Induced breeding and indoor rearing of the catfish, Silurus asotus were performed. Ovulation was induced by injection of 5 mg dried carp pituitary per kg body weight. Fertilization rates were $81.5\~98.0{\%}$ and hatching success was $67.0\~82.0{\%}$. There were no significant differences of daily growth rate (DGR) and food conversion ratio (FCR) between the groups fed extruded eel pellet and extruded carp pellet. No significant differences in DGR and FCR were also found between $3{\%}$ and $4{\%}$ daily feeding rates. DGR and FCR of small fry were slightly affected by dissolved oxygen (DO) level in aquaria. When the DO level maintained at $4.5\;mg/{\ell}$, the best result was obtained and DGR and FCR were $5.7{\%}$ and 0.65, respectively. At the higher level of DO, $5.5\;mg/{\ell}$, DGR and FCR were $5.52{\%}$ and 0.77, respectively, whereas at the lower level of DO, $3.5\;mg/{\ell}$, the DGR was decreased to $3.45{\%}$ and FCR was increased up to 1.01.
Kim, Dong-Soo;Lee, Sang-Myeong;Huh, He-Soon;Park, Nam-Chang;Park, Chung-Gyoo
Korean journal of applied entomology
/
v.48
no.4
/
pp.527-533
/
2009
This study was performed to investigate the escape of pine wood nematode (PWN), Bursaphelenchus xylophilus, from two vector species (Monochamus alternatus and M. saltuarius) through oviposition and feeding behavior. First, we checked number of PWNs escaped from M. alternatus emerged from three different cases of pine logs. In case A, healthy pine trees were cut into logs and left in pine forest infected with PWN. In case B, healthy pine trees were cut into logs, left in large screen cage, and let them oviposited by M. alternatus emerged from pine trees infested with PWN. In case C, pine trees which were harboring M. alternatus were cut into logs, and PWN was inoculated artificially. The M. alternatus adults emerged from the above three cases of pine logs were checked in the next year to know how many PWN they were harboring in their bodies. The percentages of M. alternatus harboring PWN (18.3 and 15.6%, respectively) and number of nematodes per vector ($5,713.1{\pm}9,248.3$ and $2,034.1{\pm}4,746.8$ PWNs, respectively) in case A and B logs are similar to each other. However, the percentage and the number in case C (38.3% and $20,083.1{\pm}32,188.3$ PWNs) were higher than those of case A and B. Among 52 M. alternatus adults harboring PWN from all the three cases, 20 adults (38.5%) were harboring more than 5,000 PWNs per beetle. And these 20 adults were harboring 97.9% of the total PWNs in 52 adults. Second, we checked the daily escape of PWNs from M. alternatus and M. saltuarius collected at pine forest infested with PWN. The PWN escaped from their vector body for $34.9{\pm}12.4$ days for M. alternatus, and for $23.9{\pm}16.2$ days for M. saltuarius, reaching at peak escape during the 2nd week of emergence of the two vector species. A 44.5 and 47.2% to the total PWNs escaped from vector body within 2 weeks of vector emergence for M. alternatus and M. saltuarius, respectively. The number of PWNs escaped from each vector was $3,570.6{\pm}5,189.2$ and $1,556.2{\pm}1,710.3$ for M. alternatus and M. saltuarius, respectively.
Kang Ji-Hoon;Kim Nam-Hoon;Song Yong-Sun;Park Kye-Hun
The Journal of the Petrological Society of Korea
/
v.15
no.2
s.44
/
pp.49-59
/
2006
This study clarifies the deformation history of Precambrian metamorphic rocks of Sobaegsan Massif (Wonnam Formation, Pyeonghae granite gneiss, Hada leucogranite gneiss) in Giseong-myeon area, Uljin-gun, Korea. It is based on the geometric and kinematic features and the developing sequence of multi-deformed rock structures. It also reviews the extension of Yecheon Shear Zone and the relative occurrence time of each deformation phase from previous researches. It suggests that the geological structure was formed at least through five phases of deformation after formation of their gneissosity or schistosity. (1) The first phase of deformation took placed under compression of ENE-WSW direction, forming NNW trending regional foliation and very tight isoclinal fold. The general trend of gneissosity or schistosity is inferred to be ENE before the first phase of deformation, being rearranged into NNW by the isoclinal folding. (2) The second phase of deformation formed ENE trending regional foliation and tight, isoclinal, rootless intrafolial folds under compression of NNW-SSE direction [occurrence time: after deposition (Permian age) of Dongsugok Formation, Pyeongan Croup, Janggunbong area]. (3) The third phase of deformation occurred by dextral ductile shearing on the regional foliation, forming stretching lineation of ENE trend and S-C mylonitic structure (after intrusion of Hesozoic homblende granite, Sangunmyeon area-before intrusion of Mesozoic Chunyang granite, Janggunbong area). (4) The fourth phase occurred under (E)NE-(W)SW compression, forming (N)NW trending open fold. (5) The fifth phase took place under N-S compression, forming NNE and NNW trending conjugate strike-slip faults, E-W trending thrust-slip faults, and drag folds related to these fault movements. The deformed structures of fourth and fifth phases result from tectonic movement associated with the developing of the Gyeongsang Basin in Cretaceous age, and it partially rearranged the general ENE trend of the regional foliation in the study area. It also suggests that the Yecheon Shear Zone of E-W trending extends into this area but the ductile shear deformation is weakly developed.
Yeon S. H.;Son D. S.;Jean H. J.;Choi S. H.;Kim I. C.;Park C. S.;Lee K. S.
Journal of Embryo Transfer
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v.19
no.3
/
pp.265-273
/
2004
This study was carried out to examine the effects of fertilization media and sperm concentration on in vitro fertilization (IVF) and development (IVD) of porcine oocytes matured in vitro. Cumulus-oocyte complexes (COCs) were collected from antral follicles of porcine ovaries collected from abattoir, and were matured in vitro in modified NCSU-23 (mNCSU-23) supplemented with 10% porcine follicular fluid (pFF). After the fertilization by experimental scheme, putative embryos were developed in vitro in NCSU-23. The results are as follows. When the oocytes were fertilized in vitro in modified TBM or modified TLP-PVA by 1 ${\times}$10$^{5}$ sperm/$m\ell$, all of the fertilization parameters were not significantly different between two media. Subsequently, as these putative embryos were developed in vitro in NCSU-23, the percentage of oocytes cleaved and of blastocysts were not different between two media, either. When the oocytes were fertilized in vitro in mTBM by 5${\times}$10$^4$, 1${\times}$10$^{5}$ or 5${\times}$10$^{5}$ sperm/$m\ell$, all of the fertilization parameters were significantly (P<0.05 or P<0.01) increased as sperm concentration was elevated. Subsequently, as these putative embryos were developed in vitro in NCSU-23, the percentage of oocytes cleaved and of blastocysts were significantly boosted (P<0.01) as sperm concentration at fertilization was elevated from 5${\times}$10$^4$ to 1${\times}$10$^{5}$ sperm/$m\ell$, but were not different between 1${\times}$10$^{5}$ and 5${\times}$10$^{5}$ sperm/$m\ell$.
Ischemic preconditioning is known to have protective effect on myocardial function at prolonged ischemic insult but the mechanism of the effect is not clearly known. The effect of the preconditioning on the global ischemia using cardioplegic solution is not well known. To evaluate the effect of global myocardial preconditioning on the functional recovery after cardioplegic arrest and two hours of hypothermic storage, we used the isolated rat heart and two hours cardioplegic arrest time at $0^{\circ}C$. In the experimental group(n=10), after baseline functional data was obtained, ischemic preconditioning was induced with 1 min of global normothermic ischemia for three times before the arrest period. In the control group(n=10), hearts underwent no ischemic precondi- tioning. After 2 hrs of cardioplegic arrest and storage in the $0^{\circ}C$ cardioplegic solution reperfusion was done and hemodynamic data were collected at post-reperfusion 20 min. Heart with ischemic preconditioning showed improved functional recovery at post reperfusion 20 min in peak developed pressure and dP/dT. In percent change of the peak pressure, preconditioning group showed 93.20$\pm$15.7% recovery rate compared to baseline data, and control group showed 67.3$\pm$15.6% recovery rate. In percent change of the dP/dT, control group showed 54.7$\pm$18.2% recovery rate and preconditioning group showed 78.1$\pm$15.1% recovery rate. Percent changes in heart rate and coronary flow showed no significant difference between two groups and there was no significant differences in amount of cardioplegic delivery between groups. Our data suggest ischemic preconditioning may have protective effect on recovery state after cardioplegic arrest and 2 hr ischemic storage of isolated rat heart and its mechanism is not related to the amount of the cardioplegic delivery amount.
Purpose : The acute respiratory distress syndrome(ARDS), an acute form of severe alveolar-capillary injury evolving after a direct or indirect lung insult is thought to be a common cause of respiratory failure though not many clinical studies on the subject have been made yet. Methods : Between January 1992 and December 2001, we conducted a retrospective study on 33 children who fulfilled the definition of the ARDS recommended by the American-European Consensus Conference in 1994. Results : A total of 33 patients(20 boys and 13 girls) were selected. Their age ranged from 4 months to 12 years with seven children younger than 1 year. The overall mortality rate was 78.8% and no significant difference was noted based on age or sex. Concerning seasonal variation, incidence of the ARDS increased in spring, especially in May(21.2%). Pneumonia(66.7%) was found to be the most common risk factor of the ARDS followed by sepsis(24.2%) and aspiration(3.0%). In immune compromised children(six cases), including a recipient of bone marrow transplantation, the mortality rate was 100%. Compared with children with multiple organ failure recording a 83.3% mortality rate, those with isolated respiratory failure, showed a lower mortality rate of 33.3%, although stastistically insignificant. Between survivor and non-survivor groups, significant differences were shown in hematocrit, $PaO_2$, $PaCO_2$, PEEP, and $PaO_2/FiO_2$ on the seventh day after the onset of the ARDS. Conclusion : According to our study, respiratory failure proved to have a great effect on mortality rate in the ARDS. More aggressive intervention and further studies on this subject should be done to improve the survival rate.
Kim, Dong-Su;Kim, Il-Kyu;Jang, Keum-Soo;Park, Tae-Hwan;Kim, Kyu-Nam;Son, Choong-Yul
Journal of the Korean Association of Oral and Maxillofacial Surgeons
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v.34
no.2
/
pp.166-179
/
2008
Excessive concentration of stress which is occurred in occlusion around the implant in case of the implant supported fixed partial denture has been known to be the main cause of the crestal bone destruction. Therefore, it is essential to evaluate the stress analysis on supporting tissue to get higher success rates of implant. The purpose of this study was to evaluate the effects of stress distribution and deformation in 3 different types of three-unit fixed partial denture sup-ported by two implants, using a three dimensional finite element analysis in a three dimensional model of a whole mandible. A mechanical model of an edentulous mandible was generated from 3D scan, assuming two implants were placed in the left premolars area. According to the position of pontic, the experiments groups were divided into three types. Type I had a pontic in the middle position between two implants, type II in the anterior posi-tion, and type III in the posterior position. A 100-N axial load was applied to sites such as the central fossa of anterior and posterior implant abutment, central fossa of pontic, the connector of pontic or the connector between two implants, the mandibular boundary conditions were modeled considering the real geometry of its four-masticatory muscular supporting system. The results obtained from this study were as follows; 1. The mandible deformed in a way that the condyles converged medially in all types under muscular actions. In comparison with types, the deformations in the type II and type III were greater by 2-2.5 times than in the type I regardless of the loading location. 2. The values of von Mises stresses in cortical and cancellous bone were relatively stable in all types, but slightly increased as the loading position was changed more posteriorly. 3. In comparison with type I, the values of von Mises stress in the implant increased by 73% in Type II and by 77% in Type III when the load was applied anterior and posterior respectively, but when the load was applied to the middle, the values were similar in all types. 4. When the load was applied to the centric fossa of pontic, the values of von Mises stress were nearly $30{\sim}35%$ higher in the type III than type I or II in the cortical and cancellous bone. Also, in the implant, the values of von Mises stress of the type II or III were $160{\sim}170%$ higher than in the type I. 5. When the load was applied to the centric fossa of implant abutment, the values of von Mises stress in the cortical and cancellous bone were relatively $20{\sim}25%$ higher in the type III than in the other types, but in the implant they were 40-45% higher in the type I or II than in the type III. According to the results of this study, musculature modeling is important to the finite element analysis for stress distribution and deformation as the muscular action causes stress concentration. And the type I model is the most stable from a view of biomechanics. Type II is also a clinically accept-able design when the implant is stiff sufficiently and mandibular deformation is considered. Considering the high values of von Mises stress in the cortical bone, type III is not thought as an useful design.
Effects of dietary concentrate levels based on whole-crop barley silage (BS) on development and meat quality in growing Korean black goats were examined. A total of 36 male goats with similar age and BW were equally allocated to dietary four treatments in a randomized complete block design. Dietary treatments included controls(rice straw ad libitum plus 2.0% concentrate BW-1), and BS ad libitum plus three increasing levels of concentrate BW-1(2.0, 1.5 and 1.0%, respectively), expressed as BS 2.0, BS 1.5 and BS 1.0, respectively. The trial was lasted for 5 months from 14th of May to 12th of October, 2007. At the end of trial, all animals were slaughtered to analyze carcass characteristics and meat quality. Average daily gain was significantly(p<0.05) higher for BS 1.5 and BS 2.0 treatments than for controls. Daily feed intakes for controls(689.3 g) were significantly(p<0.05) higher than those of BS 1.0(585.5 g), but they were significantly(p<0.05) lower than BS 2.0(734.3 g). Carcass and meat percentages were significantly (p<0.05) higher for BS 2.0 and BS 1.5 than for control and BS 1.0 treatments. Shear force and water-holding capacity was greater(p<0.05) for BS 2.0 and BS 1.5 than other treatments. On the basis of feeding whole-crop barley silage to Korean black goats, as dietary concentrate inclusion levels increased, sensory results showed to have a better tendency. In conclusion, it was estimated that the optimal level of dietary concentrates might be 1.5% BW-1, when diets based whole-crop barley silage were fed to growing Korean black goats for their improvements of growth and meat quality.
Dollar spot, caused by Sclerotinia homoeocarpa, is the major disease in cool-season turfgrasses. Understanding the distribution of this pathogen in soil and thatch is important to developing disease control strategies. In this study, toothpicks were used to detect S. homoeocarpa in the turfgrass canopy, thatch, and soil at different distances from dollar spot infection centers. The effect of penetrant and contact fungicide applications with different water volumes on distribution of S. homoeocarpa was also investigated. S. homoeocarpa was detected in 100% of samples taken from the leaf canopy, 83.3% in thatch area, and 0% in the soil from within the infection center. S. homoeocarpa was isolated in 100% of samples taken from the edge of the infection center, but was only detected in 13% of the samples taken at 1.5 cm away from the infection center edge. S. homoeocarpa was isolated at a higher frequency in the propiconazole treated plots than those treated with chlorothalonil and was not detected in leaf canopy samples when either fungicides was applied with 6.78 L of water. In conclusion, the toothpick-aided detection technique has improved our understanding of S. homoeocarpa epidemiology and could be used as a diagnostic tool to detect for fungicide resistance on golf courses.
Surface image velocimetry was introduced as an efficient and sage alternative to conventional river flow measurement methods during floods. The conventional surface image velocimetry uses a pair of images to estimate velocity fields using cross-correlation analysis. This method is appropriate to analyzing images taken with a short time interval. It, however, has some drawbacks; it takes a while to analyze images for the verage velocity of long time intervals and is prone to include errors or uncertainties due to flow characteristics and/or image taking conditions. Methods using spatio-temporal images, called STIV, were developed to overcome the drawbacks of conventional surface image velocimetry. The grayscale-gradient tensor method, one of various STIVs, has shown to be effectively reducing the analysis time and is fairly insusceptible to any measurement noise. It, unfortunately, can only be applied to the main flow direction. This means that it can not measure any two-dimensional flow field, e.g. flow in the vicinity of river structures and flow around river bends. The present study aimed to develop a new method of analyzing spatio-temporal images in two-dimension using cross-correlation analysis. Unlike the conventional STIV, the developed method can be used to measure two-dimensional flow substantially. The method also has very high spatial resolution and reduces the analysis time. A verification test using artificial images with lid-driven cavity flow showed that the maximum error of the method is less than 10 % and the average error is less than 5 %. This means that the developed scheme seems to be fairly accurate, even for two-dimensional flow.
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