• Korean Journal of Food Science and Technology
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• v.39 no.6
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• pp.688-693
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• 2007

The hepatoprotective effects of water extracts composed of Adenophora triphylla var japonica Hara (ATJH) on acetaminophen (APAP)-induced hepatotoxicity were investigated in vivo and in vivo. The effects of ATJH on liver toxicity induced by APAP were assessed by blood biochemical and histopathological analyses. APAP treatment (350 mg/kg) caused severe liver injury in mice as indicated by their significantly elevated plasma aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels. Pretreatment with ATJH for 3 or 7 days attenuated the increases in ALT and AST when challenged with APAP. The reductions in viability caused by high dose of APAP (450 mg/kg) in vivo were reversed by pretreatment with ATJH. These protective effects of ATJH against APAP-induced toxicity were consistent with the results from the histopathological examinations. We next examined the effects of ATJH on the gene expression of glutathione S-transferases (GSTs) that detoxify the metabolic intermediates of APAP in H4IIE cells. The hepatic GST protein levels [$\alpha$ class (GSTA2, GSTA3/5)] were significantly elevated in a dose-dependent manner by ATJH treatment. In summary, ATJH is effective at protecting against APAP-induced hepatotoxicity by GST induction, implying that ATJH should be considered a potential chemopreventive agent.

• Journal of Digital Convergence
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• v.19 no.1
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• pp.45-49
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• 2021

Recently, as more attention has been paid to the issues of environmental pollution and animal tests, vegan beauty has become a new trend as an large axis of consumption. Therefore, this study aimed to speculate on the vegan beauty and investigate its corelation with beauty industry through a survey, giving a focus on perception, characteristics, the current status and supplementation of vegan beauty. The results of the survey were: Only 31.3% of the subjects were aware of vegan beauty; In respect to characteristics, the mean value of advantage was 3.08, which was higher than that of disadvantages, 3.04. As for the current status, the rate of purchase intention was high, 81.7% and the rate of outlook was very high, 87.3%. Although vegan beauty has not been popular yet, if price is adjusted, products are diversified and generalized, and performance is improved, it is suggested that it will present positive outlook to future development of beauty industry.

• Korean Journal of Acupuncture
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• v.25 no.1
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• pp.155-164
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• 2008

목 적 : 본 연구의 목적은 양릉천 침 처치 시 C57BL/6 생쥐의 중뇌 흑질에 위치한 도파민성 신경세포 사멸 억제 효과를 조직화학 염색법을 이용하여 Tyrosine hydroxylase(TH)와 laminin의 발현으로 관찰하고자 한다. 실험방법 : 실험에 이용한 동물은 C57BL/6 생쥐로, 매일 25mg/kg의 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)를 5일간 주사하였고, 매일 MPTP 주사한 뒤 2시간 후에 양릉천에 침치료를 시행하였으며 MPTP 주사를 종료한 뒤 침치료는 7일동안 계속 시행하였다. 마지막 MPTP 주사 7일 후에 동물을 희생하여 뇌를 적출하고 고정하였다. 침효과를 확인하기 위해 Thyrosine hydroxylase(TH), laminin의 발현 변화 정도를 조직염색화학법으로 이용하여 확인하였다. 각 그룹간의 유의성 검증은 one-way ANOVA를 이용하였다. 결 과 : 도파민성 신경세포 선택적인 신경독소인 MPTP에 대한 양릉천 침처치에 의한 신경보호 효과를 도파민신경세포의 표지자인 TH 발현을 면역화학조직염색법으로 관찰하였다. 대조군에 비해 MPTP 처치 군의 신경세포 사멸이 유의적으로 감소하였고(P <0.05), MPTP + 침처치 군에서 증가되는 양상을 확인하였다 (P <0.05). 또한 도파민성 신경세포내에 존재하는 laminin의 발현정도 역시 대조군보다 MPTP 처치 군에서 유의적으로 감소하였고, MPTP + 침처치 군에서 증가되는 양상을 확인하였다 (P <0.05). 결 론 : MPTP에 의한 도파민성 신경세포 손상에 대한 양릉천 침처치의 신경보호 효과는 세포외 기질중의 하나인 laminin의 발현 정도를 조절하여 비롯되는 것으로 사료된다.

• KSBB Journal
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• v.21 no.1 s.96
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• pp.11-15
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• 2006

The biological activities of extracts from the fruit, stem, and leaf of Hovenia dulcis were examined. In the batch mode of operation, the fruit, stem, and leaf of Hovenia dulcis were extracted with hot water for 10 hr. The fruit extract of Hovenia dulcis gave the highest activity for decreasing alcohol concentration which was 138% of control. The equilibrium between bioactive compound in the fruit (size : 4 mm) and hot water solution was reached within 6 hr and the recovery was 95% by three-times extraction. The fruit extract of Hovenia dulcis showed significant alcohol decrease in blood and hepatoprotective activity against $CCl_4$-toxicity in rat. The fruit extract significantly inhibited the elevation of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH) levels.

• Korean Journal of Environment and Ecology
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• v.34 no.5
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• pp.436-445
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• 2020

The number of protected areas has been steadily increased in Korea to achieve Aichi Target 11, and there are studies on potential protected areas that required additional designation. However, there has been an insufficient assessment of the complementarity of protected areas to conserve biodiversity effectively. This study identified the potential habitat areas using the species distribution model for plant species from the 3rd National Ecosystem Survey and compared the plant species abundance in the existing protected area and the potential protected areas using the similarity indices, such as the Jaccard index, Sorenson index, and Bray-Curtis index. As a result, we found that the complementarity of the existing protected areas and most potential protected areas were low, leading to the preservation of similar plant species. Only the buffer zone for Korea National Arboretum had high complementarity and thus is important to conserve some species with the other protected areas. This study confirmed that it was necessary to select additional protected areas outside the existing or potential protected areas to protect plant species with a low inclusion ratio of potential habitats within the protected area. This study is significant because it identified the ecological representativeness of each protected area to examine if the individual protected area can conserve unique and various species and proposed a method of finding candidate areas for additional conservation spatially. The findings of this study can be a valuable reference for the qualitative improvement of protected areas through the complementarity assessments, including animals and the effectiveness assessment study of protected areas using the National Ecosystem Survey data in the future.

• Journal of Korean Society of Forest Science
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• v.108 no.4
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• pp.618-627
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• 2019

An increase in damage to crops and facilities caused by wildlife such as wild boar causes serious social and economic problems to the rural economy in Korea. This study aims to estimate the economic losses incurred to rural households in mountain villages near protected areas in Korea due to the damage to agricultural and forestry production by wildlife. The case of mountain villages located in Toji-myeon, Gurye-gun near the Jirisan National Park was studied as an example. Data were collected by interviewing 84 households across six mountain villages in April and June 2016. The results indicate that the economic losses due to wildlife damage in 2015 were estimated to be 1.65 million KRW per household, which is a total of 138.63 million KRW for the 84 households. Among local products, the most damaged products were chestnut, fern-brake, wild pear, peach, and potato, whereas the most damaged products per production area were sweet potato, peach, corn, and potato. The potential economic losses caused by wildlife damage to agricultural and forestry production in whole Gurye-gun area was estimated to be around 4.0 billion KRW in 2015. However, the municipal government budget for the compensation of wildlife damage was only 0.9 percent of the potential rural economic losses caused by wildlife damage in 2015. The results of our study suggest that the compensation scheme for wildlife damage is inadequate in the respect of social justice. Considering the low financial capacity of the municipalities in mountain areas, the central government should take a progressive responsibility by allocating more financial resources for compensation of economic losses of rural households due to wildlife damage. The equitable and effective governance of wildlife conservation is required for sustainable development of rural communities near protected areas.

• Journal of agriculture & life science
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• v.50 no.3
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• pp.129-139
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• 2016

Curcumin plays a protective role in brain injury through its anti-oxidant and anti-inflammatory activities. Moreover, peroxiredoxin-5 exerts a protective effect against oxidative stress. The aim of this study was to investigate whether curcumin modulated the peroxiredoxin-5 expression in focal cerebral ischemic animal model. Middle cerebral artery occlusion(MCAO) was performed to induce cerebral ischemic injury in rats. Adult male rats were injected intraperitoneally with vehicle or curcumin(50mg/kg B.W.) 1 h after MCAO and cerebral cortex tissues were collected 24 h after MCAO. Photographs of hematoxylin and eosin staining showed that MCAO induced necrotic changes with scalloped shrunken form and apoptotic changes with nuclear chromatin condensations. However, curcumin treatment attenuated MCAO-induced histopathological changes. Moreover, this study clearly showed that peroxiredoxin-5 expression was decreased in MCAO operated animal with vehicle using a proteomics approach. However, this decrease in peroxiredoxin-5 expression was attenuated by curcumin treatment. Reverse-transcription PCR and Western blot analyses confirmed that curcumin treatment alleviated the MCAO injury-induced decrease in peroxiredoxin-5 expression(p<0.05). These results demonstrated that curcumin regulates peroxiredoxin-5 expression in MCAO animal model. In conclusion, our findings suggest that curcumin exerts a neuroprotective effect in cerebral ischemia by attenuating the MCAO-induced decrease in peroxiredoxin-5 expression.

• Proceedings of the KSAR Conference
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• 2004.06a
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• pp.280-280
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• 2004

본 연구는 개 동결정액 희석액에 pentoxyfilline을 첨가하여 융해 후 정액성상에 미치는 영향을 조사하였다. 공시견은 2∼3세의 Beagle, English cockers spania, Collie, Grey hound, Shihtzu 5두를 공시하였다. 정액은 수압법 또는 인공질법으로 채취하였고 채취된 정액은 Rota 등(EYT : Egg-yolk Tris extender, 1995)의 방법에 따라 동결정액을 제조하였다. Pentoxyfilline은 동결 보호제가 들어 있는 2차 희석액에 0 mM, 1 mM, 3 mM 농도로 첨가하였으며, 5℃에서 2시간 glycerol 평형을 한 후에 액체질소에 10분간 예비 동결하여 동결정 액을 제조하였다. (중략)

• Proceedings of the KSAR Conference
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• 2002.06a
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• pp.3-3
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• 2002

본 연구는 개의 동결정액제조 시 동결보호 희석액 내에 첨가되는 당의 종류와 조합이 동결융해 후 정자의 생존율 및 운동성에 미치는 영향과 정자동결 시 straw size, 예비동결 시 액체질소표면으로부터 straw까지의 거리 및 융해온도가 생존율에 미치는 영향에 대하여 조사하고자 실시하였다. 포유류의 경우 부생식선의 정낭선에서 분비되는 정장내에는 정자의 체외사출 후 정자의 영양물질인 fructose 가 분비되는데, 동결정액제조 시 회석액내에 첨가되는 당 성분은 fructose 및 glucose를 일반적으로 사용하고 있다. (중략)

• Proceedings of the Korea Contents Association Conference
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• 2017.05a
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• pp.187-188
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• 2017

아동에 대한 관심이 높아지면서 미아방지를 위한 다양한 제도와 제품들이 개발되고 있다. 하지만 여전히 미아 발생률은 줄어들지 않고 있다. 선행 연구의 리서치 결과 국내 유원지에서 미아 발생 시 미아를 찾는데 너무 많은 시간이 소요 되고, 마땅한 미아 방지 프로그램이 없어 방송이나 미아 보호센터에만 의존하는 등 비효율적인 시스템의 정황을 파악할 수 있었다. 본 연구에서는 이러한 현 실태를 개선하고 미아 사건의 빠른 해결을 위해 대전 동물원을 대상으로 미아방지에 효과적인 방재디자인 기반의 웹서비스를 연구하고자 한다.