• 디지털융복합연구
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• 제12권6호
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• pp.407-414
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• 2014

이 연구의 목적은 광감작제인 라다클로린과 포토프린을 발광다이오드에 접목하여 포도알균의 광역학치료 효과를 평가하고자 하였다. 실험방법은 황색포도알균이나 표피포도알균의 $1{\times}10^5CFU/ml$이 되게 균주부유액을 준비하였고, 광감작제(포토프린 또는 라다클로린)를 1.25, 2.5, 5, $10{\mu}g/ml$가 되도록 희석하였다. 균주희석액은 에너지밀도 각각 $14.4J/cm^2$와 $19.8J/cm^2$로 630 또는 670 파장 발광다이오드 빛을 조사하였다. 황색포도알균과 표피포도알균의 집락형성수는 포토프린 $5{\mu}g/ml$ 농도에서 33, 50개의 집락이 각각 형성되었고, 두 세균 모두 라다클로린 $5{\mu}g/ml$ 농도에서는 완전한 살균을 나타냈다. 유세포분석에 의한 형광강도는 정상세포보다 죽은세포에서 증가를 보였다. 투과전자현미경의 사진에서는 세포막의 손상과 부분적으로 세포형태의 파괴가 관찰되었다. 이 결과로 포토프린과 라다클로린을 이용한 광역학치료는 항균치료의 새로운 방법이 될 수 있음을 제의한다.

• 대한방사선기술학회지:방사선기술과학
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• 제44권2호
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• pp.141-146
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• 2021

This study aimed to explore whether photodynamic therapy using Radachlorin and diode laser is an effective inhibitor of Candida albicans. Suspensions of Candida albicans were obtained, inoculated in petri dishes with Radachlorin, and incubated for 30 min. Then, the laser light of a diode laser was irradiated at at energy densities of 3 J/cm², 5 J/cm², 7 J/cm². As a result, Candida albicans showed a killing rate of 91.5% at an energy density of 7 J/cm². This study found that photodynamic therapy using a Radachlorin and diode laser was effective for the inhibition of Candida albicans.

• 대한방사선기술학회지:방사선기술과학
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• 제45권2호
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• pp.165-170
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• 2022

Staphylococcus aureus is a major pathogen that causes clinical infections in humans and can also cause massively colonized in lesion skin, particularly in atopic dermatitis patients. This study investigated the effects of photodynamic inactivation with radachlorin and diode laser irradiation on the viability of S. aureus in vitro and assessed the effects of the dose of laser transmission. In the PDI group, 5 𝜇L of S. aureus suspension and 5 𝜇L of radachlorin were inoculated in a 55 mm petri dish (63.6 cm²). The samples were placed in a 37° incubator for 30 min and then irradiated with light (660 nm diode laser). After laser irradiation, the cells were stored for 24 h at 37° in an incubator with 5% CO2, and the number of colonies was counted. All CFU/mL of S. aureus were reduced by diode laser in the presence of radachlorin, with a killing rate of 87.9% at an energy dose of 9 J/cm². This study contribute to treat colonized with S. aureus in atopic dermatitis patients and wound infections by providing information on the optimal dose of laser transmission using PDI to eliminate S. aureus.