• Proceedings of the Korean Society of Applied Pharmacology
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• 1994.04a
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• pp.185-185
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• 1994

관상동맥은 전기적으로 자극하여도 활동전위가 쉽게 유발되지 않는 조직인데, 이는 세포내의 $Ca^{2+}$에 의해서 활성화되는 $K^{+}$통로때문이라고 여겨지고 있다. 저자들은 단일 이온통로 수준에서 이러한 관상동맥 $K^{+}$ 통로의 성질을 조사하고, 아울러 $K^{+}$ 통로 개방제를 포함한 몇가지 약물이 $K^{+}$ 통로에 미치는 영향을 알아보고자 본 연구를 수행하였다. 막소포는 돼지의 심장에서 관상동맥을 분리하여 균질화 및 원심분리하여 준비하였으며, sucrose 20/30%에 있는 층을 취하여 분주한 후 -7$0^{\circ}C$로 보관하여 사용하였다. 평지방막은 1-palmitoyl, 2-oleoyl-phosphatidyl ethanolamine과 1-palmitoyl, 2-oleoyl phosphatidylcholine (4:1)을 n-decane에 녹인지방액(25mg/m1)을 200 $\mu\textrm{m}$의 구멍에 칠하여 만들었다. 단일 $K^{+}$ 통로는 막소포 (1-5$\mu\textrm{g}$/ml)를 평지방막의한쪽에 추가하고 자석으로 저어 융합이 되도록 하였으며, 통로의 활성은 막의 양쪽에 150 mM KCI, 10 mM HEPES-KOH, 0.5 mM EGTA, 0.6 mM $CaCl_2$ (pH 7.2)로 된용액이 존재하는 상태에서 막전압을 다르게 하며 기록하였다. 단일 이온 통로 전류는 amplifier를 통하여 VCR tape에 녹화하여 후에 분석에 이용하였으며, 동시에 종이기록지에 기록하였다. 단일이온통로 활성의 분석은 pClamp (Ver5.5)를 이용하여 수행하였다. 관상동맥에서 가장 흔히 기록되는 이온통로는 막전압과 $Ca^{2+}$의 농도에 의하여 변하는 $K^{+}$ 통로이었는데 pCa 4.0과 -10 mV에서 초기 활성도(Po)는 0.1에서 0.8까지 다양하였다. 전류와 전압과의 관계는 -60 - +60mV의 전압범위에서 직선형이었다.

• Restorative Dentistry and Endodontics
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• v.27 no.5
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• pp.451-462
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• 2002

삼차신경은 구강악안면영역의 운동 및 감각을 담당하고 있으므로 치과임상에서 매우 중요하다. 삼차신경근 중 삼차신경절에 세포체를 갖는 뉴론은 주로 체성 감각을 전달하는 1차 구심신경으로 악안면영역의 촉각, 압각, 진동감각 온도각 및 통각을 담당한다. 이러한 감각의 전달은 기본적으로 신경세포의 이온통로의 활동에 의존하는데 삼차신경절 세포에 여러 종류의 이온통로가 존재하는 것으로 알려져 있다. 본 연구에서는 항체 염색법을 이용하여 이온통로가 존재를 확인 하고자 한다. 횐쥐의 삼차신경절로부터 통법에 따라 뉴론을 단일 세포로 분리하고 immunocytochemistry 방법으로 세포를 염색하여 관찰한 바 다음과 같은 결과를 얻었다. 본 실험에서 이온전류의 측정 등으로 관찰된 여러 종류의 이온통로들을 면역 염색법으로 확인하였다. 횐쥐의 삼차신경절 뉴론에서 확인된 이온통로는 소디움통로와 N, P 및 Q-type의 칼슘통로 그리고 BK$_{Ca}$, Kv 4.2 및 Kir 2.1 등의 포타슘통로이었으며 이온통로의 종류에 따라 분포에 차이를 나타내었다.

• Journal of Veterinary Clinics
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• v.28 no.6
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• pp.555-561
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• 2011

Laparoscopic surgery is a well-established alternative to open surgery across disciplines. However, in veterinary medicine, laparoscopic surgery in dogs was rarely reported because of small abdominal size for multiple ports insertion. The concept of single-incision laparoscopic surgery (SILS) is to perform the entire laparoscopic operation through a single incision rather than conventional multiple small skin incisions. Indirect evidence of potential benefits of SILS, decreases operative morbidity related to reduction in port size, already exists. Therefore, this study was performed to evaluate the safety and feasibility of the modified form of SILS using flexible endoscope in Cholecystectomy before clinical adoption. A 2 cm single periumbilical incision was performed, and flexible endoscope was introduced into the abdominal cavity. A laparoscopic grasper was inserted into the abdominal cavity for the traction of gall bladder. Cystic duct and artery were ligated by 5 mm Hem-o-lok$^{(R)}$. Then, gall bladder was dissected and resected from the liver with 5 mm Autonomy Laparo-Angle Maryland dissector and endoscopic needle knife. Resected gall bladder was wrapped by using specimen pouch and was retrieved through abdominal incision from the cavity. All three gall bladders were successfully removed. Hematological changes were not observed during examination periods. No leakage sign was identified at necropsy. The flexible endoscope, as distinct from conventional rigid laparoscope, allows the visualization from various angles and the wide range of motion, result in less crowding.

• Progress in Medical Physics
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• v.12 no.1
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• pp.59-70
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• 2001

Adrenal chromaffin cells secrete catecholamine in response to acetylcholine. The secretory response has absolute requirement for extracellular calcium, indicating that $Ca^{2+}$ influx through voltage operated $Ca^{2+}$ channels is the primary trigger of the secretion cascade. Although the existence of various types of $Ca^{2+}$ channels has been explored using patch clamp technique in adrenal chromaffin cells, there is still disagreement with the types of $Ca^{2+}$ channels existed in different species. Therefore, we have tried to identify several distinct types of $Ca^{2+}$ channels in rat chromaffin cells. By using nicardipine(L type channel blocker), $\omega$-CgTx GVIA(N type channel blocker), and $\omega$-AgaTx VIA(P type channel blocker), it was identified that L, N, and P type $Ca^{2+}$ channel exist in rat adrenal chromaffin cells and the order of contribution of each channel type to whole cell $Ca^{2+}$ current was L type> N type> P type. type> P type.

• Journal of Astronomy and Space Sciences
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• v.21 no.2
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• pp.153-166
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• 2004

Interplanetary trajectories using the gravity assists are studied for future Korean interplanetary missions. Verifications of the developed softwares and results were performed by comparing data from ESA's Mars Express mission and previous results. Among the Jupiter exploration mission scenarios, multi-planet gravity assist mission to Jupiter (Earth-Mars-Earth-Jupiter Gravity Assist, EMEJGA trajectory) requires minimum launch energy ($C_3$) of 29.231 $Km^2$/$S^2$ with 4.6 years flight times. Others, such as direct mission and single-planet(Mars) gravity assist mission, requires launch energy ($C_3$) of 75.656 $Km^2$/$S^2$ with 2.98 years flight times and 63.590 $Km^2$/$S^2$ with 2.33 years flight times, respectively. These results show that the planetary gravity assists can reduce launch energy, while EMEJGA trajectory requires the longer flight time than the other missions.

• Fire Science and Engineering
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• v.23 no.5
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• pp.143-152
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• 2009

The numerical analyses for each single simplified shaft with three type openings were carried out by using computational fluid dynamics model for the calculation of the pressure difference and the location of the neutral plane and the visualization of stack effect. As the height of shaft heighten, the pressure difference of stack effect is much deviated against the theoretical value. For the Type A models shorter than 30 m height of shaft and the Type B models longer than 30m, the simulation results for the location of the neutral plane are well agreed to the theoretical values with 5% less deviations just after the beginning of simulation (t = 10s). For the Type B models longer than 30m with multiple openings, therefore, it is possible to calculate the location of the neutral plane by using a CFD model. The phenomenon of the air flow of stack effect can be easily understood with the visualization of stack effect.

• The Journal of the Convergence on Culture Technology
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• v.7 no.2
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• pp.419-425
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• 2021

This study analyzed the safety of evacuation according to the number of exits and the direction of evacuation routes with evacuation simulations of the Pathfinder in case of a fire at a highway service area. It was also analyzed of the difference in RSET by comparing the single or double types of the exit of a facility. The results were as follows. When only one direction exit was opened, all of the RSET were exceeded. When two or more different directions were opened according to the general principle of evacuation, all results met the RSET. The simulations showed that two or more different directions were more shortened in RSET than the one same direction. The result of the types of doors showed that the single type door was shortened in RSET rather than the double type doors. For the evacuation safety in a fire at the highway service areas, firstly, it is necessary to secure two or more exits in a fire at all times. Secondly, the exits should not be same directions. Finally, it is suggested that the exit should be installed with a single type of door.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.19 no.12
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• pp.2943-2948
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• 2015

Run 3D reconstruction using a single camera, based on the information, we are advancing research on driving assistance apparatus or can be informed how to pass the obstacle existing ahead the driver. As a result depth information falls but it is possible to provide information that can pass through an obstacle on the straight. For 3D reconstruction by measuring the internal parameters, it calculates the Fundamental matrix and matching to find the feature points obtained by executing the triangulation on the basis of this. When the through experiments try to confirm the results, the depth information is present error information in the X and Y axes which can determine whether or not to pass through an obstacle has reliability.

• The Korean Journal of Applied Statistics
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• v.12 no.1
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• pp.1-15
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• 1999

수산물 비계통 생산량 조사는 수산물 생산량 조사 중 어가부분에서 비계통 출하된 양에 대한 표본조사이다. 본연구는 1995년 어업 총조사 자료에 근거하여 새로운 표본 설계를 제안하는 것을 목적으로 한다. 표본은 층화 2단 집락 추출방식에 의해 추출되었으며 층화변수로는 어업조사구 내의 일반어류 어가 비율을 사용하였다.

• Journal of Power System Engineering
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• v.8 no.4
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• pp.5-10
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• 2004

본 연구는 단기통 4행정 기관의 배기단의 형상에 따른 실험과 수치해석이 소개되었다. 흡 배기 밸브가 작동하고 있고, 주요한 배기단의 변수로는 배기단 직경이 적용이 되었다. 실험결과로는 배기단의 직경에 따라 배기 압력은 많은 영향을 받았는 것으로 나타났다. 배기단의 직경이 감소하였을때, 배기압력파의 진폭과 파수가 증가되었다. 배기단의 직경이 증가 하였을 때, 배기압력파의 진폭이 감소하였다. 직경이 22mm 일 때의 소음의 주파수 분석이 16mm와 28mm 보다 진폭이 작게 나타났다.