• Journal of the Korean earth science society
• /
• v.27 no.1
• /
• pp.95-117
• /
• 2006

From the Tertiary Yeonil Group distributed in Songra and Cheongha areas, the northern part of the Pohang Basin, nine archaeomonad species belonging to two genera, and other three types of chryophycean cysts considered as stomatocysts, five endoskeletal dinoflagellate species belonging to three genera and eighteen ebridian species belonging to eleven genera were identified. Based on above siliceous microfossil assemblages, the Yeonil Group is corresponded to Middle Miocene age. The group is correlated with the Calvert Formation (Maryland in USA) and the Hojuji Formation (in Central Japan) by its archaeomonad cysts. And, the group is correlated with the intervals of Actiniscus elongatus to Middle Hermesinella conata zones in Southwest Pacific region and of Spongebria miocenica to Middle Hermesineila schulzii zones in $V{\o}ring$ Plateau, Norwegian Sea, based on the ebridian assemblages of the group. From the chrysophycean cyst including archaeomonad, endoskeletal dinoflagellate and ebridian assemblages in the Yeonil Group of the study area, it is inferred that cold water masses dominated during the deposition oi the group with occasional warm water. The upper part of the group might be somewhat colder than the lower one of the group in depositional condition. In addition, minute chrysopycean cysts considered as stomatocysts suggest the influence of fresh or brackish water during the deposition of the group.

• Korean Journal of Ecology and Environment
• /
• v.38 no.4 s.114
• /
• pp.475-481
• /
• 2005

In an effort to identify a new environment-friendly algicide, we examined the ability of extracts from the leaves and stems of nine Korean oak tree species to inhibit growth of the bloom-forming cyanobacterium, Microcystis aeruginosa. At a concentration of 100 mg $L^{-1}$, five of the oak tree extracts (QAT-L, QAT-5, QAS- L, QGI-5, and QSA- L) decreased the cell density of M. aeruginosa by over 90% for 7 days. At a concentration of 20 mg $L^{-1}$, the same five extracts inhibited the growth of M. aeruginosa by approximately 50%. The minimum concentration of oak tree extracts required for effective inhibition of M. aeruginosa (20 mg $L^{-1}$) is comparable to that of the known algicide, tannic acid (17 mg $L^{-1}$), which is thought to be one of the main active ingredients in the oak tree extract. These findings suggest that oak extracts may be useful as an environment-friendly algicide to control the bloomforming cyanobacterium, M. aeruginosa, in eutrophic waters.

• Ocean and Polar Research
• /
• v.27 no.2
• /
• pp.149-159
• /
• 2005

Samples were collected from five stations in February, May, July, and September 2004 to investigate seasonal variations in the phytoplankton community and the relationship between dominant genera and environmental factors in Asan Bay. In February, microphytoplankton contributed 80% to the total chlorophyll a. Diatom dominated the phytoplankton community, accounting for 85.9% of the total cell number, followed by dinoflagellates (6%). Dominant species were Skeletonema costatum and Thalassiosira spp. Abundant diatom, including S. costatum and Thalassiosira spp., may be affected by water temperature and silicate at Station 1 and 2 in February 2004. In May, the nanophytoplankton contribution to total phytoplankton was higher than in other seasons. However, abundance of S. costatum and Thalassiosira spp. decreased, since the growth of S. costatum and Thalassiosira spp. might be limited by phosphates (P) resulting from low P concentration and a high DIN:DIP ratio in the outer region. In July, dominant phytoplankton groups were diatom (39%), cryptophyceae (28%), and cyanophyceae (20%). Dominant genera were Oscillatoria spp. and phytoflagellate of a monad type in the inner region (Station 1 and 2), whereas S. costatum was dominant in the outer region (Station 4 and 5). In September, dominant phytoplankton were diatom (69%) and cryptophyceae (28%). Dominant genera were phytoflagellate of the monad type, S. costatum in the inner region, while Chaetoceros spp. was dominant in the outer region.

• Microbiology and Biotechnology Letters
• /
• v.26 no.1
• /
• pp.1-6
• /
• 1998

The characteristics of $CO_2$ fixation by Chlorella HA-1 cultured in bubble columns were studied to achieve high photosynthetic rates per basal area. The influence of experimental conditions such as the diameter of a bubble column and gas flow rate, on photosynthesis of Chlorella HA-1 was investigated. The maximum productivity and the overall $CO_2$ fixation rate obtained in a 0.15 L bubble column was 1.09 g dry biomassa-day and 1048 g CO$_2/\m^2$-day, respectively. Light limitation has been observed in the bubble columns having a diameter larger than 3.5 cm.. As the reactor volume increased, the decrease of the $CO_2$ fixation rate was remarkable. High gas flow rate was helpful to mitigate the light limitation problem.

• Journal of the Korean Society of Marine Environment & Safety
• /
• v.27 no.2
• /
• pp.340-354
• /
• 2021

The aim of this study was to investigate the spatial and temporal distribution of phytoplankton biomass and community composition in Jinhae Bay on the southern coast of Korea. Phytoplankton pigment analysis was conducted using ultra performance liquid chromatography (UPLC) were conducted from April to December 2019 at seven stations. Temperature, salinity, and dissolved oxygen (DO) and inorganic nutrients (dissolved nitrogen, dissolved phosphorus, and orthosilicic acid) were measured to investigate the environmental factors associated with the structure of phytoplankton community. Phytoplankton biomass (Chl-a) was the highest in July (mean 15.4±4.3 ㎍/L) and the lowest in December (mean 3.5±0.6 ㎍/L). Fucoxanthin was the most abundant carotenoid and showed a similar variation pattern to Chl-a, peridinin, and Chl-b. Phytoplankton community composition analysis showed that diatoms were a predominant group with an average abundance of 70 % whereas chlorophytes, cryptophytes, and dinoflagellates often appeared with lower averages. Further, the dominance of diatoms was closely correlated with water temperature and N:P ratio, which might be influenced by high temperatures in the summer and nutrient loading from the land. Additionally, freshwater and nutrient input by rainfall was estimated to be the most important environmental factor. Hence, the spatial and temporal variations in the composition of phytoplankton pigments and phytoplankton community were correlated with physicochemical and environmental parameters.

• Journal of Korean Society of Water and Wastewater
• /
• v.18 no.6
• /
• pp.794-800
• /
• 2004

The purpose of this study is to propose a method of controlling freshwater algae which grows abundantly and forms water bloom in the eutrophic water body with $H_2O_2$. Both laboratory and field methodologies were used. For the laboratory test $H_2O_2$ was injected into the different growth phases of incubated Microcystis aeruginosa and the resulting algae growth control rate was examined. For the field test, $H_2O_2$ was dispersed into a lake. Lake water quality was evaluated using a pre-test and post-test analysis of chlorophyll-a, luminance, transmittance, etc., which allowed a comparative evaluation of water quality change. From the experimental results, the growth of algae can be controlled with the small amount of 1mg/L of $H_2O_2$ at the lag phase of growth. The field test results show that the green colour of lake water was removed completely by the reduction of chlorophyll-a and improved transmittance, luminance, TKN, TP, TOC and SS. These indicators of water quality were improved significantly after $H_2O_2$ injection. Toxicity test results using the lake fish show no evidence of detrimental effect of $H_2O_2$ up to 15mg/L. The results of $EC_{50}$ with P. phosphoreum show that the toxicity of $H_2O_2$ was negligible compared to copper which was commonly used for algae control.

• Proceedings of the Korea Water Resources Association Conference
• /
• 2016.05a
• /
• pp.358-358
• /
• 2016

본 연구에 앞서 가뭄에 따른 충청남도 서부권의 물 부족을 해결하기 위하여 금강 본류로부터 보령댐으로 일정 유량을 도수(diversion)하는 계획이 금강 본류에 미치는 수리적 영향을 1차원 비정상류 해석(unsteady flow analysis)을 통하여 분석하였으며, 본 연구에서는 수리모형 결과와 연계하여 수질 모형을 구축하여 금강 본류 수질에 미치는 영향을 알아보고자 하였다. 조위 영향에 따른 염도의 역류 모의를 위하여 전자해도와 위성영상자료를 활용하여 금강 하굿둑에서 외해 방향으로 약 9km에 이르는 모의 구간을 확장하였으며, 상류단의 진두수위표를 포함한 8개 지천의 유입유량 및 수질, 하구의 조위와 염도, 농공용수 취수시설의 취수량 및 회귀수 수질, 금강 배수갑문 운영, 대상 구간에 인접한 기상대의 기상자료 등을 고려하고 금강 본류를 따라 9개 수질측정소의 수질자료를 이용하여 모형의 보정을 실시하였다. 또한 대상 구간 내 2015년의 갈수기 유황 분석을 토대로 비선형 회귀분석식을 도출하여 2016년 갈수기 예측 유량과 함께 수질-유량 관계식을 도출하여 대상 구간의 수온, 염도, 용존산소(DO), BOD, 조류 등 영향을 분석하였다. 수온 분석 결과, 지점에 따라 2015년 유황 대비 평균 수온 1.79~3.83도, 최고 수온 1.74~4.30도 상승할 것으로 모의되었으며, 염도는 2016년 갈수기 동안의 금강 하굿둑 외해 염도는 담수 방류량의 감소로 전년 동기 대비 평균 53% 증가하는 것으로 모의되어 기수역 생태계에 상당한 영향을 줄 것으로 분석되었으며, 갈수기 동안의 금강 하굿둑 내수의 염도는 배수갑문 개방 빈도, 개방 시간, 개방 정도의 감소에 따른 해수 역류의 감소로 전년 동기 대비 크게 감소할 것으로 모의되었다. 용존산소는 2016년 갈수기 동안의 용존산소 농도는 지점에 따라 전년 동기 대비 평균 4~11% 감소하는 것으로 모의되었으며, 2016년 갈수기 동안의 BOD 농도는 지점에 따라 전년 동기 대비 평균 30~57% 증가하는 것으로 분석되었다. 조류는 지점에 따라 전년 동기 대비 평균 29~67% 증가하는 것으로 모의되었다. 유량의 감소에 따른 수온 증가 및 수질 악화의 영향이 있을 것으로 분석되나, 도수에 따른 영향은 상대적으로 제한적인 것으로 분석되었다.

• Journal of Life Science
• /
• v.32 no.8
• /
• pp.659-665
• /
• 2022

Chlorella vulgaris is an important freshwater alga that is widely used as a food source by humans and animals. Recently, Chlorella has received considerable attention with regard to its potential application in aquaculture and the production of biofuels, nutrients, and therapeutic proteins. Recently, our laboratory acquired a new strain of C. vulgaris, PKVL7422, characterized by fast growth, ease of culture, and cultivability under dark conditions. However, the genes involved in its nitrogen assimilation are unknown. In this work, we identified the nitrate reductase (NR) gene of C. vulgaris PKVL7422 using rapid amplification of cDNA ends and genome walking. The NR gene of C. vulgaris PKVL7422 is approximately 8 kb long and composed of 18 introns and 19 exons, which encode 877 amino acids. An alignment analysis of the NR gene showed that it possesses the five domains and several invariant residues found in plant NRs. These results provide new insight into the molecular organization of the NR gene in algae.

• Proceedings of the Korea Water Resources Association Conference
• /
• 2023.05a
• /
• pp.234-234
• /
• 2023

유해 남조 대발생(Harmful Algal blooms, HABs)이 담수호에 발생하면 마이크로시스틴과 같은 독성물질과 맛·냄새 물질을 생성하여 상수원이용과 친수활동을 방해한다. 그래서 유해 남조 대발생 전 유해남조 세포수를 예측하여 선제적 대응하는 것은 중요하다. 따라서 본 연구는 머신러닝기반 Random Forest(RF)를 활용하여 팔당댐 앞의 유해남조 세포수를 예측하는 모델을 개발하고 성능을 평가하고자 한다. 모델 구축을 위해 2012년 4월부터 2021년 12월까지의 팔당호(삼봉리, 경안천) 및 남북한강(의암댐~이포보)권역의 조류, 수질, 수리/수문, 기상 자료를 수집하여 입력 및 출력 자료로 이용하였다. 수집된 데이터에는 다양한 입력변수들이 있어 남조 세포수 예측 성능 비교를 위한 전체 26개 변수 적용과 통계학적으로 상관관계가 높은 12개 변수 적용을 통해 모델을 구축하였다. 입력, 출력 자료로 이용한 유해남조 세포수는 로그변환된 값으로 사용하였으며 일반적인 조류 시료 채취기간이 7일이므로 7일 후를 예측하기 위한 모델을 구축하였다. 구축한 모델의 성능은 실측데이터와 예측데이터의 R²로 산출하여 평가하였다. 전체 26개 입력변수로 모델 구축 후 학습 및 검증 수행 결과 R²의 학습 0.803, 검증 0.729로 나타났고, 유해남조 세포수와 유의미한 상관관계를 보이는 12개 입력변수로 모델 구축 후 학습 및 검증 수행 R²은 학습 0.784, 검증 0.731로 나타났다. 두 모델의 성능을 살펴본 결과 입력변수 개수의 변화에 따른 성능차이는 크지 않은 것으로 나타났으며, 남조세포수 예측을 위한 모델로서 활용가능함을 알 수 있었다. 향후 연구에서는 Random Forest 외 다른 기계학습 모델들과 딥러닝 모델을 통해 남조세포수 예측 성능이 높은 모델을 구축해볼 필요성이 있다.

• The Korean Journal of Pesticide Science
• /
• v.12 no.1
• /
• pp.50-56
• /
• 2008

The purpose of the study was to determine the effects of isoprothiolane, diazinon, butachlor, dimethametryn and molinate in Selenastrum capriconurtum, Scenedesmus subspicatus, Chlorella vufgaris and Nitzschia palea during an exposure period of 72 hours. The study was carried out in according with the OECD Guidelines for Testing of Chemicals No 201 Alga, Growth Inhibition Test. The toxicological responses of isoprothiolane to S. capriconutum, S. subspicatus, C. vulgaris and N. palea expressed as in individual $ErC_{50}$ (Median Effective Concentration by growth rate) value, were 5.87, 9.91, $18.55 and 38.79\;mg\;L^{-1}$, respectively. Diazinone to S. capriconutum, S. subspicatus, C. vulgaris and palea expressed as in individual $ErC_{50}$ value, were 10.31, 11.44, >32 and $14.32\;mg\;L^{-1}$, respectively. Butachlor to S. capriconutum, S. subspicatus, C. vulgaris and N. plaea expressed as in individual $ErC_{50}$ value, were 0.002, 0.019, 8.67 and $4.94\;mg\;L^{-1}$, respectively. Dimethamelryn to S. subspicatus, C. vulgaris and N. palea expressed as in individual $ErC_{50}$ value, were 0.0071, 0.011, 0.0065 and $0.009\;mg\;L^{-1}$, respectively. Molinate to S. capriconutum, S. subspicatus, C. vulgaris and N. palea expressed as in individual $ErC_{50}$ value, were 0.44, 1.26, 48.84 and $28.52\;mg\;L^{-1}$, respectively. The sensitivities of five pesticides were different depending on freshwater alga in the order of S. capriconutum > S. subspicatus > C. vulgaris, N. palea. Highly significant correlation (r=0.9677) based on $ErC_{50}$ were found between S. capriconutum and S. subspicatus.