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Tandem Mass Spectrometry of N-linked Glycans from Human Immunoglobulin G (다중 질량 분석법을 이용한 인체 면역글로불린 G의 N-연결 글라이칸 분석)

  • Joo, Hwang-Soo;Kim, Yun-Gon;Jang, Kyoung-Soon;Kim, Byung-Gee
    • KSBB Journal
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    • v.22 no.4
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    • pp.234-238
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    • 2007
  • We used electrospary ionization ion trap tandem mass spectrometry (ESI-IT tandem MS) to structural elucidation of three different biantennary-type glycans having zero, one, two galactoses (G0, G1, G2). The highest fragment ion in the MS/MS spectra of three glycans was produced by 0,2-ring cleavage of fucose-linked N-acetylglucosamine (GlcNAc) in reducing end. The fragment ions both from precursor ions and 0,2-ring cleaved ions ($^{0.2}An$; n=5 for G0, n=6 for G1 and G2) were not overlapped each other. As results of $MS^n$ analyses, tandem fragmentation trees of each glycans were generated and 2,4-ring cleavages ($^{2.4}A_6$) were occurred in GlcNAc linked to reducing end GlcNAc. This structural elucidation and fragmentation study of N-linked glycans by tandem mass spectrometry can be applied to structural analysis of more complicated glycans.

Preliminary Construction Cost Prediction Model Based on Module for Modernized Hanok (초기 기획단계의 신한옥 공사비 예측 모델 - 모듈(칸) 기반의 목공사 개략 물량 산출 중심으로 -)

  • Kang, Seunghee;Jung, Youngsoo
    • Korean Journal of Construction Engineering and Management
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    • v.21 no.3
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    • pp.48-56
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    • 2020
  • Prediction of construction cost in the planning stage that provides basic information for feasibility study, budgeting, and planning is an important factor for successful project execution. In this study, a prediction model was developed for the purpose of improving the accuracy of estimating the construction cost of Hanok in the planning stage. The cost of this model is estimated by two methods. First, the cost of wood work, which accounts for the largest portion of the total construction cost, is estimated by calculating the approximate quantity under various conditions (structure type, roof type, plane type, etc.). Second, the cost of the rest work sections except the wood work is estimated by using the unit cost model. The predictive model was verified by two case projects, and the error rate of total construction cost was -4%(case 1) and -6%(case 2). These results showed an error rate in the range that can be applied to practice in the planning stage.

Discrimination of African Yams Containing High Functional Compounds Using FT-IR Fingerprinting Combined by Multivariate Analysis and Quantitative Prediction of Functional Compounds by PLS Regression Modeling (FT-IR 스펙트럼 데이터의 다변량 통계분석을 이용한 고기능성 아프리칸 얌 식별 및 기능성 성분 함량 예측 모델링)

  • Song, Seung Yeob;Jie, Eun Yee;Ahn, Myung Suk;Kim, Dong Jin;Kim, In Jung;Kim, Suk Weon
    • Horticultural Science & Technology
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    • v.32 no.1
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    • pp.105-114
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    • 2014
  • We established a high throughput screening system of African yam tuber lines which contain high contents of total carotenoids, flavonoids, and phenolic compounds using ultraviolet-visible (UV-VIS) spectroscopy and Fourier transform infrared (FT-IR) spectroscopy in combination with multivariate analysis. The total carotenoids contents from 62 African yam tubers varied from 0.01 to $0.91{\mu}g{\cdot}g^{-1}$ dry weight (wt). The total flavonoids and phenolic compounds also varied from 12.9 to $229{\mu}g{\cdot}g^{-1}$ and from 0.29 to $5.2mg{\cdot}g^{-1}$dry wt. FT-IR spectra confirmed typical spectral differences between the frequency regions of 1,700-1,500, 1,500-1,300 and $1,100-950cm^{-1}$, respectively. These spectral regions were reflecting the quantitative and qualitative variations of amide I, II from amino acids and proteins ($1,700-1,500cm^{-1}$), phosphodiester groups from nucleic acid and phospholipid ($1,500-1,300cm^{-1}$) and carbohydrate compounds ($1,100-950cm^{-1}$). Principal component analysis (PCA) and subsequent partial least square-discriminant analysis (PLS-DA) were able to discriminate the 62 African yam tuber lines into three separate clusters corresponding to their taxonomic relationship. The quantitative prediction modeling of total carotenoids, flavonoids, and phenolic compounds from African yam tuber lines were established using partial least square regression algorithm from FT-IR spectra. The regression coefficients ($R^2$) between predicted values and estimated values of total carotenoids, flavonoids and phenolic compounds were 0.83, 0.86, and 0.72, respectively. These results showed that quantitative predictions of total carotenoids, flavonoids, and phenolic compounds were possible from FT-IR spectra of African yam tuber lines with higher accuracy. Therefore we suggested that quantitative prediction system established in this study could be applied as a rapid selection tool for high yielding African yam lines.

Two-sample chi-square test for randomly censored data (임의로 관측중단된 두 표본 자료에 대한 카이제곱 검정방법)

  • 김주한;김정란
    • The Korean Journal of Applied Statistics
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    • v.8 no.2
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    • pp.109-119
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    • 1995
  • A two sample chi-square test is introduced for testing the equality of the distributions of two populations when observations are subject to random censorship. The statistic is appropriate in testing problems where a two-sided alternative is of interest. Under the null hypothesis, the asymptotic distribution of the statistic is a chi-square distribution. We obtain two types of chi-square statistics ; one as a nonnegative definite quadratic form in difference of observed cell probabilities based on the product-limit estimators, the other one as a summation form. Data pertaining to a cancer chemotheray experiment are examined with these statistics.

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Adaptive Searching Estimation in Stratified Spatial Sample design (적합탐색 관찰을 이용한 층화 공간표본설계에서의 추정)

  • 변종석
    • The Korean Journal of Applied Statistics
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    • v.13 no.2
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    • pp.353-369
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    • 2000
  • We systematized an stratified spatial sample design(SSSD) that uses the adequate stratification criteria such as the shapeness or the dispersion of an interesting region in a spatial population. And we proposed an adaptive searching estimation method in the SSSD to estimate the area of region of interest in two-dimensional surfaces. When wc adopt the proposed adaptive searching estimation method in SSSD, the observing sample size is more decreased than a classical sample design that all the designed sample size is observed. Nevertheless it has been shown that we can produce the moderate result but the efficiency is a slight reduced.

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A Study Building of Ssang-Bong Sa the Main Temple - Focused on Structure and Design of Pagoda Body - (쌍봉사 대웅전의 조영에 관한 고찰 - 탑신부(塔身部)의 구조와 의장을 중심으로 -)

  • Yang, Tae-Hyeon;Cheon, Deuk-Youm;Lee, Jae-Yeoun
    • Journal of architectural history
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    • v.22 no.1
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    • pp.41-50
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    • 2013
  • In Korea, only a few wooden pagodas are extant because some wooden pagodas were lost due to artificial environment like war. Fortunately, only Eight Depictions Hall(Palsangjeon) in Beobjusa temple and main hall of Ssang-Bong Sa the main temple are extant. Though main hall of Ssang-Bong Sa the main temple shows old style in construction and outstanding creativity, survey and investigation for the hall have been poor. Accordingly, this study investigated pillar part, bracket structure part, and roof part composing pagoda body section which actively reflects structure and design skill compared to floor or upper part. And for better understanding, in the part that is similar to pagoda body section of main hall or needs examples, wooden pagoda in China or Japan was referred. Through this investigation, it was known that unique skill applied to main hall of Ssang-Bong Sa the main temple is based on plane in one room ${\times}$ one room - Ssang-Bong Sa the main temple has common form of wooden pagoda in appearance.

A Study on the Basic Planning of the Nam-Hae Sin-Sa Architecture (남해신사 기본계획에 따른 신당건축 고찰)

  • Kim, Sang Tae;Jang, Hun Duc
    • Korean Journal of Heritage: History & Science
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    • v.42 no.2
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    • pp.62-85
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    • 2009
  • The Nam-Hae Sin-sa, the South Sea shrine in Yeong-Am, Korea was a national institution for public peace and bliss, was excavated in 2000, and the shrine and the 3-way-gate were reconstructed in 2001. Hae Sin-sa, the Sea shrine is a place for religious service separated into the Nam-Hae Sin-sa, the Dong-Hae Myo, and the Seo-Hae Dan. The Dong-Hae Myo was reconstructed, but restored shrine and 3-way-gate of the Nam-Hae Sin-sa is not perfect in comparison with excavation plan in 2000, therefore new reconstruction was researched through the related literature, the analysis of historical maps and excavation results, the interview with the concerned people and the case study. This research defines the analysis of the Plan of the Nam-Hae Sin-sa Reconstruction as follows. 1. The Nam-Hae Sin-sa was the institution for religious service operated by national direct management, represents the shrine for public peace and bliss on the Mountain, the Sea, and the River. Especially the Nam-Hae Sin-sa had an important position on the pivot of international trade with China and Japan, and had a role of main shrine with another one in the Mt. Ji-ri San. 2. The name of the Sea shrine was called as Nam-Hae Sin-sa(the South Sea shrine), Dong-Hae Myo(the East Sea shrine), Seo-Hae Dan(the West Sea shrine). But the name of the South Sea shrine had changed in the early period of Chosun as Nam-Hae Sin-sa to the later Chosun as Nam-Hae Dang through the research of related literature and historical map. Such as the Seo-Hae Dan, it was constructed for the Dan, the flat raised-floor without buildings, and changed to the type of Sa-Dang with addition of buildings. 3. The historical map of Hae Sin-sa informs the types of the roof, the Mat-bae roof was used in the Dong-Hae Myo, but the Pal-jak roof was showed in the Seo-Hae Dan and the Nam-Hae Sin-sa. 4. According to the analysis of Yong-Ch'uck the unit length, Nam-Hae Sin-sa was reconstructed in the period of Koryo on large scale, but it was restored in the Chosun on middle scale. And the Unit of Yong Ch'uck was changed into Yeong-jo Ch'uck in the period of Chosun. 5. As the results, The Plan of the Nam-Hae Sin-sa Reconstruction designed the new shrine into the 3 Kan front and the 2 Kan side with 3:2 scale. An-ch'o-gong with Yong-du and Yong Mi the ornaments represents head and tail of dragon, the Un-gong and the ornament of Pa-ryun-dae-gong in the building, and the Ch'ung-ryang of the Yong-du show the image of the institution for religious service for the god of the sea who look like dragon. The inner gate building and the main entrance were designed as same plan and scale as Hyang-gyo, the Korean Traditional School and Shrine of Confucianism, on the basis of results of excavation. Raise the 3-tall gate of the main entrance with harmony of the scale and the shape, because the side of gate building has the Mat-bae roof. 6. This research shows that Plan of the Nam-Hae Sin-sa Reconstruction is composed into shrine space and reservation space from the main entrance to inner gate and shrine like Jung-ak Dan in the Mt. Gye-ryong San, and it also informs the well in the west side of Sin-sa is an important factor of the plan of shrine architecture.

Numerical analysis study of reinforced method (loop type) at the double-deck tunnel junction (복층터널 분기부에서의 보강공법(루프형 강선)에 따른 수치해석 연구)

  • Lee, Seok Jin;Park, Skhan;Lee, Jun Ho;Jin, Hyun Sik
    • Journal of Korean Tunnelling and Underground Space Association
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    • v.20 no.5
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    • pp.823-837
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    • 2018
  • Congestion of the city with the rapid industrial development was accelerated to build complex social infrastructure. And numerous structures have been designed and constructed in accordance with these requirements. Recently, to solve complex urban traffic, many researches of large-diameter tunnel under construction downtown are in progress. The large-diameter tunnel has been developed with a versatile double-deck of deep depth tunnel. For the safe tunnel construction, ground reinforcement methods have been developed in the weakened pillar section like as junction of tunnel. This paper focuses on evaluation of the effects of new developed ground reinforcement methods in double-deck junction. The values of reinforcement determined from the existing and developed methods were compared to each other by numerical simulation.

Extraction of Glycosaminoglycan from Sea Hare, Aplysia kurodai, and Its Functional Properties 2. Structural Properties of Purified Glycosaminoglycan (군소(Aplysia kurodai)에 분포하는 글루코사미노글리칸의 추출과 기능특성 2. 글루코사미노글리칸의 구조 특성)

  • Yoon, Bo-Yeong;Choi, Byeong-Dai;Bae, Dong-Won;Choi, Yeung-Joon
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.39 no.11
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    • pp.1647-1653
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    • 2010
  • Glycosaminoglycan (GAG) was purified from polysaccharide extracted from sea hare muscle on DEAE-Sepharose column and investigated for the functional groups, distribution of sugars, composition of disaccharide and structure of GAG. Purified GAG was composed of disaccharide above 55% of total sugar. Purified GAG showed amide I peak in 1648/cm and C-O stretch peak as properties of carbohydrate, amino acid peak in 1457/cm, and peak in 866/cm as properties of monosaccharide by FT-IR. Fucose, N-acetylgalactosamine, N-acetylglucosamine, glucose, galactose, mannose and xylose were found in MALDI-TOF MS/MS spectra of hydrolysates by chondroitin sulfate ABC lyase and heparanase I. Purified GAG was expected to be heparan sulfate including N-acetylgalactosamine and N-acetylglucosamine above 70% of total sugar. The structure of GAG was supposed as GlyUA(2S)-GlcNS and GlyUA-GlcNS(6S) with O-linkage on protein core.

GPR Analysis on Underground Features and Foundation Structure of Cheomseongdae, Gyeongju (GPR 탐사를 통해 본 경주 첨성대 기초 및 주변의 유구 분석)

  • Oh, Hyundok;Kwon, Moonhee;Jang, Hangilro
    • Korean Journal of Heritage: History & Science
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    • v.52 no.4
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    • pp.264-271
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    • 2019
  • Cheomseongdae in Gyeongju, known as an astronomical observatory, is a cultural monument with great historical, academic, and artistic value, as its unique shape is preserved well in its original form. The outer structure, ground stability, and seismic reliability of Cheomseongdae have been assessed by numerous researchers through various scientific methods, but research on the underground structure has been insufficient. This paper contains detailed models of the underground structure of Cheomseongdae interpreted in 2D and 3D images based on the data acquired through GPR surveys conducted of features in and around the base of Cheomseongdae. As a result, the existence of twelve small features arranged in a circle, although only about half of them remain, was confirmed at a depth of 0.4 - 0.6m. Furthermore, a structure three bays long (north-south direction) and four bays wide (east-west direction) was detected beneath Cheomseongdae at the depth of 0.7 - 1.0m. Other than 2 layers of foundations as is known, a square structure with the dimensions of 7m × 7m is situated at a depth of 0.6m, directly under Cheomseongdae, and what is reading that is expected to be the foundation structure of Cheomseongdae was detected and confirmed. This foundation structure is circular with a diameter in the east-west direction of 11m and in the north-south direction of 12m. The northern, western, and eastern edges of this foundation structure are about 1m away from the foundation of Cheomseongdae, whereas the the south side extends to about 5m wide.