptible. The results suggest that the selected CAPS marker could be used for increasing efficiency of selecting powdery mildew resistant strawberry in breeding system.

• Journal of Life Science
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• v.34 no.6
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• pp.434-441
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• 2024

Mammary gland tumors are the most common tumors detected in non-spayed female dogs and pose a significant clinical challenge. Due to the strong similarity between canine mammary tumors (CMT) and human breast cancer (HBC), biomarkers identified in HBC can also be detected in CMT. These biomarkers have been shown to offer valuable insights into early diagnosis, prognosis, and treatment strategies. The purpose of this article is to provide a concise overview of CMT biomarkers based on the current literature. Traditional treatments for CMT in dogs typically begin with surgery, followed by chemotherapy, radiotherapy, or hormonal therapy. However, these treatments alone are not always fully effective. A diagnostic biomarker can detect the presence of a disease or the characteristics of a disease and classify an individual's status. Prognostic biomarkers focus on predicting the expected progression, recurrence, or survival of the disease in patients. By utilizing advances in understanding the mechanism of canine-specific mammary gland tumors, the estimation of biomarkers offers hope for improved outcomes in cancer patients. Novel technologies, such as single-cell RNA sequencing analysis, could provide a valuable resource for deciphering intra- and inter-tumoral heterogeneity. This review paper explores current research on CMT biomarkers and suggests directions for their development.