• Title/Summary/Keyword: 독신

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독신여성을 위한 상품기획 연구

  • 이연희;김영인
    • Proceedings of the Costume Culture Conference
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    • 2003.04a
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    • pp.86-87
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    • 2003
  • 통계청이 5년마다 조사하는 전국 인구 센서스에 따르면 2000년 전국 총 1천4백39만 가구 가운데 독신 가구는 2백 22만 가구로 전체 가구 중 15.4%를 차지하며 여성의 가구주는 2천6백 53명으로 48.25%가 혼자 살고 있으며 독신의 비율이 지난 10년 사이에 비해 많은 증가율을 보이고 있다고 보고되었다. 본 연구에서는 새로운 소비자로 등장한 독신, 그 중 여성 독신자를 대상으로 그녀들이 열어 가는 1인 가족시대의 라이프 스타일을 위 한 상품기 획을 제안하고자 하였다. (중략)

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Effects of Mutation at Two Conserved Aspartate Residues and a Serine Residue on Functions of Yeast TSA 1 (Saccharomyces cerevisiae TSA1의 보존된 아스파트산 잔기 및 세린 잔기의 변이가 과산화효소 활성 및 샤페론 활성에 미치는 영향)

  • Lee, Songmi;Cho, Eun Yi;Kim, Kanghwa
    • Microbiology and Biotechnology Letters
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    • v.45 no.1
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    • pp.81-86
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    • 2017
  • Alignment of 967 reference sequences of the typical 2-Cys peroxiredoxin family of proteins revealed that 10 amino acids were conserved, with over 99% identity. To investigate whether the conserved aspartic acid residues and serine residue affect the peroxidase and chaperone activity of the protein, we prepared yeast TSA1 mutant proteins in which aspartic acids at positions 75 and 103 were replaced by valine or asparagine, and serine at position 73 was replaced by alanine. By non-reducing SDS-PAGE, TSA1 and the S73A, D75V and D75N mutants were detected in dimeric form, whereas the D103V and D103N mutants were detected in various forms, ranging from high molecular-weight to monomeric. Compared with wild type TSA1, the D75N mutant exhibited 50% thioredoxin peroxidase activity, and the S73A and D75V mutants showed 25% activity. However, the D103V and D103N mutants showed no peroxidase activity. All proteins, except for the D103V and D103N mutants, exhibited chaperone activity at $43^{\circ}C$. Our results suggest that the two conserved aspartic acid residues and serine residue of TSA1 play important roles in its thioredoxin peroxidase activity, and D103 plays a critical role in its chaperone activity.

An Analysis and Comparison of the Single and the Married: Their form of Using the Washing Machine (독신 미혼와 기혼의 세탁기 사용 형태 비교 분석)

  • Yoon, Hyung-Kun
    • Archives of design research
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    • v.19 no.1 s.63
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    • pp.263-272
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    • 2006
  • People's values as well as their behavioral form of using a product are changing due to the rapid introduction of diverse cultures and the Five-day workweek system. The single and the married are the subjects of this study: it is aimed to evaluate and analyze, thus grasping their difference in behavioral form of using a washing machine, the main factors they consider to be important when purchasing one, and the level of satisfaction they get from their own washers. A questionnaire was used as a tool for a better Factor Analysis and Frequency Analysis research. There were 6 essential factors which unmarried people take into consideration when purchasing a washer: economical efficiency, measure of capacity, design, type, brand and advertising, function. On the other hand, economical efficiency, convenience, measure of capacity, function, and type was what the married thought would be the 6 central factors.

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Leisure Experiences of Unmarried Women (독신여성들의 여가체험 이해)

  • Kim, Yee-Jeung
    • The Journal of the Korea Contents Association
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    • v.9 no.1
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    • pp.359-365
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    • 2009
  • The purpose of this study was to investigate the meaning of leisure experiences in the lives of unmarried women. To accomplish this purpose, this study focused on the cultural context of leisure activity of unmarried women. A total of 4 participants were selected through the process of purposive sampling. An ethnographic data was collected by the method of 'experiential description' phenomenologically based data on the leisure experience, participant observation, and in-depth interview. All verbal data from experiential description, participation observation, and in-depth interview were tape recorded and later transcribed, and analyzed through constant comparative method based on the phenomenological analysis. The following findings were the result of this study. three definitional components of leisure experience of unmarried women were identified as follows: 1) enjoyment and interest, 2) escape, and 3) freedom.

Characterization of the Interaction of Sulfiredoxin (Srx1) with a Vacoular Protein $\alpha$-Mannosidase (Ams1) in Saccharomyces cerevisiae (설피리독신과 알파-만노시다제 간의 단백질 결합 특성에 관한 고찰)

  • Barando, Karen P.;Kim, Il-Han
    • The Journal of Natural Sciences
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    • v.17 no.1
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    • pp.13-29
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    • 2006
  • Most redox-active proteins have thiol-bearing cysteine residues that are sensitive to oxidation. Cysteine thiols oxidized to sulfenic acid are generally unstable, either forming a disulfide with a nearby thiol or being further oxidized to a stable sulfinic acid, which have been viewed as an irreversible protein modification. However, recent studies showed that cysteine residues of certain thiol peroxidases (Prxs) undergo reversible oxidation to sulfinic acid and the reduction reaction is catalyzed by sulfiredoxin (Srx1). Specific Cys residues of various other proteins are also oxidized to sulfinic acid ($Cys-So_2H$). Srxl is considered one of the oxidant proteins with a role in signaling through catalytic reduction of oxidative modification like in the reduction of glutathionylation, a post-translational, oxidative modification that occurs on numerous proteins. In this study, the role of sulfiredoxin in cellular processes, was investigated by studying its interaction with other proteins. Through the yeast two-hybrid system (Y2HS) technique, we have found that Ams1 is a potential and novel interacting protein partner of Srxl. $\alpha$-mannosidase (Ams1) is a resident vacuolar hydrolase which aids in recycling macromolecular components of the cell through hydrolysis of terminal, non-reducing $\alpha$-D-mannose residues. It forms an oligomer in the cytoplasm and under nutrient rich condition and is delivered to the vacuole by the Cytoplasm to Vacuole (Cvt) pathway. Aside from the role of Srxl as a catalyst in the reduction of cysteine sulfenic acid groups, it may play a completely new function in the cellular process as indicated by its interaction with Ams1 of the yeast Saccharomyces cerevisiae.

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An Exploratory Study on the Attitudes and the Perceptions Toward a Single Life (독신에 대한 인식과 만족도에 관한 탐색적 연구)

  • 박충선
    • Journal of Families and Better Life
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    • v.20 no.6
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    • pp.163-171
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    • 2002
  • The purpose of this study is to examine the attitudes and the perceptions toward a single life and life satisfaction among male and female singles. In this study, a total of 160 singles aged 30 to 39 were interviewed using a questionnaire on socio-economic status, the attitudes and perceptions toward a single life, and life satisfaction. The data were analyzed using frequencies, percentages and chi-square. The major findings of this study are as follows; 1) the socio-economic status of singles are higher than that of the married ones, showing a high level of income, education, and jobs. The 54.4% of the respondents showed that they remained as singles, because they have not met desirable partners yet, and the 20.9% answer that the main reasons for being a single are working and studying. The attitudes and the perceptions toward a single life were measured in six areas; freedom, self-achievement, self-development, institutional pressure. anxiety of support systems, and sex discrimination toward singles. The freedom of a single life was shown to have significant relationships with education and income. The self-achievement, self-development, and the level of anxiety about support systems were shown to be related to the sex of singles, indicating that single women got more benefits than single men. Single men experienced more institutional pressures such as marriage pressures and filial piety than single women. There was no difference in the level of acceptance to live as a single in terms of sex. Finally, life satisfaction with a single life turned to have significant relationships with sex, age, education, and income.

An Exploratory Study on the Economic Life of Single Households (독신가구의 경제생활에 대한 탐색적 연구)

  • Shim, Young
    • Journal of Families and Better Life
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    • v.20 no.6
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    • pp.197-208
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    • 2002
  • The purpose of this study was to investigate the economic lives of single households with the age of 30 through less than 50. The economic life was examined in three aspects; income, assets, and consumption expenditures and patterns. One hundred sixty single households were surveyed, with questionnaires for 15days from April 1 to April 15 of 2002, and analyzed with descriptive statistics. The results were as follows: about 30.8% of total single households in the sample were in the income group of 1,500,000 thought less than 2,000,000 won. The size of income for the 30's was less than that for the 40's. Compared with male single households, female single households were more in both low and high levels of income. The saving rate tended more or less to be low. The more the age, the higher the saving rate. As for debt, the 40's single households, male single households, high school single households (compared to the 30's single households, female single households, university and graduate single households, respectively) were relatively higher. In general, the single households tended to have debts due to preparation for housing, credit over use. The assets tended to be managed by themselves. The economic preparation for the old life was done by banking system rather than insurance. The average monthly living costs was higher in the age of the 40's single than the age of the 30's single. The living costs of the female single households was higher or lower than those of the male single households. As for consumption patterns, there was the most in the expenditure allocation for food away from home, then for culture entertainmentㆍsocial life, and for clothing and shoes. As for the convenience of the consumption life in overall, there were more responses in moderation and inconvenience than in convenience.

A study on the types of unmarried single women based on their subjective perceptions on single life (독신 여성의 싱글생활에 대한 주관적 인식의 유형화 연구)

  • Wang, Seok Soon;Ryu, Kyung Hee
    • Korean Journal of Human Ecology
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    • v.22 no.6
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    • pp.615-631
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    • 2013
  • In this study, we attempt to explore various possible types of unmarried single women. Main purpose of the work is to find out possible types of unmarried single women in terms of their subjective views on single life based on Q methodology. Q methodology is a research method to study and understand people's "subjectivity" or their points of view. Here, we applied Q methodology to unmarried single women's subjective evaluation of their own lives. Thirty-one Q-questions were prepared through literature review, and were offered to eighteen unmarried single women. As a result, the subjects could be classified into the following five different types: (Type 1) 'I love my work but I hate pressure (for marriage) from others', (Type 2) 'I feel anxiety and stress (on marriage) but I am still waiting for a true love of mine', (Type 3) 'My life is most important and I'd rather enjoy my single life without restraint from others', (Type 4) 'I am weary of continuing my single life, and now I really want to get married and form a family', (Type 5) 'I don't feel comfortable with dating, marriage life etc, I'd rather continue my single life with less stress'.