• The korean journal of orthodontics
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• v.32 no.3 s.92
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• pp.227-234
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• 2002

In order to investigate the action mechanism of protein kinase C on $K^+$ channel in osteoblastic cell, effects of phorbol 12, 13-dibutyrate on human osteoblast-like cells (G292) were studied by patch clamp technique with cell-attacked configuration. 111 this experiment, 45pS ion channel was dominant in G292 cell line according to their approximate conductances in symmetrical 140mM KCl saline at holding potential of 60mV. In torrent-voltage relationship, reversal potential was 5.5mV at the condition of potassium enriched saline in the pipette and -27 mV at the condition of standard extracellular saline In the pipette. Phorbol 12, 13-dibutyrate 10nM increased the open probability of 45pS channel and staurosporine, an inhibitor of protein kinase C, suppressed this effect. Phorbol 12,13-dibutyrate moved the reversal potential of 45pS channel to more negative potential and increased the single channel current at the same membrame potential. In order to check the activation of protein kinase C in G292 cell by phorbol 12,13-dibutyrate, western blot of protein kinase C was performed. Phorbol 12,13-dibutyrate $0.1{\mu}M$ translocated protein kinase C from cellular compartment to membrane compartment of the cell. These findings suggest that phorbol 12,13-dibutyrate, one of phorbol esters, activate 45pS channel In G292 cell and affect cell membrane potential, that regulate cellular function.

• Aerospace Industry
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• s.107
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• pp.52-53
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• 2010

• 한국박물관협회:학술대회논문집
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• v.1999 no.05
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• pp.13-16
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• 1999

• Proceedings of the Korea Information Processing Society Conference
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• 2013.05a
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• pp.524-526
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• 2013

본 논문은 현재 이슈화 되고 있는 CCTV 에서 광역 감시를 위한 지능형 영상보안 기술에 대해 서술한다. 광역영상보안시스템은 단일 CCTV 에서 일부 지역에 대한 감시를 벗어나 지역과 지역을 연계하여 보다 넓은 지역을 하나의 시스템으로 연동하여 신변의 안전 서비스를 제공하는데 목적을 갖는다. 본 시스템에서는 광역영상보안시스템 환경에서 요구하는 지능형 영상보안 기술로써 마스킹 기술, 이벤트 탐지 기술, 그리고 연동 기반의 객체 추적 기술, 객체 검색 기술 및 증거영상 생성 기술을 제시한다.

• Journal of the Institute of Electronics Engineers of Korea SC
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• v.38 no.4
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• pp.20-30
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• 2001

A novel 200 MHz CMOS transconductor using translinear cells is proposed. The proposed transconductor consists of voltage followers and current followers based on translinear cells and a resistor. For wide applications, a single-input single-output, a single-Input differential-output, and a fully-differential transconductor are systematically designed, respectively. The theory of operation is described and computer simulation results are used to verify theoretical predictions. The results show that the fully-differential transconductor has a linear input voltage range of ${\pm}2.7$ V, a 3 dB frequency of 200 MHz, and a temperature coefficient of less than 41 $ppm/^{\circ}C$ at supply voltages of ${\pm}3$ V. In order to certify the applicability of the fully-differential transconductor, A ladder-type 3th-order cllitic low pass filter is also designed based on the inductance simulation method. The filter has a ripple bandwidth of 22 MHz, a pass-band ripple of 0.36 dB, and a cutoff frequency of 26 MHz.

• Proceedings of the Korean Society of Developmental Biology Conference
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• 1998.07a
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• pp.53-54
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• 1998

햄스터 난자에서 관찰되는 SAC의 활동은 30mmHg 이내의 음압자극에 대하여 잘 관찰되었으며 단일통로의 크기나 open time에 따라 4종류로 구분될 수 있었다. SAC 활동은 PKA에 의하여 활성이 조절되었으며 수정 후 할구분할이 진행될수록 SAC 전도도가 작은 통로로 전환되어 발생 초기 SAC 활도양상이 특징적인 변화가 관찰되었다.

• Journal of Embryo Transfer
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• v.19 no.2
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• pp.101-112
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• 2004

Chloride($Cl^-$) channels play critical roles in cell homeostasis and its specific functions such as volume regulation, differentiation, secretion, and membrane stabilization. The presence of these channels have been reported in all kinds of cells and even in frog oocytes. These essential role of $Cl^-$­ channels in cell homeostasis possibly play any role in egg homeostasis and in the early stage of development, however, there has been no report about the presence of $Cl^-$­ channel in the mammalian oocyte. This study was performed to elucidate the presence of $Cl^-$­ channels in hamster eggs. When allowing only $Cl^-$­ to pass through the channel of the egg membrane by using impermeant cation such as N-methyl-D-glucamine(NMDG), single channel currents were recorded. These channel currents showed typical long-lasted openings interrupted by rapid flickering. Mean open $time({\tau}o)$ was 43${\pm}$10.14 ms(n=9, at 50 mV). The open probability(Po) was decrease with depolarization. The current-voltage relation showed outward rectification. Outward slop conductance(32${\pm}$5.4 pS, n=22) was steeper than the inward slop conductance(10${\pm}$1.3 pS). Under the condition of symmetrical 140 mM NaCl, single channel currents were reversed at 0 mV(n=4). This reversal potential(Erev) was shifted from 0 mV at 140 mM concentration of internal NaCl(140 mM [Na+]i) to ­9.8${\pm}$0.5 mV(n=4) at 70 mM [Na+]i and 11.5${\pm}$1.9 mV at 280 mM [Na+]i(n=4) respectively, strongly suggesting that these are single $Cl^-$­ channel currents. To examine further whether this channel has pharmacological property of the $Cl^-$­ channel, specific Cl­ channel blockers, IAA-94(Indanyloxyacetic acid-94) and DIDS(4, 4'-diisothiocyan ostillben- 2-2'disulfonic acid) were applied. IAA-94 inhibited the channel current in a dose-dependent manner and revealed a rapid and flickering block. From these electrophysiological and pharmacological resluts, we found the novel $Cl^-$­ channel present in the hamster oocyte membrane. The first identification of $Cl^-$­ channel in the hamster oocyte may give a clue for the further study on the function of $Cl^-$­ channel in the fertilization and cell differentiation.

• Proceedings of the Korean Nuclear Society Conference
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• 1996.05d
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• pp.327-332
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• 1996

대구경유압식스너버의 잠재적 기능상실은 미국원자력규제위원회(USNRC)에서 인정한 주요안 전성 문제중의 하나이다. 본 보고서는 한국표준형원전의 증기발생기와 원자로냉각재펌프에 설치되어있는 대구경유압식스너버 중 단일 스너버의 기능상실에 대하여 지진과 가상분기관파단의 시간이력해석을 수행하여 구조적영향을 보였다. 지진 입력은 SRP 3.7.1 에 따른 가상적 시간이력이며, 분기관파단 입력은 파단전누설기법(Leak Before Break) 적용에 의하여 배제되지 못한 분기관파 단인 증기발생기 주증기배관과 증기발생기 주급수배관의 가상파단을 이용하였다.

• 건축구조
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• v.11 no.2
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• pp.23-29
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• 2004

우리회는 한국기술사회건축구조분회로 출발하여 2년 전 유병억 회장님시절 개인사업자등록증을 발급받아 구조분회가 아닌 개인 사업체로서 한걸음 진일보하였다가 작년에야 비로서 우리의 숙원이었던 과학기술부 산하 사단법인체로 등록되면서 명실공히 사단법인 한국건축구조기술사회로 위상을 한단계 격상함으로서 합법적으로 정부로부터 인가받은 단체로 발돋음하였다. 그동안 건축구조안전에 대한 문제점을 알고 있었으나, 정부와의 어떠한 합법적이고 독립적인 통로가 없어 제대로된 의견개진을 할 수 없었던 상황에서 이제는 올바른 통로를 확보한 첫 걸음이라고 판단된다. 아직도 우리가 헤쳐나아가야 할 길은 멀고도 험난한 상황임을 잊지말고 끈기있는 인내심과 중단없는 전진을 통해 하나씩 건축물의 구조안전을 확보하기 위한 방안을 위해 지속적으로 노력을 하였으면 한다. 올해 2월 정기총회를 통해 사단법인으로 처음 출발하면서 이동헌회장님을 비롯한 신임회장단이 선출되었고, 그 장도의 첫발을 내딛으면서 앞으로 우리가 어떤 목표를 견지하여야 할 것인가에 대해 의식고취 및 앞으로의 방향설정을 위해 지난해 3월 건설교통부에서 발족한 건설교통안전기획단의 검토 내용에서 발췌한 현행 실태와 문제점 및 대책 중에서 건축물의 구조안전과 관련된 문제점을 다시 한번 되새기면서 우리의 목표와 방향을 점검하는 계기가 되었으면 한다.

• Journal of the Korean Institute of Telematics and Electronics C
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• v.36C no.1
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• pp.49-58
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• 1999

This paper describes an integrated low pass filter fabricated by using $0.8{\mu}m$ single poly CMOS ASIC technology. The filter has been designed for a 5th-order elliptic switched capacitor filter with cutoff frequency of 5khz, 0.1dB passband ripple. The filter consists of MOS swiches poly capacitors and five CMOS op-amps. For the realization of the SC filter, continuous time transfer function H(s) is obtained from LC passive type, and transfered as discrete time transfer H(z) through bilinear-z transform. Another filter has been designed by capacitor scaling for reduced chip area, considering dynamic range of the op-amp. The test results of two fabricated filters are cutoff frequency of 4.96~4.98khz, 35~38dB gain attenuation and 0.72~0.81dB passband ripple with the ${\pm}2.5V$power supply clock of 50KHz.