• Food Science of Animal Resources
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• v.27 no.1
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• pp.35-41
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• 2007

This study was conducted to determine the effect of pH adjustment and addition of sodium chloride (NaCl) on quality characteristics of pork leg surimi. The pork leg surimi was manufactured with one of following pH 3.0 or pH 11.0 adjustment and contained 2% NaCl or not; C (Alaska pollack surimi, two times washing, 0% NaCl), T1 (pork leg surimi pH 3.0 adjustment, 0% NaCl), T2 (pork leg surimi, pH 3.0 adjustment, 2% NaCl), T3 (pork leg surimi, pH 11.0 adjustment 0% NaCl), T4 (pork leg surimi, pH 11.0 adjustment, 2% NaCl). The $L^*$ and W values was increased with increasing of pH value, but the $a^*$ and $b^*$ values lower. The W values were higher (p<0.05) as addition of NaCl, but $a^*$ and $b^*$ values were lower. In textural properties, the cohesiveness was increased with increasing of pH value, however with the exception of springiness all traits were higher (p<0.05) as addition of NaCl. The breaking force and gel strength was increased with increasing of pH value. The breaking force, gel strength and breaking force u deformation were higher as addition of NaCl, but shear force lower. In sensory evaluation, the tenderness was increased with increasing of pH value and all traits of sensory evaluation also were higher (p<0.05) as addition of NaCl. There were no differences in interaction of pH adjustment and without or with NaCl on color, textural properties, breaking force, deformation, gel strength, shear force and sensory evaluation of pork leg surimi. The color, textural and physical characteristics and sensory evaluation ot T2 and T4 were similar to treatment C. Therefore, the pH 11.0 and 2% NaCl addition on process of manufacture of pork leg surimi would be recommended.

• Clinical and Experimental Reproductive Medicine
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• v.34 no.2
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• pp.95-106
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• 2007

Objective: We previously identified differentially expressed genes (DEGs) between germinal vesicle (GV) and metaphase II (MII) mouse oocyte. The present study was accomplished as a preliminary study to elucidate the role of ribose 5-phosphate isomerase A (Rpia), the essential enzyme of the pentose phosphate pathway (PPP), in oocyte maturation. We observed expression of Rpia in the mouse and porcine oocytes. Methods: Expression pattern of the 11 MII-selective DEGs in various tissues was evaluated using RT-PCR and selected 4 genes highly expressed in the ovary. According to the oocyte-selective expression profile, we selected Rpia as a target for this study. We identified the porcine Rpia sequence using EST clustering technique, since it is not yet registered in public databases. Results: The extended porcine Rpia nucleotide sequence was submitted and registered to GenBank (accession number EF213106). We prepared primers for porcine Rpia according to this sequence. In contrast to the oocyte-specific expression in the mouse, Rpia was expressed in porcine cumulus and granulosa cells as well as in oocytes. Conclusion: This is the first report on the characterization of the Rpia gene in the mouse and porcine ovarian cells. Results of the present study suggest that the mouse and porcine COCs employ different mechanism of glucose metabolism. Therefore, the different metabolic pathways during in vitro oocyte maturation (IVM) in different species may lead different maturation rates. It is required to study further regarding the role of Rpia in glucose metabolism of oocytes and follicular cell fore exploring the regulatory mechanism of oocyte maturation as well as for finding the finest culture conditions for in vitro maturation.

• Journal of Radiation Protection and Research
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• v.38 no.4
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• pp.202-207
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• 2013

Bioimpedance involves a lot of information related to living tissue. If there is alteration in bio tissue, its electrical characteristics also change. It is to study electrical characteristics of pork tenderlion in using a HP-4194A Impedance/Gain-phase analyzer instrument and electrical characteristics changes by graded radiation exposure dose. The results were as follow 1. Electrical characteristics of pork tenderlion in repeated measurement had high precision within ${\pm}5$% of coefficiency of variability. 2. During the measurement impedance absolute value and phase alteration did not show statistically significant difference.(p>0.05) 3. While impedance phase of electrical characteristics associated with frequency change was almost stable, impedance absolute value was in inverse proportion to frequency that means high inverse correlation of -0.096(r). 4. Impedance absolute value dropped in radiation exposure dose. The alteration of the value did not show statistically significant difference in 1 Gy, 2 Gy and 4 Gy.(p>0.05) However in radiation exposure dose of 10 Gy, the decrease of impedance absolute value was significantly different.(p<0.05) 5. Impedance phase according to radiation exposure dose change did not show statistically significant difference in 1 Gy, 2 Gy, 4 Gy, and 10 Gy(p>0.05).

• Korean Journal of Veterinary Research
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• v.37 no.2
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• pp.339-347
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• 1997

A cytopathogenic virus was isolated from the brain tissues of pig showing ataxia. The biophysical, morphological and serological assay showed that the isolate belongs to a coronavirus. The differential identification of the isolate with monoclonal antibodies against A and X sites of transmissible gastroenteritis virus indicated that the virus has a characteristics of porcine respiratory coronavirus. The RT-PCR on nucleocapsid region of TGEV also showed that the isolate has the same conserved sequence. The diverse pathogenesis of PRCV and its implication in field were discussed.

• Journal of Yeungnam Medical Science
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• v.5 no.2
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• pp.1-8
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• 1988

위장관에서 분비되는 많은 국소호르몬 중에서 이미 널리 알려진 것을 외에 구조적인 공통점을 가진 새로운 group인 pancreatic polypeptide family에 대한 정보를 요약하여 보았다. PP, NPY 및 PYY는 그 구조적 유사점들에도 불구하고 분비되는 장소가 판이하여, PP는 위와 췌장의 국소호르몬으로서, NPY는 중추 및 말초신경의 신경충동전달체로서, 또 PYY는 하부위장관 점막에서부터 분비되어 전신 호르몬처럼 작용하고 있다. 이들 NPY와 PYY의 혈관수축작용과 혈압상승작용은 우리의 흥미를 끌기에 충분하다. 특히 PYY는 주로 하부장관 특히 결장과 직장의 분비조직에서 많이 발견된다는 점과 돼지의 뇌혈관을 수축시켰다는 점이 주목할 만한 것이다. 우리나라에는 많은 고혈압 환자가 있으며, 고혈압을 비롯한 여러가지 원인에 의한 뇌졸중(뇌출혈 포함) 환자도 많이 발생하고 있다. 민간의 속설에 의하면 출혈성 뇌졸증이 기온이 낮은 계절에 보다 자주 발생하는 경향이 있으며, 이른아침 화장실에서의 배변시 자주 발생한다고 한다. 물론 이러한 속설을 뒷받침할 만한 자료가 없으므로 학문적 견지에서의 신빙성 여부를 논할 가치는 없겠으나, 배변시 비교적 강한 자극을 받고 활발한 운동을 하는 결장과 직장에 PYY의 분비세포가 집결되어 있다는 점과 PYY의 뇌혈관수축작용 및 혈압 상승작용 등을 고려해 볼 때, 출혈성 뇌졸증과 PYY의 상관관계는 추구해볼 만한 사항이라 사료된다.

• Journal of the Korean Crystal Growth and Crystal Technology
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• v.33 no.2
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• pp.71-77
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• 2023

Absorbable periodontal tissue regeneration barrier membranes (total 6; domestic 4; import 2) were comparatively analyzed. In the case of the xenograft barrier membrane, the collagen product had excellent tensile strength but low strain, and the porcine pericardial membrane had good mechanical properties, but its thickness was too thick to control. The synthetic PLLA membrane manufactured by the electrospinning had a relatively low water absorption capacity. However, the hybrid barrier membrane was able to control mechanical properties and biocompatibility through proper mixing of synthetic polymer and natural polymer. DA02 (PLLA/gelatin), a newly developed hybrid absorbable periodontal tissue regeneration membrane that is entirely dependent on imports, can be applied to an absorbable periodontal tissue regeneration barrier membrane due to suitable mechanical properties and biocompatibility.

• Journal of Chest Surgery
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• v.41 no.6
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• pp.679-686
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• 2008

Background: Cellular remnants in the bioprosthetic heart valve are known to be related to a host's immunologic response and they can form the nidus for calcification. The extracellular matrix of the decellularized valve tissue can also be used as a biological scaffold for cell attachment, endothelialization and tissue reconstitution. Thus, decellularization is the most important part in making a bioprosthetic valve and biological caffold. Many protocols and agents have been suggested for decellularization, yet there ave been few reports about the effect of a treatment with hypotonic solution prior to chemical or enzymatic treatment. This study investigated the effect of a treatment with hypotonic solution and the appropriate environments such as temperature, the treatment duration and the concentration of sodium dodecylsulfate (SDS) for achieving proper decellularization. Material and Method: Porcine aortic valves were decellularized with odium dodecylsulfate at various concentrations (0.25%, 0.5%), time durations (6, 12, 24 hours) and temperatures ($4^{\circ}C$, $20^{\circ}C$)(Group B). Same the number of porcine aortic valves (group A) was treated with hypotonic solution prior to SDS treatment at the same conditions. The duration of exposure to the hypotonic solution was 4, 7 and 14 hours and he temperature was $4^{\circ}C$ and $20^{\circ}C$, respectively. The degree of decellularization was analyzed by performing hematoxylin and eosin staining. Result: There were no differences in the degree of decellularization between the two concentrations (0.25% 0.5%) of SDS. Twenty four hours treatment with SDS revealed the best decellularization effect for both roups A and B at the temperature of $4^{\circ}C$, but there was no differences between the roups at $20^{\circ}C$. Treatment with hypotonic solution (group A) showed a better ecellularization effect at all the matched conditions. Fourteen hours treatment at $4^{\circ}C$ ith ypotonic solution prior to 80S treatment revealed the best decellularization effect. The treatment with hypotonic solution at $20^{\circ}C$ revealed a good decellularization effect, but his showed significant extracellular matrix destruction. Conclusion: The exposure of porcine heart valves to hypotonic solution prior to SDS treatment is highly effective for achieving decellularization. Osmotic treatment with hypotonic solution should be considered or achieving decellularization of porcine aortic valves. Further study should be carried out to see whether the treatment with hypotonic solution could reduce the exposure duration and concentration of chemical detergents, and also to evaluate how the structure of the extracellular matrix of the porcine valve is affected by the exposure to hypotonic solution.

• Journal of Food Hygiene and Safety
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• v.8 no.1
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• pp.25-35
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• 1993

This study is conducted to deyelop more conyenient simultaneous determination method by HPLC for mixed antimicrobial agents (sulfamerazine; SMR, sulfamethazine; SMT, sulfamonomethoxine; SMMX, sulfadimethoxine; SDMX, sulfaquinoxaline; SQX, furazolidone; FZ, zolene; ZOL and ethopabate; EPB in muscles of boline, pork and chicken. The drugs were extracted by dichloromethane and water. The extract, after solyent eyaporation, was partitioned in hexane/water and water/dichloromethane. The dichloromethane layer was filtrated with anhydrous $Na_5S0_4\;in\;_3G_3$ glass filter and was eyaporated to dryness. The residue was dissoIYed in mobile phase. The test solution analyzed by HPLC. The chromatograpic conditions were as follows; Column-Spheri 5 RP-8($4.6{\times}220,\;5\;{\mu}$), Wayelength-270 nm, Mobile phase-acetonitrile: 0.005 M oxalic acid (22 : 78). The ayerage recoyeries of drugs from muscles of boline, pork and chicken spiked standard solution were approximately 74~99%, 73~99% and 75~96%, respectiyely. The limits of detection were 5 ppb for SMR, SMT, SMMX, SDMX and EPB, and 8 ppb for SQX, FZ and ZOL.

• Journal of Veterinary Clinics
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• v.28 no.1
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• pp.1-6
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• 2011

We are aim to evaluate antimicrobial effects of the extract of Galla rhois (GR) on the health status and performance of growing and finishing pigs. This study was conducted on the growing and finishing pigs (n = 200) for 130 days in a swine husbandry. The animals were divided with two groups; GR treated group (n = 100) and commercial diet feeding group (n = 100). GR treated animals had provided with commercial diet adding the extract of GR as 0.2%. During the study period, we compared clinical signs, weight increase rate, diet consumption amount, fecal scores, gross findings, necropsy findings, histopathological findings between the treated group and non treated group. After necropsy, bacteria isolation and PCR analysis were conducted with the clinical samples. As the results of this long-term clinical trial, GR showed the antimicrobial effects on respiratory disease and diarrhea. We were identified that GR had the anti-bacterial and anti-viral effects.

• Korean Journal of Veterinary Research
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• v.33 no.3
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• pp.429-442
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• 1993

The cytotoxic effects of S hyodysenteriae strain B 204 on the mucosal surface were studied in surgically prepared ligated colonic loops in two male convenitonal mixed-breed pigs. In each one of four loops was inoculated with either sterile trypticase soy broth(TSB) of Serpulina, filtrate of Serpulina TSB culture. washed Serpulina cells or whole culture of Serpulina. Mucosal tissues were examined by transmission and scanning electron microscopy 24 and 48 hours after inoculation(p.i.). The filtrate did not induce any significant effect on the mucosal surface. The washed cells produced early lesions similar to those caused by the whole culture. These observations suggest that cytotoxins of the culture do not play a significant role in invasion of the epithelium in this experimental infection. The possible role of toxins associated with the organism at the site of interaction with the epithelial cells was not elucidated.