• Title/Summary/Keyword: 라이

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Isolation of an Invertebrate-type Lysozyme from the Body Wall of Spoon Worm, Urechis unicinctus (개불의 체벽으로부터 i-type 라이소자임의 정제)

  • Oh, Hye Young;Park, Nam Gyu
    • Journal of Life Science
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    • v.28 no.3
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    • pp.300-306
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    • 2018
  • Lysozymes are innate immune factors that play a critical role in the defense against pathogens in various invertebrate animals including spoon worms. In this study, an invertebrate-type lysozyme was isolated from the body wall of spoon worm, Urechis unicinctus. The acidified body wall extract was partially separated using a Sep-Pak C18 cartridge. Among the fractions, the materials that were eluted with 60% methanol/0.1% trifluoroacetic acid showed the most potent antimicrobial activity against Bacillus subtilis KCTC 1021. A series of high performance liquid chromatography (HPLC) steps were then utilized to isolate a single antimicrobial absorbance peak. The molecular weight of the antimicrobial peak was approximated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), which was approximately 13 to 14 kDa. The partial primary structure of this antimicrobial protein that was analyzed, using LC-MS/MS, was CTGGRPPTCEDYAK (1611.69 Da). Homology search of these fourteen residues, using the National Center for Biotechnology Information Basic Local Alignment Search Tool (NCBI BLAST), revealed that the isolated protein was similar to the invertebrate-type lysozymes described in other animals. Then, the antimicrobial and lysozyme enzymatic (muramidase) activities of this protein were assessed. The isolated protein possessed antimicrobial activity and potent muramidase activity, which were comparable to those of hen egg white lysozyme. Therefore, the isolated protein was designated as Urechis unicinctus invertebrate-type lysozyme from the body wall, Uu-iLysb.

Effect of Herbicide 'Kerb' on Growth of Perennial Ryegrass (Lolium perenne L. 'Accent') and Zoysiagrass (Zoysia japonca 'Zenith') under Shading Treatments (차광조절하에서 제초제 Kerb가 퍼레니얼 라이그래스와 한국잔디의 생육에 미치는 영향)

  • Ham, Tae-Hwan;Yun, Jong-Jin;Lee, Sang-Gi;Ku, Ja-Hyung
    • Asian Journal of Turfgrass Science
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    • v.25 no.1
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    • pp.52-58
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    • 2011
  • In order to investigate an artificially transition method of perennial ryegrass(Lolium perenne L. 'Accent') overseeded on zoysiagrass (Zoysia japonca Zenith') with Kerb (3,5-jichloro-N (1,1-dimethyl-2-propynyl). We observed turfgrass growth and physiological responses of the two turfgrasses under the shading treatments. The experiment was conducted in greenhouse during April- August. Shading was generally using a black shade net product whose the shade level was done into 0, 35 and 75%, actual light intensities were 589, 378 and $135\;{\mu}mol{\cdot}m^{-2}{\cdot}s$ PAR, respectively. The four levels of Kerb treatment were 0, 0.1 0.2, and $0.4\;g{\cdot}m^{-2}$. As shading was getting increased, growth of perennial ryegrass was increased slowly, but zoysiagrass was reduced and almost all of experiments showed significant differences statistically. Perennial ryegrass exhibited inclination that growth was reduced in more than Kerb $0.4\;g{\cdot}m^{-2}$ by shades. On the other hand, it was shown that the growth of zoysigrass was reduced by shades largely and it was not effected by Kerb. Through this study, it was determined that Kerb is effective for elimination of overseeded perennial ryegrass on zoysiagrass. Moreover, when excluding perennial ryegrass under the condition like shade, it is desirable for treating high concentration more than the basic processing amount.

Seasonal Differences in Turf Quality of Kentucky Bluegrass, Perennial Ryegrass, Tall Fescue and Mixtures Grown under a Pure Sand of USGA System (USGA 모래 지반구조에서 캔터키블루그래스, 퍼레니얼 라이그래스, 톨훼스큐 및 혼합구 잔디의 연중 품질 차이)

  • Kim, Kyoung-Nam;Nam, Sang-Yong
    • Asian Journal of Turfgrass Science
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    • v.19 no.2
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    • pp.151-160
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    • 2005
  • Research was initiated to investigate seasonal turf quality under a sand-based USGA soil system. USGA system 45 centimeters deep was built with rootzone layer, intermediate layer, and drainage layer. Six turfgrass entries were comprised of 3 blends and 3 mixtures from cool-season grasses (CSG). Turfgrass quality ratings were best in spring and fall, especially early May to early July and late August to early November. Kentucky bluegrass(KB) consistently produced the greatest quality, while perennial ryegrass (PR) the poorest. Intermediate turf quality between KB and PR was observed with tall fescue (TF). Among CSG mixtures it increased with KB but decreased with PR. There were considerable variations in summer turf performance. No summer drought injury was found in KB and TF. However, PR showed poor performance through summer as compared with other CSG. Results demonstrate that KB was the best and PR the worst among CSG grown in a sand-based USCA soil system.

A Hybrid Index Allocation Scheme Considering both Energy Efficiency and Data Access Frequencies in Mobile Broadcast Environments (브로드캐스트환경에서 에너지효율과 데이터접근빈도를 동시에 고려한 하이브리드 인덱스배 치기법)

  • Park JieHyun;Park KwangJin;Kang Sang-Won;Kim Jongwan;Im SeokJin;Hwang Chong-Sun
    • Proceedings of the Korean Information Science Society Conference
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    • 2005.11b
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    • pp.46-48
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    • 2005
  • 이동 컴퓨팅 환경에서 다수의 이동 클라이언트들에게 데이터를 전달할 때는 클라이언트들의 동시 데이터 접근을 지원하는 브로드캐스트 기법을 사용하면 무선 채널 대역폭의 협소함과 클라이언트 측의 에너지 제약과 같은 단점을 해결할 수 있다. 기존 기법들은 클라이언트의 데이터에 대한 접근빈도(access frequencies)와 클라이언트의 에너지 효율(energy efficiency)을 동시에 고려하지 않았다. 따라서 원하는 데이터가 올 때까지 계속해서 채널을 들어야 함으로 인해 에너지 소비를 많이 하거나, 데이터를 얻을 때까지 추가한 많은 양의 정보에 따른 지연이 발생하는 단점이 있다. 본 논문에서는 클라이언트의 에너지 절약을 위한 tuning time을 최소화하고 실제 데이터를 얻을 때까지 소요되는 access time의 효율을 높이기 위해 데이터의 접근빈도와 에너지 효율을 동시에 고려하는 HIDAF: Hybrid Index considering Data Access Frequencies 기법을 제한한다. 제안하는 기법은 트리기반 기법과 해싱기반 기법을 함께 적용하여 구성한 인덱스를 브로드캐스트 주기에 배치한다. HIDAF 기법은 데이터 접근빈도를 고려한 트리기반 인덱스를 배치함으로써 데이터를 얻기 위한 클라이언트들의 평균 access time을 줄일 수 있고, 이러한 인덱스에 해싱기반 기법을 추가함으로써 클라이언트의 에너지 효율을 최소화하는 새로운 브로드캐스팅 기법이다. HIDAF 기법은 브로드캐스트 추기에 데이터의 접근빈도를 고려한 인덱스를 교차로 추가하여 핫 데이터에 대한 클라이언트들의 access time을 줄임으로써 전체 사용자에 대한 평균 access time을 최소화하는 동시에 클라이언트들의 제한된 에너지 소비를 최소화하는데 목적이 있다. 제안기법에 대한 평가는 수학적 분석을 통해 HIDAF 기법과 기존의 브로드캐스트 기법의 성능을 비교 분석한다.하였으나 사료효율은 증진시켰으며, 후자(사양, 사료)와의 상호작용은 나타나지 않았다. 이상의 결과는 거세비육돈에서 1) androgen과 estrogen은 공히 자발적인 사료섭취와 등지방 침적을 억제하고 IGF-I 분비를 증가시키며, 2) 성선스테로이드호르몬의 이 같은 성장에 미치는 효과의 일부는 IGF-I을 통해 매개될 수도 있을을 시사한다. 약 $70 {\~} 90\%$의 phenoxyethanol이 유상에 존재하였다. 또한, 미생물에 대한 항균력도 phenoxyethanol이 수상에 많이 존재할수록 증가하는 경향을 나타내었다. 따라서, 제형 내 oil tomposition을 변화시킴으로써 phenoxyethanol의 사용량을 줄일 수 있을 뿐만 아니라, 피부 투과를 감소시켜 보다 피부 자극이 적은 저자극 방부시스템 개발이 가능하리라 보여 진다. 첨가하여 제조한 curd yoghurt는 저장성과 관능적인 면에서 우수한 상품적 가치가 인정되는 새로운 기능성 신제품의 개발에 기여할 수 있을 것으로 사료되었다. 여자의 경우 0.8이상이 되어서 심혈관계 질환의 위험 범위에 속하는 수준이었다. 삼두근의 두겹 두께는 남녀 각각 $20.2\pm8.58cm,\;22.2\pm4.40mm$으로 남녀간에 유의한 차이는 없었다. 조사대상자의 식습관 상태는 전체 대상자의 $84.4\%$가 대부분이 하루 세끼 식사를 규칙적으로 하고 있었으며 식사속도는 허겁지겁 빨리 섭취하는 경우가 남자는 $31.0\%$, 여자는 $21.4\%$로 나타났고 이들을 제외한 나머지 사람들은 보통 속도 혹은 충분한 시간을 가지고 식사를 하였다. 평소 식사량은 조금 적게 혹은 적당하게 섭취하는 사람이 대부분이었으며 남자가 여자보다는 배부르게 먹는 경 향이 유의적으로 높았다(p<0.05). 식사는 혼자 하는 경우가 남자

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LiDAR Chip for Automated Geo-referencing of High-Resolution Satellite Imagery (라이다 칩을 이용한 고해상도 위성영상의 자동좌표등록)

  • Lee, Chang No;Oh, Jae Hong
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.32 no.4_1
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    • pp.319-326
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    • 2014
  • The accurate geo-referencing processes that apply ground control points is prerequisite for effective end use of HRSI (High-resolution satellite imagery). Since the conventional control point acquisition by human operator takes long time, demands for the automated matching to existing reference data has been increasing its popularity. Among many options of reference data, the airborne LiDAR (Light Detection And Ranging) data shows high potential due to its high spatial resolution and vertical accuracy. Additionally, it is in the form of 3-dimensional point cloud free from the relief displacement. Recently, a new matching method between LiDAR data and HRSI was proposed that is based on the image projection of whole LiDAR data into HRSI domain, however, importing and processing the large amount of LiDAR data considered as time-consuming. Therefore, we wmotivated to ere propose a local LiDAR chip generation for the HRSI geo-referencing. In the procedure, a LiDAR point cloud was rasterized into an ortho image with the digital elevation model. After then, we selected local areas, which of containing meaningful amount of edge information to create LiDAR chips of small data size. We tested the LiDAR chips for fully-automated geo-referencing with Kompsat-2 and Kompsat-3 data. Finally, the experimental results showed one-pixel level of mean accuracy.

A Comparison on Dry Matter Yield and Feed Value of Winter Forage Crops Cultivated after Rice Harvest in the Central Inland Region (중부내륙지방에서 벼 수확 후 재배한 월동 사료작물들의 생산성 및 사료가치 비교)

  • Lee, Sang Moo
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.39 no.1
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    • pp.31-38
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    • 2019
  • This study was carried out to investigate the growth characteristics, yield, and chemical compositions of winter forage crops cultivated after rice harvest in the central inland region. The experimental design was arranged in a randomized block design with three replications. The treatments consisted of 4 species (Rye, Oat, Triticale and Italian ryegrass), and varieties were "Marton", "Donghan", "Shinyoung" and "Kowinnearly", respectively. As a result, dry matter yield and TDN yield were higher in rye than in the other winter forage crops, and lowest in Oat(p<0.05). Crude protein and crude fat content were significantly higher in Italian ryegrass(p<0.05). However, Crude ash and ADF content did not show significant difference among winter forage crops. NDF content was higher in order of Rye > Triticale > Oat > Italian ryegrass(p<0.05). TDN and total amino acid content (EAA+NEAA) were higher in order of winter forage crop with high crude protein content (Italian ryegrass > Oat > Triticale > Rye). Total mineral content was higher in order of Rye > Triticale > Oat > Italian ryegrass(p<0.05), and total free sugar content was higher in order of Oat > Italian Ryegrass > Triticale > Rye(p<0.05). Compared with the results above, Italian ryegrass and Oat are high in crude protein, TDN, amino acid and free sugar content. Rye and Triticale have the merit that feed value is decreased but high yield(dry matter and TDN yield) can be maintained. Therefore, it is advantageous to grow Rye and Triticale as winter forage crops after rice harvest in the central inland region.

A PDA WebDAV Client Based on .NET Compact Framework (.NET Compact Framework 기반의 PDA WebDAV 클라이언트)

  • Park Jin-Ho;Kim Dong-Ho;Lee Hong-Chang;Lee Myung-Joon
    • Proceedings of the Korean Information Science Society Conference
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    • 2006.06d
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    • pp.82-84
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    • 2006
  • WebDAV는 IETF 표준으로서 웹상에서 분산 저작을 지원하도록 HTTP를 확장한 프로토콜이다. WebDAV 클라이언트는 WebDAV 프로토콜을 이용하여 WebDAV 서버자원을 관리하는 도구이다. 현재 WebDAV 기반의 자원 관리가 널리 사용되면서 무선 네트워크 환경에서 동작하는 클라이언트의 필요성이 증대되고 있다. 본 논문에서는 무선 네트워크를 지원하는 PDA에서 동작하는 WebDAV 클라이언트의 구현에 대하여 기술한다. 개발된 클라이언트는 간결한 사용자 인터페이스를 제공하며 WebDAV의 기본 메소드를 지원한다. 사용자는 인터페이스와 기본 메소드를 이용하여 WebDAV 서버에 자원을 등록하고, 서버 자원에 대한 열람, 검색, 이동 및 삭제를 할 수 있으며, 서버 자원을 내려 받을 수 있다. 본 클라이언트는 PDA에서 동작하기 위해서 .NET Compact Framework를 기반으로 구현 하였으며 관련 에뮬레이터를 이용하여 실험하였고 이를 실제 PDA장치인 'HP iPAQ hx2700'에 이식하여 검증하였다.

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A Study on Shooting Techniques of 3 Dimensional Stereoscopic Rider Movie (3D입체 라이더영상의 촬영기법에 관한 연구)

  • Choi, Won-Ho;Kim, Cheeyong
    • Journal of Korea Multimedia Society
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    • v.17 no.2
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    • pp.257-265
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    • 2014
  • 3 dimensional stereoscopic is attracting new attention as it simulates human eye and related hardware advances. However, 3 dimensional stereoscopic requires more time and effort than 2D movie because it uses 2 cameras or 2 lenses. This study analyzed the merits and demerits of the system producing rider movie using 3 dimensional stereoscopic shooting techniques and searched a way to produce better 3 dimensional stereoscopic rider movie which meets the producing intention by applying elements required to create effective 3 dimensional stereoscopic effect. According to the study result, a light-weight integrated system was effective to create 3 dimensional stereoscopic rider movie because of its genre characteristic. In the post-production of the work, location shooting considered of time re-mapping, color correction and graphic element addition was required.