• Journal of Life Science
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• v.33 no.7
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• pp.593-604
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• 2023

The ocean accounts for over 70% of the Earth's surface and is a space of largely unexplored unknowns and opportunities. Korea is a peninsula surrounded by the sea on three sides, emphasizing the importance of marine research. The ocean has an extremely complex environment with immense biological diversity. In terms of microbiology, the marine environment has varying factors like extreme temperature, pressure, solar radiation, salt concentration, and pH, providing ecologically unique habitats. Due to this variety, marine organisms have very different phylogenetic classifications compared with terrestrial organisms. Although various microorganisms inhabit the ocean, studies on the diversity, isolation, and cultivation of marine microorganisms and the secondary metabolites they produce are still insufficient. Research on bioactive substances from marine microorganisms, which were rarely studied until the 1990s, has accelerated in terms of natural products from marine Actinomycetes since the 2000s. Since then, industries for bioplastic and biofuel production, carbon dioxide capture, probiotics, and pharmaceutical discovery and development of antibacterial, anticancer, antioxidant, and anti-inflammatory drugs using bacteria, archaea, and algae have significantly grown. In this review, we introduce current research findings and the latest trends in life science and biotechnology using marine microorganisms. Through this article, we hope to create consumer awareness of the importance of basic and applied research in various natural product-related discovery fields other than conventional pharmaceutical drug discovery. The article aims to suggest pathways that may boost research on the optimization and application of future marine-derived materials.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.3
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• pp.429-437
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• 2019

This study was a descriptive study to investigate the factors influencing on attitude toward withdrawal of life-sustaining treatment of nursing students' consciousness of biomedical ethics, good death recognition and meaning of life. The subjects of this study were 293 nursing students attending college at P city. Data were collected for two weeks from May 1, 2018 to May 11, 2018. The statistical method was performed with SPSS WIN 22.0 using a complex sampling analysis, pearson's correlation coefficients, and a multiple regression analysis. The result of this study was significant positive correlation between attitude toward withdrawal of life-sustaining treatment and consciousness of biomedical ethics(r=.266, p<.001), good death recognition(r=.373, p<.001), meaning of life(r=.122, p=.037). The meaning of life was significant positive correlation consciousness of biomedical ethics(r=.294, p<.001), good death recognition(r=.230, p<.001). The good death recognition was significant positive correlation consciousness of biomedical ethics(r=.306, p<.001). Factors Influencing on the attitude toward withdrawal of life-sustaining treatment was good death recognition(${\beta}=.32$, p<.001) and consciousness of biomedical ethics(${\beta}=.16$, p=.004). These factors explained about 16% of the variance. Therefore it is necessary to develop an educational program to positively raise the attitude of consciousness of biomedical ethics and good death recognition.

• Journal of agriculture & life science
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• v.50 no.2
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• pp.125-138
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• 2016

To assess the industrial possibility of mixed-extracts containing Hovenia dulcis Thunberg and 12 different botanical ingredients, a protective effect was confirmed in the chronic ethanol-induced the liver, brain, and blood injury in mouse. Blood glucose levels of the normal control group(NG) and ethanol administration group(EG) were respectively 119.43mg/dL and 305.25mg/dL, and the mixed-extracts administration group(100, 200mg/kg body weight + 25% ethanol 5g/kg body weight respectively; ME100 & ME200) were decreased to 272.76mg/dL and 234.60mg/dL. Blood ethanol contents were decreased in ME100 and ME200(3.85mg/dL, 3.08mg/dL) compared to EG(4.08mg/dL), and blood acetaldehyde contents were also decreased in ME(15.76mg/dL, 15.16mg/dL) compared to EG(18.72mg/dL). The contents of hepatotoxic indicators such as glutamine pyruvic transaminase(GPT) and glutamic oxaloacetic transaminase (GOT), nephrotoxic indicators such as blood urea nitrogen(BUN), and creatine(CRE), and total cholestero(TCHO), and triglyceride(TG) in mouse blood serum were significantly decreased in the ME compared to EG. The acetylcholinesterase(AChE) activity of ME(109.00% and 108.47%, respectively) in mouse brain tissues was decreased in ME compared to EG(116.10%). Finally, ME was remarkable in vivo antioxidant activities in the mouse liver and brain tissues by superoxide dismutase(SOD), oxidized glutathione(GSH)/total GSH ratio and the malondialdehyde (MDA) assay. Therefore, the mixed-extracts was considered to be effective a high value food with protective effect against chronic ethanol traetment-induced cytotoxicity in liver and brain tissues.

• Journal of Digital Convergence
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• v.14 no.11
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• pp.407-416
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• 2016

The purpose of this study was to identify the awareness of biomedical ethics and attitudes toward human tissue donation and transplantation among Nursing students and General college students. The study was conducted on 2016 May 1st to June 10th with 521 students at two universities. Study results showed awareness of biomedical ethics score of nursing students was 2.16 and general college students 2.01, which was statistically significant difference. Attitude scores towards human tissue donation and transplantation were 2.52 and 2.50, not statistically significant (t=4.671, p=.000). Study results indicate the need to establish biomedical ethics preferred curriculum. It also seems necessary to offer students related systematic training program in order to increase the awareness of bio-medical ethics.

• The Korea Journal of Herbology
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• v.29 no.3
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• pp.19-26
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• 2014

Objectives : Aucklandiae Radix (Muxiang) one of important herbal medicines in oriental medicine, is defined as the dried root of Aucklandia lappa (Asteraceae). Owing to the similarities in the morphology and name, Inulae Radix (Tu-Muxiang) and Vladimiriae Radix (Chuan-Muxiang) as well as Aristolochiae Radix (Qing-Muxiang) originated from other medicinal plants are often used as substitutes and/or adulterants of Aucklandiae Radix. Therefore, a reliable authentication of these herbal medicines is necessarily for the public health and prevention of misuse. Methods : 32 samples of medicinal plants supplying Aucklandiae Radix, Inulae Radix, Vladimiriae Radix, and Aristolochiae Radix were collected in Korea and China. The ITS (Internal transcribed spacer) nucleotide sequences of samples were determined. The PCR primers to amply DNA marker of each herbal medicine were designed basing on the specific ITS regions showing differences in the sequences among medicinal plants. Results : Primer set Al R/IS F designed in this work amplified 220 bp PCR product only in samples of Aucklandiae Radix. In contrast, primer set Ih F/IS R, Vs R/IS F, and AcR F1/Ac R amplified 250 bp product, 356 bp prouct, and 516 bp product respectively to identify Inulae Radix, Vladimiriae Radix, and Aristolochiae Radix. Conclusions : The primers designed basing on the nucleotide sequences of ITS regions appearing differenced in the sequences among medicinal plants amplified the DNA markers for the identification of Aucklandiae Radix, Inulae Radix, Vladimiriae Radix, and Aristolochiae Radix. These herbal medicines were more efficiently identified by multiplex PCR method using all primers in a single PCR process.

• Analytical Science and Technology
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• v.34 no.6
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• pp.267-274
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• 2021

Acid-base titration experiments were carried out using the same sample to obtain a direct comparison of analysis results between a new titrator and conventional Spectrator (SPT) titrators. This allowed the confirmation of an endpoint both by spectroscopic detection and by conventional titrators (automatic burette (ABT) and autotitrator (ATT)). Statistical analysis was performed on the measured values (endpoints) obtained using different titrators, to assess their differences by comparison of straightness, equivalence, ANOVA, F-test, and t-test results. The coefficients of determination, confirmed by straightness, were all >0.999. Thus, the null hypothesis that there was no statistical difference could not be rejected. The Bland-Altman analysis method was used to confirm agreement with the new experimental method. No discrepancy was found in the measured values obtained using different titrators used in this study.

• Clinical Pain
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• v.20 no.2
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• pp.99-104
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• 2021

Objective: To confirm the safety of Intra-articular (IA) injection on the ipsilateral adhesive capsulitis (AC) after breast cancer surgery. Methods: Between January 2017 and May 2020, we retrospectively studied 29 patients after breast cancer surgery who underwent IA injection in the glenohumeral joint for AC in aseptic procedure. Results: There were no side effects or complications such as lymphedema or cellulitis in the patients. There was a significant improvement in pain score and range of motion (ROM) at the 1st, 3rd, and 6th months visits compared to the baseline (p<0.05). The presence or absence of axillary lymph node dissection and radiation therapy had no significant difference in improvement of ROM. But, in rotator cuff syndrome (RCS) group, there was a significant difference in improvement of shoulder IR in patients without RCS. Conclusion: IA Injection on the ipsilateral AC after breast cancer surgery was safe and even effective to improve pain and shoulder ROM. Ipsilateral IA injection can be a good treatment for breast cancer surgery patients suffering from AC.

• Proceedings of the Korea Water Resources Association Conference
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• 2021.06a
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• pp.394-394
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• 2021

이상기후로 인한 강우패턴의 변화는 상류 유역에서의 토양 유실, 비점오염물질의 발생을 가속화시켜 하류 수계의 수질 및 수생태 건강성에 악영향을 미치고 있다. 낙동강 수계에 위치한 내성천 유역에서는 토양 침투율이 높은 토양군으로 구성되어 있어, 강우 시 유출량 및 유사유출량의 비율이 높아 비점오염 저감을 위한 대책 수립의 중요성이 지속적으로 제기되어 왔다. 특히, 내성천 유역 내 상류에 위치한 토일천 및 낙화암천 소유역에서는 다양한 영농활동과 대규모 및 소규모 축사의 영향으로 강우 시 다양한 비점오염물질이 많이 발생하고 있다. 하류 하천에서의 수질을 효율적으로 개선하기 위해서는 비점오염 발생량이 높은 상류 소유역을 대상으로 적절한 최적관리기법 선정과 이에 대한 정량적인 평가 방법이 필요하다. 최근 식생여과대, 침사지 등과 같은 다양한 최적관리기법 중 인공습지에 대한 점오염원 및 비점오염물질 처리 효과가 국내·외 여러 모니터링 연구를 통해 증명되었다. 그러나 아직까지 유역 내 다양한 토양 및 토지이용상태와 그리고 오염원 유출 특성을 고려하여 인공습지의 조성에 따른 유역단위에서의 수질 개선 효과를 정량적으로 분석한 연구는 미비한 실정이다. 이에 본 연구에서는 장기 강우-유출 유역단위 모형인 SWAT(Soil and Water Assessment Tool) 내 인공습지 모의가 가능하도록 모형 소스코드를 수정하였으며, 내성천 상류 소유역(토일천, 낙화암천)에 위치한 인공습지 조성 전후에 따른 유역 말단에서의 유사 및 비점오염물질의 저감 효율을 비교 분석하였다. 향후 본 연구의 결과는 내성천 유역을 대상으로 인공습지를 통한 유사 및 비점오염 저감 대책 수립 시 기초자료로 활용될 수 있을 것이라 사료된다.

• Proceedings of the Korea Water Resources Association Conference
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• 2022.05a
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• pp.469-469
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• 2022

하구담수호는 하천의 종점에서 해양과 만나는 곳에 방조제를 건설해 담수를 유도하여 형성되는 인공호수이다. 유역 말단에 위치하기 때문에 유역에서 유출되는 모든 수자원을 확보할 수 있는 장점이 있지만, 유역에서 발생하는 오염물질이 모두 유입되고 인공적인 담수의 특성 때문에 수질이 악화되기 쉬운 조건을 지니고 있다. 담수호의 수질관리는 유입되는 오염물질의 양이 많고 담수된 수자원량도 많으므로 부분적인 방법으로는 개선이 어려우며, 유역과 호소를 포함한 종합적인 대책이 필요하다. 담수호의 수질관리대책 수립은 크게 상류 유역에 대한 대책과 호소에 대한 대책으로 구분된다. 대표적인 대책으로는 상류 유역의 하수처리장, 축사, 농경지 관리를 통한 배출부하량 감소와 호소 내 수질개선을 위한 습지, 저류지 건설 및 준설을 통한 내부부하 감소 등이 있다. 이처럼 담수호의 수질관리를 위해서는 상류 유역에서 호소까지 종합적으로 고려해야 한다. 본 연구에서는 유역모형과 호소모형의 연계를 통해 간월호 유역을 모의하였다. 유역모형은 HSPF(Hydrological Simulation Program-FORTRAN) 모형을 사용하였으며, 유역 내 3개의 하수처리장과 1개의 분뇨처리장을 고려하였으며, 4개 지점에 대하여 보정 및 검정을 시행하였다. 호소모형은 EFDC-WASP 연계모형을 이용하였으며, HSPF에서 모의 된 유입량과 호내 설치된 4개의 양수장, 배수갑문 운영일지를 고려하여 모의하였다. 호소 내 수질 측정지점에 대하여 T-N, T-P에 대하여 보정 및 검정을 수행하였다. 본 연구는 담수호 수질관리를 위한 분석시스템 구축으로 추후 대책에 따른 효과분석에 활용할 수 있을 것이다.

• Food Engineering Progress
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• v.15 no.1
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• pp.1-5
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• 2011

Codonopsis lanceolata L. (Campanulaceae) has long been used in traditional Korean medicine to treat bronchitis, cough, and inflammatory diseases, however, the efficacy of anti-tumor activities remains to be defined. In this study the effects of Codonopsis lanceolata (C. lanceolata) on proliferation, migration and adhesion in lung (A549, H1299) and ovarian cancer (SKOV-3) cells were investigated. To assess and compare the pharmacological effects and production places of C. lanceolata, the ethanolic extracts of C. lanceolata from different places in Korea (Hongseong, Yecheon, Yeongwol, Yanggu, Gangjin, and Hoengseong) were prepared. The extract from Hoengseong county did have only marginal anti-proliferative activity in all the cell lines tested, however, other extracts had little or no effect on cell proliferation. The extracts from Hongseong, Gangjin or Hoengseong county had partial anti-migratory activity in lung cancer cells, but not in ovarian cancer cells. In addition, the extract from Hoengseong county had partial anti-adhesive activity in ovarian cancer cells, however, other extracts did not affect cell adhesion in both lung and ovarian cancer cells. Taken together, these findings provide the first description of anti-tumor efficacy of C. lanceolata from different production places in Korea, and suggest that C. lanceolata from Hoengseong county may have therapeutic potential in lung and ovarian cancers.