• Title/Summary/Keyword: 동물사체

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Application of chemical treatment for cattle and chicken carcasses for the control of livestock infectious diseases (가축 전염병 발생에 따른 소와 닭 사체의 화학적 처리 방법의 적용)

  • Lee, Taek Geun;Oh, Yeonsu;Ko, Young-Seung;Bae, Da-Yun;Tark, Dong-Seob;Rim, Chaekwang;Cho, Ho-Seong
    • Korean Journal of Veterinary Service
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    • v.45 no.2
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    • pp.117-124
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    • 2022
  • In the event of an outbreak of a livestock epidemic, it has been considered that the existing burial-centered carcass disposal method should be improved ecofriendly for prevention of leachate and odors from burial basically in regard of pathogen inactivation. Therefore, the aim of this study is whether it was possible to treat the carcass of cattle and chickens using the chemical carcass treatment method. It was conducted to establish detailed treatment standards for the chemical treatment method of cattle and chicken carcasses based on the results of the proof of the absence of infectious diseases in cattle chickens. After inoculating cattle carcass with 10 pathogens (foot and mouth disease virus, bovine viral diarrhea virus, Mycobacterium bovis, Mycobacterium avium subsp. Paratuberculosis, Brucella abortus, Bacillus anthracis, Clostridium chauvoei, Clostridium perfringens, Escherichia coli, and Salmonella Typhimurium) and chicken carcasses with low pathogenic avian influenza virus, Clostridium perfringens type C, E. coli and Salmonella Typhimurium, these were treated at 90℃ for 5 hours in a potassium hydroxide liquid solution corresponding to 15% of the body weight. This method liquefies all cadaveric components and inactivates all inoculated pathogens by PCR and culture. Based on these results, it was possible to prove that chemical treatment of cattle and chicken carcasses is effective in killing pathogens and is a safe method without the risk of disease transmission. The chemical treatment method of livestock carcasses can be suggested as an alternative to the current domestic burial-centered livestock carcass treatment method, preventing environmental pollution, and contributing to public health.

The Efficiency and Improvement of the Highway Wild-Life Fences for Decrease of Mammals Road-kill - In Case of Manjong~Hongchun Section on Jungang Highway - (포유류 로드킬 저감을 위한 고속도로 유도울타리 효율성 및 개선방안 연구 - 중앙고속도로 만종~홍천 구간을 사례로 -)

  • Song, Jeong-Seok;Lee, Kyong-Jae;Ki, Kyong-Seok;Jun, Ik-Yo
    • Korean Journal of Environment and Ecology
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    • v.25 no.5
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    • pp.649-657
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    • 2011
  • This study had targeted the Manjong Junction~Hongchun interchange section of Jungang highway in order to analyze the efficiency and improvement of the highway wild-life fences. Being analyzed wild-life fence as an effective facility for the prevention of road-kill, it had founded that the wild animals did not jump over even the two layer guardrail, concrete barrier and noise barrier, fence for rock fall. For the section with high road-kill decrease effect after installing the wild-life fence, the effect was higher at wild-life fence with the height of 1.0m where the door had not been installed and the well connected points of road facility than the area with fence height of 1.5m. The road-kill decrease effect was also high at the well managed areas even if the door has not been installed. Also, road-kill had occurred by concentrating around the end of wild-life fence after installing the fence, Moreover road-kill had also occurred around the cutting section. The door of wild-life fence had higher amount of road-kill occurrence as the installation interval was closer. it was analyzed that the door management has a lot of effect on road-kill decrease. The fence for rock fall, two layer guardrail and concrete barrier having the effect of wild-life fence installed on the road would have to be installed by connecting with wild-life fences through proper facility improvement. Although the door should not be installed if possible, it should be installed as automatic door or gravity door to prevent the door from leaving the door open. An escape route has to be formed for the prompt escape away from the road for the animals entered through the ending section of the fence. The eco-corridor has to be made by restoring the soil layer of dual purpose eco-corridor forming a planting area. Also, the dead body after the accident has to be disposed to the outer section of the road immediately in order to prevent the secondary road-kill by the predator from the road-kill. The fence has to be installed as 500m or longer in both ways, in other words 1,000m or longer, from the targeted spot of minimum accident prevention while connecting up to the bridge or box culvert, etc that are next road facilities if possible to guide wild animals safely to the eco-corridor.

Study on the Activation Plan for Utilization of Agri-food by-products as Raw Materials for TMR (TMR 원료로 이용하는 농식품 부산물 사료 이용 활성화 방안에 관한 연구)

  • Chung, Sung Heon;Park, Hyun Woo;Kwon, Byung Yeon;Gu, Gyo Yeong;Bang, Seo Yeon;Park, Kyung Soo
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.34 no.4
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    • pp.296-306
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    • 2014
  • This study was conducted to survey and analyze the quantity of various organic wastes and to vitalize the utilization of agri-food by-products as raw materials for Total mixed ration (TMR), to improve feed cost savings and the quality of animal products. On-the-spot obstacles for animal farmers, along with legal and institutional alternatives are presented. The results are as follows. First, organic wastes in Korea are managed by the Allbaro system created in the Wastes Control Act, which processes 10,488 tons of cooking oil waste, 832,493 tons of animal and plant residues, 5,740 tons of animal carcasses, 1,171,892 tons of animal residues, and 2,172,415 tons of plant residues including 12,905 tons of rice hull and bran, for a total of 4,205,931 tons. Raw materials for TMR, namely rice hulls and bran as well as plant residues, accounted for 51.7% of the total national organic waste. The top 10 municipalities process 76~100% of all organic wastes and a supply management system is needed for the waste. Second, the 10 major agri-food by-products used as raw materials for TMR are bean curd by-product, rice bran, oil-cake, brewers dried grain, Distiller's Dried Grains with Solubles (DDGS), barley bran, soy sauce by-product, citrus fruit by-product, mushroom by-product and other food by-product (bread, noodles, snacks, etc.). Third, the biggest difficulties in using agri-food by-products are legal obstacles. Because agri-food by-products are regulated as industrial wastes by the Waste Control Act, animal farmers that wish to use them have legal reporting obligations including the installation of recycling facilities. To enable the use of agri-food by-products as raw materials for TMR, waste management system improvements such as 'the end of waste status' and the establishment of more than 10 public distribution centers nationwide are deemed essential.

A Study of Potential of Diet Analysis in the Korean Water Deer(Hydropotes inermis argyropus) using Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis(PCR-DGGE) (고라니의 식이물 분석에 있어 Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis(PCR-DGGE)의 이용 가능성 연구)

  • Park, Ji-Eun;Kim, Baek-Jun;Lee, Sang-Don
    • Korean Journal of Environment and Ecology
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    • v.24 no.3
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    • pp.318-324
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    • 2010
  • The aim of this study is to examine feeding habits of the Korean water deer(Hydropotes inermis argyropus) from its rumen contents using a PCR-DGGE method. For this study, rumen contents were collected from water deer causalities by natural death or road-kill in two different sites(Cheorwon, Gangwon province and the Eastern part of Jeonnam province). DNA was extracted from rumen contents of a total of 44 individuals. Two primers, rbcLZ1aF(GC) and rbcL19bR, were used for PCR amplifications of ribulose-1,5-bisphosphate carboxylase large subunit (rbcL) gene. Among 44 samples, twenty-nine samples were successfully amplified by PCRs. The 29 PCR products of partial rbcL gene were applied for PCR-DGGE. Totally, six families of plants were detected from the diet analyses. Five families of plants were found in Cheorwon, Gangwon province, but only three families of plants were found in the Eastern part of Jeonnam province. The PCR-DGGE method will provide us with a potential tool to study feeding habits of ungulates including water deer, even though our results failed to identify the prey plants at the level of species.

Effect of Mycelium of Cordyceps militaris on Growth, Lipid Metabolism and Protein Levels in Male Rats (동충하초 균사체가 흰쥐의 성장률, 지질대사 및 단백질 농도에 미치는 영향)

  • Koh, Jin-Bog
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.31 no.4
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    • pp.685-690
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    • 2002
  • The effects of mycelium of Cordyceps militaris on the growth, the lipid metabolism, the serum protein levels and the enzyme activities in male rats were studied. Sprague-Dawley rats were given four different types of diets for a succeeding period of five weeks: either a control diet, a control diet supplemented with 2%, 3% or 4% mycelium of Cordyceps militaris (CM) powder. The body weight gain, hepatic weight, feed efficiency ratio and the feed intake of the rats given diets with 2%, 3% or 4% CM were similar to those in rats given the control diet. The concentrations of hepatic total lipid and triglyceride of rats fed the 3% or 4% CM diets were significantly lower than those of rats fed the control diet. But the concentrations of hepatic total cholesterol and phospholipid of rats fed the all CM diets were similar to those of rats fed the control diet. The concentrations of total lipid, total cholesterol, triglyceride phospholipid, and the atherogenic index, and the activities of glutamic oxaloacetic transaminase and lactic dehydrogenase in serum of rats fed the all CM diets were significantly lower than those of rats fed the control diet. No differences were noted in the concentrations of HDL-cholesterol, LDL-cholesterol, total protein, albumin and creatinine, and the activities of glutamic pyruvic transaminase, ${\gamma}$ -glutamyltranspeptidase and alkaline phosphatase in the serum among the rats with on all the experimental diets. These results showed that the all CM diets feeding decreased the total cholesterol, the triglyceride, the phopholipid, and the atherogenic index in serum of rats.

The Gut Content Analysis of Polypedilum scalaenum in the Large-scale Weirs of 4 Major River Ecosystems (4대강 보에 서식하는 삼지창무늬깔따구(Polypedilum scalaenum) 위 내용물 분석)

  • Na, Young-Kwon;Jo, Hyunbin;Park, Jae-Won;Chang, Kwang-Hyeon;Kwak, Ihn-Sil
    • Korean Journal of Ecology and Environment
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    • v.53 no.1
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    • pp.55-62
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    • 2020
  • Chironomidae widely utilize as a biological indicator that has various types of feeding pattern such as omnivorous, herbivorous and carnivorous. Polypedilum scalaenum is known as omnivorous, it plays an important role in the food chain of freshwater ecosystems in Korea. Nevertheless, detailed information about diet items of P. scalaenum is still lacking. The purpose of this study is to identify the gut and intestine contents of items on P. scalaenum inhabiting the large-scale weirs of the four major river ecosystems(Ipoh Weir, Sejong Weir, Juksan Weir, Gangjeong-Goryeong Weir, and Dalseong Weir). Phytoplankton was dominant diet items among the study sites. However, zooplankton (i.e. appendages, setae) only found in Ipoh Weir. The phytoplankton species composition in the study sites was correspond to the diet items in the gut and intestine contents of P. scalaenum. In summary, analysis of P. scalaenum gut and intestine contents in this study was able to identify the feeding characteristics of omnivorous Chironomidae, and in particular, it was possible to study the species composition of basic producers in the surrounding aquatic environment by analyzing the contents of the digestive tracts.

Tree Growth and Ecosystem Conditions of Docheon Forest (Natural Monument No. 514) in Docheon-ri, Yeongdeok - Focusing on Plant Ecosystems - (천연기념물 제514호 영덕 도천리 도천숲의 생태계 현황 및 수목 생육 특성 - 식물생태계 현황을 중심으로 -)

  • Kim, Dong Wook;Hur, Bog Su;Lee, Seung Joo;Kim, Hyo Jeong
    • Korean Journal of Heritage: History & Science
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    • v.44 no.1
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    • pp.122-137
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    • 2011
  • The purpose of this study was to present the characteristics of tree growth and the status of the ecosystem in Yeongdeok Docheon-ri Docheon forest, which was designated as Natural Monument No. 514. Results showed that most of the soil properties were generally within desirable levels except for some properties. The forest was composed of 503 trees. Major trees included Zelkova serrata, Celtis sinensis, Cornus walteri, Diospyros lotus, Hemiptelea davidii, and Evodia daniellii. In particular, trees such as Zelkova serrata, Celtis sinensis, and Cornus walteri showed D.B.H ranging from 10cm to 70cm. These trees appeared to be major tree species since the beginning of Docheon forest's existence. The wild birds observed from the site were from 18 families, 20 species, and 95 individuals. Also were found 1 mammal species, 1 reptile species, and 1 amphibian species. A dead Aix galericulata (designated Natural Monument No. 327) was found in the forest. The annual tree diameter growth of the sample trees was 2.3mm per year on average. The average age of the overstory trees was approximately 101 years, and the oldest tree was estimated to be 300~400 years old.

A Thoracic Spine Segmentation Technique for Automatic Extraction of VHS and Cobb Angle from X-ray Images (X-ray 영상에서 VHS와 콥 각도 자동 추출을 위한 흉추 분할 기법)

  • Ye-Eun, Lee;Seung-Hwa, Han;Dong-Gyu, Lee;Ho-Joon, Kim
    • KIPS Transactions on Software and Data Engineering
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    • v.12 no.1
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    • pp.51-58
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    • 2023
  • In this paper, we propose an organ segmentation technique for the automatic extraction of medical diagnostic indicators from X-ray images. In order to calculate diagnostic indicators of heart disease and spinal disease such as VHS(vertebral heart scale) and Cobb angle, it is necessary to accurately segment the thoracic spine, carina, and heart in a chest X-ray image. A deep neural network model in which the high-resolution representation of the image for each layer and the structure converted into a low-resolution feature map are connected in parallel was adopted. This structure enables the relative position information in the image to be effectively reflected in the segmentation process. It is shown that learning performance can be improved by combining the OCR module, in which pixel information and object information are mutually interacted in a multi-step process, and the channel attention module, which allows each channel of the network to be reflected as different weight values. In addition, a method of augmenting learning data is presented in order to provide robust performance against changes in the position, shape, and size of the subject in the X-ray image. The effectiveness of the proposed theory was evaluated through an experiment using 145 human chest X-ray images and 118 animal X-ray images.

Effects of Fermented Rice Wine by Using Mycelium of Phellinus linteus on the Expression of Inflammation-Related Proteins in Human Hepatoma Cells and Rat Liver (상황버섯 균사체를 이용한 발효주가 인체간암세포와 흰쥐 간의 염증관련 단백질 발현에 미치는 영향)

  • Ahn Seung-Min;Lee Jun-Hyuk;Choi Yung-Hyun;Lee Yong-Tae;Chung Kyung-Tae;Jeong Young-Kee;Jo Un-Bock;Choi Byung-Tae
    • Journal of Life Science
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    • v.16 no.1
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    • pp.101-107
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    • 2006
  • We have recently discovered that mycelium of Phellinus linteus, popular medical mushrooms in Korea, possess alcohol dehydrogenase and produce alcohol. In the present study, it was examined that the effect of fermented rice wine made by using mycelium of P. linteus (FLMP) on the expression of in-flammation-related proteins in both $HepG_2$ cells and rats. To examine the effect of FLMP on the morphology and expression of inflammatory proteins in $HepG_2$ cells, the cells were incubated with ethanol, and FLMP for 24 hours, and then analyzed by microscopic observation and Western blot and reverse transcription polymerase chain reaction (RT-PCR). While ethanol induced the morphological change accompanied with cell debris formation and scattering on $HepG_2$ cells, FLMP had no effect. The results of Western blot and RT-PCR analyses showed that the level of inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-1 and COX-2 was induced by ethanol, however, FLMP inhibited the expression of these proteins and its mRNAs. In the animal model, the value of flutamate oxaloacetate transaminase and glutamate pyruvate transaminase was significantly increased by administration with ethanol. But the group administrated with FLMP showed lower levels on the changes of these markers compared with ethanol-administrated group. Besides, the results of Western blot and RT-PCR analyses showed that the expression of inflammatory proteins such as iNOS, COX-1 and COX-2 was not affected by FLMP administration in rat liver. About histopathological and immunohistochemical observations, inflammatory loci were markedly decreased in the FLMP-administrated rat compared to ethanol-administrated rats and showed weaker COX-2 and iNOS jmmunoreactions. These results suggested that FLMP showed slight changes on the inflammatory proteins expression compared to ethanol and FLMP may be used as a functional alcoholic beverage.

Confucians Funeral Rituals during the mid-Joseon Dynasty Lee Mun Geon'Mourning beside His Mother's Grave (이문건 시묘살이를 통해 본 조선중기 유자(儒者)의 상례(喪禮) 고찰)

  • Cho, Eun-suk
    • Journal of Korean Classical Literature and Education
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    • no.33
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    • pp.153-184
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    • 2016
  • This study investigates the funeral rituals practiced by the Joseon Dynasty as recorded about Lee Mun Geon (1494~1567, a.k.a Mukjae), who mourned by the grave of his deceased mother, Ms. Shin (1463~1535), a woman whose family's origin was Goryeon. The study focuse on the rituals performed by Lee after his mother's death, his participation in the funeral, and his mourning specifically as an individual who has lost his parent. Reviewing Lee's mourning life beside the grave, the contents of diary belonging to a nobleman in the middle of Joseon Dynasty were studied aimsing to find out the meaning of rituals, the overall recognition accorded to death, and the filial duties that were carried out by the noblemen of the time. Although noblemen in the middle of Joseon Dynasty ceaselessly attempted to change the observance of funeral rituals through legislation, it was difficult to change the mindset of the people, who fllowed the deep-rooted traditions of long history. It must be acknowledged that the Joseon Dynasty had a different cultural background than that of China. There was a fundamental problem when they tried to adapt The Family Rituals of Zhu Xi, followed by the Chinese, to the Joseon society. Although The Family Rituals of Zhu Xi emphasized ancestral rites focusing on enshrining mortuary tablets and the importance of establishing the family shrine hundred times, noblemen in the mid-Joseon Dynasty period cared for their parents in the grave by mourning for them than by following such practice. The solemn memorial service held in front of the grave, and the annual ritual service on the death anniversary were far more important to the noblemen in the mid-Joseon Dynasty. Amid such contradictions, the noblemen accepted and performed the mourning rituals beside the grave of their parent. Human beings across the ages have always dwelt upon thoughts of the afterlife. Most people believe that they attain a state after the death of their physicalbody. If humans did not have such thoughts, they would not be bothered if death occurs on being hit by a car on the street. Thus, human beings often think of the ritual services related to death, although in different forms. Therefore, mourning by the grave of their parent held great significance among the noblemen of the Joseon Dynasty as a sign of their filial piety.