• Journal of the Korea Academia-Industrial cooperation Society
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• v.11 no.3
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• pp.971-977
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• 2010

A flood of biological data has caused many challenges in computing. Bioinformatics, the application of computational techniques to analyze the information associated with biomolecules on a large-scale, has now firmly established itself as an interdisciplinary subject in molecular biology, and encompasses a wide range of subject areas from structural biology, genomics, proteomics, systems biology, biostatistics to computer science. In this review, I provide an introduction and overview of the current state of bioinformatics. Looking at the types of biological information and databases that are commonly used, I also deals with some of bioinformatics application domains which are closely related to areas of computer science.

• Journal of agriculture & life science
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• v.44 no.5
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• pp.75-79
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• 2010

Apple, one of the most important fruit crops world-widely, is required for rapid, cost-effective and sensitive virus detection for its better productivity. RT-PCR is extensively employed for apple virus diagnosis, but the technique requires complete tissue homogenization which is time consuming and laborious. In this study, heating-based RNA extraction method was developed and proven to effectively work for stem tissues slightly better than for leaf tissues. In RT-PCR, almost identical results were generated from the use of RNA extracts from both tissues.

• Journal of agriculture & life science
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• v.44 no.6
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• pp.141-146
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• 2010

In this study, Agrobacterium-mediated transformation was tested to the mycelium culture of Flamulina velutipes which is very popular as an edible mushroom in Korea. Particularly, aluminum oxide particles were used to generate wounds in F. velutipes mycelia via vigorous shaking prior to agro-infiltration. The result showed that transformants resistant to hygromycin could be obtained only from the mycelia with physical wounds. Gene transfer was verified by genomic DNA PCR. This study suggested a convenient tool to improve Agrobacterium-mediated transformation of F. velutipes.

• Journal of fish pathology
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• v.31 no.2
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• pp.123-129
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• 2018

Parvicapsula anisocaudata, a myxosporean parasite, is presumably one of causative agents of emaciation in olive flounder Paralichthys olivaceus in Korea. In this study, we report a case of unusual abdominal distension due to exceptionally enlarged liver in farmed olive flounder. For the identification of the causative agent, bacteria and nucleic acids of virus that are possibly present were attempted to isolate from internal organs of five fish sampled from a fish farm in Jeju. Although a few bacterial colonies were isolated from some samples, there was no evidence that fish were primarily affected by virus and/or bacteria. From histopathological analysis, myxosporean were found in almost all internal organs, particularly in the stomach. The causative agent was identified as P. anisocaudata by sequencing a part of small subunit rRNA. This study contains a very unusual case of olive flounder heavily and systemically infected with P. anisocaudata, showing excessively enlarged liver with a small amount of ascitic fluid.

• Proceedings of the Korea Water Resources Association Conference
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• 2019.05a
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• pp.358-358
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• 2019

도시화 및 산업화로 인해 불투수면이 지속적으로 증가하고 있으며, 불투수면의 증가로 인해 물순환 구조 왜곡이 발생하고 있으며, 이로 인해 집중 호우시 첨두유량 증가로 인한 도시 침수, 자연적 침투량 감소로 인한 지하수 고갈 및 하천 건천화, 도시 열섬 현상 등 다양한 물 문제가 발생하고 있다. 건강한 물순환 체계 회복을 위해, 환경부에서는 소권역별 불투수면적률에 대한 중장기 물순환 목표를 포함하여 물환경보전법을 개정하였으며, 2020년부터 시행예정이다. 본 연구에서는 안성천 유역을 대상으로 소권역별 불투수면적률을 산정하고 불투수면적률 저감에 따른 유출량 등의 유역 물순환 구조 변화를 분석하고자 한다. 불투수면적률은 연속수치지도, 연속지적도, 용도지역 지구도 3개의 지리정보자료를 중첩하여 최신 불투수면적 정보가 반영되도록 계산하였으며, 물순환 구조는 HSPF 모형을 이용하여 분석하였다. 본 연구의 결과는 향후 불투수면적과 물순환 요소간의 상관관계 분석을 위한 기초자료로 활용될 수 있을 것으로 사료된다.

• Journal of fish pathology
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• v.34 no.1
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• pp.111-115
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• 2021

Immunoglobulin M (IgM) of sevenband grouper (Epinephelus septemfasciatus) was purified by mannan-binding protein (MBP) affinity column. The purified IgM had an apparent molecular weights of 76 (heavy chain) and 28 (light chain) kDa in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Eight hybridoma clones secreting monoclonal antibodies (mAbs) against sevenband grouper IgM were established. Antibody detection enzyme-linked immunosorbent assay (ELISA) with bovine serum albumin (BSA, antigen) and the 8 mAbs revealed that optical density (OD) values were clearly different between sera from BSA-immunization and non-immunization of sevenband grouper. These results suggest that the produced mAbs in this study are specifically reacted with IgM of sevenband grouper.

• Korean journal of applied entomology
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• v.62 no.1
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• pp.49-56
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• 2023

We first report 4 unrecorded species belonging to genus Aleiodes Wesmael discovered in South Korea: A. lipwigi, A. guidaae, A. subemarginatus, A. caprinus. In this study, diagnosis, distribution, and illustration, DNA barcode sequence are provided for identification of these species.

• Journal of the Korean Data and Information Science Society
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• v.25 no.6
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• pp.1207-1219
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• 2014

In this study, 1) we compare face channel and non-face channel of life insurance company and non-life insurance company with insurance employs' suitability opinion about channel type, channel property, channel evaluation items requiring when selling insurance products, 2) we construct two social networks for life insurance companies and non-life insurance companies and find/compare two networks' properties, and then want to suggest any direction about sale channel strategy. As the result of comparing social networks of life insurance company and non-life insurance company created by insurance selling channel fit evaluation, employs of life insurance companies have more common opinion than those of non-life insurance companies and so can have more same directional channel strategy. However, property insurance companies need to manage their own channel strategy based on their own circumstance.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.6
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• pp.4048-4055
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• 2015

The purpose of this study is to examine the awareness of biomedical ethics of long-term care hospital nurses. A survey research was conducted in this study with 183 nurses that worked for over five months at long-term care hospitals located in Gwangju and Jeonnam region. Using SPSS 21.0 program, collected data were analyzed through descriptive statistics, independent t-test and one-way ANOVA. $Scheff{\acute{e}}$ test and Pearson's correlation analysis were also used for post hoc test. In terms of the awareness of biomedical ethics of the research participants, it was at an above average level. Among the sub-categories of biomedical ethics, awareness of right of life of fetus and awareness of artificial abortion showed most meaningful correlation. It would be necessary to provide bioethics education to new nurses or university students majoring in Nursing at related organizations or college of nursing to help them establish ethical values to seek patients' well-being.

• Bulletin of the Korean Chemical Society
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• v.20 no.9
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• pp.1078-1084
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• 1999

A synthetic cecropin A(1-13)-melittin(1-13) [CA-ME] hybrid peptide was known to be an antimicrobial peptide having strong antibacterial, antifungal and antitumor activity with minimal cytotoxic effect against human erythrocyte. Analogues were synthesized to investigate the influences of the flexible hinge region of CA-ME on the antibiotic activity. Antibiotic activity of the peptides was measured by the growth inhibition against bac-terial, fungal and tumor cells and vesicle-aggregating or disrupting activity. The deletion of Gln-Gly-Ile (P1) or Gly-Gln-Gly-Ile-Gly (P3) from CA-ME brought about a significant decrease on the antibiotic activities. In contrast, Gly-Ile-Gly deletion (P2) from CA-ME or Pro insertion (P5) instead of Gly-Gln-Gly-Ile-Gly of CA-ME retained antibiotic activity. This result indicated that the flexible hinge or β-bend structure provided by Gly-Gln-Gly-Ile-Gly, Gln-Gly, or Pro in the central region of the peptides is requisite for its effective antibiotic activity and may facilitate easily the hydrophobic C-terminal region of the peptide to penetrate the lipid bilayers of the target cell membrane. In contrast, P4 and P6 with Gly-Gln-Gly-Pro-Gly or Gly-Gln-Pro in the central region of the peptide caused a drastic reduction on the antibiotic activities. This result suggested that the con-secutive β-bend structure provided by Gly-Gln-Gly-Pro-Gly or Gly-Gln-Pro in the central hinge region of the peptide seems to interrupt the ion channel/pore formation on the target cell membranes.