Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effect of Mycelium of Cordyceps militaris on Growth, Lipid Metabolism and Protein Levels in Male Rats

동충하초 균사체가 흰쥐의 성장률, 지질대사 및 단백질 농도에 미치는 영향

  • 고진복 (신라대학교 생명과학과)
  • Published : 2002.08.01
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Abstract

The effects of mycelium of Cordyceps militaris on the growth, the lipid metabolism, the serum protein levels and the enzyme activities in male rats were studied. Sprague-Dawley rats were given four different types of diets for a succeeding period of five weeks: either a control diet, a control diet supplemented with 2%, 3% or 4% mycelium of Cordyceps militaris (CM) powder. The body weight gain, hepatic weight, feed efficiency ratio and the feed intake of the rats given diets with 2%, 3% or 4% CM were similar to those in rats given the control diet. The concentrations of hepatic total lipid and triglyceride of rats fed the 3% or 4% CM diets were significantly lower than those of rats fed the control diet. But the concentrations of hepatic total cholesterol and phospholipid of rats fed the all CM diets were similar to those of rats fed the control diet. The concentrations of total lipid, total cholesterol, triglyceride phospholipid, and the atherogenic index, and the activities of glutamic oxaloacetic transaminase and lactic dehydrogenase in serum of rats fed the all CM diets were significantly lower than those of rats fed the control diet. No differences were noted in the concentrations of HDL-cholesterol, LDL-cholesterol, total protein, albumin and creatinine, and the activities of glutamic pyruvic transaminase, ${\gamma}$ -glutamyltranspeptidase and alkaline phosphatase in the serum among the rats with on all the experimental diets. These results showed that the all CM diets feeding decreased the total cholesterol, the triglyceride, the phopholipid, and the atherogenic index in serum of rats.

인공 재배한 동충하초 밀리타리스의 균사체가 성장기 흰쥐의 영양 생리에 미치는 효과를 평가하고자 생후 5주령된 흰쥐를 대상으로 실험군은 대조식이군, 대조식이에 동충하초 균사체 분말을 각각 2%, 3% 및 4% 첨가한 식이군(균사체군) 등 4군으로 나누고, 각 실험식이로 5주간 사육한 후 여러 영양생리적 지표를 측정하였다. 실험동물의 성장률, 식이효율, 식이섭취량 및 각 장기의 무게는 2%, 3% 및 4% 균사체군은 대조군과 비슷하여 균사체 섭취에 따른 영향은 나타나지 않았다. 간의 콜레스테롤 및 인지질 농도는 각 수준별 균사체군은 대조군과 비슷하였으나, 간의 총 지질 및 중성지질 농도는 3%와 4% 균사체군은 대조군보다 유의하게 감소하였다. 혈청의 총 지질, 총 콜레스테롤, 중성지질, 인지질 농도 및 동맥경화지수는 대조군에 비하여 각 수준별 균사체군이 유의하게 감소하여 동충하초균사체가 혈청의 지질 농도를 낮추는 효과가 나타났다. 혈청의 HDL-졸레스테롤과 LDL-콜레스테롤 농도 및 총 콜레스테롤에 대한 HDL-콜레스테롤 비율은 대조군과 각 수준별 균사체군이 비슷하였다. 혈청의 총 단백질, 알부민, creatinine및 혈색소 농도와 GPT, ${\gamma}$-GTP 및 ALP 등의 효소활성은 각 수준별 균사체군과 대조군이 비슷하여 동충하초 섭취에 따른 영향은 나타나지 않았다. 혈청의 GOT및 LDH활성은 대조군에 비하여 각 수준별 균사체군이 유의하게 감소하였고, 혈청의 요소 농도는 대조군에 비하여 각 수준별 균사체군이 유의하게 증가하였다.

Keywords

References

  1. Samson RA, Evans HC, Latge JP. 1988. Atlas of entomopathogenic fungi. Springer, Heidelbeerg. p 189-196.
  2. Kobayashi Y, Shimizu D. 1983. Iconography of vegetable wasps and plant worms. Hoikusha Pub Co Ltd, Osaka. p 280-285.
  3. Sung JM. 1996. Cordyceps of Korea. Kyo-Hak Publishing Co, Seoul. p 13-18.
  4. Jianzhe Y, Xiaoloan M, Qiming M, Yichen Z, Huaan W. 1989. Icons of Medicinal Fungi from China. Science Press, China. p 575-585.
  5. Shimizu D. 1994. Color iconography of vegetable wasps and plant worms. Seibundo Shinkosha, Japan. p 381-387.
  6. Zhu JS, Halpern GM, Jones K. 1998. The scientific rediscovery of an ancient Chinese herbal medicine, Cordyceps sinensis Part I. J Altern Complement Med 4: 289-303. https://doi.org/10.1089/acm.1998.4.3-289
  7. Zhu JS, Halpern GM, Jones K. 1998. The scientific rediscovery of an ancient Chinese herbal regmen, Cordyceps sinensis Part II. J Altern Complement Med 4: 2429-2457.
  8. Kuo YC, Lin CY, Tasi WJ, Wu CL, Chen CF, Shiao MS. 1994. Growth inhibitors against tumor cells in Cordyceps sinensis other than cordycepin and polysaccharides. Cancer Invest 12: 611-615. https://doi.org/10.3109/07357909409023046
  9. Nan JX, Park EJ, Yang BK, Song CH, Ko G, Sohn DH. 2001. Antifibrotic effect of extracellular submerged mycelial cultures of Cordyceps militaris on liver fibrosis induced by bile duct ligation and scission in rats. Archives of Pharmacal Research 24: 327-332. https://doi.org/10.1007/BF02975101
  10. Liu J, Yang S, Yang X, Chen Z, Li J. 1997. Anticarcinogenic effect and hormonal effect of Cordyceps militaris Link. China Journal of Chinese Materia Medica 22: 111-113.
  11. Shen Q, Chen S. 2001. Effect of Cordyceps militaris on the damage of rats induced by n-hexane. Zhong Yao Cai 24: 112-116.
  12. Chang HL, Chao GR, Chen CC, Mau JL. 2001. Non-volatile taste components of Agaricus blazei, Antrodia camphorata and Cordyceps militaris mycelia. Food Chemistry 74: 203-207. https://doi.org/10.1016/S0308-8146(01)00127-3
  13. Koh JB, Choi MA. 2001. Effect of Cordyceps militaris on lipid metabolism in rats fed cholesterol diet. Korean J Nutrition 34: 265-270.
  14. Koh JB. 2001. Effect of fruiting body of Cordyceps militaris on growth, lipid and protein metabolism and enzyme activities in male rats. Korean J Nutrition 34: 741-747.
  15. Reeves PG, Nielsen FH, Fahey GC. 1993. AIN-93 purified diets for laboratory rodents final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J Nutr 123: 1939-1951. https://doi.org/10.1093/jn/123.11.1939
  16. Davidson I, Henry JB. 1996. Todd-Sanford Clinical Diagnosis by Laboratory Methods. 13th ed. WB Saunders Co, Philadelphia. p 73-76.
  17. Friedewald WT, Levy RI, Fedreison DS. 1979. Estimation of concentration of low density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 18: 499-508.
  18. Haglund O, Loustarinen R, Wallin R, Wibell I, Saldeen T. 1991. The effect of fish oil on triglycerides, cholesterol, fibrinogen and malondialdehyde in humans supplemented with vitamin. Eur J Nutr 121: 165-172. https://doi.org/10.1093/jn/121.2.165
  19. Folch J, Lees M, Stanley GSH. 1957. A simple method for the isolation and purification of total lipid from animal tissues. J Biol Chem 226: 497-509.
  20. Frings CS, Dunn RT. 1970. A colorimetric method for determination of total serum lipid based on the sulfophosphovanillin reaction. Am J Clin Path 53: 89-91. https://doi.org/10.1093/ajcp/53.1.89
  21. Sugiyama K, Saeki S, Ishiguro Y. 1992. Hypercholesterolemic activity of ningyotake (Poyporus confluens) mushroom in rats. J Jpn Soc Nutr Food Sci 45: 265-270. https://doi.org/10.4327/jsnfs.45.265
  22. Bobek P, Ginter E, Kuniak L, Babala J, Jurcovicova M, Ozdin L, Cerven J. 1991. Effect of mushroom Pleurotus ostreatus and isolated fungal polysaccharide on serum and liver lipid in Syrian hamsters with hyperlipoproteinemia. Nutrion 7: 105-109.
  23. Kim GJ, Kim HS, Kim SH, Kim HS, Choi WJ, Chnng SY. 1994. Effects of the feeding mixture of murshrooms and vegetable oils on the lipid component and fatty acid composition on liver in rats. J Korean Soc Food Sci Nutr 23: 736-742.
  24. Choi MY, Lim SS, Chung TY. 2000. The effects of hot water soluble polysaccharides from Lentinus edodes on lipid metabolism in the rats fed butter yellow. J Korean Soc Food Sci Nutr 29: 294-299.
  25. Kwon SH, Woo HJ, Han DS, Kim MK. 2001. Effect of dried powders and water extracts of Paecilomyces tenuipes and Cordyceps militaris on lipid metabolism, antidxidative capacity and immune status in rats. Korean J Nutrition 34: 271-284.
  26. Kiho T, Yamane A, Hui J, Usui S, Ukai S. 1996. Polysaccharides in fungi XXXVI. Hypoglycemic activity of polysaccharide (CS-F30) from the cultural mycelium of Cordyceps sinensis and its effect on glucose metabolism in mouse liver. Biol Pharm Bull 19: 294-296. https://doi.org/10.1248/bpb.19.294
  27. Lin Y. 1999. Phamacological effect on Cordyceps sinensis. paper presented at 1st international symposium on Cordyceps. The Korean Society Sericultural Science and the Korean Society of Life Science, Seoul, Korea. p 35-44.
  28. Kabir Y, Kimura S, Tamura T. 1988. Dietary effect of Ganoderma lucidum mushroom on blood pressure and lipid levels in spontaneously hypertensive rats. J Nutr Sci Vitaminol 34: 433-438. https://doi.org/10.3177/jnsv.34.433
  29. Chang R. 1996. Functional properties of edible mushroom. Nutr Rev 54: S91-S93. https://doi.org/10.1111/j.1753-4887.1996.tb03825.x
  30. Sugiyama K, Akachi T, Yamakawa A. 1995. Hypocholesterolemic action of eritadenine is mediated by a modification of hepatic phospholipid metabolism in rats. J Nutr 125: 2134-2144. https://doi.org/10.1093/jn/125.8.2134
  31. Indira M, Kurup PA. 1989. Effects of neutral detergent fiber from blakgram (Phaseolus mungo) in rats rabbits. J Nutr 119: 1246-1251. https://doi.org/10.1093/jn/119.9.1246
  32. Kato T, Takemoto K, Ketayama H, Kuwabara Y. 1984. Effects of Spirulina (Spirulina platensis) on dietary hypercholesterolemia in rats. J Jpn Soc Nutr Food Sci 37: 323-332 https://doi.org/10.4327/jsnfs.37.323
  33. Yeon SH. 2000. The chemical compositions and biological activities of Cordyceps militaris. MS Thesis. Seoul National University.
  34. Kim HY, Song SW, Ha CS, Han SS. 1993. Effects of the population density on growth and various physiological values of Sprague-Dawley rats. Korean J Lab Ani Sci 9: 71-78.
  35. Kang BH, Son HY, Ha CS, Lee HS, Song SW. 1995. Reference value of hematology and serum chemistry in Ktc: Sprague-Dawley rats. Korean J Lab Ani Sci 11: 141-145.

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