• KOREAN JOURNAL OF CROP SCIENCE
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• v.28 no.2
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• pp.211-215
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• 1983

Thirty eight varieties with high uniformity and clearance in grain quality among 70 colored soybeans collected from nation-wide provinces were investigated for their seed morphological and agronomic traits. As for seed morphological traits, grain volumetric size, grain weight, grain length, grain thickness, . hilum length and width were studied on the tendency of statistical dispersion and variations. As a result, four groups could be classified on the basis of 100 grains weight, so as small, medium, large, and super large groups, Also, as for agronomic traits, among others, the tendency of setting in flowering date, maturing date, yield components, and stem weight, stem length were studied on the basis of statistical inter-relationships between above mentioned characteristics. From the above, bigger variations were detected in weighing characters of soybean plants than in duration characters. And the flowering and maturing days showed significant relationships to the stem length, stem weight, also stem length and stem weight to the grain yield.

• Journal of Mushroom
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• v.12 no.3
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• pp.226-231
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• 2014

In this study, SCAR marker that differentiates Pleurotus eryngii strains adaptable to high-temperature from control strain was developed. Genomic DNAs of 7 control strains of Pleurotus eryngii and 7 Pleurotus eryngii strains adaptable to high-temperature were analyzed by bulked segregant analysis (BSA) using randomly amplified polymorphic DNA (RAPD). Onehundred twenty RAPD primers were screened on bulked DNA samples and a unique DNA fragment with the size of 385 bp was yielded by OP-A06 primer from the Pleurotus eryngii strains adaptable to high-temperature. A sequence characterized amplified region (SCAR) marker, designated as OP-A06-1-F and OP-A06-1-R, was designed on the basis of the determined sequence. The PCR analysis with the OP-A06-1 primer showed that this SCAR marker can clearly distinguish the Pleurotus eryngii strains adaptable to high-temperature from the control strains.

• Korean Journal of Poultry Science
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• v.49 no.2
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• pp.99-108
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• 2022

Avian leukosis virus (ALV) is a highly contagious retrovirus that causes tumors and has resulted in great economic loss worldwide owing to its high transmission rate. Various ALV viral subgroups exist, with infections occurring via specific host receptors. The susceptibility or resistance of avian species to the ALV-A and K subgroups is determined by the host receptor, the tumor virus locus A (tva) gene, while that to ALV-B depends on another host receptor, the tumor virus locus B (tvb) gene. The resistance alleles of tva and tvb have primarily been identified in China, but none have beendetected in Korea. We analyzed the frequencies of tva and tvb genotypes in White Leghorn (WL), Korean Ogye (KO), and Korean native chicken (KNC) breeds, and assessed the resistance to ALV subgroups. In WL, both tva and tvb had various genotypes, including susceptibility and resistance alleles, whereas in KO, tva and tvb resistance alleles were dominant. In KNC, tva susceptibility and resistance alleles were mixed, whereas tvb resistance alleles were dominant. In addition, we showed that there were differences in the splicing pattern of tva transcripts and the expression level of tvb transcripts within breeds. Finally, we confirmed that ALV resistance depended on KO and KNC genotypes by in vitro infection of chicken embryonic fibroblasts with ALV. These results highlight that some KO and KNC individuals are naturally resistant to ALV subgroups A, B, and K, and will facilitate the preservation of economically superior traits through selective breeding.

• Journal of Forest and Environmental Science
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• v.11 no.1
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• pp.81-101
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• 1995

Random amplified polymorphic DNA (RAPD) technology, a recent approach in molecular genetics, is much usable to select the elite trees and to maximize the genetic gain in forest tree breeding program, providing a clue to determine the genetic marker-trait correlation. This review intorduces research on bark thickness and breeding strategy in Pinus elliottii, Pinus caribaea and their hybrid by Queensland Forest Service and ForBio Research Pty Ltd, University of Queensland, which employ RAPD technology. Genetic linkage map of $F_1$ hybrids includes 186 RAPD markers and 16 linkage groups (1641 cM long in total) and 6 quantitative trait loci are located putatively for bark thickness. Following recent research results and experiences in pine breeding programs, the forseeable stages in the application and development are proposed for marker assisted selectin; stage 1-determination of species specific markers for genes controlling traits of commercial interest, and stage 2-determination of marker-allele association for specific allelic variants within pure species. As pines inherit their megagametophytes from the seed parent and zygotic embryos from both male and female parents, the determination of marker-trait correlation is possible even in embryo stage, eventually making ways for the early selection of elite individuals.

• Korean Journal of Agricultural Science
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• v.13 no.1
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• pp.17-32
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• 1986

To obtain basic information needed for developing better pearl barley varieties, a total of 148 lines of pearl barley were collected from nationwide survey except for Kangwon and Chejoo provinces and classified by principal component analysis. The results are summarized as follows : 1. Variabilities of characters for all lines except for leaf width and 100 K. Wt.(Unpolished) were high enough to indicate variation of lines. 2. Correlation coefficients among 18 characters were high enough and they showed the shape of normal distribution, more or less, inclined toward positive values. 3. The lines could be classified into four groups by correlation coefficient for 18 characters : Group I was characterized as the lines composed of grain and plant type, Group II maturity, Group III the number of tillers, and Group IV the nature of germination, respectively. 4. About 60% of the total variation could be appreciated by the first four principal components and about 89% of the total variation by the first ten principal components. 5. Contribution of characters to principal components was variable and was high at upper principal components and low at lower principal components. 6. The value of eigen vector corresponding to those which had high significant correlation coefficient between characters was almost of the same value. 7. The lines were classified into four groups by principal component analysis. 8. The lines were also classified into four groups by taxonomic distance. Group I included 79 lines, Group II 40 lines, Group III 22 lines, and Group IV 7 lines, respectively. 9. Four groups classified by taxonomic distance could be characterized as follow : Group I : medium height plant, small kernels, medium maturity, and narrow and short leaf, Group II : short height plant, small kernels, early maturity, and narrow and short leaf. Group III : tall height plant, large kernels, late maturity, and broad and long leaf. Group IV : short height plant, large kernels, medium maturity, and narrow and short leaf.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.18
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• pp.124-155
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• 1975

To obtain the fundamental informations on the varietal improvement of peanut and to study the ecological variations of the important agronomic traits and to the relationship between the traits studied, an investigation was made on varietal classification of 489 introduced on the basis of their morphological and ecological differences at Crop Experiment Station, Suweon in 1968, and the other study conducted at some location as above in 1969 was to investigate the ecological variations of the materials in accordance with changes of seeding date using classified varietal group under 5 different seeding times from April 16 to July 7 with twenty days interval. The results obtained were summarized as follows: 1. Peanut varieties tested were classified into Spanish, Virginia Erect, Virginia Runner, Southeast Runner. Valencia and Semirunner, on the basis of plant type, flowering time, number of grains per pod and grain size. 2. Characteristics of varietal group classified are as followings. (1) Spanish; erect, small grained and early maturing type. (2) Virginia Erect; erect, large grained and late flowering type. (3) Virginia Runner; runner, large grained and late maturing type. (4) Southeast Runner; runner, small grained and early maturing type. (5) Valencia; erect, small grained and early flowering type with 3-4 grains per pod. (6) Semi runner; semirunner, large grained and late flowering type. 3. Flowering period in respective varietal group was consistently shorted by delayed seeding date and the degree of shortening was more serious in late flowering varietal group. 4. Number of branches per plant was generally decreased in late seeding date in respective group. However, Spanish and Virginia Runner exhibited lower number of branches in the first seeding rather than the second seeding and the lowest number of branches was found in Spanish while the highest were Virginia Erect in all seeding date. 5. Shelling ratio was high in Spanish and Southeast Runner in any seeding date and decreased remarkedly by seeding after May. 6. Number of pod per plant in all varietal groups was remarkedly decreased by delayed seeding date and the degree of decreasing was more serious in large grain varietal group. 7. The higher pod weight per plant was found in second seeding date rather than first seeding and pod weight per plant was decreased obviously in all late seeding after the second. Therefore, among the cultivars tested, Southeast Runner noted the highest pod weight per plant while Virginia Runner showed the lowest. 8. Grain number per plant expressed the similar tendency as the pod weight per plant but was low in large grain group and high in small grain group in all seeding date employed. 9. 100 grain weight was heaviest in second seeding and was decreased remarkedly after the second and even the first seeding date. 10. Yield per 10a noted considerable variations in accordance with seeding date in all groups classified. However, the yield was increased in second seeding date (May 7) and decreased in the others. 11. Length of main stem and branches were exceptionally decreased in the first seeding date compare to the second in Spanish while other varieties were tend to be same between the indicated seeding date, but. these two traits were strikingly decreased in all seeding after the second. This tendency, however, strongly suggested the importance of environmental effects on peanut growth in terms of their changes due to the different seeding date. 12. Highly significant positive correlations were showed between yield and yield componets such as pod weight per plant, 100 grain weight and the number of grains per plant in all varietal groups except, Virginia Runner. However, the other characters were almost not correlated with yield and differences in correlation coefficients among the seeding dates were found. 13. Path coefficients estimated for yield components to yield was higher in number of grains per plant pod weight per plant and 100 grain weight in terms of direct effect and the other components were negligible in all varietal groups. 14. Heritabilities estimated were generally high in pod number per plant, shelling ratio, 100 grain weights and number of grains per pod and the other traits were relatively low.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.12a
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• pp.51-51
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• 2020

콩의 원산지는 만주를 중심으로한 동북아시아를 원산지로 추정하고 있으며, 동아시아인 한국, 중국, 일본의 주요 식재료로 다양하게 이용되어왔다. 콩은 단백질과 지방의 공급원으로서 뿐 아니라 최근에는 콩의 생리활성 물질이 항암효과, 골다공증, 신장질환과 같은 질병예방에도 효과가 있는 것으로 알려지면서 활용가능성이 높아지고 있다. 본 연구는 유전자원센터에 보존 중인 콩 수집단에서 농업형질, 기초정보 등을 토대로 핵심집단을 작성하고 그 중 한국, 중국, 일본 수집단 각 425, 93, 42자원의 개화일수, 생육일수, 주당협수, 100립중, 주당수량과 같은 양적형질을 비교하였다. 개화일수와 생육일수는 중국수집 자원에서 평균38일, 107일로 가장 빠르며, 한국수집 자원은 평균 53일, 127일로 가장 늦었다. 협당립수는 중국수집 자원에서 협당 평균3립으로 가장 많았으며, 한국과 일본 수집자원은 각 2.3과 2.4립으로 유사하였다. 주당협수는 한국 수집자원에서 주당 평균 327.4개로 가장 많고 일본이 248.0개 중국이 224.4순으로 조사되었다. 100립중은 일본 수집 자원이 평균 33.6g으로 대립이 많고 한국 26.5g , 중국 21.1g 이었다. 주당수량은 한국 수집자원이 평균 119.8g, 일본 84.2g, 중국 76.8g순이었다. 이들 5개 양적형질을 토대로 군집분석한 결과 한국 자원은 대다수가 C3 그룹에 속하고, C1 그룹에 일부가 속하였으며, 일본 자원은 C3 그룹과 C2 그룹에, 중국 자원은 C2 그룹에 속하여 한국과 일본자원이 유사하고 중국자원과는 차이를 보이는 것으로 나타났다. 이러한 특성조사 결과는 자원의 수집, 도입시 다양성 확보를 위한 기초정보로 이용할 수 있다.

• Korean Journal of Animal Reproduction
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• v.25 no.4
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• pp.295-304
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• 2001

Characterization of quantitative trait loci (QTL) was investigated in the experimental crosses between Berkshire and Yorkshire breed. A total of 525 F$_2$ progenies from 65 matting of F$_1$ Parents were produced. Phenotypic measurements included average daily gain (ADG), average back fat thickness (ABF), and loin eye area (LEA). To identify the presence of QTL for reproductive performance, birth weight (BWT) and body weight at 16 days (16DAY) were included as indirect trait. QTL segregation was deduced using 8 markers assigned to chromosome 2 (SSC2). Quantitative trait locus analyses were performed using interval mapping by regression under line-cross model. Presence of imprinting was tested under the statistical model that separated the expression of paternally and maternally inherited alleles. To set the evidence of QTL presence, significance thresholds were derived by permutation following statistical tests, respectively. Genome scan revealed significant evidence for three quantitative trait loci (QTL) affecting growth and body compositions, of which two were identified to be QTL with imprinting expression mode near the ICF II gene region. For average back fat thickness (ABF), a paternally expressed QTL was found on chromosome 2 (SSC2). A paternally expressed QTL affecting loin eye area (LEA) was found in the region of SSC2 where evidence of imprinted QTL was found for average back fat thickness (ABF). For average daily gain (ADG), QTL expressed with Mendelian mode was found on chromosome 2 (SS2). Also, QTL affecting average daily gain (ADC), was identified to be expressed with Mendelian express mode.

• Journal of Animal Science and Technology
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• v.49 no.4
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• pp.437-442
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• 2007

The melanocortin-4 receptor(MC4R) is virtually expressed in all brain regions and plays an important role in energy homeostasis in mammals. MC4R has been intensively studied as a trait gene controlling economically important traits, such as growth and feed conversion, etc. Six hundreds and sixty Duroc pigs were genotyped for the MC4R locus and analyzed their relationships with breeding values for average daily gain(ADG), backfat thickness(BF), days to 90kg(D90) and feed conversion(FC). The estimated genotype frequencies for the all Duroc pigs were: 30.8%, 45.2%, 24.1% for AA, AB and BB genotypes, respectively. The mutant A allele was significantly associated with ADG, D90 and BF whereas no significant relationship was found with FC. The change of gene frequencies by generation was shown in both selected and culled groups. These results indicate that the MC4R polymorphism could be integrated in the present selection program to realize a marker-assisted selection for the growth traits of the Duroc population.

• Journal of Periodontal and Implant Science
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• v.36 no.1
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• pp.113-123
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• 2006

연구배경 IgG에 대한 $Fc{\gamma}$ 수용기는 치주병인균에 대한 숙주 반응에 있어서 중요한 역할을 하는데, 이 중 $Fc{\gamma}RIIIa$는 NK 세포, 대식세포, 단핵구, ${\gamma}{\delta}T$세포에서 발현되며, EC2 도메인에서 158 아미노산 부위의 valine (V)-phenylalanine (F)의 유전자다형성을 보인다. $Fc{\gamma}RIIIb$는 특이적으로 중성구에 발현되는데, extracellular (ECl) Ig-like 도메인 내 4개의 아미노산 치환(substitutions)에 의한 NA1-NA2 유전자다형성을 보인다. 이 연구의 목적은 한국인에서 급진성 치주염 환자와 $Fc{\gamma}III$ 수용기의 유전자다형성과의 관련성을 알아보는 것이다. 연구방법 및 재료 치주적으로 건강한 90명 (대조군, 남자 64명, 여자 26명)과 서울대학교 치과병원 지주과에 내원하여 급진성 치주염으로 진단된 환자 43명 (aggressive periodontitis patients: AgP, 남자 30명, 여자 13명)을 대상으로 하였다. 모든 실험 대상자는 임상 실험에 대해 동의 하였고, 초진 시 전자 탐침(Florida Probe(R) Co. Gainesville, FL)을 이용하여 탐침 시 치주낭 깊이 (PPD), 임상부착수준 (CAL), 치태지수(PI), 탐침 후 출혈지수 (BOP)를 측정하였다. 또한 이들의 정맥혈에서 추출한 DNA를 PCR법, 전기영동법 등을 이용하여 $Fc{\gamma}RIIIa$, $Fc{\gamma}RIIIb$의 대립 유전자의 존재여부를 확인하였다. 이를 바탕으로 $Fc{\gamma}RIII$ 복합 유전형을 확인하여 각 군 간을 비교하였다. 연구 결과 1. $Fc{\gamma}RIIIa$에 대한 유전자다형성 연구 결과 대조군과 급진성 치주염 환자 군(AgP)사이에서는 대립 유전자 분포가 서로 유의성 있는 차이를 나타내었고 (P<0.05), $Fc{\gamma}RIIIb$에서는 유의성 있는 차이를 발견할 수 없었다. (P>0.05) 2. $Fc{\gamma}RIIIa$ 158F 대립형질이 급진성 치주염 환자에서 유의성 있게 많이 발견되어졌다. (P<0.05) 결론 이 연구를 통하여 $Fc{\gamma}RIIIa$ 유전자의 분석이 한국인의 급진성 치주염에 대한 감수성의 위험요소의 표지자로 활용할 수 있을 것으로 사료되며, 향 후 더 많은 환자를 대상으로 히는 추가 연구가 필요하다고 생각된다.