• Journal of Life Science
• /
• v.30 no.11
• /
• pp.1012-1020
• /
• 2020

Remicade is a therapeutic biosimilar natural antibody in which the mouse variable domain has been linked to the human constant domain. It is a chimeric monoclonal antibody specific to tumor necrosis factor-alpha (TNF-α) and has been developed for the treatment of rheumatoid arthritis. To investigate the biological activity of the Remicade antibody, we carried out a bioinformatics study using a protein data bank to characterize the TNF-α antigen binding mechanism of the Remicade natural antibody. Because the production of the Remicade antibody is often limited by genetic instability of the natural antibody-producing cell, we generated a Remicade single-chain variable domain fragment antibody (Remicade) in which a heavy chain variable domain (VH) is joined with a light chain variable domain (VL) by a polypeptide linker. Furthermore, Remicade was fused to a leucine zipper (RemicadeScZip) for higher production and higher antigen-binding activity than Remicade. The Remicade and Remicade ScZip were expressed in Escherichia coli and purified by a Ni⁺-NTA-agarose column. As expected, the purified proteins had migrated as 28.80 kDa and 33.96 kDa in sodium dodecyl sulfate-polyacrylamide electrophoresis. The TNF-α antigen binding activity of Remicade was not observed by ELISA and western blot. In contrast, RemicadeScZip showed antigen-binding activity. Additional bio-layer interferometry analysis confirmed the antigen-binding activity of RemicadeScZip, suggesting that the leucine zipper stabilized the folding of RemicadeScZip in a denatured condition and improved the TNF-α antigenbinding activity.

• Spatial Information Research
• /
• v.17 no.3
• /
• pp.397-404
• /
• 2009

To solve the problems of distribution and quality on land gravity data, airborne gravity survey was performed in 2008 obtaining the airborne gravity data with accuracy of 1.56mGal. Since airborne gravity data is the obtained at the flight height, it is necessary to convert the airborne gravity data to the surface to combine various gravity data and compute precision geoid. In addition, Stokes' integral radius, Stokes' kernel and the radius of terrain effect computation should be optimally determined to calculate precision geoid. In this study, we made an effort to decide the optimal parameters based on the distribution and the characteristic of gravity data. Then, two geoid models were calculated using the selected parameters and the difference of geoid was calculated with mean of -16.95cm and the standard deviation of ${\pm}8.50cm$. We consider that this difference is due to the distribution and errors on the gravity data. For future work, the study on the effect of geoid with newly obtained land gravity data ship-borne gravity data and GPS/Leveling data should be conducted. Furthermore, the study on the downward continuation and terran effect calculation should be studied in detail for better precision geoid construction.

• Archives of design research
• /
• v.15 no.3
• /
• pp.5-14
• /
• 2002

The amusement design of a large amusement park, including theme park, largely consists of attraction design, the main facilities, and auxiliary facilities design. The purpose of this study was to discuss the physical motion characteristics of major ride, a recently highlighted representative ride attraction, modern people's thirst for stronger stimuli, and some of the right future directions for amusement design. In theme park, there is an growing trend for rides, especially major rides, to be more thrilling, and Newton's Law motion is basically applied. The rides we can often see provides a thrilling experience that is different from that offered by vehicles we use everyday, due to a variety of speed change, namely of acceleration. Every entertainment facilities, including ride attractions, are externally cutting edge with the use of new technology. but internally, poor theme or concept not only makes it difficult to function properly as attraction but poses a severe threat to theme park survival itself. Theme park will be able to exist as a comprehensive entertainment entity, when attractions are designed to have a perfect harmony among story, method and environment with cultural universality.

• Journal of Navigation and Port Research
• /
• v.29 no.5 s.101
• /
• pp.395-402
• /
• 2005

The most important point when we engage on waterway dredging work is supplying safe navigational passage to the vessels underway by narrowing dredge work area and removing submerged dangers. In order to meet this end it is necessary to use auxiliary equipment for shifting actively and mooring and adopt automation of dredging work by integrating information on real time position, dredging depth, and work information. The dredger with a spud control system in this study, by the way, is able to employed on continuous dredging work with the narrowest working area allowing wide and safe passages to vessels underway, by moving the dredger to the working zone with the spud controlled automatically. Furthermore, it has been improved definitely compared with the existing dredging process management system such that it shows the track of spud and working depth on the electronic navigation chart of window, together with the final outcome of dredging work. The test dredging work at the entrance of Busan North Port for system evaluation showed that actual working time available was twice of the one by the existing anchor system, and that it reduced $38\%$ of time for preparation work and one man power.

• Economic and Environmental Geology
• /
• v.42 no.5
• /
• pp.495-500
• /
• 2009

Actinide sorption to the geological materials can reduce the mobility and bioavailability of radionuclides released to the environment through the development of nuclear weapons and nuclear energy. Under circumneutral pH conditions, actinide sorption can be enhanced by phosphate anions sorbed on oxide mineral surfaces as indicated by the sorption of trivalent lanthanide ions ($Ln^{3+}$), the chemical analog for trivalent actinide ions ($Ac^{3+}$). In this paper, we examined a ternary sorption system of trivalent europium ions ($Eu^{3+}$) sorbed onto boehmite (${\gamma}$-AlOOH) surfaces pre-sorbed with phosphate anions (${PO_4}^{3-}$), using extended X-ray absorption fine structure (EXAFS) spectroscopy. In the Eu-$PO_4$-boehmite ternary sorption system, $EuPO_4$ surface precipitates were formed as implicated by Eu $L_{III}$-edge EXAFS spectroscopy. Phosphorus K-edge EXAFS fingerprinting indicated a bidentate mononuclear surface complex formation of phosphate sorbed on boehmite surfaces as well as $EuPO_4$ surface precipitate formation.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
• /
• v.26 no.1
• /
• pp.51-61
• /
• 2008

The determination of precise geoid model for the Jeju island is needed to minimize the effect of different vertical datums. This study describes the development of gravimetric geoid model referred to GRS80 reference surface for the area of Jeju island. We used ECM96 up to degree and order 360 as a reference model and added the terrain and the residual gravity effects to the reference model. After then 17 GPS/Levelling data were used to correct the difference between the GPS/Levelling-derived geoid heights and gravimetric geoid heights. The least square collocation was applied to derive the correction and the grid values. The final precise geoid model(Jeju_GEOID07) that consist of $0.75'{\times}1'$(about $1.4km{\times}1.5km)$ grid interval was obtained in the region of $33^{\circ}{\sim}33.8^{\circ}N$ and $125.8^{\circ}{\sim}127.2^{\circ}E$. Concerning this works, the precise geoid for the Korean peninsula should be determined by integrating the different geoid developed for the peninsula and Jeju island. It is also need to integrate the vertical datum using long-term tide and GPS observations.

• Journal of the Institute of Electronics Engineers of Korea SC
• /
• v.40 no.3
• /
• pp.215-224
• /
• 2003

This paper presents an electronic ballast using a novel complex modulation method for the metal halide lamp. The proposed modulation method, which has a modulating signal of swept complex frequency, can eliminate the acoustic resonance more effectively than the conventional modulation method, which has a modulating signal of constant frequency. For the purpose of future application specific integrated circuits (ASIC). the controller of the proposed ballast has been designed only with erasable programmable logic devices (EPLDs), but without a microprocessor. In this paper, detailed proposed modulation schemes are described and experimental results on the proto type 150W metal halide lamp ballast with the proposed modulation method ate discussed.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
• /
• 2009.06a
• /
• pp.71-72
• /
• 2009

칼코게나이드(chalcogenide)계 비정질 반도체는 그들의 독특한 광학적, 전기적 특성 때문에 활발한 연구가 진행되고 있다. 비정질 Se는 유연한 원자구조로 인해 가장 먼저 광학적으로 상용화되었으며, 응용성이 매우 큰 반도체 재료중 하나로 Ge-Se와 같이 Se를 기본으로 한 칼코게나이드 유리 반도체가 주목받고 있다. 따라서 본 연구에서는 비정질 칼코게나이드 $Ge_xSe_{100-x}$(x=25,95) 조성에 대한 광학적 특성을 연구 하였다. 시료는 5N의 순도를 갖는 Ge, Se 물질을 준비하고 조성비에 맞추어서 석영관에 진공 봉입한 후 용융 혼합하여 $Ge_{25}Se_{75}$와 $Ge_{95}Se_5$조성의 두 가지 비정질 벌크를 제작하였다. 열증착 방법으로 유리 기판위에 박막을 제작하였고 UV-vis-NIR spectrophotometer를 사용하여 투과도를 측정하였다. 측정한 스펙트럼을 이용하여 Swanpoel method로 굴절률을 계산하고 특성을 분석하였다.

• Journal of the Mineralogical Society of Korea
• /
• v.16 no.2
• /
• pp.191-198
• /
• 2003

The ochreous precipitates, reddish brown and brownish yellow in color, are pre- cipitated in the stream bottom of acid mine drainage (AMD) in the Donghae coal mine area. X-ray diffraction analysis shows that the reddish brown precipitate consists mainly of ferrihydrite with small amount of goethite, while the brownish yellow precipitate of schwertmannite. Thermal experiments show that ferrihydrite and schwertmannite partially convert to poorly-crystallized hematite at $400^{\circ}C$ and to well-crystallized hematite at $700^{\circ}C$.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
• /
• v.16 no.1
• /
• pp.20-26
• /
• 2002

HID(high intensity discharge)lamps include high pressure mercury lamp, high pressure sodium lamp and metal halide lamp. Metal halide lamps have been considered to be one of the most erective light sources. These lamps have good efficiency, good color rendering and good focusing capability. The objective of this research is to alum on the metal halide lamp(MH70W) by the developed electronic ballast with free voltage input(AC 100∼277V). The developed electronic ballast consists of a free voltage input converter, a flyback converter ＆ half-bridge inverter, ignitor ＆ protector and control1er. Experimental results show that developed electronic ballast turns on the metal halide lamp(MH70W) with free voltage input(AC 100∼277V) very well.