Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Production of monoclonal antibodies to immunoglobulin M of sevenband grouper (Epinephelus septemfasciatus)

능성어(Epinephelus septemfasciatus) immunoglobulin M에 대한 단클론 항체 생산

  • Kim, Si-Woo (Department of Aqualife Medicine, Chonnam National University) ;
  • Kim, Jong-Oh (Institute of Marine Life Science, Pukyong National University) ;
  • Kong, Kyoung-Hui (Department of Aqualife Medicine, Chonnam National University) ;
  • Oh, Myung-Joo (Department of Aqualife Medicine, Chonnam National University) ;
  • Kim, Wi-Sik (Department of Aqualife Medicine, Chonnam National University)
  • 김시우 (전남대학교 수산생명의학과) ;
  • 김종오 (부경대학교 해양생명과학연구소) ;
  • 공경희 (전남대학교 수산생명의학과) ;
  • 오명주 (전남대학교 수산생명의학과) ;
  • 김위식 (전남대학교 수산생명의학과)
  • Received : 2021.05.31
  • Accepted : 2021.06.08
  • Published : 2021.06.30
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Abstract

Immunoglobulin M (IgM) of sevenband grouper (Epinephelus septemfasciatus) was purified by mannan-binding protein (MBP) affinity column. The purified IgM had an apparent molecular weights of 76 (heavy chain) and 28 (light chain) kDa in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Eight hybridoma clones secreting monoclonal antibodies (mAbs) against sevenband grouper IgM were established. Antibody detection enzyme-linked immunosorbent assay (ELISA) with bovine serum albumin (BSA, antigen) and the 8 mAbs revealed that optical density (OD) values were clearly different between sera from BSA-immunization and non-immunization of sevenband grouper. These results suggest that the produced mAbs in this study are specifically reacted with IgM of sevenband grouper.

Keywords

Acknowledgement

이 논문은 2015년도 해양수산부 재원으로 해양수산과학기술진흥원의 지원을 받아 수행된 '수산동물 바이러스 전염병 진단용 항체 생산(20150259)' 연구임.

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