• Title/Summary/Keyword: 대한생명

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Agrobacterium-mediated genetic transformation of Trichoderma sp. KACC 40541 (Agrobacterium을 이용한 Trichoderma sp. KACC 40541의 형질전환)

  • Choi, Jang-Won;Park, Hee-Sung
    • Journal of agriculture & life science
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    • v.45 no.1
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    • pp.119-124
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    • 2011
  • Trichoderma spp. are very important as being a major source for the industrial production of various secreting enzymes in the field of white biotechnology. In this work, Agrobacterium-mediated transformation (AMT) was studied using Trichoderma sp. KACC 40541 which has high activities of amylase, pectinase, cellobiohydrolase and xylanase. In principal, optimized NaOH treatment, prior to Agrobacterium infection, to the mycelium was determined to be very effective for AMT.

A Transcendental Pragmatic Interpretation on the Notion of 'Injon' in Daesoon Thought (대순사상의 인존(人尊)에 대한 화용론적(話用論的) 해석)

  • Baek, Choon-hyoun
    • Journal of the Daesoon Academy of Sciences
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    • v.39
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    • pp.33-67
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    • 2021
  • This paper aims at revealing the core concept of Injon (Human Nobility). The concept of Injon is one of the salient fundamental ideas which makes Daesoon Jinrihoe recognizable as Daesoon Jinrihoe. The concept of Injon has the basic meaning of 'human nobility,' but within the context wherein the nobility of humankind is considered to be greater than the nobility of Heaven and Earth. Although the religious and ideological interpretations of Injon (human nobility) that have developed over time have been quite diverse and abundant, these interpretations are all limited in that they generally assume the relationship between 'Heaven and Earth' and 'Humanity' to be antagonistic. However, if human nobility is relativized in that manner, it can reduce the potential broader meanings of mutual beneficence and the earthly paradise of the later world. These interpretations are grounded in the view of semiotic interpretation. Such interpretations have composed their view point via the semiotic meaning of the words. The semiotic point of view suggests that meanings of words consist in the relation of the word and the object to which it denotes. We will introduce a new view point which can be termed the transcendental view point. This view focuses on how the exact interpretation of words and sentences depends on the comprehension of the triad of systematic relations among the word, object, and speaker. In the Daesoon Thought, the Former World is considered to be the world wherein all creations unfolded according to the principle of mutual contention. This led to the accumulation of grievances and grudges which condensed and filled the Three Realms of Heaven, Earth, and Humanity. The Former World was dominated by Western material civilization, selfishness, and exclusivism. It was also a world where humans suffered from various natural disasters such as floods, droughts, plagues, and wildfires. The Former World lost the constant Dao and was overwhelmed with all kinds of disasters and calamities. That world fell into various kinds of wretchedness. The causes which made the Former World so cruel came from humans misunderstanding their relation to nature and life in general; including human life. The anthropocentric modern cosmology insisted that the human race was the only one to have the powers and rights to exercise dominion over nature. On the other hand, there is the Later World, which means the ideal and perfect, immanent eternal world for all humankind in Daesoon Thought. This world consists of life, peace, and equality and is also characterized by three typical attributes: goodness, peace, and all kinds of life. All living beings previously struggled for survival, but in the Later World, those lifeforms will embrace each other; even across different realms. In Daesoon Thought, the world and cosmos contain diverse forms of life, and human have both an earthly life and life in the after world should they die before the Later World. There are also the lives of divine beings and animals, and other such living entities. Daesoon Thought subsumes pan-vitalism, which allows they acknowledgement of myriad possible lifeforms. The concept of the Later World in Daesoon Thought, which mainly revealed in The Canonical Scripture and the words of Sangje (Kang Jeungsan), suggests that all kinds of life, including humans, animals, and even spirits in the afterworld, can live together in a perfect coming earthly paradise which is immanent. The concept of Injon can be interpreted though the view of transcendental pragmatics as an alternative to the typical views discussed in Daesoon Thought. Thinkers should attempt to improve current discourse on Injon in Daesoon Thought by focusing on the point that all kinds the original teachings demonstrate a value of all lifeforms. Therein, Injon would indicate not only the human nobility and dignity but also the nobility and dignity of divine beings, divine humans, and all other forms of life that have existed across time. The dimension of time allows for recognition of lifeforms from the Former World, the afterworld, and the Later World. This revised appraisal of Injon could further accommodate denizens of the afterworld, animals, ghosts and spirits, the earth and cloud souls of humans, and other lifeforms held to exist in the cosmology of Daesoon Thought.

Generation of Bacterial Blight Resistance Rice with Transcription Factor OsNAC69-overexpressing (전사인자 OsNAC69-과발현을 통한 흰잎마름병 저항성 벼 제작)

  • Park, Sang Ryeol;Cha, Eun-Mi;Moon, Seok Jun;Shin, Dongjin;Hwang, Duk-Ju;Ahn, Il-Pyung;Bae, Shin-Chul
    • Korean Journal of Breeding Science
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    • v.43 no.5
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    • pp.457-463
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    • 2011
  • Plant specific gene family, NAC (NAM, ATAF, and CUC) transcription factors have been characterized for their roles in plant growth, development, and stress tolerance. In this study, we isolated OsNAC69 gene and analyzed expression level by inoculation of bacterial leaf blight pathogen, Xanthomonas oryzae pv. oryzae (Xoo). NAC transcription factor family can be divided into five groups (I-V). On the basis of phylogenetic analysis, OsNAC69 was fall into group II. OsNAC69 was strongly induced 1 hr after infected with Xoo. To investigate its biological function in the rice, we constructed vector for overexpression in rice, and then generated transgenic rice lines. Gene expression of OsNAC69-overexpressed transgenic rice lines were analyzed by northern blot. Analysis of disease resistance to pathogen Xoo, nine OsNAC69-overexpressed transgenic rice lines showing high expression level of OsNAC69 were shown more resistant than wild type. These results suggest that OsNAC69 gene may play regulatory role during pathogen infection.

A Comparative Study on Morphologies and Characteristics of Silica Nanoparticles Recycled from Silicon Sludge Waste of Semiconductor Process Based on Synthesis Methods (반도체 공정에서 발생하는 폐실리콘 슬러지의 재활용을 통한 실리카 나노입자의 제조 및 합성법에 따른 형상 및 특성 비교 연구)

  • Jiwon Kim;Minki Sa;Yeon-Ryong Chu;Suk Jekal;Ha-Yeong Kim;Chan-Gyo Kim;Hyung Sub Sim;Chang-Min Yoon
    • Journal of the Korea Organic Resources Recycling Association
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    • v.31 no.3
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    • pp.5-13
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    • 2023
  • In this study, a comparative study is conducted on the synthesis methods for silica nanoparticle employing the silicon sludge waste generated from the semiconductor manufacturing processes. Specifically, acid-washed silicon sludge wastes with no impurities are employed as the precursors of sol-gel and hydrothermal methods for silica nanoparticles preparation. The morphologies and properties of silica nanoparticles synthesized via two synthetic methods are examined by various analysis methods. As a result, silica nanoparticles from the sol-gel method are fabricated with high purity and uniform shape, while the hydrothermal method exhibits advantages in yield and ease of synthetic process. This comparative study offers detailed experimental results on the two synthetic methods for silica nanoparticle synthesis, which may contribute to the establishment of manufacturing high-value materials using the by-products generated in the semiconductor process.

A Study on Anti-inflammatory and Skin Soothing Cosmetics Material of COMPAREX (COMPAREX 의 항염 및 진정 화장품 소재로서의 연구)

  • Ha Yeon Kim;Jae Seok Lee;So Hun Lee;Ye Hyang Kim;Eun Young Jeong;Kang Hyuk Lee;Song Seok Shin
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.49 no.3
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    • pp.269-276
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    • 2023
  • Recently, there has been a growing interest in the development of safer and more effective soothing materials to calm skin that has become sensitive to various external factors. The aim of this study was to investigate the skin soothing and anti-inflammatory effects of COMPAREX compared to Portulaca Oleracea extract. The results showed that COMPAREX significantly inhibited LPS-induced NO production and the expression of inflammatory factors iNOS, COX-2, TNF-α, and IL-6 more than P. oleracea extract. In addition, COMPAREX has been confirmed to have a more effective sedative effect by further inhibiting the gene and protein expression of IL-1α against SDS stimulation than the Portulaca Oleracea extract. Furthermore, COMPAREX inhibited the expression of inflammatory factors COX-2 and IL-8 increased by PM2.5 and suppressed H2O2-mediated carbonylated protein in hair cell. These results suggest that COMPAREX has shown the potential to be used as an improved natural soothing material over P. oleracea extract, and it is expected to be used as a derma cosmetic material in the future.

The Regulation of Stress Responses by Non-tandem CCCH Zinc Finger Genes in Plants (식물에서 non-tandem CCCH zinc finger 그룹 유전자에 의한 스트레스 반응 조절)

  • Hye-Yeon Seok;Md Bayzid;Swarnali Sarker;Sun-Young Lee;Yong-Hwan Moon
    • Journal of Life Science
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    • v.33 no.11
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    • pp.956-965
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    • 2023
  • In plants, there are many CCCH zinc finger proteins consisting of three cysteine residues and one histidine residue, which bind to zinc ions with finger configuration. CCCH-type zinc finger proteins are divided into tandem CCCH-type zinc finger (TZF) and non-TZF proteins: TZF proteins contain exactly two tandem CCCH-type zinc finger motifs whereas non-TZF proteins have fewer or greater than two CCCH-type zinc finger motifs. The functions of TZF genes, especially plant-specific RR-TZF genes, have been well studied in several plants, whereas the functional roles of non-TZF genes have not been adequately researched compared to TZF genes. Many non-TZF genes have been identified as being involved in the responses to biotic and abiotic stresses, such as pathogen, high salt, drought, cold, heat, and oxidative stresses. Some non-TZF proteins bind to RNA and are involved in the post-transcriptional regulation of stress-responsive genes in the cytoplasm. In addition, other non-TZF proteins act as transcriptional activators or repressors that regulate the expression of stress-responsive genes in the nucleus. Despite these studies, stress signal transduction and upstream and downstream genes of non-TZF genes have not been sufficiently researched, suggesting that additional studies of the functions of non-TZF genes' functions in plants' stress responses are needed. In this review, we describe non-TZF genes involved in biotic abiotic stress responses in plants and their molecular functions.

Thermal Comfort Condition of Temperature and Humidity in Loess Interior Space

  • Kong, Sung-Hoon
    • Biomedical Science Letters
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    • v.10 no.2
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    • pp.129-135
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    • 2004
  • The study was carried out through measuring the temperature and humidity of the indoor/outdoor space and the distribution of interior thermal condition, and investigating the effect of loess materials on human body. The purpose of this study is to analyze the change of dry bulb temperature and relative humidity and correlation of thermal reaction of human body with ASHRAE (American Society of Heating, Refrigerating and Air-conditioning) comfort chart in the loess interior space. In the view point of biomedical sciences, loess interior space provides optimum thermal conditions for human thermal sensation.

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독사독의 Inhibitor에 관한 연구

  • 이동의;서정훈;방병호
    • Proceedings of the Korean Society for Applied Microbiology Conference
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    • 1976.10a
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    • pp.189.1-189
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    • 1976
  • 본인등이 Agirtrodon halys blomohoffi 및 Trime-resurus flavoviridis 독에 대한 inhibitor를 미생물계에서 분리코져 시도한 것은 1966년경 부터였으며 그 후 1971년에 inhibitor 2종을 분리하게 되었으며 이 2종의 inhibitor는 그 작용상이 대단히 유사하나 금속에 대한 성질, 그 정제성등에 다소 차이를 나타내었다. 금반에는 이 2종 inhibitor의 작용조건 및 그 작용상에 대해서 발표코저 한다.

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Screening Test of Wild Mushroom Extracts for Fibrinolytic Activity

  • Kim, Jun-Ho
    • Biomedical Science Letters
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    • v.8 no.3
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    • pp.173-177
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    • 2002
  • To investigate the fibrinolytic activities of the Korean basidiomycetes, the Extracts of 50 wild mushrooms were tested for their fibrinolytic activites. Extract from Tricholoma sejunctum showed 175% increased activity to that of plasmin 1.0 U/ml. Marasmius siccus showed 54% of activity, and Laetiporus sulphureus var. miniatus and Macrolepiota procera, 43% and 26% activities, respectively to that of plasmin. But, Cystoderma amianthinum, Lepiota sp., Coprinus sp., Lycoperdon sp. were less than 10% of plasmin activity.

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수소생성 광합성 세균 Rhodopseudomonas strain K-7의 색소 생성능에 대한 연구

  • Na, Young-Mi;Bae, Moo
    • Proceedings of the Korean Society for Applied Microbiology Conference
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    • 1986.12a
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    • pp.518.1-518
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    • 1986
  • 광합성 세균의 수소생성 기작에 대한 연구의 일환으로 광합성 세균에서 생성되는 색소의 성분을 조사하였다. 분리한 광합성 세균 K-7은 수소생성능이 뛰어난 균주로 조사된 홍색 비유황세균으로서 생리, 형태 및 배양학적 조사에 의하여 Rhodopseudomonas spheroides로 분류하였다. Type culture인 Rhodopseudomonas spheroides NCIB 8253과 비교 연구하였으며, SEM(Scanning Electron Microscope)하에서 기존균주와 분리균주의 형태학적인 특징을 확인하였다. 군 동정의 주요열쇠가 되는 색소성분을 조사한 결과, 균주 K-7에서 추출된 carotenoids로는 spheroidene의 산화형태인 OH-spheroidenone이 주성분이었고, Neurosporene, Lycopene, Anhydro- rhodovibrin, Rhodovibrin등이 동정되었다.

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